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== Lab 6 Assessment ==  
== Lab 6 Assessment ==  


[http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4887708/ Parental cigarette smoking, transforming growth factor-alpha gene variant and the risk of orofacial cleft in Iranian infants]
The deletion or mutation of the epidermal growth factor receptor at the medial edge epithelium where the Transforming Growth Factor a is expressed is one of the identified genetic mutations that results in cleft palate.
PMID 4887708


Identify Known Genetic Mutation: The deletion or mutation of the epidermal growth factor receptor at the medial edge epithelium where the Transforming Growth Factor a is expressed is one of the identified genetic mutations that results in cleft palate.
A recent research article on this gene: [http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4887708/ Parental cigarette smoking, transforming growth factor-alpha gene variant and the risk of orofacial cleft in Iranian infants]
 
A recent research article on this gene: Parental cigarette smoking, transforming growth factor-alpha gene variant and the risk of orofacial cleft in Iranian infants


How does this mutation affect developmental signalling in normal development:  
How does this mutation affect developmental signalling in normal development:  
TGFA is concentrated in the epithelial tissue located in the medial edges of palatal shelves. In normal development, this gene assists in the production of extracellular matrix and also enables the palate to fuse without disruption by promoting normal movement and positioning of mesenchymal cells. Three mutations of TGFA associated with cleft palate are RsaI, and TaqI in intron 5 and BamHI in exon 6. When this gene is mutated, an abnormal or incomplete formation of the palate occurs.
TGFA is concentrated in the epithelial tissue located in the medial edges of palatal shelves. In normal development, this gene assists in the production of extracellular matrix and also enables the palate to fuse without disruption by promoting normal movement and positioning of mesenchymal cells. Three mutations of TGFA associated with cleft palate are RsaI, and TaqI in intron 5 and BamHI in exon 6. When this gene is mutated, an abnormal or incomplete formation of the palate occurs.

Revision as of 23:50, 15 September 2016

Lab Attendance

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Internal Link

Student Information (expand to read)  
Individual Assessments
Mark Hill.jpg

Please leave this template on top of your student page as I will add your assessment items here.

Beginning your online work - Working Online in this course

  1. Make your own page.
    1. Log-in to the embryology website using your student ID and Zpass.
    2. Click your student number (shown in red at the top right of the screen following log-in)
    3. Create page using the tab at the top of the page, and save.
  2. Add the following to the top of your page exactly as shown - {{ANAT2341Student2016}}
  3. How would you identify your Type in a group and add to your page.
  4. What was the most interesting thing you learnt in the fertilisation lecture?


If you have done the above correctly your ZID should be blue and not red on this page link - ANAT2341 2016 Students.


Here is the example page I made in Lab 1 Student Page. With a few more explanatory notes.

Click here to email Dr Mark Hill

Editing Links: Editing Basics | Images | Tables | Referencing | Journal Searches | Copyright | Font Colours | Virtual Slide Permalink | My Preferences | One Page Wiki Card | Printing | Movies | Language Translation | Student Movies | Using OpenOffice | Internet Browsers | Moodle | Navigation/Contribution | Term Link | Short URLs | 2018 Test Student
Lab 1 Assessment - Researching a Topic
In the lab I showed you how to find the PubMed reference database and search it using a topic word. Lab 1 assessment will be for you to use this to find a research reference on "fertilization" and write a brief summary of the main finding of the paper.
  1. Add a new Sub-heading "Lab 1 Assessment" (without the quotes).
  2. Search the database for a reference on "fertilisation" published in the last 5 years.
    1. It must be a research article not a Review.
    2. The full paper must be available online, not just the abstract.
  3. Add a link to this reference using its PMID using this code <pubmed>XXXXX</pubmed> replacing the Xs with just the PMID number (no text).
  4. Under the reference write a short summary of the papers main findings.
    1. Only 1-2 paragraphs.
    2. Must not be a copy of the paper abstract.
  5. Save and you are done.

PubMed logo.gif

Lab 2 Assessment - Uploading an Image
  1. Upload a research image using the guide information below. The image uploaded for your individual assessment can relate to your project or from fertilisation to week 3 of development (upload only a single image).
  2. Add that image to your own individual page (see Images) including an image title and its reference link.
  3. No two students should upload the same image, check new images before you upload.
  4. No student can delete an image once uploaded, please contact me by email with the image address and I will delete (with no penalty, just glad to help out).


2016 Group Project Topic - Signaling in Development

OK you are now in a group

  1. Go to the blank group page and add a topic that interests you along with your student signature.
  2. No two groups can do the same topic, but at this stage the final topic has not yet been decided (next week).

Initially the topic can be as specific or as broad as you want.


Chicken embryo E-cad and P-cad gastrulation.png

Chicken embryo E-cad and P-cad gastrulation[1]

References

  1. <pubmed>27097030</pubmed>
Lab 4 Assessment - GIT Quiz

ANAT2341 Quiz Example | Category:Quiz | ANAT2341 Student 2015 Quiz Questions |

Design 4 quiz questions based upon gastrointestinal tract. Add the quiz to your own page under Lab 4 assessment and provide a sub-sub-heading on the topic of the quiz.

An example is shown below (open this page in view code or edit mode). Note that it is not just how you ask the question, but also how you explain the correct answer.

Lab 5 Assessment - Course Review
Complete the course review questionnaire and add the fact you have completed to your student page.
Lab 6 Assessment - Cleft Lip and Palate
  1. Identify a known genetic mutation that is associated with cleft lip or palate.
  2. Identify a recent research article on this gene.
  3. How does this mutation affect developmental signalling in normal development.
Lab 7 Assessment - Muscular Dystrophy
  1. What is/are the dystrophin mutation(s)?
  2. What is the function of dystrophin?
  3. What other tissues/organs are affected by this disorder?
  4. What therapies exist for DMD?
  5. What animal models are available for muscular dystrophy?
Lab 8 Assessment - Quiz
A brief quiz was held in the practical class on urogenital development.
Lab 9 Assessment - Peer Assessment
  • This will form part of your individual assessment for the course.
  • Each student should now look at each of the other Group projects in the class.
  • Next prepare a critical assessment (should include both positive and negative issues) of each project using the project group assessment criteria.
  • This assessment should be pasted without signature on the top of the specific project's discussion page. (minimum length 3-5 paragraphs/project)
  • This critical assessment should also be pasted on your own student page.
  • Each student should therefore have 5 separate reports pasted on their own page for this assessment item.
  • Length, quality and accuracy of your reports will be part of the overall mark for this assessment.
    • there will be a greater loading on this than simple question assessments.
Lab 10 Assessment - Stem Cells
As part of the assessment for this course, you will give a 15 minutes journal club presentation in Lab 10. For this you will in your current student group discuss a recent (published after 2011) original research article (not a review!) on stem cell biology or technology.
Lab 10 - Stem Cell Presentations 2016
Group Mark Assessor General Comments

Group 1: 15/20

Group 2: 19/20

Group 3: 20/20

Group 4: 19/20

Group 5: 16/20

Group 6: 16/20

The students put great effort in their presentation and we heard a nice variety of studies in stem cell biology and regenerative medicine today. The interaction after the presentation was great.

As general feedback I would like to advise students to:

  • Never discuss M&M as a separate section in journal clubs. I gave this advice prior to the lab, but still most groups did talk through the M&M section.
  • Do not use your slides as cheat sheets, avoid text on slides, know what messages you need to get across, use images to illustrate these
  • Engage with your slides. Talk through them. Point at panels. Gauge your audience’s understanding by making eye contact with them
  • Avoid using abbreviations. Most people do not readily understand these and will lose track
Lab 11 Assessment - Heart Development
Read the following recent review article on heart repair and from the reference list identify a cited research article and write a brief summary of the paper's main findings. Then describe how the original research result was used in the review article.

<pubmed>26932668</pubmed>Development

ANAT2341Lectures - Textbook chapters  
Lecture (Timetable) Textbook - The Developing Human Textbook - Larsen's Human Embryology
Embryology Introduction Introduction to the Developing Human
Fertilization First Week of Human Development Gametogenesis, Fertilization, and First Week
Week 1 and 2 Second Week of Human Development Second Week: Becoming Bilaminar and Fully Implanting
Week 3 Third Week of Human Development Third Week: Becoming Trilaminar and Establishing Body Axes
Mesoderm Fourth to Eighth Weeks of Human Development Fourth Week: Forming the Embryo
Ectoderm Nervous System Development of the Central Nervous System
Early Vascular Cardiovascular System Development of the Vasculature
Placenta Placenta and Fetal Membranes Development of the Vasculature
Endoderm - GIT Alimentary System Development of the Gastrointestinal Tract
Respiratory Respiratory System Development of the Respiratory System and Body Cavities
Head Pharyngeal Apparatus, Face, and Neck Development of the Pharyngeal Apparatus and Face
Neural Crest Nervous System Development of the Peripheral Nervous System
Musculoskeletal Muscular System Development of the Musculoskeletal System
Limb Development of Limbs Development of the Limbs
Renal Urogenital System Development of the Urinary System
Genital Urogenital System Development of the Urinary System
Stem Cells
Integumentary Integumentary System Development of the Skin and Its Derivatives
Endocrine Covered through various chapters (see also alternate text), read head and neck, neural crest and renal chapters.
Endocrinology Textbook - Chapter Titles  
Nussey S. and Whitehead S. Endocrinology: An Integrated Approach (2001) Oxford: BIOS Scientific Publishers; ISBN-10: 1-85996-252-1.

Full Table of Contents

Heart Cardiovascular System Development of the Heart
Sensory Development of Eyes and Ears Development of the Eyes
Fetal Fetal Period Fetal Development and the Fetus as Patient
Birth and Revision
Additional Textbook Content - The following concepts also form part of the theory material covered throughout the course.
  1. Principles and Mechanisms of Morphogenesis and Dysmorphogenesis
  2. Common Signaling Pathways Used During Development
  3. Human Birth Defect
ANAT2341 Course Timetable  
Week (Mon) Lecture 1 (Mon 1-2pm) Lecture 2 (Tue 3-4pm) Practical (Fri 1-3pm)
Week 2 (1 Aug) Introduction Fertilization Lab 1
Week 3 (8 Aug) Week 1 and 2 Week 3 Lab 2
Week 4 (15 Aug) Mesoderm Ectoderm Lab 3
Week 5 (22 Aug) Early Vascular Placenta Lab 4
Week 6 (29 Aug) Gastrointestinal Respiratory Lab 5
Week 7 (5 Sep) Head Neural Crest Lab 6
Week 8 (12 Sep) Musculoskeletal Limb Development Lab 7
Week 9 (19 Sep) Renal Genital Lab 8
Mid-semester break
Week 10 (3 Oct) Public Holiday Stem Cells Lab 9
Week 11 (10 Oct) Integumentary Endocrine Lab 10
Week 12 (17 Oct) Heart Sensory Lab 11
Week 13 (24 Oct) Fetal Birth and Revision Lab 12

ANAT2341 2016: Moodle page | ECHO360 | Textbooks | Students 2016 | Projects 2016

ANAT2341 Lab 1 Lab 1


Referencing

Fertilization

PMID 27486480

http://www.ncbi.nlm.nih.gov/pubmed/27486480

Lab 1 Assessment

<pubmed>27498612 </pubmed>

This research article aimed to study the effects of elevated progesterone levels on the trigger day of in-vitro fertilization and intracytoplasmic sperm injection (ICSI) cycles. 4106 IVF cycles were analysed, with the primary measure being live birth rate. The effects of increased progesterone that were studied include oocyte retrieval rates, fertilization and implantation rates as well as clinical pregnancy rates (measured when a heartbeat is detected) and live birth rates. All these outcomes were analysed among both groups of frozen embryo transfer and fresh transfer cycles. The negative impacts on oocyte and embryo quality found in previous studies, as well as potential negative impacts on fertilization and pregnancy rates were also closely observed. Progesterone levels only slightly increased on the trigger days of these cycles, but these increases were not found to be significant enough to be considered as more than a phenomenon. The implantation, clinical pregnancy rates and live birth rates were similar among the groups of fresh and frozen embryo transfer cycles, thus revealing that raised progesterone levels on the trigger day of these cycles had no impact on embryo quality and implantation, pregnancy and live birth rates, and no negative effects were detected.

Mark Hill 18 August 2016 - You have added the citation correctly and written a reasonable summary of the article findings. Your summary should have included an explanation/description for what the 2 key components of this study, Medroxyprogesterone acetate (MPA) and Human Menotrophins Gonadotrophin (hMG ). Assessment 4/5


Lab 2 Assessment

Cell number distribution for human embryos on day 3.png

This image is from an article titled "The Relationship between Cell Number, Division Behavior and Developmental Potential of Cleavage Stage Human Embryos: A Time-Lapse Study", found through the Public Library of Science. The graph conveys the results of one part of the study, that is, the cell number distribution for all embryos in this study on day 3. All embryos studied were human.


Mark Hill 29 August 2016 - Copyright and Student Image template correctly included with the file. The reference was not formatted correctly with the file or one your page here.

This is the code you need with the file <pubmed>27077739</pubmed> to display the reference correctly.

This is the code you need or a citation as shown correctly on your page here.<ref name="PMID27077739"><pubmed>27077739</pubmed></ref>

FYI - I have added the figure (resized) with a legend and the citation below.

Assessment 4/5

Cell number distribution for human embryos on day 3.png

Cell number distribution for human embryos on day 3[1]


Lab 3 Assessment

Mark Hill 31 August 2016 - Lab 3 Assessment Quiz - Mesoderm and Ectoderm development.

Question 1 - somites

Question 2 - paraxial

Question 3 - brain vesicles

Question 4 - brain flexures

Question 5 - maternal diet (half mark)

Assessment 5/5



References

  1. <pubmed>27077739</pubmed>

Lab 4 Assessment : Quiz

Quiz

1 Endoderm in the Gastrointestinal tract begins to proliferate in week 6.

true
false

2 How is the gastrointestinal tract divided?

aorta, celiac artery, superior mesentaric artery, inferior mesentaric artery
Foregut, midgut and hindgut
Ventral mesogastrium, duodenum, caecum
spleen, pancreus, mesentery

3 The spleen loses its blood cell forming abilities as the embryo continues to develop.

true
false

4 The pancreatic duct forms from the:

splanchnic mesoderm
dorsal and ventral mesentery
ventral bud duct and distal part of dorsal bud

5 The liver differentiates into the hepatic diverticulum and paracaval portion which then produces the gall bladder.

true
false


Lab 5 Assessment

Questionnaire completed


Lab 6 Assessment

The deletion or mutation of the epidermal growth factor receptor at the medial edge epithelium where the Transforming Growth Factor a is expressed is one of the identified genetic mutations that results in cleft palate.

A recent research article on this gene: Parental cigarette smoking, transforming growth factor-alpha gene variant and the risk of orofacial cleft in Iranian infants

How does this mutation affect developmental signalling in normal development: TGFA is concentrated in the epithelial tissue located in the medial edges of palatal shelves. In normal development, this gene assists in the production of extracellular matrix and also enables the palate to fuse without disruption by promoting normal movement and positioning of mesenchymal cells. Three mutations of TGFA associated with cleft palate are RsaI, and TaqI in intron 5 and BamHI in exon 6. When this gene is mutated, an abnormal or incomplete formation of the palate occurs.