User:Z3332885: Difference between revisions
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Their experiments involved 166 embryos. The embryos were culture in a blastocyst medium for 2 days so that inner cell masses could develope. The inner masses from 32 embryo cells were then isolated, 17 using a mechanical method, the other 15 by immunosurgery. | Their experiments involved 166 embryos. The embryos were culture in a blastocyst medium for 2 days so that inner cell masses could develope. The inner masses from 32 embryo cells were then isolated, 17 using a mechanical method, the other 15 by immunosurgery. | ||
The isolated ICMs were subcultivated, and the rates of ICM colony formation was measured. Both methods had similar success rate (P>0.05). | The isolated ICMs were subcultivated, and the rates of ICM colony formation was measured. Both methods had similar success rate (P>0.05). | ||
However, the cultures that did work had normal hESC differentiation ability, which indicates heSC could be derived from poor embryos. | However, the cultures that did work had normal hESC differentiation ability, which indicates heSC could be derived from poor embryos. [http://www.ncbi.nlm.nih.gov/pubmed/19376483 PubMed] | ||
Revision as of 19:41, 31 July 2012
Lab 1 Assessment
1)Identify the origin of In Vitro Fertilization and the 2010 nobel prize winner associated with this technique and add a correctly formatted link to the Nobel page
Research into controlling fertility has been going on for a while, and many experiments for IVF has been since the 1960s.
In 2010, the Nobel Prize for physiology medicine was awarded to Robert G. Edwards for his contribution in the development of In Vitro Fertilization. [1]
(2)Identify and add a PubMed reference link to a recent paper on fertilisation and describe its key findings (1-2 paragraphs)
Summary
Many embryos are discared from IVF labratories because they are considered to be poor quality. These embryos are, however, proposed to be good sources for human embryonic stem cells (heSC). This article studied whether heSC could be isolated from poor quality embryos, while also evaluating the efficiency of different isolation methods. Their experiments involved 166 embryos. The embryos were culture in a blastocyst medium for 2 days so that inner cell masses could develope. The inner masses from 32 embryo cells were then isolated, 17 using a mechanical method, the other 15 by immunosurgery. The isolated ICMs were subcultivated, and the rates of ICM colony formation was measured. Both methods had similar success rate (P>0.05). However, the cultures that did work had normal hESC differentiation ability, which indicates heSC could be derived from poor embryos. PubMed
