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From Embryology

Lab Attendance

Lab 1: --Z3333429 (talk) 12:52, 6 August 2014 (EST)

http://www.ncbi.nlm.nih.gov/pubmed

PubMed

Lab 2: --Z3333429 (talk) 11:03, 13 August 2014 (EST)

Lab 3: --Z3333429 (talk) 11:12, 20 August 2014 (EST)

Lab 1 Assessment

Article 1

Effect of vitamin D status on clinical pregnancy rates following in vitro fertilization[1]

Summary

Research was carried out to investigate the possible effect of vitamin D on human reproduction. The goal was to detect whether vitamin D levels Recent studies suggest that vitamin D may play a role in human reproduction. Our goal was to investigate whether there is a correlation between vitamin D levels and implantation and clinical pregnancy rates in infertile women following IVF.

Method

  • Participants in this investigation were 173 women undergoing IVF at Mount Sinai Hospital, Toronto, Ontatrio.
  • Serum 25(OH)D samples were collected within a week of oocyte retrieval from the patients.
  • The vitamin D levels of the participants were measured according to serum 25-hydroxy-vitamin D (25[OH]D) levels
  • Patients were classified in two categories according to serum levels of 25(OH)D: sufficient (≥ 75 nmol/L) or insufficient (< 75 nmol/L). Of the 173 patients, 54.9% presented with insufficient 25(OH)D levels and 45.1% has sufficient levels.
  • A comparison was made between patient demographics and IVF cycle parameters between sufficient and insufficient groups.
  • Clinical pregnancy, as identified by ultrasound following 4-5 weeks after embryo transfer; was the primary outcome measurement.

Findings

The research found that women who presented with sufficient 25(OH)D levels had significantly higher rates of clinical pregnancy per IVF cycle (52.5%) as compared to women with insufficient levels (34.7%). A higher rate of implantation was detected in the sufficient 25(OH)D group, however the results were not statistically significant. The research calls for further investigation the findings showed that vitamin D levels might be a predictor of clinical pregnancy and vitamin D supplementation could provide a simple and economical method of improving clinical pregnancy rates, not only in women undergoing IVF, but also across the board.

Reference

<pubmed>25077107</pubmed> Alcohol consumption and quality of embryos obtained in programmes of in vitro fertilization

Article 2

Alcohol consumption and quality of embryos obtained in programmes of in vitro fertilization[2]

Summary

Alcohol consumption has been identified as one of the main stimulants that negatively affect the reproductive systems of both sexes. An investigation was carried out to analyse the effect of alcohol consumption of female participants on the quality of embryos obtained through IVF programmes.

Method

  • The study covered 54 women who received treatment due to infertility.
  • Of the 54 women who participated, 42.59% consumed alcohol. Records were examined of the class of embryos (A, B and C) that each woman presented in during treatment.
  • The database and statistical analyses were performed using computer software STATISTICA 7.1.

Findings

A statistically significant correlation was found between the occurrences of class B embryo in patients who consumed more than 25 grams of ethyl alcohol daily (72.72%). Women who consumed alcohol sporadically or those who abstained entirely from alcohol presented with 44.44% and 30% rates of class B embryos respectively. It was concluded that alcohol consumption (over 25 grams of ethyl alcohol) increases the likelihood of developing pooper quality embryos. More research should be carried out to investigate this further and it was suggested that active campaigns should be established to inform women of the negative affects of alcohol consumption on embryonic development.

Reference

<pubmed>24959808</pubmed> Effect of vitamin D status on clinical pregnancy rates following in vitro fertilization


References

  1. <pubmed>25077107</pubmed>
  2. <pubmed>24959808</pubmed>

Lab 2 Assessment

Microscopic Field Image of GAD and GABA in Fetal Rat Lung Tissue. Location of glutamic acid decarboxylase (GAD) and γ-aminobutyric acid (GABA) in fetal lungs of rats: Detection of antigens was used to identify the location of GAD and GABA on fetal lung tissue sections.[1]

Reference

  1. Chintagari NR, Jin N, Gao L, Wang Y, Xi D, et al. (2010) Role of GABA Receptors in Fetal Lung Development in Rats. PLoS ONE 5(11): e14171. doi:10.1371/journal.pone.0014171


Lab 3 Assessment

Down-regulation of sonic hedgehog expression in pulmonary hypoplasia is associated with congenital diaphragmatic hernia.

Surfactant Metabolism Dysfunction and Childhood Interstitial Lung Disease (chILD).

Evaluation of fetal vocal cords to select candidates for successful fetoscopic treatment of congenital high airway obstruction syndrome: preliminary case series.

Bronchopulmonary dysplasia

The epidemiology of meconium aspiration syndrome: incidence, risk factors, therapies, and outcome.

Outcomes of congenital diaphragmatic hernia: a population-based study in Western Australia.

Congenital diaphragmatic hernia.

Antenatal infection/inflammation and postnatal lung maturation and injury.

Laryngo-tracheo-oesophageal clefts.

The frequency, clinical significance, and pathological features of chronic chorioamnionitis: a lesion associated with spontaneous preterm birth.

Computer simulation analysis of normal and abnormal development of the mammalian diaphragm.