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From Embryology

Lab Attendence

Lab 2 --Z3332885 10:38, 1 August 2012 (EST)

Lab 1 Assessment

1)Identify the origin of In Vitro Fertilization and the 2010 nobel prize winner associated with this technique and add a correctly formatted link to the Nobel page

Research into controlling fertility has been going on for a while, and many experiments for IVF has been conducted since the 1960s. In 1973 the first IVF pregnancy was achieved, though baby did not survive till birth. In the 1978 the first IVF baby was born in india. The baby named "Durga" was the work of physican Kolkata using primitive methods and household refrigeration. In 1979 the world’s second baby conceived by IV was born.


In 2010, the Nobel Prize for physiology medicine was awarded to Robert G. Edwards for his contribution in the development of In Vitro Fertilization. [1]


(2)Identify and add a PubMed reference link to a recent paper on fertilisation and describe its key findings (1-2 paragraphs)

Summary

Many embryos are discarded from IVF labratories because they are considered to be poor quality. These embryos are, however, proposed to be good sources for human embryonic stem cells (heSC). This article studied whether heSC could be isolated from poor quality embryos, while also evaluating the efficiency of different isolation methods. Their experiments involved 166 embryos. The embryos were culture in a blastocyst medium for 2 days so that inner cell masses could develope. The inner masses from 32 embryo cells were then isolated, 17 using a mechanical method, the other 15 by immunosurgery. The isolated ICMs were subcultivated, and the rates of ICM colony formation was measured. Both methods had similar success rate (P>0.05). However, the cultures that did work had normal hESC differentiation ability, which indicates heSC could be derived from poor embryos. [2]

References

   1.http://en.wikipedia.org/wiki/In_vitro_fertilisation
   2.Liu W, Yin Y, Long X, Luo Y, Jiang Y, Zhang W, Du H, Li S, Zheng Y, Li Q, Chen X, Liao B, Xiao G, Wang W, Sun X.
     Derivation and characterization of human embryonic stem cell lines from poor quality embryos. J Genet Genomics. 2009 PMID: 19376483