ANAT2341 Lab 4

From Embryology

1. QUIZ

2. Guest Lecturer - Dr Fabien Delerue

Fabien deleRue profile photo.jpg

Dr Fabien Delerue

Manipulating the mouse embryo: from ES Cells to genome editing


Doctor Fabien Delerue is the Head of the Genome Editing at Macquarie (GEM), a transgenic core developed in joint venture by the Dementia Research Centre (DRC) and the Macquarie Animal Research Services (MARS). Fabien graduated from the University of Bordeaux in France and received his PhD from the University of Sydney in 2013. Fabien gained his first research experience in the preclinical CNS Centre of Hoffmann-La Roche Ltd (Basel, Switzerland) where he became an expert in in vivo activities (i.e. animal models of diseases). In 2013, Fabien moved to the University of New South Wales, where he became Lecturer and managed the Transgenic Animal Unit. His research focused on developing new gene transfer techniques particularly applicable to dementia and rare genetic diseases. In 2018 he became Senior Lecturer and joined Macquarie University to head the GEM transgenic facility and develop in parallel a research program on rare genetic disorders. Research interests

Fabien’s contribution to the field of transgenesis and genome editing is internationally recognized. Fabien’s career spans over 20 years practice in assisted reproduction and generation of animal models of diseases. Current projects include:

- Optimization of gene transfer methods for the generation of transgenic animal models

- Development of new assisted reproductive techniques

- Discovery and engineering of new endonucleases

- Investigating the pathomechanisms of FoxG1 syndrome

Lecture slides: File:Delerue.pdf

References

Delerue F & Ittner LM. Genome Editing in Mice Using CRISPR/Cas9: Achievements and Prospects Cloning and Transgenesis 2015, 4:135 doi:10.4172/2168-9849.1000135 [1]

"Animal models are a powerful tool to understand the mechanisms underlying physiological and pathological processes in vivo. To date, mice remain the species most commonly used for genetic manipulation. The recent development of engineered endonucleases such as Zinc Finger Nucleases (ZFN), Transcription activator-like effector nucleases (TALEN), and the Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR/Cas9) offered easy, flexible, and fast alternative to ES-Cell based gene targeting. Thanks to multiple advantages, the CRISPR system superseded its predecessors and became a popular method for genome editing. Here, we review the latest techniques to apply CRISPR editing to the mouse genome, and emphasize on the current methods used in transgenic laboratories and subsequent achievements in mice."

Ke YD, van Hummel A, Stevens CH, Gladbach A, Ippati S, Bi M, Lee WS, Krüger S, van der Hoven J, Volkerling A, Bongers A, Halliday G, Haass NK, Kiernan M, Delerue F, Ittner LM.
Short-term suppression of A315T mutant human TDP-43 expression improves functional deficits in a novel inducible transgenic mouse model of FTLD-TDP and ALS. Acta Neuropathologica 2015 Nov;130(5):661-78 PubMed 26437864 [2]

Ittner A, Chua SW, Bertz J, Volkerling A, van der Hoven J, Gladbach A, Przybyla M, Bi M, van Hummel A, Stevens CH, Ippati S, Suh LS, Macmillan A, Sutherland G, Kril JJ, Silva APG, Mackay J, Poljak A, Delerue F, Ke YD, Ittner LM. Site-specific phosphorylation of tau inhibits amyloid-β toxicity in Alzheimer’s mice Science 2016 Nov;354 (6314):904-908 doi: 10.1126/science.aah6205 [3]

Recommended Literature

Manipulating the Mouse Embryo.jpg Reproductive Engineering Techniques.jpg
Manipulating the Mouse Embryo: A Laboratory Manual (Fourth Edition) By Richard Behringer, Marina Gertsenstein, Kristina Vintersten Nagy, & Andras Nagy. Reproductive Engineering techniques in mice: Technical manual Prof Naomi Nakagata, Center for Animal Resources and Development, Kumamoto University, Japan

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