User:Z5017002: Difference between revisions
No edit summary |
|||
(37 intermediate revisions by 2 users not shown) | |||
Line 2: | Line 2: | ||
==Lab Attendance== | ==Lab Attendance== | ||
===Lab 1=== | |||
[[User:Z5017002|Z5017002]] ([[User talk:Z5017002|talk]]) 14:57, 5 August 2016 (AEST) | [[User:Z5017002|Z5017002]] ([[User talk:Z5017002|talk]]) 14:57, 5 August 2016 (AEST) | ||
== | ===Lab 2=== | ||
[[User:Z5017002|Z5017002]] ([[User talk:Z5017002|talk]]) 14:07, 12 August 2016 (AEST) | |||
===Lab 3=== | |||
[[User:Z5017002|Z5017002]] ([[User talk:Z5017002|talk]]) 14:01, 19 August 2016 (AEST) | |||
===Lab 4=== | |||
[[User:Z5017002|Z5017002]] ([[User talk:Z5017002|talk]]) 14:09, 26 August 2016 (AEST) | |||
===Lab 5=== | |||
absent due to illness | |||
===Lab 6=== | |||
[[User:Z5017002|Z5017002]] ([[User talk:Z5017002|talk]]) 14:12, 9 September 2016 (AEST) | |||
===Lab 7=== | |||
[[User:Z5017002|Z5017002]] ([[User talk:Z5017002|talk]]) 14:05, 16 September 2016 (AEST) | |||
===Lab 8=== | |||
===Lab 9=== | |||
===Lab 10=== | |||
===Lab 11=== | |||
===Lab 12=== | |||
[[User:Z5017002|Z5017002]] ([[User talk:Z5017002|talk]]) 14:02, 21 October 2016 (AEDT) | |||
{| width=95% | |||
|-bgcolor="FAF5FF" | |||
| [mailto:m.hill@unsw.edu.au Mark Hill] 17 October 2016 - There are a number of individual assessment items that have not been completed here. | |||
|} | |||
==Useful Links== | |||
===External Link=== | ===External Link=== | ||
Line 16: | Line 59: | ||
[[Student Page]] | [[Student Page]] | ||
==Individual Assessment== | |||
Type in a group? A mix of everything: co-ordinator, team worker, resource-investigator, implementer, shaper, monitor-evaluator! | |||
The most interesting thing I learnt in the fertilisation lecture? That the oocyte doesn't complete meiosis II until it's fertilised! didn't know that before, wacky as. | |||
===Lab 1 Assessment=== | |||
Article: | |||
<pubmed>27309808</pubmed> | |||
In this article by Ohto et al. (2016), the group report on findings from their study into the crystal structures of two key proteins involved in sperm–oocyte fusion in human fertilization. IZUMO1 is a sperm transmembrane protein and JUNO is its corresponding receptor found on the surface of the oocyte. X-ray crystallography was used to elucidate the molecular mechanisms behind their interaction and formation of the IZUMO1–JUNO complex. The study found IZUMO1 to bear an elongated rod-shaped structure 90 Å in length, consisting of an N-terminal alpha-helical bundle IZUMO domain and a C-terminal immunoglobulin-like domain, connected by an intervening β-hairpin region via disulfide bonds. This central β-hairpin region was shown to provide the main platform for specific binding to the surface behind the ligand binding pocket on JUNO, which exhibits a single globular fold comprised of eight α-helices and four β-strands, stabilized by eight disulfide bonds. Hydrophobic and van der Waals interactions are the main contributing forces to the binding along with six hydrogen bonds. Subsequent cell–oocyte binding assays were successfully conducted using mouse cells expressing either wild-type or mutant Izumo1 proteins to confirm the functional relevance of the IZUMO1–JUNO interface in sperm–oocyte attachment. Overall the study provided a major development in determining the mechanisms of sperm-oocyte recognition and the IZUMO1–JUNO as an essential part of fertilization. The findings have potential applications in contributing to the development of new therapeutics relating to fertility, such as contraceptive agents. | |||
{| width=95% | |||
|-bgcolor="FAF5FF" | |||
| [mailto:m.hill@unsw.edu.au Mark Hill] September 2016 - This is a relevant article and summary for fertilisation topic. Added after the submission date. | |||
| Assessment 4/5 | |||
|} | |||
===Lab 2 Assessment=== | |||
[[File:Primite Streak Cell Migration In Chick Embryo.png]] | |||
Primitive Streak Cell Migration In Chick Embryo | |||
<ref><pubmed>20485500</pubmed></ref> | |||
{| width=95% | |||
|-bgcolor="FAF5FF" | |||
| [mailto:m.hill@unsw.edu.au Mark Hill] September 2016 - Image added with correct reference, copyright and student template. | |||
| Assessment 5/5 | |||
|} | |||
===Lab 3 Assessment=== | |||
{| width=95% | |||
|-bgcolor="FAF5FF" | |||
| [mailto:m.hill@unsw.edu.au Mark Hill] 31 August 2016 - Lab 3 Assessment Quiz - [[Lecture_-_Mesoderm_Development|Mesoderm]] and [[Lecture_-_Ectoderm_Development|Ectoderm]] development. | |||
| Assessment 5/5 | |||
|} | |||
===Lab 4 Assessment=== | |||
Gastrointestinal Tract Quiz. | |||
<quiz display=simple> | |||
{'''Match''' the germ layer with the aspect of the gastrointestinal tract it will contribute to. | |||
* A) Endoderm | |||
* B) Mesoderm | |||
* C) Ectoderm | |||
* 1. enteric nervous system | |||
* 2. epithelium and associated glands, associated organs | |||
* 3. smooth muscle, mesentery, connective tissue, blood vessels, associated organs | |||
|type=”()”} | |||
<br> | |||
- A1, B2, C3 | |||
- A1, B3, C2 | |||
- A2, B1, C3 | |||
+ A2, B3, C1 | |||
- A3, B1, C2 | |||
- A3, B2, C1 | |||
|| Endoderm contributes to forming the epithelium and associated glands of the GIT and associated organs. The splanchnic part of the mesoderm also contributes to associated organs as well as the mesentery, connective tissues, smooth muscle and blood vessels of the GIT. The neural crest, part of the ectoderm, contributes to the formation of the enteric nervous system. Therefore “A2, B3, C1” is the correct answer. | |||
{Select the processes in GIT development which '''DO NOT''' occur in week 4: | |||
|type=”()”} | |||
- hepatic diverticulum development | |||
- differentiation of hepatic cells | |||
+ recanalization of the GIT endoderm tube | |||
- enlarged lumen of the GIT endoderm tube | |||
+ extension of the dorsal mesogastrium to form the greater omentum | |||
|| After becoming occluded in week 5, recanalization of the GIT endoderm tube occurs from weeks 6-8. Development of the mesentery occurs in Week 5. | |||
{The '''CORRECT''' adult structures which will go on to be formed from the Midgut division are: | |||
|type=”()”} | |||
- stomach, upper duodenum, lower duodenum, jejunum, ileum, cecum, appendix | |||
- lower duodenum, jejunum, ileum, cecum, appendix, liver, gallbladder, pancreas | |||
+ lower duodenum, jejunum, ileum, cecum, appendix, ascending colon, half transverse colon | |||
- jejunum, ileum, cecum, appendix, ascending colon, transverse colon, descending colon | |||
|| Supplied by the superior mesenteric artery, the midgut division in the adult is defined as the lower duodenum, jejunum, ileum, cecum, appendix, the ascending colon and the right half of the transverse colon. | |||
{Which of the following statements is/are '''CORRECT''' regarding abnormalities in GIT development: | |||
|type=”()”} | |||
+ Atresia is an interruption of the lumen whilst stenosis is a narrowing of the lumen | |||
+ Meckel’s Diverticulum results from improper closure and absorption of the vitelline duct which connects the yolk to the primitive GIT during early development | |||
- Meckel’s Diverticulum is rare, occurring in 1/100,000 births. | |||
+ Intestinal Malrotation usually presents in neonates and infants with bilious vomiting, bloody stools, recurrent abdominal pain, intestinal obstruction, abdominal distention, malabsorption/diarrhea, peritonitis and bile duct obstruction. | |||
- Intestinal Aganglionosis is a congenital abdominal wall defect which results in herniation of fetal abdominal viscera into the amniotic cavity. | |||
|| Meckel’s Diverticulum is common, occurring in 1-2% of the general population, therefore the third statement is not correct. Gastroschisis, not intestinal aganglionosis, results in herniation of fetal abdominal viscera into the amniotic cavity. Intestinal Aganglionosis is actually caused by the lack of the neural ganglia of the enteric nervous system in the intestine, which inhibits gastric motility via peristalsis. | |||
</quiz> | |||
===Lab 5 Assessment=== | |||
Course Feedback Questionnaire - Absent due to illness therefore could not complete in-class questionnaire | |||
===Lab 6 Assessment=== | |||
'''Identify a known genetic mutation that is associated with cleft lip or palate:''' | |||
Mutation in X-linked TBX22 gene (T-box 22). | |||
'''Identify a recent research article on this gene:''' | |||
<ref><pubmed>25373698</pubmed></ref> | |||
'''How does this mutation affect developmental signaling in normal development:''' | |||
===Lab 7 Assessment=== | |||
Duchenne Muscular Dystrophy | |||
*Populations Affected: | |||
** | |||
*Current and Emerging Therapeutics: | |||
**Corticoteroids | |||
*Aetiology: | |||
**mutations in the protein Dystrophin | |||
**only apparent postnatally once muscles in use | |||
*Tissues Affected: | |||
**skeletal muscle | |||
*Diagnosis | |||
**prenatal genetic diagnosis | |||
**muscle biopsy | |||
*Animal Models for DMD: | |||
**MDX Mouse Model | |||
{| width=95% | |||
|-bgcolor="FAF5FF" | |||
| [mailto:m.hill@unsw.edu.au Mark Hill] 11 October 2016 - You have answered most of the questions, some of which are too general, and do not included any citations for your answers. | |||
| Assessment 3/5 | |||
|} | |||
===Lab 8 Assessment=== | |||
{| width=95% | |||
|-bgcolor="FAF5FF" | |||
| [mailto:m.hill@unsw.edu.au Mark Hill] 27 October 2016 | |||
| Assessment 4.5/8 | |||
|} | |||
===Lab 9 Assessment=== | |||
Peer Assessment of Group Presentations: Assessment not completed | |||
You could have added some peer assessment here, even if submitted late. 0/10 | |||
===Lab 10 Assessment=== | |||
{{Stem Cell Presentations 2016}} | |||
===Lab 11 Assessment=== | |||
0/5 | |||
'''Cited research article on heart repair from review by Foglia & Poss (2016):''' | |||
'''Summary of findings:''' | |||
'''How the original research result was used in the Foglia & Poss review:''' | |||
==References== | |||
<references/> |
Latest revision as of 16:20, 17 November 2016
Student Information (expand to read) | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Individual Assessments | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Please leave this template on top of your student page as I will add your assessment items here. Beginning your online work - Working Online in this course
Click here to email Dr Mark Hill | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Lab 1 Assessment - Researching a Topic | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
In the lab I showed you how to find the PubMed reference database and search it using a topic word. Lab 1 assessment will be for you to use this to find a research reference on "fertilization" and write a brief summary of the main finding of the paper.
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Lab 2 Assessment - Uploading an Image | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
OK you are now in a group
Initially the topic can be as specific or as broad as you want. Chicken embryo E-cad and P-cad gastrulation[1] References
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Lab 4 Assessment - GIT Quiz | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
ANAT2341 Quiz Example | Category:Quiz | ANAT2341 Student 2015 Quiz Questions | Design 4 quiz questions based upon gastrointestinal tract. Add the quiz to your own page under Lab 4 assessment and provide a sub-sub-heading on the topic of the quiz. An example is shown below (open this page in view code or edit mode). Note that it is not just how you ask the question, but also how you explain the correct answer. | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Lab 5 Assessment - Course Review | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Complete the course review questionnaire and add the fact you have completed to your student page. | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Lab 6 Assessment - Cleft Lip and Palate | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Lab 7 Assessment - Muscular Dystrophy | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Lab 8 Assessment - Quiz | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
A brief quiz was held in the practical class on urogenital development. | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Lab 9 Assessment - Peer Assessment | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Lab 10 Assessment - Stem Cells | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
As part of the assessment for this course, you will give a 15 minutes journal club presentation in Lab 10. For this you will in your current student group discuss a recent (published after 2011) original research article (not a review!) on stem cell biology or technology.
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Lab 11 Assessment - Heart Development | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Read the following recent review article on heart repair and from the reference list identify a cited research article and write a brief summary of the paper's main findings. Then describe how the original research result was used in the review article.
<pubmed>26932668</pubmed>Development | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
|
Lab Attendance
Lab 1
Z5017002 (talk) 14:57, 5 August 2016 (AEST)
Lab 2
Z5017002 (talk) 14:07, 12 August 2016 (AEST)
Lab 3
Z5017002 (talk) 14:01, 19 August 2016 (AEST)
Lab 4
Z5017002 (talk) 14:09, 26 August 2016 (AEST)
Lab 5
absent due to illness
Lab 6
Z5017002 (talk) 14:12, 9 September 2016 (AEST)
Lab 7
Z5017002 (talk) 14:05, 16 September 2016 (AEST)
Lab 8
Lab 9
Lab 10
Lab 11
Lab 12
Z5017002 (talk) 14:02, 21 October 2016 (AEDT)
Mark Hill 17 October 2016 - There are a number of individual assessment items that have not been completed here. |
Useful Links
External Link
Internal Link
https://embryology.med.unsw.edu.au/embryology/index.php/2011_Lab_1
Individual Assessment
Type in a group? A mix of everything: co-ordinator, team worker, resource-investigator, implementer, shaper, monitor-evaluator!
The most interesting thing I learnt in the fertilisation lecture? That the oocyte doesn't complete meiosis II until it's fertilised! didn't know that before, wacky as.
Lab 1 Assessment
Article:
<pubmed>27309808</pubmed>
In this article by Ohto et al. (2016), the group report on findings from their study into the crystal structures of two key proteins involved in sperm–oocyte fusion in human fertilization. IZUMO1 is a sperm transmembrane protein and JUNO is its corresponding receptor found on the surface of the oocyte. X-ray crystallography was used to elucidate the molecular mechanisms behind their interaction and formation of the IZUMO1–JUNO complex. The study found IZUMO1 to bear an elongated rod-shaped structure 90 Å in length, consisting of an N-terminal alpha-helical bundle IZUMO domain and a C-terminal immunoglobulin-like domain, connected by an intervening β-hairpin region via disulfide bonds. This central β-hairpin region was shown to provide the main platform for specific binding to the surface behind the ligand binding pocket on JUNO, which exhibits a single globular fold comprised of eight α-helices and four β-strands, stabilized by eight disulfide bonds. Hydrophobic and van der Waals interactions are the main contributing forces to the binding along with six hydrogen bonds. Subsequent cell–oocyte binding assays were successfully conducted using mouse cells expressing either wild-type or mutant Izumo1 proteins to confirm the functional relevance of the IZUMO1–JUNO interface in sperm–oocyte attachment. Overall the study provided a major development in determining the mechanisms of sperm-oocyte recognition and the IZUMO1–JUNO as an essential part of fertilization. The findings have potential applications in contributing to the development of new therapeutics relating to fertility, such as contraceptive agents.
Mark Hill September 2016 - This is a relevant article and summary for fertilisation topic. Added after the submission date. | Assessment 4/5 |
Lab 2 Assessment
Primitive Streak Cell Migration In Chick Embryo [1]
Mark Hill September 2016 - Image added with correct reference, copyright and student template. | Assessment 5/5 |
Lab 3 Assessment
Mark Hill 31 August 2016 - Lab 3 Assessment Quiz - Mesoderm and Ectoderm development. | Assessment 5/5 |
Lab 4 Assessment
Gastrointestinal Tract Quiz.
Lab 5 Assessment
Course Feedback Questionnaire - Absent due to illness therefore could not complete in-class questionnaire
Lab 6 Assessment
Identify a known genetic mutation that is associated with cleft lip or palate: Mutation in X-linked TBX22 gene (T-box 22).
Identify a recent research article on this gene: [2]
How does this mutation affect developmental signaling in normal development:
Lab 7 Assessment
Duchenne Muscular Dystrophy
- Populations Affected:
- Current and Emerging Therapeutics:
- Corticoteroids
- Aetiology:
- mutations in the protein Dystrophin
- only apparent postnatally once muscles in use
- Tissues Affected:
- skeletal muscle
- Diagnosis
- prenatal genetic diagnosis
- muscle biopsy
- Animal Models for DMD:
- MDX Mouse Model
Mark Hill 11 October 2016 - You have answered most of the questions, some of which are too general, and do not included any citations for your answers. | Assessment 3/5 |
Lab 8 Assessment
Mark Hill 27 October 2016 | Assessment 4.5/8 |
Lab 9 Assessment
Peer Assessment of Group Presentations: Assessment not completed
You could have added some peer assessment here, even if submitted late. 0/10
Lab 10 Assessment
Lab 10 - Stem Cell Presentations 2016 | |
---|---|
Group Mark | Assessor General Comments |
Group 1: 15/20 Group 2: 19/20 Group 3: 20/20 Group 4: 19/20 Group 5: 16/20 Group 6: 16/20 |
The students put great effort in their presentation and we heard a nice variety of studies in stem cell biology and regenerative medicine today. The interaction after the presentation was great.
As general feedback I would like to advise students to:
|
Lab 11 Assessment
0/5
Cited research article on heart repair from review by Foglia & Poss (2016):
Summary of findings:
How the original research result was used in the Foglia & Poss review: