Talk:Musculoskeletal System - Joint Development: Difference between revisions
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:"Hunziker et al. [47] showed that AC grows appositionally. The superficial zone supplies the stem cells for AC growth. Daughter cells that are displaced horizontally, remain confined to the superficial zone and replenish the stem-cell pool and affect lateral growth. Daughter cells that move vertically downwards form a zone with a rapidly dividing and proliferating pool of cells for rapid clonal expansion. This zone affects longitudinal growth and is located at the transitional and upper deep layer of AC [47]. The location (distance from the articular surface) of the collagen density valley in our study appears to coincide with the zone of rapidly dividing daughter cells in the study by Hunziker et al. [47]. Hunziker et al. further showed that the proliferation activity of this pool of cells decreased with age and had ceased when AC thickness stabilised. The valley in collagen density that we observe in our study also gradually disappears with age, and also stabilises when cartilage thickness stabilises (36 weeks, figure 5c). These similarities in the spatial and temporal patterns of cell proliferation and the presence of a collagen density valley, suggest a relationship between the cell activity and collagen production in this zone. Dedicated investigations will be required to show whether or not such a relationship exists." | |||
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Cite this page: Hill, M.A. (2024, April 18) Embryology Musculoskeletal System - Joint Development. Retrieved from https://embryology.med.unsw.edu.au/embryology/index.php/Talk:Musculoskeletal_System_-_Joint_Development |
2010
Postnatal development of depth-dependent collagen density in ovine articular cartilage
BMC Dev Biol. 2010 Oct 22;10:108.
van Turnhout MC, Schipper H, van Lagen B, Zuilhof H, Kranenbarg S, van Leeuwen JL.
Experimental Zoology Group, Department of Animal Sciences, Wageningen University, PO Box 338, 6700 AH, Wageningen, The Netherlands. m.c.v.turnhout@tue.nl
Abstract
BACKGROUND: Articular cartilage (AC) is the layer of tissue that covers the articulating ends of the bones in diarthrodial joints. Adult AC is characterised by a depth-dependent composition and structure of the extracellular matrix that results in depth-dependent mechanical properties, important for the functions of adult AC. Collagen is the most abundant solid component and it affects the mechanical behaviour of AC. The current objective is to quantify the postnatal development of depth-dependent collagen density in sheep (Ovis aries) AC between birth and maturity. We use Fourier transform infra-red micro-spectroscopy to investigate collagen density in 48 sheep divided over ten sample points between birth (stillborn) and maturity (72 weeks). In each animal, we investigate six anatomical sites (caudal, distal and rostral locations at the medial and lateral side of the joint) in the distal metacarpus of a fore leg and a hind leg.
RESULTS: Collagen density increases from birth to maturity up to our last sample point (72 weeks). Collagen density increases at the articular surface from 0.23 g/ml ± 0.06 g/ml (mean ± s.d., n = 48) at 0 weeks to 0.51 g/ml ± 0.10 g/ml (n = 46) at 72 weeks. Maximum collagen density in the deeper cartilage increases from 0.39 g/ml ± 0.08 g/ml (n = 48) at 0 weeks to 0.91 g/ml ± 0.13 g/ml (n = 46) at 72 weeks. Most collagen density profiles at 0 weeks (85%) show a valley, indicating a minimum, in collagen density near the articular surface. At 72 weeks, only 17% of the collagen density profiles show a valley in collagen density near the articular surface. The fraction of profiles with this valley stabilises at 36 weeks.
CONCLUSIONS: Collagen density in articular cartilage increases in postnatal life with depth-dependent variation, and does not stabilize up to 72 weeks, the last sample point in our study. We find strong evidence for a valley in collagen densities near the articular surface that is present in the youngest animals, but that has disappeared in the oldest animals. We discuss that the retardance valley (as seen with polarised light microscopy) in perinatal animals reflects a decrease in collagen density, as well as a decrease in collagen fibril anisotropy.
PMID: 20969753 http://www.ncbi.nlm.nih.gov/pubmed/20969753
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2987790
http://www.biomedcentral.com/1471-213X/10/108
- "Hunziker et al. [47] showed that AC grows appositionally. The superficial zone supplies the stem cells for AC growth. Daughter cells that are displaced horizontally, remain confined to the superficial zone and replenish the stem-cell pool and affect lateral growth. Daughter cells that move vertically downwards form a zone with a rapidly dividing and proliferating pool of cells for rapid clonal expansion. This zone affects longitudinal growth and is located at the transitional and upper deep layer of AC [47]. The location (distance from the articular surface) of the collagen density valley in our study appears to coincide with the zone of rapidly dividing daughter cells in the study by Hunziker et al. [47]. Hunziker et al. further showed that the proliferation activity of this pool of cells decreased with age and had ceased when AC thickness stabilised. The valley in collagen density that we observe in our study also gradually disappears with age, and also stabilises when cartilage thickness stabilises (36 weeks, figure 5c). These similarities in the spatial and temporal patterns of cell proliferation and the presence of a collagen density valley, suggest a relationship between the cell activity and collagen production in this zone. Dedicated investigations will be required to show whether or not such a relationship exists."
