Welcome to the 2014 Embryology Course!
- Each week the individual assessment questions will be displayed in the practical class pages and also added here.
- Copy the assessment items to your own page and provide your answer.
- Note - Some guest assessments may require completion of a worksheet that will be handed in in class with your student name and ID.
|Individual Lab Assessment|
|Lab 12 - Stem Cell Presentation Assessment||More Info|
Lab Assessment 1
- Predictive factors in in vitro fertilization(IVF): a systematic review and meta-analysis
Article focused on acquiring data from previous case studies and research papers, collaborating data to determine the success rate of pregnancy prior In-vitro fertilization and certain factors which play a part in a successful pregnancy from the procedure.
The articles which were used in the data pooling were eligible to be used if they evaluated the association between one or more of the pre-identified factors (listed below) and its effect on pregnancy after the use of IVF in an unselected patient group and were not considered if they reported on a specific patient group within the sub fertile IVF population or if no association was drawn between eh predictive factors and pregnancy or could not be determined. From the data the following factors were carefully analysed to determine if they play a part in the success rate of pregnancy post IVF; Age, Type of Infertility, Duration of Infertility, Basal Follicle stimulating hormones, Oocytes present, Fertilization method and Embryo Quality
From the 14 studies that were used in the data pooling, it was found that ‘women aged 35 years or older had significantly lower pregnancy chances compared with women who were younger than 25’ (Loendersloot et al., 2010) as well as studies showing that women who were aged above 30, in comparison to those in their mid-twenties had a lower chance of pregnancy. Age was recognised as a ‘continuous variable’ throughout the studies which showed decreasing chances of a successful pregnancy as the patients age increases. Further studies showed the relationship between the amount of bFSH (Basal Follicle Stimulating Hormones) and pregnancy. In 2 case studies, it was revealed that pregnancy chances were higher post IVF in patients with a lower level of FSH in comparison to women with a higher level of FSH concentration. Oddity Rates confirmed that ‘increasing bFSH values were associated with lower pregnancy rates after IVF’ 3 of the studies determined the correlation of embryo quality to pregnancy after IVF. It also found that women with embryos with higher development stage and morphology scores had higher pregnancy chances compared to those which scored lower and also, embryos which showed delayed or appropriate development stages had lower pregnancy chances compared to women with advanced development stages.
Lab Assesment 2
Immunofluroescent labelling of embryo for immunoreactive 5 - mehtylocytosine at different cell stages