Talk:Mouse Development

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Cite this page: Hill, M.A. (2024, March 28) Embryology Mouse Development. Retrieved from https://embryology.med.unsw.edu.au/embryology/index.php/Talk:Mouse_Development

original page | Mouse Stages | Mouse Timeline (day by day) | Mouse Detailed Timeline (day by day) | Mouse Estrous Cycle | Mouse Heart


10 Most Recent Papers

Note - This sub-heading shows an automated computer PubMed search using the listed sub-heading term. References appear in this list based upon the date of the actual page viewing. Therefore the list of references do not reflect any editorial selection of material based on content or relevance. In comparison, references listed on the content page and discussion page (under the publication year sub-headings) do include editorial selection based upon relevance and availability. (More? Pubmed Most Recent)


Mouse Development

<pubmed limit=5>Mouse Development</pubmed>

Mouse Embryology

<pubmed limit=5>Mouse Embryology</pubmed>

2013

2012

4D imaging reveals a shift in chromosome segregation dynamics during mouse pre-implantation development

Cell Cycle. 2012 Dec 19;12(1). [Epub ahead of print]

Yamagata K, Fitzharris G. Source Research Institute for Microbial Diseases; Osaka University; Suita, Osaka Japan.

Abstract

Cells of the early developing mammalian embryo frequently mis-segregate chromosomes during cell division, causing daughter cells to inherit an erroneous numbers of chromosomes. Why the embryo is so susceptible to errors is unknown, and the mechanisms that embryos employ to accomplish chromosome segregation are poorly understood. Chromosome segregation is performed by the spindle, a fusiform-shaped microtubule-based transient organelle. Here we present a detailed analysis of 4D fluorescence-confocal data sets of live embryos progressing from the one-cell embryo stage through to blastocyst in vitro, providing some of the first mechanistic insights into chromosome segregation in the mammalian embryo. We show that chromosome segregation occurs as a combined result of poleward chromosome motion (anaphase-A) and spindle elongation (anaphase-B), which occur simultaneously at the time of cell division. Unexpectedly, however, regulation of the two anaphase mechanisms changes significantly between the first and second embryonic mitoses. In one-cell embryos, the velocity of anaphase-A chromosome motion and the velocity and overall extent of anaphase-B spindle elongation are significantly constrained compared with later stages. As a result, chromosomes are delivered close to the center of the forming two-cell stage blastomeres at the end of the first mitosis. In subsequent divisions, anaphase-B spindle elongation is faster and more extensive, resulting in the delivery of chromosomes to the distal plasma membrane of the newly forming blastomeres. Metaphase spindle length scales with cell size from the two-cell stage onwards, but is substantially shorter in the first mitosis than in the second mitosis, and the duration of mitosis-1 is substantially greater than subsequent divisions. Thus, there is a striking and unexpected shift in the approach to cell division between the first and second mitotic divisions, which likely reflects adaptations to the unique environment within the developing embryo.

PMID 23255117

Application of in utero electroporation and live imaging in the analyses of neuronal migration during mouse brain development

Med Mol Morphol. 2012 Dec;45(1):1-6. Epub 2012 Mar 20.

Nishimura YV, Shinoda T, Inaguma Y, Ito H, Nagata K. Source Department of Molecular Neurobiology, Institute for Developmental Research, Aichi Human Service Center, 713-8 Kamiya, Kasugai, Aichi, 480-0392, Japan.

Abstract

Correct neuronal migration is crucial for brain architecture and function. During cerebral cortex development (corticogenesis), excitatory neurons generated in the proliferative zone of the dorsal telencephalon (mainly ventricular zone) move through the intermediate zone and migrate past the neurons previously located in the cortical plate and come to rest just beneath the marginal zone. The in utero electroporation technique is a powerful method for rapid gain- and loss-of-function studies of neuronal development, especially neuronal migration. This method enabled us to introduce genes of interest into ventricular zone progenitor cells of mouse embryos and to observe resulting phenotypes such as proliferation, migration, and cell morphology at later stages. In this Award Lecture Review, we focus on the application of the in utero electroporation method to functional analyses of cytoskeleton-related protein septin. We then refer to, as an advanced technique, the in utero electroporation-based real-time imaging method for analyses of cell signaling regulating neuronal migration. The in utero electroporation method and its application would contribute to medical molecular morphology through identification and characterization of the signaling pathways disorganized in various neurological and psychiatric disorders.

PMID 22431177

Cell fate decisions and axis determination in the early mouse embryo

Development. 2012 Jan;139(1):3-14.

Takaoka K, Hamada H. Source Developmental Genetics Group, Graduate School of Frontier Biosciences, Osaka University, 1-1 Yamada-oka, Suita, Osaka 565-0871, Japan.

Abstract

The mouse embryo generates multiple cell lineages, as well as its future body axes in the early phase of its development. The early cell fate decisions lead to the generation of three lineages in the pre-implantation embryo: the epiblast, the primitive endoderm and the trophectoderm. Shortly after implantation, the anterior-posterior axis is firmly established. Recent studies have provided a better understanding of how the earliest cell fate decisions are regulated in the pre-implantation embryo, and how and when the body axes are established in the pregastrulation embryo. In this review, we address the timing of the first cell fate decisions and of the establishment of embryonic polarity, and we ask how far back one can trace their origins.

PMID 22147950

2011

A conditional knockout resource for the genome-wide study of mouse gene function

Nature. 2011 Jun 15;474(7351):337-42. doi: 10.1038/nature10163.

Skarnes WC, Rosen B, West AP, Koutsourakis M, Bushell W, Iyer V, Mujica AO, Thomas M, Harrow J, Cox T, Jackson D, Severin J, Biggs P, Fu J, Nefedov M, de Jong PJ, Stewart AF, Bradley A. Source Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1SA, UK. skarnes@sanger.ac.uk

Abstract

Gene targeting in embryonic stem cells has become the principal technology for manipulation of the mouse genome, offering unrivalled accuracy in allele design and access to conditional mutagenesis. To bring these advantages to the wider research community, large-scale mouse knockout programmes are producing a permanent resource of targeted mutations in all protein-coding genes. Here we report the establishment of a high-throughput gene-targeting pipeline for the generation of reporter-tagged, conditional alleles. Computational allele design, 96-well modular vector construction and high-efficiency gene-targeting strategies have been combined to mutate genes on an unprecedented scale. So far, more than 12,000 vectors and 9,000 conditional targeted alleles have been produced in highly germline-competent C57BL/6N embryonic stem cells. High-throughput genome engineering highlighted by this study is broadly applicable to rat and human stem cells and provides a foundation for future genome-wide efforts aimed at deciphering the function of all genes encoded by the mammalian genome.

PMID: 21677750 http://www.ncbi.nlm.nih.gov/pubmed/21677750

http://www.nature.com/nature/journal/v474/n7351/full/nature10163.html

A high-resolution anatomical atlas of the transcriptome in the mouse embryo

PLoS Biol. 2011 Jan 18;9(1):e1000582.

Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, Magen A, Canidio E, Pagani M, Peluso I, Lin-Marq N, Koch M, Bilio M, Cantiello I, Verde R, De Masi C, Bianchi SA, Cicchini J, Perroud E, Mehmeti S, Dagand E, Schrinner S, Nürnberger A, Schmidt K, Metz K, Zwingmann C, Brieske N, Springer C, Hernandez AM, Herzog S, Grabbe F, Sieverding C, Fischer B, Schrader K, Brockmeyer M, Dettmer S, Helbig C, Alunni V, Battaini MA, Mura C, Henrichsen CN, Garcia-Lopez R, Echevarria D, Puelles E, Garcia-Calero E, Kruse S, Uhr M, Kauck C, Feng G, Milyaev N, Ong CK, Kumar L, Lam M, Semple CA, Gyenesei A, Mundlos S, Radelof U, Lehrach H, Sarmientos P, Reymond A, Davidson DR, Dollé P, Antonarakis SE, Yaspo ML, Martinez S, Baldock RA, Eichele G, Ballabio A.

Source

Telethon Institute of Genetics and Medicine, Naples, Italy.

Abstract

Ascertaining when and where genes are expressed is of crucial importance to understanding or predicting the physiological role of genes and proteins and how they interact to form the complex networks that underlie organ development and function. It is, therefore, crucial to determine on a genome-wide level, the spatio-temporal gene expression profiles at cellular resolution. This information is provided by colorimetric RNA in situ hybridization that can elucidate expression of genes in their native context and does so at cellular resolution. We generated what is to our knowledge the first genome-wide transcriptome atlas by RNA in situ hybridization of an entire mammalian organism, the developing mouse at embryonic day 14.5. This digital transcriptome atlas, the Eurexpress atlas (http://www.eurexpress.org), consists of a searchable database of annotated images that can be interactively viewed. We generated anatomy-based expression profiles for over 18,000 coding genes and over 400 microRNAs. We identified 1,002 tissue-specific genes that are a source of novel tissue-specific markers for 37 different anatomical structures. The quality and the resolution of the data revealed novel molecular domains for several developing structures, such as the telencephalon, a novel organization for the hypothalamus, and insight on the Wnt network involved in renal epithelial differentiation during kidney development. The digital transcriptome atlas is a powerful resource to determine co-expression of genes, to identify cell populations and lineages, and to identify functional associations between genes relevant to development and disease.

PMID: 21267068 http://www.ncbi.nlm.nih.gov/pubmed/21267068

http://www.plosbiology.org/article/info%3Adoi%2F10.1371%2Fjournal.pbio.1000582

Eurexpress transcriptome atlas http://www.eurexpress.org

2010

Making the blastocyst: lessons from the mouse

J Clin Invest. 2010 Apr;120(4):995-1003. doi: 10.1172/JCI41229. Epub 2010 Apr 1.

Cockburn K, Rossant J. Source Department of Molecular Genetics, University of Toronto, Canada.

Abstract

Mammalian preimplantation development, which is the period extending from fertilization to implantation, results in the formation of a blastocyst with three distinct cell lineages. Only one of these lineages, the epiblast, contributes to the embryo itself, while the other two lineages, the trophectoderm and the primitive endoderm, become extra-embryonic tissues. Significant gains have been made in our understanding of the major events of mouse preimplantation development, and recent discoveries have shed new light on the establishment of the three blastocyst lineages. What is less clear, however, is how closely human preimplantation development mimics that in the mouse. A greater understanding of the similarities and differences between mouse and human preimplantation development has implications for improving assisted reproductive technologies and for deriving human embryonic stem cells.

Image - Stages of mouse and human preimplantation development


PMID 20364097


2008

Developmental alveolarization of the mouse lung.

Dev Dyn. 2008 Aug;237(8):2108-16.

Mund SI, Stampanoni M, Schittny JC.

Institute of Anatomy, University of Bern, Switzerland.

Abstract Postnatal lung development is not well characterized in mice, especially the time point when alveolarization is completed. Using the total length and the length density of the free septal edge as measured for the formation of new septa, we followed alveolarization throughout postnatal lung development (days 2-125). Furthermore, the alveolar surface area was estimated. The formation of new septa was observed until day 36. Approximately 10% of the septa present in adult mice were formed prenatally by branching morphogenesis, approximately 50% were generated postnatally before and approximately 40% after maturation of the alveolar microvasculature. Approximately 5% of the alveolar surface area present during adulthood was present before alveolarization started, approximately 55% was formed during alveolarization (days 4-36) and approximately 40% afterward due to growth processes. We conclude that alveolarization continues until young adulthood and that the maturation of the alveolar microvasculature does not preclude further alveolarization.

PMID 18651668

In vivo quantification of embryonic and placental growth during gestation in mice using micro-ultrasound

Mu J, Slevin JC, Qu D, McCormick S, Adamson SL. Reprod Biol Endocrinol. 2008 Aug 12;6:34. PMID: 18700008

"RESULTS: Gestational sac dimension provided the earliest measure of conceptus size. Sac dimension derived using regression analysis increased from 0.84 mm at E7.5 to 6.44 mm at E11.5 when it was discontinued. The earliest measurement of embryo size was crown-rump length (CRL) which increased from 1.88 mm at E8.5 to 16.22 mm at E16.5 after which it exceeded the field of view. From E10.5 to E18.5 (full term), progressive increases were observed in embryonic biparietal diameter (BPD) (0.79 mm to 7.55 mm at E18.5), abdominal circumference (AC) (4.91 mm to 26.56 mm), and eye lens diameter (0.20 mm to 0.93 mm). Ossified femur length was measureable from E15.5 (1.06 mm) and increased linearly to 2.23 mm at E18.5. In contrast, placental diameter (PD) and placental thickness (PT) increased from E10.5 to E14.5 then remained constant to term in accord with placental weight. Ultrasound and light microscopy measurements agreed with no significant bias and a discrepancy of less than 25%. Regression equations predicting gestational age from individual variables, and embryonic weight (BW) from CRL, BPD, and AC were obtained. The prediction equation BW = -0.757 + 0.0453 (CRL) + 0.0334 (AC) derived from CD-1 data predicted embryonic weights at E17.5 in three other strains of mice with a mean discrepancy of less than 16%. "

Synaptogenesis in the mouse olfactory bulb during glomerulus development

Eur J Neurosci. 2008 Jun;27(11):2838-46.

Blanchart A, Romaguera M, García-Verdugo JM, de Carlos JA, López-Mascaraque L.

Department of Cellular, Molecular and Developmental Neurobiology, Instituto Cajal, CSIC, Madrid, Spain.

Synaptogenesis is essential for the development of neuronal networks in the brain. In the olfactory bulb (OB) glomeruli, numerous synapses must form between sensory olfactory neurons and the dendrites of mitral/tufted and periglomerular cells. Glomeruli develop from E13 to E16 in the mouse, coincident with an increment of the neuropil in the border between the external plexiform (EPL) and olfactory nerve layers (ONL), coupled to an extensive labelling of phalloidin and GAP-43 from the ONL to EPL. We have tracked synaptogenesis in the OB during this period by electron microscopy (EM) and immunolabelling of the transmembrane synaptic vesicle glycoprotein SV-2. No SV-2 labelling or synapses were detected at E13, although electrodense junctions lacking synaptic vesicles could be observed by EM. At E14, sparse SV-2 labelling appears in the most ventral and medial part of the incipient OB, which displays a ventro-dorsal gradient by E15 but covers the entire OB by E16. These data establish a spatio-temporal pattern of synaptogenesis, which perfectly matches with the glomeruli formation in developing OB.

PMID: 18588529

2007

Doppler ultrasound in mice

Echocardiography. 2007 Jan;24(1):97-112.

Stypmann J.

Department of Cardiology and Angiology, Hospital of the University of Münster, Germany. stypmann@mednet.uni-muenster.de Abstract Color, power, spectral, and tissue Doppler have been applied to mice. Due to the noninvasive nature of the technique, serial intraindividual Doppler measurements of cardiovascular function are feasible in wild-type and genetically altered mice before and after microsurgical procedures or to follow age-related changes. Fifty-megahertz ultrasound biomicroscopy allows to record the first beats of the embryonic mouse heart at somite stage 5, and the first Doppler-flow signals can be recorded after the onset of intrauterine cardiovascular function at somite stage 7. Using 10- to 20-MHz ultrasound transducers in the mouse embryo, cardiac, and circulatory function can be studied as early as 7.5 days after postcoital mucous plug. Postnatal Doppler ultrasound examinations in mice are possible from birth to senescent age. Several strain-, age-, and gender-related differences of Doppler ultrasound findings have been reported in mice. Results of Doppler examinations are influenced by the experimental settings as stress testing or different forms of anesthesia. This review summarizes the present status of Doppler ultrasound examinations in mice and animal handling in the framework of a comprehensive phenotype characterization of cardiac contractile and circulatory function.

PMID: 17214632

2006

Molecular models for murine sperm-egg binding

J Biol Chem. 2006 May 19;281(20):13853-6. Epub 2006 Feb 1. Clark GF, Dell A.

Department of Obstetrics, Gynecology and Women's Health, Division of Reproductive and Perinatal Research, School of Medicine, University of Missouri, Columbia, Missouri 65202, USA. clarkgf@health.missouri.edu Abstract Murine sperm initiate fertilization by binding to the specialized extracellular matrix of mouse eggs, known as the zona pellucida. Over the past decade, powerful genetic, biophysical, and biochemical techniques have been employed to gain new insights into this interaction. Evidence from these studies does not support either of two major models for binding first proposed over two decades ago. Two more recently established models suggest that protein-protein interactions predominate during this initial stage of fertilization. Another model proposes that about 75-80% of the murine sperm bound to zona pellucida under well defined in vitro conditions is carbohydrate dependent, with the remaining sperm bound via protein-protein interactions. Mounting evidence suggests that the carbohydrate sequences coating the murine egg could be employed as specific immune recognition markers. Continued investigation of this system may resolve many of these controversial findings and reveal novel functions for murine zona pellucida glycoproteins.

PMID: 16455664


http://www.ncbi.nlm.nih.gov/pubmed/18651668

2003

The postnatal development of the hypothalamic-pituitary-adrenal axis in the mouse

Int J Dev Neurosci. 2003 May;21(3):125-32.

Schmidt MV, Enthoven L, van der Mark M, Levine S, de Kloet ER, Oitzl MS. Source Gorlaeus Laboratories, Division of Medical Pharmacology, Leiden/Amsterdam Center for Drug Research, Leiden University Medical Centre, Leiden University, P.O. Box 9502, The Netherlands. m.schmidt@lacdr.leidenuniv.nl Erratum in Int J Dev Neurosci. 2006 Jun;24(4):293. Schmidt, M [corrected to Schmidt, Mathias V].

Abstract

The main characteristic of the postnatal development of the stress system in the rat is the so-called stress hypo-responsive period (SHRP). Lasting from postnatal day (pnd) 4 to pnd 14, this period is characterized by very low basal corticosterone levels and an inability of mild stressors to induce an enhanced ACTH and corticosterone release. During the last years, the mouse has become a generally used animal in stress research, also due to the wide availability of genetically modified mouse strains. However, very few data are available on the ontogeny of the stress system in the mouse. This study therefore describes the postnatal ontogeny of peripheral and central aspects of the hypothalamic-pituitary-adrenal (HPA) axis in the mouse. We measured ACTH and corticosterone in blood and CRH, urocortin 3 (UCN3), mineralocorticoid receptor (MR) and glucocorticoid receptor (GR) transcripts in the brain at postnatal days 1, 2, 4, 6, 9, 12, 14 and 16. Our results show that we can subdivide the postnatal development of the HPA axis in the mouse in two phases. The first phase corresponds to the SHRP in the rat and lasts from right after birth (pnd 1) until pnd 12. Basal corticosterone levels were low and novelty exposure did not enhance corticosterone or ACTH levels. This period is further characterized by a high expression of CRH in the paraventricular nucleus (PVN) of the hypothalamus. Expression levels of GR in the hippocampus and UCN3 in the perifornical area are low at birth but increase significantly during the SHRP, both reaching the highest expression level at pnd 12. In the second phase, the mice have developed past the SHRP and were now exhibiting enhanced corticosterone basal levels and a response of ACTH and corticosterone to mild novelty stress. CRH expression was decreased significantly, while expression of UCN3 and GR remained high, with a small decrease at pnd 16. The expression of MR in the hippocampus was very dynamic throughout the postnatal development of the HPA axis and changed in a time and subregion specific manner. These results demonstrate for the first time the correlation between the postnatal endocrine development of the mouse and gene expression changes of central regulators of HPA axis function.

PMID 12711350

mouse oocyte survival

Taste Development

http://www.pnas.org/content/104/7/2253.long

"Taste tissue development in mice starts around embryonic day (E) 11.5, after the emergence of the tongue swelling on the floor of developing mandible (8, 9). This is followed by formation of the taste placode (E12.5), gustatory papillae (E13.5), and taste buds (around birth)"


1987

Neurulation in the mouse. I. The ontogenesis of neural segments and the determination of topographical regions in a central nervous system

Sakai Y. Anat Rec. 1987 Aug;218(4):450-7.PMID: 3662046

"Ontogenesis of neural segments and positional relationships between the segments and other organs during neurulation were studied in 1,423 ICR mouse embryos by binocular dissecting, light, and scanning electron microscopy. Late in the presomite stage, two transverse sulci, preotic and otic, were seen on the prospective luminal surface of the neural folds. By somite stage 19, the former subdivided into five neuromeres, and by somite stage 21, the latter subdivided into four neuromeres. From the rostral, preotic sulcus, moreover, five other neuromeres were formed by somite stage 20, and between the otic sulcus and the first somite, two neuromeres were formed by somite stage 28. In the caudal part, from the level of the first somite, a total of 39 neuromeres were formed one after another by somite stage 39, and their positions almost correlated with each corresponding somite. Furthermore, the isthmus grew in the boundary between the fifth and sixth neuromere. The most protruding zone in the preotic sulcus formed the eighth neuromere and was located adjacent to the first branchial arch and the trigeminal ganglion. The most protruding zone in the otic sulcus also formed the 11th neuromere and was located adjacent to the second branchial arch. The 12th and 13th neuromeres were situated adjacent to the otic vesicle; the 23rd to 28th neuromeres, adjacent to the forelimb bud; and the 40th to 46th neuromeres, adjacent to the hindlimb bud."

The histogenetic potential of neural plate cells of early-somite-stage mouse embryos

Chan WY, Tam PP. J Embryol Exp Morphol. 1986 Jul;96:183-93.PMID: 3805982

Background Reading

"Postnatal fast muscle fibre type growth is divided into distinct phases in mouse xtensor digitorum longus (EDL): myofibre hypertrophy is initially supported by a rapid increase in the number of myonuclei, but nuclear addition stops around P21. Since the significant myofibre hypertrophy from P21 to adulthood occurs without the net addition of new myonuclei, a considerable expansion of the myonuclear domain results. Satellite cell numbers are initially stable, but then decrease to reach the adult level by P21. Thus the adult number of both myonuclei and satellite cells is already established by three weeks of postnatal growth in mouse." (EDL fast type II fibres in adult)
  • Mouse models of congenital cardiovascular disease. Moon A. Curr Top Dev Biol. 2008;84:171-248. Review. PMID: 19186245
  • Mouse models for investigating the developmental basis of human birth defects. Moon AM. Pediatr Res. 2006 Jun;59(6):749-55. Epub 2006 Apr 26. PMID: 16641221


  • http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=18083227 murine placenta contains two invasive cell types, trophoblast giant cells (TGC) and glycogen trophoblast cells (GlyT) TGC population is now recognized to have several subtypes, two of which are invasive; TGCs that form a barrier between the maternal decidua and the underlying placenta (parietal TGCs) and TGCs that invade via an endovascular route (spiral artery-associated TGCs)

A 4D atlas and morphologic database

<pubmed>18713865</pubmed>| PMC2527911 | PNAS "This work makes magnetic resonance microscopy of the mouse embryo and neonate broadly available with carefully annotated normative data and an extensive environment for collaborations."

4D Atlas and Morphologic Database


Mouse Lung Stage Ages

mouse lung development can be divided into 5 stages:

  1. Embryonic Stage (E9 to E11.5), in which lung buds originate as an outgrowth from the ventral wall of the foregut where lobar division occurs
  2. Pseudoglandular Stage (E11.5 to E16.5), in which conducting epithelial tubes surrounded by thick mesenchyme are formed, distinguished by extensive airway branching
  3. Canalicular stage (E16.5 to E17.5), in which bronchioles are produced, characterized by an increasing number of capillaries in close contact with cuboidal epithelium and the beginning of alveolar epithelium development
  4. Saccular Stage (E17.5 to PN5), in which alveolar ducts and air sacs are developed
  5. Alveolar Stage (PN5 to PN28), in which secondary septation occurs, defined by a marked increase of the number and size of capillaries and alveoli

PMID 17237346

Mouse

Stage Age Features
Embryonic E9 to E11.5 lung buds originate as an outgrowth from the ventral wall of the foregut where lobar division occurs
Pseudoglandular E11.5 to E16.5 conducting epithelial tubes surrounded by thick mesenchyme are formed, extensive airway branching
Canalicular E16.5 to E17.5 bronchioles are produced, increasing number of capillaries in close contact with cuboidal epithelium and the beginning of alveolar epithelium development
Saccular E17.5 to PN5 alveolar ducts and air sacs are developed
Alveolar PN5 to PN28 secondary septation occurs, marked increase of the number and size of capillaries and alveoli

Human + Mouse

Stage Human Mouse Features
Embryonic week 4 to 5 E9 to E11.5 lung buds originate as an outgrowth from the ventral wall of the foregut where lobar division occurs
Pseudoglandular week 5 to 17 E11.5 to E16.5 conducting epithelial tubes surrounded by thick mesenchyme are formed, extensive airway branching
Canalicular week 16 to 25 E16.5 to E17.5 bronchioles are produced, increasing number of capillaries in close contact with cuboidal epithelium and the beginning of alveolar epithelium development
Saccular week 24 to 40 E17.5 to PN5 alveolar ducts and air sacs are developed
Alveolar late fetal to 8 years PN5 to PN28 secondary septation occurs, marked increase of the number and size of capillaries and alveoli


Mouse Rat Comparison

Anatomical and Biological Date for the Mouse, Mus musculus, and Rat, Rattus norvegicus

                     Mouse            Rat

Weight 20-40 grams 300-4-- grams Adult male 25-90 grams 250-300 grams female Birth 1.5 grams 5-6 grams Life span 2 years - max. 2-3 years - Max 4 years Breeding age 3 yr. 2 mo. and weight male 60 days - 20-35 100 days - 300 grams

               grams  

female 50-60 days - 100 days - 200 grams

               Avg. 19   

Estrus cycle 4-5 days 5 days Gestation 17-21 days - 20-23 days - Avg. 21

               Avy. 19

with lactation Add 3-5 days Add 5-7 days Litter size 1-23 - Avg. 10-12 8-17 - Avg. 10 Number of 6-10 8-12 litters Weaning age and weight 16-20 days - 21 days - 40-50 grams

              10-12 grams 

Postpartum yes yes heart Breeding life male 18 months 12-14 months female 10-12 months, 6-10 1 year - 4-5 litters

              litters

Mating pair yes yes colony 1 male to 3 females 1 male to 3-4 females Water 1.5 cc/10 G. body wt. 1 cc/10 G.body wt.

              Ad libitum            Ad libitum

Feed usage 4-5 grams/day 12-15 grams/day Dry food consumption by young begins 10 days Approx. 12 days Hair growth 2-3 days 3-5 days apparent Recommended 72 F 70-80 F temperature Humidity 45-55 45-55 Light Minimal 14 2 hours Noise Minimal Minimal Heart beat adult 600 (328-780)min 328(261-600)min newborn ----- 161(81-241)min Breathing rate 163(84-230)min 94(75-115)min Body 97.5 F.(36.5C.) 99.1F.(37.3C) temperature Hematology RBC/mm 9 x 10 6 7-10x106 Avg.9.35x106 WBC/mm 8 - 16 x 103 6 - 18x103 Avg.9x103 Differential Lymphocytes 70# 78# Neutrophils 20# 20# Monocytes 10# <1% Ecsinophils <1% 2#

Data: http://netvet.wustl.edu/species/guinea/gpmodel.txt