Zebrafish Development: Difference between revisions

From Embryology
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| 10.33 - 24 hrs  ||'''[[Segmentation Period]]''' ||[[File:Segmentation .png|The Segmentation Period. Photo supplied by Judy Cebra-Thomas]]
| 10.33 - 24 hrs  ||'''[[Segmentation Period]]''' ||[[File:Segmentation .png|The Segmentation Period. Photo supplied by Judy Cebra-Thomas]]
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| 24 - 48 hrs  || '''[[Pharyngula Period]]''' || [[File:Pharyngula.png|The Pharyngula Period. Photo supplied by Judy Cebra-Thomas]]
| 24 - 48 hrs  || '''[[#Pharyngula Period|Pharyngula Period]]''' || [[File:Pharyngula.png|The Pharyngula Period. Photo supplied by Judy Cebra-Thomas]]
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| 48-72 hrs  ||'''[[Hatching Period]]''' ||[[File:Hatching_.png|The Hatching Period. Photo supplied by Judy Cebra-Thomas‎]]
| 48-72 hrs  ||'''[[Hatching Period]]''' ||[[File:Hatching_.png|The Hatching Period. Photo supplied by Judy Cebra-Thomas‎]]
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===Pharyngula Period===
* Transition from Prim 5 to Long-pec
* The body axis begins to straighten and the head straightens out and lifts dorsally
* Notochord is well developed
* Formation of the Dorsal and Ventral Stripe
* Nervous system is hollow and expanding anteriorly
* The brain has developed into 5 distinct lobes
* Seven pharyngeal arch's develop rapidly during this stage
* Pectoral fins begin to develop
* The Circulatory system develops and the heart beats for the first time
* Blood begins to circulate through a closed circuit of channels
* Tactile sensitivity appears and uncoordinated movements occur
<gallery>
File:Zebrafish day 1 SEM.jpg|day 1
File:Zebrafish brain fold SEM.jpg|brain fold
File:Zebrafish myotomes SEM.jpg|myotomes
File:Zebrafish trunk SEM01.jpg|trunk
File:Zebrafish trunk SEM02.jpg|trunk
File:Zebrafish perichordal sheath SEM.jpg|perichordal sheath
File:Zebrafish enveloping layer SEM01.jpg|enveloping layer
File:Zebrafish enveloping layer SEM02.jpg|enveloping layer
<gallery>


==Skull==
==Skull==

Revision as of 15:40, 12 December 2014

Embryology - 28 Mar 2024    Facebook link Pinterest link Twitter link  Expand to Translate  
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Introduction

Zebrafish-icon.png

Zebrafish or zebra danio (danio rerio) are seen as the latest "model' for embryological development studies. These embryos have the great advantage that they develop as "see through" embryos, that is, all internal development can be clearly observed from the outside in the living embryo. Much of the early modern work using this embryo model began with the papers of Kimmel.[1][2]

Several large laboratories in the US are now developing large breeding programs to carry out "knockouts" and to find spontaneous mutants of interest.


Fish Links: Zebrafish Development | Medaka Development | Salmon Development | Movie - Zebrafish Heart | Student Group Project - Zebrafish | Recent References | Category:Zebrafish | Category:Medaka

Some Recent Findings

Nipbl heart and organ patterning[3]
  • Construction of a vertebrate embryo from two opposing morphogen gradients[4] "Here, we show that opposing gradients of bone morphogenetic protein (BMP) and Nodal, two transforming growth factor family members that act as morphogens, are sufficient to induce molecular and cellular mechanisms required to organize, in vivo or in vitro, uncommitted cells of the zebrafish blastula animal pole into a well-developed embryo." BMP
  • FishFace: interactive atlas of zebrafish craniofacial development at cellular resolution[5] "We present the Fish Face Atlas, an online, 3D-interactive atlas of craniofacial development in the zebrafish Danio rerio. Alizarin red-stained skulls scanned by fluorescent optical projection tomography and segmented into individual elements provide a resource for understanding the 3D structure of the zebrafish craniofacial skeleton." See also Online Zebrafish Atlases
  • Multifactorial Origins of Heart and Gut Defects in nipbl-Deficient Zebrafish, a Model of Cornelia de Lange Syndrome[3] "Cornelia de Lange Syndrome (CdLS) is the founding member of a class of multi-organ system birth defect syndromes termed cohesinopathies, named for the chromatin-associated protein complex cohesin, which mediates sister chromatid cohesion. Most cases of CdLS are caused by haploinsufficiency for Nipped-B-like (Nipbl), a highly conserved protein that facilitates cohesin loading. ... These findings support the view that birth defects in CdLS arise from collective effects of quantitative changes in gene expression. Interestingly, both the phenotypes and gene expression changes in nipbl morphants differed from those in mutants or morphants for genes encoding cohesin subunits, suggesting that the transcriptional functions of Nipbl cannot be ascribed simply to its role in cohesin loading. (OMIM - CDLS1 | CDLS2 | CDLS3)
  • The zebrafish transcriptome during early development[6] "The three earliest developmental stages were similar when comparing highly expressed genes, whereas the 50% epiboly stage differed from the other three stages in the identity of highly expressed genes, number of uniquely expressed genes and enrichment of GO molecular functions. Taken together, these observations indicate a major transition in gene regulation and transcriptional activity taking place between the 512-cell and 50% epiboly stages, in accordance with previous studies."
  • Genetic analysis of fin development in zebrafish identifies furin and hemicentin1 as potential novel fraser syndrome disease genes[7] " Three of them are due to mutations in zebrafish orthologues of FRAS1, FREM1, or FREM2, large basement membrane protein encoding genes that are mutated in mouse bleb mutants and in human patients suffering from Fraser Syndrome, a rare congenital condition characterized by syndactyly and cryptophthalmos. Fin blistering in a fourth group of zebrafish mutants is caused by mutations in Hemicentin1 (Hmcn1), another large extracellular matrix protein the function of which in vertebrates was hitherto unknown. Our mutant and dose-dependent interaction data suggest a potential involvement of Hmcn1 in Fraser complex-dependent basement membrane anchorage. Furthermore, we present biochemical and genetic data suggesting a role for the proprotein convertase FurinA in zebrafish fin development and cell surface shedding of Fras1 and Frem2, thereby allowing proper localization of the proteins within the basement membrane of forming fins."
More recent papers
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Search term: Zebrafish Embryology

<pubmed limit=5>Zebrafish Embryology</pubmed>

Timeline and Stages of Embryonic Development

Duration Period Name Image
0 - 0.75 hrs Zygote Period The Zygote Period. Photo supplied by Judy Cebra-Thomas
0.75 - 2.25 hrs Cleavage Period The Cleavage Period. Photo supplied by Judy Cebra-Thomas
2.25 - 5.25 hrs Blastula Period The Blastula Period. Photo supplied by Judy Cebra-Thomas‎
5.25 - 10.33 hrs Gastrula Period The Gastrula Period. Photo supplied by Judy Cebra-Thomas
10.33 - 24 hrs Segmentation Period The Segmentation Period. Photo supplied by Judy Cebra-Thomas
24 - 48 hrs Pharyngula Period The Pharyngula Period. Photo supplied by Judy Cebra-Thomas
48-72 hrs Hatching Period The Hatching Period. Photo supplied by Judy Cebra-Thomas‎
72 hrs - 30 Days Larval Period The Larval Period. Photo supplied by Judy Cebra-Thomas‎

Pharyngula Period

  • Transition from Prim 5 to Long-pec
  • The body axis begins to straighten and the head straightens out and lifts dorsally
  • Notochord is well developed
  • Formation of the Dorsal and Ventral Stripe
  • Nervous system is hollow and expanding anteriorly
  • The brain has developed into 5 distinct lobes
  • Seven pharyngeal arch's develop rapidly during this stage
  • Pectoral fins begin to develop
  • The Circulatory system develops and the heart beats for the first time
  • Blood begins to circulate through a closed circuit of channels
  • Tactile sensitivity appears and uncoordinated movements occur


Terms

  • Kupffer's vesicle - (ciliated organ of asymmetry, primitive node) a transient epithelial fluid-filled sac located midventrally posterior to the yolk cell or its extension. The vesicle has been described as equivalent to the primitive node for establishing embryo left-right (L-R) axis. PMID 21876750

External Links

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Cite this page: Hill, M.A. (2024, March 28) Embryology Zebrafish Development. Retrieved from https://embryology.med.unsw.edu.au/embryology/index.php/Zebrafish_Development

What Links Here?
© Dr Mark Hill 2024, UNSW Embryology ISBN: 978 0 7334 2609 4 - UNSW CRICOS Provider Code No. 00098G
  1. <pubmed>7229136</pubmed>
  2. <pubmed>3077108</pubmed>
  3. 3.0 3.1 <pubmed>22039349</pubmed>
  4. <pubmed>24700857</pubmed>
  5. <pubmed>23714426</pubmed>
  6. <pubmed>21609443</pubmed>
  7. <pubmed>20419147</pubmed>