User:Z5020317

From Embryology
Revision as of 13:04, 4 September 2015 by Z5020317 (talk | contribs)

--Mark Hill (talk) 10:47, 6 August 2015 (AEST) Thanks for setting up your page. We will be talking more about this in the Practical on Friday.

Lab 1 Assessment

Your Lab assessment now requires you to find a 2 recent research references on fertilisation or in vitro fertilisation.

Paste each reference on your page, as shown in the class.

Write below each reference a brief summary of the research article methods and findings.

The summary for each need not be more than 3-4 paragraphs in length.

This will need to be completed before next weeks laboratory.

The use of r-hFSH in treatment of idiopathic male factor infertility before ICSI.

look at this [1]

PMID 26166637

The objective of this investigations was to evaluate whether or not the pre-treatment use of Recombinant Human Follicle Stimulating Hormone (r-hFSH), could be used to treat common male infertility issues as Idiopathic Oligozoospermia, to improve their clinical results of Intracytoplasmic Sperm Injection (ICSI).

They selected a sample of 82 couples that were infertile due to the Idiopathic male factor, from the Biofertility Center in Rome, Italy between May 2013- April 2014 and randomly selected a treatment group of 36 males, Group A, and control group of 46 men, Group. The men in treatment Group A were subcutaneously injected with 150 IU of R-hFSH; Gonad F© (recombinant Human FSH) 3 times per week, for a 3 month period. The subjects in the control Group B were injected with a placebo and therefore received no treatment. After the three month period all 82 couples underwent a cycle of ICSI. In each group fertilisation, rate of implantation, rate of clinical pregnancy and the rate of miscarriage were assessed.

They found that the rate fertilisation was comparable in both treatment groups, although treatment group A had a significantly higher implantation rate and clinical pregnancy rate as well as a statistically significant lower rate of miscarriage (P<0.05). The conclusion that they drew was that the treatment use of r-hFSH to treat Idiopathic males infertility prior to ICSI can improve the clinical rate of pregnancy, and increase the rate of implantation as well as decrease the chances of early pregnancy loss.


Effect of hepatitis C virus infection on the outcomes of in vitro fertilization.

look at this [2]

PMID 26131230

The objective of this experiment was to investigate the effects that the Hepatitis C Virus (HCV) infection has upon the outcome In Vitro Fertilisation (IVF). From January 2008 - December 2013 a large sample of data was collected from couples opting for IVF at the Centre for Reproductive Medicine, General Hospital of Tianjin Medical University. These 1424 couples were tested for the HCV antibody (HCV-Ab) and were then separated into 3 groups. Group A contained 90 couples where the female tested HCV positive, Group B had 78 couples in which the male partner tested HCV positive and Group C - was the control group, of 1256 couples that both tested negative for HCV. The control group was also matched in age and in accordance with the IVF ovarian hyper stimulation protocol. These couples then underwent IVF and the results were closely monitored.

Initially the sperm was collected and its concentration, volume, motility, normal sperm morphology percentage (NMP) and Teratozoospermia index (TZI) were assessed, and again tested for the presence of HCV-Ab. The women were tested with Follicle Stimulating Hormone (FSH) on days 2-4 of their Menstrual cycle, within the 6 months of IVF treatment. They underwent Ovarian Hyperstilumation, and intramuscular injections of chronic Gonadotropin to mature the Oocytes. Transvaginal collection of mature Oocytes occurred roughly 36 hours later. 3 days later 2 high quality embryos were then transferred back into the uterus and oral progesterone was administered to support the Luteal phase of menstruation cycle. 14 days after the embryo transfer, pregnancy can be detected by testing for increasing levels of hCG in the blood, and can later, be clinically diagnosed through the presence of gastrointestinal sac during an abdominal ultrasound.

They found that all the groups (HVC positive male, HCV positive female , HCV negative couples) had similar results in terms of age, ovarian response, endometrial thickness, duration of Gonadotropin administration, embryo transfer and day of hCG administration, and no obvious differences within the sperm parameters; motility, concentration , PR differences, volume and NMP, TZI. They proceeded to investigate the effects of HCV on pregnancy and found no notable differences on pregnancy rates per cycle. They came to the conclusion that HVC had no effect upon IVF treatment or pregnancy.

--Mark Hill (talk) 10:36, 4 September 2015 (AEST)These are good summaries of the 2 papers. You did not need to keep "look at this " at the front of the reference code. (5/5)

Lab 2 Assessment

Upload a research image using the guide information below. The image uploaded for your individual assessment can relate to your project or from fertilisation to week 3 of development (upload only a single image).

Add that image to your own individual page (see Images) including an image title and its reference link.

Uploading Images in 5 Easy Steps  
First Read the help page Images and Copyright Tutorial.
Hint - This exercise is best done by using separate tabs on your browser so that you can keep all the relevant pages easily available. You can also use your own discussion page to copy and paste links, text. PMIDs etc that you will need in this process.
  1. Find an image .
    1. Search PubMed using an appropriate search term. Note that there is a special library of complete (full online) article and review texts called PubMed Central (PMC). Be very careful, while some of these PMC papers allow reuse, not all do and to add the reference link to your image you will still need to use the PMID.
    2. You can also make your own search term. In this link example PMC is searched for images related to "embryo+implantation" http://www.ncbi.nlm.nih.gov/pmc/?term=embryo+implantation&report=imagesdocsum. simply replace "embryo+implantation" with your own search term, but remember not everything in PMC can be reused, you will still need to find the "copyright notice" on the full paper, no notice, no reuse.
    3. Where else can I look? BioMed Central is a separate online database of journals that allow reuse of article content. Also look at the local page Journals that provides additional resources.
    4. You have found an image, go to step 2.
  2. Check the Copyright. I cannot emphasise enough the importance of this second step.
    1. The rule is unless there is an obvious copyright statement that clearly allows reuse (there are several different kinds of copyright, some do not) located in the article or on the article page, move on and find another resource. Not complying with this is a serious academic infringement equivalent to plagiarism."Plagiarism at UNSW is defined as using the words or ideas of others and passing them off as your own." (extract from UNSW statement on Academic Honesty and Plagiarism)
    2. You have found the statement and it allows reuse, go to step 3.
  3. Downloading your image.
    1. Download the image to your own computer. Either use the download image on the page or right click the image.
    2. To find the downloaded image you may have to look in your computer downloads folder, or the default location for downloaded files.
    3. The image file will have its own original name, that you will not be using on the wiki. You can rename it now (see renaming below), but you should also make a note of the original name.
    4. Make sure you have everything ready then for the
    5. You have the image file on your computer, go to step 4.
  4. Uploading your image.
    1. First make sure you have all the information you want to use with the file readily available. There is also a detailed description below.
    2. Towards the bottom of the lefthand menuunder “Toolbox” click Upload file. This will open a new window.
    3. In the top window "Source file", click "Choose file" and then navigate to find the file on the computer. and select the image.
    4. If you have done this correctly the upload window will now have your image file shown in choose file and also in the lower window "File description" in "Destination filename:" DO NOT CLICK UPLOAD FILE YET.
    5. Rename your file in "Destination filename:" this should be a brief filename that describes the image. Not any of the following - the original file name, image, file, my image, your ZID, etc. Many of the common embryology names may have already been used, but you can add a number (01, 02, 03, etc) or the PMID number to the filename to make it unique.
    6. If the filename or image has already been used or exists it will be shown on the upload page. If another student has already uploaded that image you will have to find another file. Duplicated images will not receive a mark, so check before you upload as you cannot delete images.
    7. In the "Summary" window for now just paste the PMID. You will come back and edit this information.
    8. Now click "Upload image" at the bottom of the window, go to step 4.
  5. Edit and Add to your page.
    1. Edit - Open the image with the "Edit" tab at the top of its page. You should see the PMID you had pasted earlier in the new edit window. Add the following information to the summary box.
      1. Image Title as a sub-heading. Under this title add the original figure legend or your own description of the image.
      2. Image Reference sub-sub-heading. Use the PMID link method shown in Lab 1 and you can also have a direct link to the original Journal article.
      3. Image Copyright sub-sub-heading. Add the copyright information under this sub-sub-heading exactly as shown in the original paper.
      4. Student Image template, as shown here {{Template:Student Image}} to show that it is a student uploaded image.
    2. Add - Now add your image to your own page under a subheading for Lab 2 Assessment including a description and a reference link. If still stuck with this last step, look at the example on the Test Student page.
    3. Done!

Students cannot delete images once uploaded. You will need to email me with the full image name and request deletion, that I am happy to do with no penalty if done before I assess.

Non-Table version of this page

Stress Relief....

<html5media height="480" width="640">http://www.youtube.com/watch?v=i9Hwn2DOgKo</html5media>

look at this [3]

PMID 26250560



Ovarian Structural scematic and stained cortical region of an ovariana stem cell .jpg

Ovarian stem Cell Schematic [4]

thumb

PMID 26250560


--Mark Hill (talk) 10:42, 4 September 2015 (AEST) Image uploaded correctly with reference, copyright and student template. The uploaded image size is so small the text cannot be read, you should have used a larger version of the original figure. Just a few minor issues. The Image title (Ovarian Structural scematic and stained cortical region of an ovariana stem cell .jpg) is far too long and includes an unnecessary space at the end. This could have been (Ovarian stem cell cartoon.jpg). Your subheadings have not been updated/formatted correctly and you have not formatted the reference correctly (I have fixed the reference this please see the difference). While all these are minor, it shows you are not reviewing your work. (4/5)


Lab 3 Assessment

In Lab 3 you were asked to divide the group project among your members and to research your allocated project component.

You will need to demonstrate that you have actively researched your project topic over the last week by having your section heading appear on the project page and have at least 3 research/review articles listed under that sub-heading.

The same content should be passed on your own individual assessment page.


Prenatal Diagnosis

Prenatal diagnosis is the screening process that tests an early fetus for overall growth, complications of pregnancy, birth defects and chromosomal or genetic abnormalities within the first 2 trimesters. It aims to provide the parents with as information as possible to help them make an informed decision about the infants quality of life. In 90-95% of the cases negative outcomes occur; confirming the healthy state of the fetus, should a genetic abnormality be present, it provides the parents with the opportunity to investigate further with other tests, and possible fetal therapeutic treatments available as well plan and prepare for the disabled infant or to terminate the pregnancy.

Post Implantation Genetic Diagnosis

  • Indicators for prenatal screening/ high risk factors include:
    • maternal ages > 35 years
    • paternal ages > 50-55
    • history of 2+ miscarriages
    • previous pregnancy or family history of a preexisting genetic or chromosomal disorder
    • suspected carriers of genetic disorders
    • maternal disease/condition present (high BP, diabetes)
    • abnormal ultrasound or serum test results within the first 2 trimesters
    • family history of neural tube or other birth defects


  • Ultrasonography:
    • high frequency sound waves are used to generate a image of the fetus
    • relatively non-invasion; its conducted transabdominally or transvaginally ( producing a higher resolution image)
    • It reveals the presence/absence of congenital abnormalities, characteristics of fetal growth and development, uterine development status; amount of **amniotic fluid, placental position, umbilical blood flow and the presence of multiple gestation.
    • (if abnormalities are detected further testing is recommended)


  • Amniotic Fluid Analysis/:
    • samples are obtained through amniocentesis
    • the amniotic fluid is analyzed for its biochemical composition
    • earlier in the pregnancy it can be examined for sex determination and to diagnose genetic or chromosomal disorders present.
    • Later into the pregnancy it provides an indication of fetal maturity and well being
    • Feta; cells recovered from the amniotic fluid can be cultured for specific karyotypes, to test for Chromosomal Abnormalities, and analysed for Alpha- fetoprotein (AFP) a biochemical marker of metabolic disorders and neural tube defects as well as other abnormalities.
    • Amniocentesis- occurs usually between weeks 14-20 as amniotic fluid has reached the optimal volume (150-250mls) allowing 20-30mls to be removed with a relatively low risk or fetal or maternal complication, and in time for a 2nd trimester abortion.
    • early amniocentesis ( before week 13) increase the risk of fetal loss, leakage of essential amniotic fluid and talipes equinovarus.


  • Chronic Villus Sampling (CVS):
    • occurs roughly 10-13 weeks after last menstrual cycle, ensuing a sufficiently developed chorionic villi but before the chorion laeve forms the definitive placenta.
    • ultrasounds is used to locate the gestational sac and implantation then a transcervial or transabdominal approach is used to aspirate living tropoblast tissue.
    • sample is analyzed for chromosomal abnormalities or with enzyme assay.
    • advantages: earlier diagnosis , decreased waiting period
    • disadvantages: risk of spontaneous abortion, bacterial infection, bleeding, leakage of amniotic fluid, inability to diagnose neural tube defects this early, early cleavage (before wk 10) is associated with increased risk of limb defects (due to insufficiently developed chronic villi)


  • Umbilical Blood Sampling:
    • can occur as early as 16 weeks
    • using the umbilical cord to obtain fetal blood samples - with real time ultrasound
    • used to diagnose inherited blood disorders, to detect congenital infections, to assess fetal anemia and in treatments such as blood transfusions.
    • disadvantages: risks of infection, preterm labor, thrombosis, bleeding & transient fetal arrhythmia


  • Fluroscent in Situ Hybridisation (FISH)
    • rapidly detects (within 24 hours of testing ) the presence of Trisomies 21, 13 and 8 and alterations in sex chromosomes in uncultured cells.


fetal therapies

early diagnosis allows the opportunity for intervention:

  • surgical intervention urinary tract obstruction ; aiming to reduce prenatal renal damage
  • fetal transfusions ( feta anemia
  • fetal medical treatmetn ( fetal cardiac arrhythmias,impaired thyroid function etc.) treatment occurs usually through the mother
  • infusions for hematologic conditions
  • stem cell transplantation
  • gene therapy
  • pharmocolic interventions


textbooks used :

  • Maternal, Fetal & Neonatal physiology: a Clinical perspective [5]
  • Langman's Medical Embryology (12th ed.)Chapter 9, pages 125-129 [6]

the following are two articles about a newly available and accessible prenatal non- invasive genetic test- Blood sampling:

  • Report on Cutting edge prenatal screening technology to become available in Australia [7]
  • Blood Test takes risk out of prenatal testing [8]
  • Prenatal screening and Diagnostic Tests Information Pamphlet [9]

<pubmed>26168107</pubmed>



--Mark Hill (talk) 10:46, 4 September 2015 (AEST) While you have included a lot of content for your group project, I only see a single PubMed reference here? The other sources are useful, but begin with the research literature. (4/5)

Lab 4 Assessment

ANAT2341 Quiz Example | Category:Quiz | ANAT2341 Student 2015 Quiz Questions |

Design 3 quiz questions based upon any of the major subjects we have covered to date. Add the quiz to your own page under Lab 4 assessment and provide a sub-sub-heading on the topic of the quiz. An example is shown below (open this page in view code or edit mode).

Gamatogenesis

1 { Which is the most correct statement about male Gamatogenesis :

the entire proces is driven by the secretions of the sertoli cells
it does not occur before puberty
meiosis is initiated by meitoically dividing stem cells
it takes a males spermatozoa 38 hours from commencing meiosis until it reaches a mature spermtozoa.
sperm does does not reach full maturity until fertilization.

2 The most correct sequence of spermatogenesis ( male gamatogenesis ) is:

spermatozoa, spermatodytes, spermatid, spermatagonia
sertoli cells, spermatocytes, spermatagonia, spermatozoa
spermatogonia, spermatocytes, spermatids, spermatozoa
spermatocytes, spermatids, spermatogonia, spermatozoa

3 Which of the following statements about gamatogenesis are True:

  • A) Oogenesis enter arrest at the 1st meiotic stage and spermatogenesis does not enter arrest
  • B) Meiosis is initiated twice in a finite population of cells
  • C) Spermatogenesis produces 4 gametes/meiosis whereas oogenesis produces 1 gamete/meiosis
  • D) In Oogenesis gamate differentiation occurs at the diploid phase and spermatogenesis it occurs in the haploid phase.

none of the above
A and C
A,B,D
A,C,D
all of the above


- neither gamate has reached its full maturity when it leaves the gonad - spermatogenesis can not occur untill puberty and oogenesis is reactivated at puberty due to hormual stimulation from the endocrine system


--Mark Hill (talk) 10:51, 4 September 2015 (AEST) These are indeed some thought provoking questions you have devised here. You could also have included links to pages where the student could read more about the topics. (9/10)

ANAT2341 Student 2015 Quiz Questions

Lab Attendance

--Z5020317 (talk) 13:46, 7 August 2015 (AEST)

--Z5020317 (talk) 13:15, 14 August 2015 (AEST)

Test student 2015

References

  1. <pubmed>26166637</pubmed>
  2. <pubmed>26131230</pubmed>
  3. <pubmed>26250560</pubmed>
  4. <pubmed>26250560</pubmed>| [1]
  5. Blackburn, S.L. (2003) Maternal, Fetal & Neonatal physiology: a Clinical perspective (2nd ed.). Seattle: Saudners
  6. Sadler T.W.(2012) Langman's Medical Embryology (12th ed.) Philadelphia: Lipincott, Wiliams & Wilkins, a Wolters Kluwer Business
  7. Carbonell, R. Shinners, A. Amor, D. Mark, D. (2015) Report on Cutting edge prenatal screening technology to become available in Australia: Prepared for ABC news, PM with Mark Colvin. Retrieved from {http://www.abc.net.au/pm/content/2015/s4203200.htm}
  8. Begley, S. (05/07/2015) Blood Test takes risk out of prenatal testing. ABC Science. Retrieved from {http://www.abc.net.au/science/articles/2012/07/05/3539549.htm}
  9. Western Australia. Department of Health Genetics Council Prenatal Diagnosis Committee (2011)Prenatal screening and Diagnostic Tests. Retrieved from {http://www.health.wa.gov.au/docreg/Education/Prevention/Genetics/HP3131_prenatal.pdf}



Please do not use your real name on this website, use only your student number.

2015 Course: Week 2 Lecture 1 Lecture 2 Lab 1 | Week 3 Lecture 3 Lecture 4 Lab 2 | Week 4 Lecture 5 Lecture 6 Lab 3 | Week 5 Lecture 7 Lecture 8 Lab 4 | Week 6 Lecture 9 Lecture 10 Lab 5 | Week 7 Lecture 11 Lecture 12 Lab 6 | Week 8 Lecture 13 Lecture 14 Lab 7 | Week 9 Lecture 15 Lecture 16 Lab 8 | Week 10 Lecture 17 Lecture 18 Lab 9 | Week 11 Lecture 19 Lecture 20 Lab 10 | Week 12 Lecture 21 Lecture 22 Lab 11 | Week 13 Lecture 23 Lecture 24 Lab 12 | 2015 Projects: Three Person Embryos | Ovarian Hyper-stimulation Syndrome | Polycystic Ovarian Syndrome | Male Infertility | Oncofertility | Preimplantation Genetic Diagnosis | Students | Student Designed Quiz Questions | Moodle page

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