Talk:Medaka Development

2010

Snail gene expression in the medaka, Oryzias latipes

Gene Expr Patterns. 2010 Nov 19. [Epub ahead of print] Liedtke D, Erhard I, Schartl M.

Physiological Chemistry I, University of Würzburg, Biozentrum, Am Hubland, Würzburg, Germany. Abstract Snail transcription factors have prominent roles during embryonic development of vertebrates. They are often involved in cell migration processes during neural crest development, epithelial-mesenchymal transition and cancer progression. Comparative expression studies of snai gene family members in different vertebrate species are expected to contribute to a better understanding of their roles during development and reflect their evolutionary history. To investigate and to compare the expression patterns of snai genes in a second main fish model we used the medaka fish (Oryzias latipes), a complementary teleost model to zebrafish. We identified three snai gene family members, snai1a, snai1b and snai2. Phylogenetic and synteny analysis show a close relatedness of all family members to other vertebrate snai genes. Surprisingly, no homologue of snai3 could be identified in medaka, although this gene is present in zebrafish and the pufferfishes. Here we demonstrate that while most expression domains of medaka snai genes are comparable to zebrafish, the contribution of the individual paralogs to the overall pattern differs between the two teleosts and indicate lineage specific expression shuffling.

Copyright © 2010. Published by Elsevier B.V. PMID: 21094700

The National BioResource Project Medaka (NBRP Medaka): an integrated bioresource for biological and biomedical sciences

Exp Anim. 2010;59(1):13-23.

Sasado T, Tanaka M, Kobayashi K, Sato T, Sakaizumi M, Naruse K.

Laboratory of Bioresources, National Institute for Basic Biology, Okazaki, Japan. Abstract Medaka (Oryzias latipes) is a small freshwater teleost fish that serves as a model vertebrate organism in various fields of biology including development, genetics, toxicology and evolution. The recent completion of the medaka genome sequencing project has promoted the use of medaka as a comparative and complementary material for research on other vertebrates such as zebrafish, sticklebacks, mice, and humans. The Japanese government has supported the development of Medaka Bioresources since 2002. The second term of the Medaka Bioresource Project started in 2007. The National Institute for Basic Biology and Niigata University were selected as the core organizations for this project. More than 400 strains including more than 300 spontaneous and induced mutants, 8 inbred lines, 21 transgenic lines, 20 medaka-related species and 66 wild stock lines of medaka are now being provided to the scientific community and educational non-profit organizations. In addition to these live fish, NBRP Medaka is also able to provide cDNA/EST clones such as full-length cDNA and BAC/fosmid clones covering 90% of the medaka genome. All these resources can be found on the NBRP Medaka website (http://shigen.lab.nig.ac.jp/medaka/), and users can order any resource using the shopping cart system. We believe these resources will facilitate the further use of medaka and help to promote new findings for this vertebrate species.

PMID: 20224166