Talk:Mouse Development: Difference between revisions
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Eurexpress transcriptome atlas http://www.eurexpress.org | Eurexpress transcriptome atlas http://www.eurexpress.org | ||
==2010== | |||
===Making the blastocyst: lessons from the mouse=== | |||
J Clin Invest. 2010 Apr;120(4):995-1003. doi: 10.1172/JCI41229. Epub 2010 Apr 1. | |||
Cockburn K, Rossant J. | |||
Source | |||
Department of Molecular Genetics, University of Toronto, Canada. | |||
Abstract | |||
Mammalian preimplantation development, which is the period extending from fertilization to implantation, results in the formation of a blastocyst with three distinct cell lineages. Only one of these lineages, the epiblast, contributes to the embryo itself, while the other two lineages, the trophectoderm and the primitive endoderm, become extra-embryonic tissues. Significant gains have been made in our understanding of the major events of mouse preimplantation development, and recent discoveries have shed new light on the establishment of the three blastocyst lineages. What is less clear, however, is how closely human preimplantation development mimics that in the mouse. A greater understanding of the similarities and differences between mouse and human preimplantation development has implications for improving assisted reproductive technologies and for deriving human embryonic stem cells. | |||
[http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2846056/figure/F1 Image - Stages of mouse and human preimplantation development] | |||
PMID 20364097 | |||
==2008== | ==2008== | ||
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Postnatal lung development is not well characterized in mice, especially the time point when alveolarization is completed. Using the total length and the length density of the free septal edge as measured for the formation of new septa, we followed alveolarization throughout postnatal lung development (days 2-125). Furthermore, the alveolar surface area was estimated. The formation of new septa was observed until day 36. Approximately 10% of the septa present in adult mice were formed prenatally by branching morphogenesis, approximately 50% were generated postnatally before and approximately 40% after maturation of the alveolar microvasculature. Approximately 5% of the alveolar surface area present during adulthood was present before alveolarization started, approximately 55% was formed during alveolarization (days 4-36) and approximately 40% afterward due to growth processes. We conclude that alveolarization continues until young adulthood and that the maturation of the alveolar microvasculature does not preclude further alveolarization. | Postnatal lung development is not well characterized in mice, especially the time point when alveolarization is completed. Using the total length and the length density of the free septal edge as measured for the formation of new septa, we followed alveolarization throughout postnatal lung development (days 2-125). Furthermore, the alveolar surface area was estimated. The formation of new septa was observed until day 36. Approximately 10% of the septa present in adult mice were formed prenatally by branching morphogenesis, approximately 50% were generated postnatally before and approximately 40% after maturation of the alveolar microvasculature. Approximately 5% of the alveolar surface area present during adulthood was present before alveolarization started, approximately 55% was formed during alveolarization (days 4-36) and approximately 40% afterward due to growth processes. We conclude that alveolarization continues until young adulthood and that the maturation of the alveolar microvasculature does not preclude further alveolarization. | ||
PMID | PMID 18651668 | ||
===In vivo quantification of embryonic and placental growth during gestation in mice using micro-ultrasound=== | ===In vivo quantification of embryonic and placental growth during gestation in mice using micro-ultrasound=== | ||
Revision as of 16:41, 16 June 2012
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Glossary Links
Cite this page: Hill, M.A. (2024, April 16) Embryology Mouse Development. Retrieved from https://embryology.med.unsw.edu.au/embryology/index.php/Talk:Mouse_Development |
original page | Mouse Stages | Mouse Timeline (day by day) | Mouse Detailed Timeline (day by day) | Mouse Estrous Cycle | Mouse Heart
- Video - Dissection of 6.5 dpc Mouse Embryos
- GenitoUrinary Development Molecular Anatomy Project (GUDMAP) Renal Development Tutorial | Genital Development Tutorial
2012
Application of in utero electroporation and live imaging in the analyses of neuronal migration during mouse brain development
Med Mol Morphol. 2012 Dec;45(1):1-6. Epub 2012 Mar 20.
Nishimura YV, Shinoda T, Inaguma Y, Ito H, Nagata K. Source Department of Molecular Neurobiology, Institute for Developmental Research, Aichi Human Service Center, 713-8 Kamiya, Kasugai, Aichi, 480-0392, Japan.
Abstract
Correct neuronal migration is crucial for brain architecture and function. During cerebral cortex development (corticogenesis), excitatory neurons generated in the proliferative zone of the dorsal telencephalon (mainly ventricular zone) move through the intermediate zone and migrate past the neurons previously located in the cortical plate and come to rest just beneath the marginal zone. The in utero electroporation technique is a powerful method for rapid gain- and loss-of-function studies of neuronal development, especially neuronal migration. This method enabled us to introduce genes of interest into ventricular zone progenitor cells of mouse embryos and to observe resulting phenotypes such as proliferation, migration, and cell morphology at later stages. In this Award Lecture Review, we focus on the application of the in utero electroporation method to functional analyses of cytoskeleton-related protein septin. We then refer to, as an advanced technique, the in utero electroporation-based real-time imaging method for analyses of cell signaling regulating neuronal migration. The in utero electroporation method and its application would contribute to medical molecular morphology through identification and characterization of the signaling pathways disorganized in various neurological and psychiatric disorders.
PMID 22431177
Cell fate decisions and axis determination in the early mouse embryo
Development. 2012 Jan;139(1):3-14.
Takaoka K, Hamada H. Source Developmental Genetics Group, Graduate School of Frontier Biosciences, Osaka University, 1-1 Yamada-oka, Suita, Osaka 565-0871, Japan.
Abstract
The mouse embryo generates multiple cell lineages, as well as its future body axes in the early phase of its development. The early cell fate decisions lead to the generation of three lineages in the pre-implantation embryo: the epiblast, the primitive endoderm and the trophectoderm. Shortly after implantation, the anterior-posterior axis is firmly established. Recent studies have provided a better understanding of how the earliest cell fate decisions are regulated in the pre-implantation embryo, and how and when the body axes are established in the pregastrulation embryo. In this review, we address the timing of the first cell fate decisions and of the establishment of embryonic polarity, and we ask how far back one can trace their origins.
PMID 22147950
2011
A conditional knockout resource for the genome-wide study of mouse gene function
Nature. 2011 Jun 15;474(7351):337-42. doi: 10.1038/nature10163.
Skarnes WC, Rosen B, West AP, Koutsourakis M, Bushell W, Iyer V, Mujica AO, Thomas M, Harrow J, Cox T, Jackson D, Severin J, Biggs P, Fu J, Nefedov M, de Jong PJ, Stewart AF, Bradley A. Source Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1SA, UK. skarnes@sanger.ac.uk
Abstract
Gene targeting in embryonic stem cells has become the principal technology for manipulation of the mouse genome, offering unrivalled accuracy in allele design and access to conditional mutagenesis. To bring these advantages to the wider research community, large-scale mouse knockout programmes are producing a permanent resource of targeted mutations in all protein-coding genes. Here we report the establishment of a high-throughput gene-targeting pipeline for the generation of reporter-tagged, conditional alleles. Computational allele design, 96-well modular vector construction and high-efficiency gene-targeting strategies have been combined to mutate genes on an unprecedented scale. So far, more than 12,000 vectors and 9,000 conditional targeted alleles have been produced in highly germline-competent C57BL/6N embryonic stem cells. High-throughput genome engineering highlighted by this study is broadly applicable to rat and human stem cells and provides a foundation for future genome-wide efforts aimed at deciphering the function of all genes encoded by the mammalian genome.
PMID: 21677750 http://www.ncbi.nlm.nih.gov/pubmed/21677750
http://www.nature.com/nature/journal/v474/n7351/full/nature10163.html
A high-resolution anatomical atlas of the transcriptome in the mouse embryo
PLoS Biol. 2011 Jan 18;9(1):e1000582.
Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, Magen A, Canidio E, Pagani M, Peluso I, Lin-Marq N, Koch M, Bilio M, Cantiello I, Verde R, De Masi C, Bianchi SA, Cicchini J, Perroud E, Mehmeti S, Dagand E, Schrinner S, Nürnberger A, Schmidt K, Metz K, Zwingmann C, Brieske N, Springer C, Hernandez AM, Herzog S, Grabbe F, Sieverding C, Fischer B, Schrader K, Brockmeyer M, Dettmer S, Helbig C, Alunni V, Battaini MA, Mura C, Henrichsen CN, Garcia-Lopez R, Echevarria D, Puelles E, Garcia-Calero E, Kruse S, Uhr M, Kauck C, Feng G, Milyaev N, Ong CK, Kumar L, Lam M, Semple CA, Gyenesei A, Mundlos S, Radelof U, Lehrach H, Sarmientos P, Reymond A, Davidson DR, Dollé P, Antonarakis SE, Yaspo ML, Martinez S, Baldock RA, Eichele G, Ballabio A.
Source
Telethon Institute of Genetics and Medicine, Naples, Italy.
Abstract
Ascertaining when and where genes are expressed is of crucial importance to understanding or predicting the physiological role of genes and proteins and how they interact to form the complex networks that underlie organ development and function. It is, therefore, crucial to determine on a genome-wide level, the spatio-temporal gene expression profiles at cellular resolution. This information is provided by colorimetric RNA in situ hybridization that can elucidate expression of genes in their native context and does so at cellular resolution. We generated what is to our knowledge the first genome-wide transcriptome atlas by RNA in situ hybridization of an entire mammalian organism, the developing mouse at embryonic day 14.5. This digital transcriptome atlas, the Eurexpress atlas (http://www.eurexpress.org), consists of a searchable database of annotated images that can be interactively viewed. We generated anatomy-based expression profiles for over 18,000 coding genes and over 400 microRNAs. We identified 1,002 tissue-specific genes that are a source of novel tissue-specific markers for 37 different anatomical structures. The quality and the resolution of the data revealed novel molecular domains for several developing structures, such as the telencephalon, a novel organization for the hypothalamus, and insight on the Wnt network involved in renal epithelial differentiation during kidney development. The digital transcriptome atlas is a powerful resource to determine co-expression of genes, to identify cell populations and lineages, and to identify functional associations between genes relevant to development and disease.
PMID: 21267068 http://www.ncbi.nlm.nih.gov/pubmed/21267068
http://www.plosbiology.org/article/info%3Adoi%2F10.1371%2Fjournal.pbio.1000582
Eurexpress transcriptome atlas http://www.eurexpress.org
2010
Making the blastocyst: lessons from the mouse
J Clin Invest. 2010 Apr;120(4):995-1003. doi: 10.1172/JCI41229. Epub 2010 Apr 1.
Cockburn K, Rossant J. Source Department of Molecular Genetics, University of Toronto, Canada.
Abstract
Mammalian preimplantation development, which is the period extending from fertilization to implantation, results in the formation of a blastocyst with three distinct cell lineages. Only one of these lineages, the epiblast, contributes to the embryo itself, while the other two lineages, the trophectoderm and the primitive endoderm, become extra-embryonic tissues. Significant gains have been made in our understanding of the major events of mouse preimplantation development, and recent discoveries have shed new light on the establishment of the three blastocyst lineages. What is less clear, however, is how closely human preimplantation development mimics that in the mouse. A greater understanding of the similarities and differences between mouse and human preimplantation development has implications for improving assisted reproductive technologies and for deriving human embryonic stem cells.
Image - Stages of mouse and human preimplantation development
PMID 20364097
2008
Developmental alveolarization of the mouse lung.
Dev Dyn. 2008 Aug;237(8):2108-16.
Mund SI, Stampanoni M, Schittny JC.
Institute of Anatomy, University of Bern, Switzerland.
Abstract Postnatal lung development is not well characterized in mice, especially the time point when alveolarization is completed. Using the total length and the length density of the free septal edge as measured for the formation of new septa, we followed alveolarization throughout postnatal lung development (days 2-125). Furthermore, the alveolar surface area was estimated. The formation of new septa was observed until day 36. Approximately 10% of the septa present in adult mice were formed prenatally by branching morphogenesis, approximately 50% were generated postnatally before and approximately 40% after maturation of the alveolar microvasculature. Approximately 5% of the alveolar surface area present during adulthood was present before alveolarization started, approximately 55% was formed during alveolarization (days 4-36) and approximately 40% afterward due to growth processes. We conclude that alveolarization continues until young adulthood and that the maturation of the alveolar microvasculature does not preclude further alveolarization.
PMID 18651668
In vivo quantification of embryonic and placental growth during gestation in mice using micro-ultrasound
Mu J, Slevin JC, Qu D, McCormick S, Adamson SL. Reprod Biol Endocrinol. 2008 Aug 12;6:34. PMID: 18700008
- "RESULTS: Gestational sac dimension provided the earliest measure of conceptus size. Sac dimension derived using regression analysis increased from 0.84 mm at E7.5 to 6.44 mm at E11.5 when it was discontinued. The earliest measurement of embryo size was crown-rump length (CRL) which increased from 1.88 mm at E8.5 to 16.22 mm at E16.5 after which it exceeded the field of view. From E10.5 to E18.5 (full term), progressive increases were observed in embryonic biparietal diameter (BPD) (0.79 mm to 7.55 mm at E18.5), abdominal circumference (AC) (4.91 mm to 26.56 mm), and eye lens diameter (0.20 mm to 0.93 mm). Ossified femur length was measureable from E15.5 (1.06 mm) and increased linearly to 2.23 mm at E18.5. In contrast, placental diameter (PD) and placental thickness (PT) increased from E10.5 to E14.5 then remained constant to term in accord with placental weight. Ultrasound and light microscopy measurements agreed with no significant bias and a discrepancy of less than 25%. Regression equations predicting gestational age from individual variables, and embryonic weight (BW) from CRL, BPD, and AC were obtained. The prediction equation BW = -0.757 + 0.0453 (CRL) + 0.0334 (AC) derived from CD-1 data predicted embryonic weights at E17.5 in three other strains of mice with a mean discrepancy of less than 16%. "
Synaptogenesis in the mouse olfactory bulb during glomerulus development
Eur J Neurosci. 2008 Jun;27(11):2838-46.
Blanchart A, Romaguera M, García-Verdugo JM, de Carlos JA, López-Mascaraque L.
Department of Cellular, Molecular and Developmental Neurobiology, Instituto Cajal, CSIC, Madrid, Spain.
Synaptogenesis is essential for the development of neuronal networks in the brain. In the olfactory bulb (OB) glomeruli, numerous synapses must form between sensory olfactory neurons and the dendrites of mitral/tufted and periglomerular cells. Glomeruli develop from E13 to E16 in the mouse, coincident with an increment of the neuropil in the border between the external plexiform (EPL) and olfactory nerve layers (ONL), coupled to an extensive labelling of phalloidin and GAP-43 from the ONL to EPL. We have tracked synaptogenesis in the OB during this period by electron microscopy (EM) and immunolabelling of the transmembrane synaptic vesicle glycoprotein SV-2. No SV-2 labelling or synapses were detected at E13, although electrodense junctions lacking synaptic vesicles could be observed by EM. At E14, sparse SV-2 labelling appears in the most ventral and medial part of the incipient OB, which displays a ventro-dorsal gradient by E15 but covers the entire OB by E16. These data establish a spatio-temporal pattern of synaptogenesis, which perfectly matches with the glomeruli formation in developing OB.
PMID: 18588529
2007
Doppler ultrasound in mice
Echocardiography. 2007 Jan;24(1):97-112.
Stypmann J.
Department of Cardiology and Angiology, Hospital of the University of Münster, Germany. stypmann@mednet.uni-muenster.de Abstract Color, power, spectral, and tissue Doppler have been applied to mice. Due to the noninvasive nature of the technique, serial intraindividual Doppler measurements of cardiovascular function are feasible in wild-type and genetically altered mice before and after microsurgical procedures or to follow age-related changes. Fifty-megahertz ultrasound biomicroscopy allows to record the first beats of the embryonic mouse heart at somite stage 5, and the first Doppler-flow signals can be recorded after the onset of intrauterine cardiovascular function at somite stage 7. Using 10- to 20-MHz ultrasound transducers in the mouse embryo, cardiac, and circulatory function can be studied as early as 7.5 days after postcoital mucous plug. Postnatal Doppler ultrasound examinations in mice are possible from birth to senescent age. Several strain-, age-, and gender-related differences of Doppler ultrasound findings have been reported in mice. Results of Doppler examinations are influenced by the experimental settings as stress testing or different forms of anesthesia. This review summarizes the present status of Doppler ultrasound examinations in mice and animal handling in the framework of a comprehensive phenotype characterization of cardiac contractile and circulatory function.
PMID: 17214632
2006
Molecular models for murine sperm-egg binding
J Biol Chem. 2006 May 19;281(20):13853-6. Epub 2006 Feb 1. Clark GF, Dell A.
Department of Obstetrics, Gynecology and Women's Health, Division of Reproductive and Perinatal Research, School of Medicine, University of Missouri, Columbia, Missouri 65202, USA. clarkgf@health.missouri.edu Abstract Murine sperm initiate fertilization by binding to the specialized extracellular matrix of mouse eggs, known as the zona pellucida. Over the past decade, powerful genetic, biophysical, and biochemical techniques have been employed to gain new insights into this interaction. Evidence from these studies does not support either of two major models for binding first proposed over two decades ago. Two more recently established models suggest that protein-protein interactions predominate during this initial stage of fertilization. Another model proposes that about 75-80% of the murine sperm bound to zona pellucida under well defined in vitro conditions is carbohydrate dependent, with the remaining sperm bound via protein-protein interactions. Mounting evidence suggests that the carbohydrate sequences coating the murine egg could be employed as specific immune recognition markers. Continued investigation of this system may resolve many of these controversial findings and reveal novel functions for murine zona pellucida glycoproteins.
PMID: 16455664
http://www.ncbi.nlm.nih.gov/pubmed/18651668
mouse oocyte survival
- Intact fetal ovarian cord formation promotes mouse oocyte survival and development http://www.ncbi.nlm.nih.gov/pubmed/20064216 | http://www.biomedcentral.com/1471-213X/10/2
Taste Development
http://www.pnas.org/content/104/7/2253.long
- "Taste tissue development in mice starts around embryonic day (E) 11.5, after the emergence of the tongue swelling on the floor of developing mandible (8, 9). This is followed by formation of the taste placode (E12.5), gustatory papillae (E13.5), and taste buds (around birth)"
1987
Neurulation in the mouse. I. The ontogenesis of neural segments and the determination of topographical regions in a central nervous system
Sakai Y. Anat Rec. 1987 Aug;218(4):450-7.PMID: 3662046
- "Ontogenesis of neural segments and positional relationships between the segments and other organs during neurulation were studied in 1,423 ICR mouse embryos by binocular dissecting, light, and scanning electron microscopy. Late in the presomite stage, two transverse sulci, preotic and otic, were seen on the prospective luminal surface of the neural folds. By somite stage 19, the former subdivided into five neuromeres, and by somite stage 21, the latter subdivided into four neuromeres. From the rostral, preotic sulcus, moreover, five other neuromeres were formed by somite stage 20, and between the otic sulcus and the first somite, two neuromeres were formed by somite stage 28. In the caudal part, from the level of the first somite, a total of 39 neuromeres were formed one after another by somite stage 39, and their positions almost correlated with each corresponding somite. Furthermore, the isthmus grew in the boundary between the fifth and sixth neuromere. The most protruding zone in the preotic sulcus formed the eighth neuromere and was located adjacent to the first branchial arch and the trigeminal ganglion. The most protruding zone in the otic sulcus also formed the 11th neuromere and was located adjacent to the second branchial arch. The 12th and 13th neuromeres were situated adjacent to the otic vesicle; the 23rd to 28th neuromeres, adjacent to the forelimb bud; and the 40th to 46th neuromeres, adjacent to the hindlimb bud."
The histogenetic potential of neural plate cells of early-somite-stage mouse embryos
Chan WY, Tam PP. J Embryol Exp Morphol. 1986 Jul;96:183-93.PMID: 3805982
Background Reading
- "Postnatal fast muscle fibre type growth is divided into distinct phases in mouse xtensor digitorum longus (EDL): myofibre hypertrophy is initially supported by a rapid increase in the number of myonuclei, but nuclear addition stops around P21. Since the significant myofibre hypertrophy from P21 to adulthood occurs without the net addition of new myonuclei, a considerable expansion of the myonuclear domain results. Satellite cell numbers are initially stable, but then decrease to reach the adult level by P21. Thus the adult number of both myonuclei and satellite cells is already established by three weeks of postnatal growth in mouse." (EDL fast type II fibres in adult)
- Mouse models of congenital cardiovascular disease. Moon A. Curr Top Dev Biol. 2008;84:171-248. Review. PMID: 19186245
- Mouse models for investigating the developmental basis of human birth defects. Moon AM. Pediatr Res. 2006 Jun;59(6):749-55. Epub 2006 Apr 26. PMID: 16641221
- http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=18083227 murine placenta contains two invasive cell types, trophoblast giant cells (TGC) and glycogen trophoblast cells (GlyT) TGC population is now recognized to have several subtypes, two of which are invasive; TGCs that form a barrier between the maternal decidua and the underlying placenta (parietal TGCs) and TGCs that invade via an endovascular route (spiral artery-associated TGCs)
A 4D atlas and morphologic database
<pubmed>18713865</pubmed>| PMC2527911 | PNAS "This work makes magnetic resonance microscopy of the mouse embryo and neonate broadly available with carefully annotated normative data and an extensive environment for collaborations."
