Template:Pincus1935 table 2
From Embryology
Table 2 - Data on Ovarian Eggs Obtained by Puncture of Graafian Follicles, and Cultured in (a) Media Containing Pituitary Hormones, (b) Media of Ringer-Locke Solution | |||
Time of culturing | No. of cultures | Medium | Results |
---|---|---|---|
20 (min.) | 14 | Ringer-Locke + 1 drop beef pituitary | Vesicular tetrads in all cases |
2 (hrs.) | 11 | Ringer-Locke + 2 drops beef pituitary | In some cases vesicular tetrads and some free tetrads were formed. Some formed polar bodies |
24 | 9 | Ringer-Locke + 1 drop maturity hormone | Vesicular tetrads in all cases, except 3 which had free tetrads |
25 | 4 | Ringer-Locke + 2 drops maturity hormone | Vesicular tetrads formed in all cases |
25 | 7 | Ringer-Locke + 3 drops maturity hormone | Vesicular tetrads, free tetrads, structures resembling fusion nuclei |
2 | 18 | Ringer-Locke | Vesicular tetrads and free tetrads |
4 | 3 | Ringer-Locke | Free tetrads |
6 | 3 | Ringer-Locke | Rudiment of first polar spindle |
20 | 16 | Ringer-Locke | Vesicular tetrads, free tetrads, fusion nuclei |