Template:Pincus1935 table 2

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Table 2 - Data on Ovarian Eggs Obtained by Puncture of Graafian Follicles, and Cultured in (a) Media Containing Pituitary Hormones, (b) Media of Ringer-Locke Solution
Time of culturing No. of cultures Medium Results
20 (min.) 14 Ringer-Locke + 1 drop beef pituitary Vesicular tetrads in all cases
2 (hrs.) 11 Ringer-Locke + 2 drops beef pituitary In some cases vesicular tetrads and some free tetrads were formed. Some formed polar bodies
24 9 Ringer-Locke + 1 drop maturity hormone Vesicular tetrads in all cases, except 3 which had free tetrads
25 4 Ringer-Locke + 2 drops maturity hormone Vesicular tetrads formed in all cases
25 7 Ringer-Locke + 3 drops maturity hormone Vesicular tetrads, free tetrads, structures resembling fusion nuclei
2 18 Ringer-Locke Vesicular tetrads and free tetrads
4 3 Ringer-Locke Free tetrads
6 3 Ringer-Locke Rudiment of first polar spindle
20 16 Ringer-Locke Vesicular tetrads, free tetrads, fusion nuclei