Talk:Zona pellucida: Difference between revisions

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==2011==
===Biosynthesis of hamster zona pellucida is restricted to the oocyte===
Theriogenology. 2011 Feb;75(3):463-72. Epub 2010 Nov 12.
Izquierdo-Rico MJ, Gimeno L, Jiménez-Cervantes C, Ballesta J, Avilés M.
Department of Cell Biology and Histology, Faculty of Medicine, University of Murcia, Murcia, Spain.
Abstract
The zona pellucida (ZP) is an extracellular coat that surrounds the mammalian oocyte and the early embryo until implantation. This coat mediates several critical aspects of fertilization, including species-selective sperm recognition, the blocking of polyspermy and protection of the oocyte and the preimplantation embryo. Depending on the species, the ZP is composed of three to four different glycoproteins encoded by three or four genes. These genes have been cloned and sequenced for different species. However, controversy exists about the cell type specificity of the ZP glycoproteins, for which several models have been proposed. Different groups have reported that ZP is produced only by the oocytes, by the granulosa cells or by both cell types, depending on the species under study. We recently described the expression of four ZP proteins in the hamster ovary. By means of the complete set of the hamster ZP cDNAs, we undertook the study of the origin and expression pattern of the four ZP genes. In the present work, the expression of ZP1, ZP2, ZP3 and ZP4 is carefully analyzed by in situ hybridization (ISH) in hamster ovaries. Our data suggest that the four hamster ZP genes are expressed in a coordinate and oocyte-specific manner during folliculogenesis. Furthermore, this expression is maximal during the first stages of the oocyte development and declines in oocytes from later development stages, particularly within large antral follicles.
Copyright © 2011 Elsevier Inc. All rights reserved.
http://www.ncbi.nlm.nih.gov/pubmed/21074836
PMID: 21074836


==2006==
==2006==

Revision as of 09:37, 25 February 2011

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Cite this page: Hill, M.A. (2024, March 28) Embryology Zona pellucida. Retrieved from https://embryology.med.unsw.edu.au/embryology/index.php/Talk:Zona_pellucida

2011

Biosynthesis of hamster zona pellucida is restricted to the oocyte

Theriogenology. 2011 Feb;75(3):463-72. Epub 2010 Nov 12.

Izquierdo-Rico MJ, Gimeno L, Jiménez-Cervantes C, Ballesta J, Avilés M. Department of Cell Biology and Histology, Faculty of Medicine, University of Murcia, Murcia, Spain.

Abstract

The zona pellucida (ZP) is an extracellular coat that surrounds the mammalian oocyte and the early embryo until implantation. This coat mediates several critical aspects of fertilization, including species-selective sperm recognition, the blocking of polyspermy and protection of the oocyte and the preimplantation embryo. Depending on the species, the ZP is composed of three to four different glycoproteins encoded by three or four genes. These genes have been cloned and sequenced for different species. However, controversy exists about the cell type specificity of the ZP glycoproteins, for which several models have been proposed. Different groups have reported that ZP is produced only by the oocytes, by the granulosa cells or by both cell types, depending on the species under study. We recently described the expression of four ZP proteins in the hamster ovary. By means of the complete set of the hamster ZP cDNAs, we undertook the study of the origin and expression pattern of the four ZP genes. In the present work, the expression of ZP1, ZP2, ZP3 and ZP4 is carefully analyzed by in situ hybridization (ISH) in hamster ovaries. Our data suggest that the four hamster ZP genes are expressed in a coordinate and oocyte-specific manner during folliculogenesis. Furthermore, this expression is maximal during the first stages of the oocyte development and declines in oocytes from later development stages, particularly within large antral follicles.

Copyright © 2011 Elsevier Inc. All rights reserved.

http://www.ncbi.nlm.nih.gov/pubmed/21074836

PMID: 21074836

2006

Three-dimensional structure of the zona pellucida at ovulation

Microsc Res Tech. 2006 Jun;69(6):415-26.

Familiari G, Relucenti M, Heyn R, Micara G, Correr S. Laboratory of Electron Microscopy, Pietro M. Motta, Department of Anatomy, University of Rome La Sapienza, 00161 Rome, Italy. giuseppe.familiari@uniroma1.it

Abstract

The mammalian zona pellucida (ZP) is an extracellular matrix surrounding oocytes and early embryos, which is critical for normal fertilization and preimplantation development. It is made up of three/four glycoproteins arranged in a delicate filamentous matrix. Scanning electron microscopy (SEM) studies have shown that ZP has a porous, net-like structure and/or nearly smooth and compact aspect. In this study, the fine 3-D structure of the human and mouse ZP is reviewed with the aim to integrate ultrastructural and molecular data, considering that the mouse is still used as a good model for human fertilization. By conventional SEM observations, numerous evidences support that the spongy ZP appearance well correlates with mature oocytes. When observed through more sophisticated techniques at high resolution SEM, ZP showed a delicate meshwork of thin interconnected filaments, in a regular alternating pattern of wide and tight meshes. In mature oocytes, the wide meshes correspond to "pores" of the "spongy" ZP, whereas the tight meshes correspond to the compact parts of the ZP surrounding the pores. In conclusion, the traditional "spongy" or "compact" appearance of the ZP at conventional SEM appears to be only the consequence of a prevalence of different arrangements of microfilament networks, according to the maturation stage of the oocyte, and in agreement with the modern supramolecular model of the ZP at the basis of egg-sperm recognition. Despite great differences in molecular characterization of ZP glycoproteins between human and mouse ZP, there are no differences in the 3-D organization of glycoproteic microfilaments in these species.

PMID: 16703610 http://www.ncbi.nlm.nih.gov/sites/entrez/16703610



http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2582072/?tool=pmcentrez

http://www.springer.com/open+access/open+choice?SGWID=0-40359-12-683308-0

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2528931/?tool=pmcentrez

American Society for Microbiology -

http://mcb.asm.org/

http://www.asm.org/index.php?option=com_content&view=article&id=90986