User:Z3333038: Difference between revisions

From Embryology
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* Techniques:
* Techniques:


# Amniocentesis <ref>Moore, K. L., Persaud, T. V. N. & Torchia, M. G.  (2013). ''The Developing Human'' (9th ed.). Philadelphia, PA: Elsevier Saunders. </ref>: Amniocentesis refers to sampling of the amniotic fluid by inserting a needle through the mother's anterior abdominal and uterine walls into the amniotic cavity by piercing the chorion and amnion. This technique is performed at 15 and 18 weeks gestation.
**Amniocentesis <ref>Moore, K. L., Persaud, T. V. N. & Torchia, M. G.  (2013). ''The Developing Human'' (9th ed.). Philadelphia, PA: Elsevier Saunders. </ref>: Amniocentesis refers to sampling of the amniotic fluid by inserting a needle through the mother's anterior abdominal and uterine walls into the amniotic cavity by piercing the chorion and amnion. This technique is performed at 15 and 18 weeks gestation.


## Chorionic Villus Sampling <ref>Moore, K. L., Persaud, T. V. N. & Torchia, M. G.  (2013). ''The Developing Human'' (9th ed.). Philadelphia, PA: Elsevier Saunders. </ref>: Biopsies of trophoblastic tissue are obtained by inserting a needle through the mother's abdominal wall and uterine walls to the uterine cavity. Sampling can also be performed through the cervix with a polyethylene catheter, guided by real-time ultrasonography. CVS can be performed sooner than amniocentesis at 10 and 12 weeks of gestation. However, the rate of miscarriage from CVS is higher than amniocentesis.  
**Chorionic Villus Sampling <ref>Moore, K. L., Persaud, T. V. N. & Torchia, M. G.  (2013). ''The Developing Human'' (9th ed.). Philadelphia, PA: Elsevier Saunders. </ref>: Biopsies of trophoblastic tissue are obtained by inserting a needle through the mother's abdominal wall and uterine walls to the uterine cavity. Sampling can also be performed through the cervix with a polyethylene catheter, guided by real-time ultrasonography. CVS can be performed sooner than amniocentesis at 10 and 12 weeks of gestation. However, the rate of miscarriage from CVS is higher than amniocentesis.  


* Abnormalities which can be identified:
* Abnormalities which can be identified:


# Down Syndrome (Trisomy 21): Amniocentesis and CVS allow for chromosome abnormality detection. <ref>Moore, K. L., Persaud, T. V. N. & Torchia, M. G.  (2013). ''The Developing Human'' (9th ed.). Philadelphia, PA: Elsevier Saunders. </ref>
# Down Syndrome (Trisomy 21): Amniocentesis and CVS allow for chromosome abnormality detection. <ref>Moore, K. L., Persaud, T. V. N. & Torchia, M. G.  (2013). ''The Developing Human'' (9th ed.). Philadelphia, PA: Elsevier Saunders. </ref>
 
# Tay-Sachs <ref>Sheth, K. (2012). ''Tay-Sachs Disease''. Retrieved from http://www.nlm.nih.gov/medlineplus/ency/article/001417.htm. </ref>
## Tay-Sachs <ref>Sheth, K. (2012). ''Tay-Sachs Disease''. Retrieved from http://www.nlm.nih.gov/medlineplus/ency/article/001417.htm. </ref>





Revision as of 17:24, 20 August 2012

Individual Assessments

Lab 1 Assessment

1. Identify the origin of In Vitro Fertilization and the 2010 nobel prize winner associated with this technique and add a correctly formatted link to the Nobel page.

The History of In Vitro Fertilisation

In vitro fertilisation (IVF) refers to the process of artificial fertilisation conducted ex vivo. The IVF technique was first described for non-human use. The earliest known research conducted was by Walter Heape from Cambridge University in the 1890s who reported the first known case of embryo transplantation in rabbits. In 1959, Dr. Min Chueh Chang published his work in Nature describing the first successful mammalian live birth (rabbits) after IVF therapy.

Eventually, the use of IVF for humans became a possibility and then a reality: in 1978, the first successful birth from IVF occurred in England. The success of this IVF birth is credited to Patrick Steptoe and Robert Edwards. In 2010, Edwards was awarded the Nobel Prize in Medicine for the development of human IVF therapy. Because of IVF, many couples have been given a chance to conceive. However, the history of IVF is still in the making with constant improvements in the technology being developed and applied.


2. Identify and add a PubMed reference link to a recent paper on fertilisation and describe its key findings (1-2 paragraphs).

Research in Fertilisation

In order for fusion between mammalian gametes to occur, a spermatozoon must first pass through the external layers surrounding the oocyte: the cumulus oophorus and the zona pellucida (ZP). It is believe that the acromosome reaction (AR) of the spermatozoa starts upon contact with the zona pellucida. Consequently, the cumulus cell layer is typically removed in studies of mouse sperm-oocyte interactions in order to facilitate fertilisation. The recent experiments of Jin et al. [1] sought to answer the question: "Where does the fertilising mouse spermatozoon begin the AR - in the cumulus [of the oocyte] or the zona pellucida?" Jin et al. [1] utilised fluorescence microscopy and transgenic mouse spermatozoa to conduct their investigation. Additionally, Jin et al. [1] used cumulus-free oocytes and cumulus-enclosed oocytes to study the role of the cumulus cells in fertilisation.

From the experiment, Jin et al. [1] found that most fertilising spermatozoa begin the AR before their first contact with the ZP. The significance of this finding was that the spermatozoa with intact acromosomes at the ZP seldom had the ability to penetrate through [1]. In contrast, spermatozoa which had already began the AR could easily penetrate the ZP. In regards to the role of the cumulus cells, it was found that cumulus-enclosed oocytes had a higher incidence of fertilisation compared to cumulus-free oocytes [1]. Moreover, cumulus-free oocytes had an increased incidence of in vitro fertilisation when incubated with other cumulus-enclosed cells; this finding suggests that cumulus cells harbour an important role in fertilisation [1]. However, it is notable that when cumulus-free oocytes were incubated in a cumulus-conditioned medium, no increase in fertilisation rate was noted[1]. Overall, two conclusions were made: firstly, that the AR is required by the spermatozoa prior to meeting the ZP for effective fertilisation[1]. Secondly, the cumulus oophorus confers benefit in increasing the chance of fertilisation[1].


References

  1. 1.0 1.1 1.2 1.3 1.4 1.5 1.6 1.7 1.8 1.9 <pubmed>21383182</pubmed>


Lab 2 Assessment

1. Upload an image from a journal source relating to fertilization or the first 2 weeks of development as demonstrated in the practical class. Including in the image “Summary” window: An image name as a section heading, Any further description of what the image shows, A subsection labeled “Reference” and under this the original image source, appropriate reference and all copyright information and finally a template indicating that this is a student image.

Patterns of ZPC Deposition in Porcine Oocyte-Cumulus Complexes

Patterns of ZPC Deposition in Porcine Oocytes.jpg

Immunofluorescence Detection for ZPC and Ubiquitin in a Porcine Oocyte [1]

References

  1. <pubmed>21383844</pubmed>


2. Identify a protein associated with the implantation process, including a brief description of the protein's role (1-2 paragraphs).

Trophinin and Implantation

Trophinin is a membrane protein expressed in chorionic villi trophoblasts and in the maternal endometrium. In the early stages of pregnancy, trophinin is strongly expressed along with tastin and bystin, which form a complex; this complex mediates apical cell adhesion between the trophoblasts and the endometrial epithelial cells[1]. The time frame in which trophinin is expressed on the apical aspect of the endometrial cells coincides with the "implantation window"[1]; the period in which successful implantation is possible. Trophonin-trophonin adhesion during implantation occurs via signal transduction with bystin and tastin[2]. As a consequence of trophinin-trophinin adhesion, trophectoderm cells become activated for implantation[2]. Moreover, there have been reports that endometrial epithelial cells undergo apoptosis upon blastocyst adhesion; human trophoectoderm cells express the Fas ligand which interacts with Fas expressed on the endometrium[2]. However, other studies have shown that trophinin-mediated cell adhesion can induce endometrial cell apoptosis through mechanisms other than the Fas/FasL cascade[2].

In regards to ectopic pregnancies located within the fallopian tube, research has shown that trophinin is strongly expressed by both the embryonic trophoblasts and maternal fallopian tube epithelium, induced by human chorionic gonadotrophin (hCG)[1]. These findings highlight the function of trophonin in facilitating implantation in conjunction with its role in the pathogenesis of ectopic pregnancies.

References

  1. 1.0 1.1 1.2 <pubmed>14633596</pubmed>
  2. 2.0 2.1 2.2 2.3 <pubmed>22201876</pubmed>


Lab 3 Assessment

1. Identify the difference between "gestational age" and "post-fertilisation age" and explain why clinically "gestational age" is used in describing human development.

Gestational Age versus Post-Fertilisation Age

Gestation is the period of time between conception and birth (Kaneshiro, 2011; Vishton, 2011). Gestational age is the developmental age of the conceptus based on the presumed first day of the last normal menstrual period to the current date, measured in weeks (Kaneshiro, 2011; Vishton, 2011). In contrast, post-fertilisation age refers to the age of the conceptus expressed in elapsed time since fertilisation (Vishton, 2011). Gestational age is approximately two weeks greater than post-fertilization age (Kaneshiro, 2011; Vishton, 2011). Gestational age is used in human development because its start date can be determined by asking the mother when was the presumed first day of the last normal menstrual period (Kaneshiro, 2011; Vishton, 2011). In contrast, the moment of fertilization must be inferred (Vishton, 2011).


References

Kaneshiro, N. K. (2011). Gestational age. Retrieved from http://www.umm.edu/ency/article/002367.htm

Vishton, P. M. (2011). Embryo Foetus Development Stages. Retrieved from http://www.livestrong.com/article/92683-embryo-fetus-development-stages/


2.Identify using histological descriptions at least 3 different types of tissues formed from somites.

Tissues Derived From Somites

1. Bone (Sclerotome)

Bone tissue consists of cells separated by an extracellular matrix with organic and inorganic components (Marieb, Wilhem & Mallatt, 2010). The organic components of bone consists of cells, collagen fibres and ground substance. The cells include osteoprogenitor cells which give rise to osteoblasts: the producers of new bone matrix (osteoid). Mature osteoblasts, called osteocytes, are trapped in lacunae where they maintain the mature bone; osteocytes may revert to osteoblasts in the incidence of a fracture. Osteoclasts are multinucleated cells with ruffled plasma membrane borders and are involved in bone resorption. Bone resorption is important for bone remodelling to improve tensile strength as well as remodel the newly deposited woven bone into mature bone after a fracture. There are two types of mature (lamellar) bone: compact bone and spongy bone (Marieb et al., 2010). The compact bone occurs towards the periphery and is arranged in Haversian systems: lamellae concentrically arranged around a central Haversian canal containing blood vessels, nerves and osteocytes (Marieb et al., 2010). The different Haversian systems communicate with each other, the periosteum and endosteum through the Volkmann's canals. In contrast, spongy bone in the mature adult appears towards the centre of the diaphysis and metaphysis and is arranged in bony shelves (trabeculae) (Marieb et al., 2010). The gross porous arrangement of spongy bone is important for housing the bone marrow (Marieb et al., 2010)


2. The Skin (Dermotome)

a. Dermis: The dermis is made up of two main regions: (1) the superficial papillary layer and (2) the deeper reticular layer (Marieb et al., 2010). The superficial papillary layer makes up 20% of the dermis and is areolar connective tissue consisting of collagen and elastic fibres; it includes the dermal papillae which extend into the overlying epidermis to strengthen the dermal-epidermal junction and increase surface area for nutrient, gas and waste exchange with the avascular epidermis (Marieb et al., 2010). The reticular layer is composed of dense irregular connective tissue with thick bundles of collagen and elastic fibres arranged in different planes (Marieb et al., 2010). Other cells interspersed among the connective tissue of the dermis include fibroblasts, macrophages, mast cells and other white blood cells including lymphocytes(Marieb et al., 2010). The dermis is highly vascular and supplied with nerve fibres (Marieb et al., 2010). There are two vascular plexuses; the deep dermal plexus and the subpapillary plexus (Marieb et al., 2010). These vessels serve not only for nutrient supply to the dermis and epidermis, but for temperature regulation as well (Marieb et al., 2010).

b. Hypodermis: The subcutaneous layer, or fatty hypodermis, consists of areolar and adipose connective tissue(Marieb et al., 2010). The cellular components include adipocytes as well as white blood cells (Marieb et al., 2010). The hypodermis serves to store fat and anchor the skin to underlying structure in a manner that the skin can slide over structures(Marieb et al., 2010). Additionally, the adipose in the hypodermis serves an insulator.


3. Skeletal Muscle (Myotome) Skeletal muscle fibres come together to form a larger skeletal muscle surrounded by different levels of connective tissue "coats": the epimysium surrounds the whole skeletal muscle, the perimysium covers each fascicle and the loose CT endomysium separates each skeletal muscle fibre (Marieb et al., 2010). The skeletal muscle fibres are long cylindrical cells with a diameter between 10-100um (Marieb et al., 2010) . These muscle fbres are formed by the fusion of embryonic cells and hence contain many nuclei which are located at the periphery of each fibre beneath the sarcolemma, the skeletal muscle cell membrane (Marieb et al., 2010). These muscle fibres appear striated because of the internal organelles of the muscle fibres: myofibrils, the contractile organelles of muscle tissue (Marieb et al., 2010).


References

Marieb, E. N., Wilhelm, P. B., Mallatt, J. (2010). Human Anatomy (6th ed.). San Francisco, CA: Pearson Education, Inc.


Lab 4 Assessment

Prenatal Diagnostic Techniques

1. Identify the 2 invasive prenatal diagnostic techniques related to the placenta and 2 abnormalities that can be identified with these techniques.

  • Techniques:
    • Amniocentesis [1]: Amniocentesis refers to sampling of the amniotic fluid by inserting a needle through the mother's anterior abdominal and uterine walls into the amniotic cavity by piercing the chorion and amnion. This technique is performed at 15 and 18 weeks gestation.
    • Chorionic Villus Sampling [2]: Biopsies of trophoblastic tissue are obtained by inserting a needle through the mother's abdominal wall and uterine walls to the uterine cavity. Sampling can also be performed through the cervix with a polyethylene catheter, guided by real-time ultrasonography. CVS can be performed sooner than amniocentesis at 10 and 12 weeks of gestation. However, the rate of miscarriage from CVS is higher than amniocentesis.
  • Abnormalities which can be identified:
  1. Down Syndrome (Trisomy 21): Amniocentesis and CVS allow for chromosome abnormality detection. [3]
  2. Tay-Sachs [4]


The Therapeutic Use of Cord Stem Cells

2. Identify a paper that uses cord stem cells therapeutically and write a brief (2-3 paragraph) description of the paper's findings.


References

  1. Moore, K. L., Persaud, T. V. N. & Torchia, M. G. (2013). The Developing Human (9th ed.). Philadelphia, PA: Elsevier Saunders.
  2. Moore, K. L., Persaud, T. V. N. & Torchia, M. G. (2013). The Developing Human (9th ed.). Philadelphia, PA: Elsevier Saunders.
  3. Moore, K. L., Persaud, T. V. N. & Torchia, M. G. (2013). The Developing Human (9th ed.). Philadelphia, PA: Elsevier Saunders.
  4. Sheth, K. (2012). Tay-Sachs Disease. Retrieved from http://www.nlm.nih.gov/medlineplus/ency/article/001417.htm.

Lab Attendance

Lab 1 --Z3333038 11:49, 25 July 2012 (EST)

Lab 2 --Z3333038 10:05, 1 August 2012 (EST)

Lab 3 --Z3333038 10:01, 8 August 2012 (EST)

Lab 4 --Z3333038 10:00, 15 August 2012 (EST)