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Lab Attendance

Lab 1 --Z3220343 11:49, 25 July 2012 (EST) Lab 2 --Z3220343 10:56, 1 August 2012 (EST) Lab 3 --Z3220343 10:06, 8 August 2012 (EST)


Lab 1 Assignment

    • The history of IVF dates back to the 1890's with Walter Heape reporting embryo transplants in rabbits. It was not until 1965 that Robert Edwards, Georgianna and Howard Jones, attempted to fertilize human oocytes In Vitro. In 1973 the first IVF pregnancy was reported by Leeton and Wood at Monash University (Melbourne, Australia) but this resulted in a miscarriage. The first IVF birth was the famous Louise Brown, born in 1978. This birth was the result of the collaborate works of Robert Edwards and Patrick Steptoe. [1]
  • In 2010 Robert Edwards won the Nobel Prize in Physiology or Medicine for his work with In Vitro Fertilization. [2]

Demián Glujovsky, Debbie Blake, Cindy Farquhar ,Ariel Bardach. Cleavage stage versus blastocyst stage embryo transfer in assisted reproductive technology 11 July 2012

The purpose of this article is to determine whether implantation of a ‘cleavage stage’(2-3 days) embryo is more likely to result in a live birth or if implantation should occur later when the embryo has reached the ‘blastocysts’ (5-6 days) stage of development. While there have been papers published on the success of ‘blastocysts’ implantation, it is noted that these trials were not randomised nor a consensus about the best practice for blastocyst culture. This paper delves into the results of a clinical randomised trial on blastocysts against cleavage stage. The merits found towards blastocysts are based on 2 arguments. 1. That most successful pregnancies in vivo, the embryo does not reach the uterus at day 3, which is the typical implantation time for cleavage transfer. 2. That blastocysts have higher implantation potential as the embryo have reach the blastocysts stage, have the most potential to survive. It is this second point, the one of self-selection which is seen a negative in the use of blastocysts implantation. This study found that although the live birth rate for the randomised controlled trail for blastocysts was a small but significant difference, the miscarriage rate between cleavage stage and blastocysts remained the same. On a whole this paper found that the benefits between cleavage stage and blastocysts are still unclear. [3]


Lab 2 Assignment

  1. Image:Fly GenomeJournal.pbio.0050131.g001.png[1]
  2. Trophinin is a protein that is expressed by trophoblast cells. In vivo pattern and activity of this protein suggests that it assists in the initial attachment of trophectoderm cells into the maternal epithelia. It is also instrumental in allowing the trophoblasts to invade through the uterine wall. [2]

References

  1. Gross L (2007) Novel Technique Shows When the Fertilized Egg’s Genome Comes into Its Own. PLoS Biol 5(5): e131. doi:10.1371/journal.pbio.0050131
  2. <pubmed>17487845</pubmed>