Talk:Histology: Difference between revisions
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== Common | == Common histology stains == | ||
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Revision as of 17:48, 5 November 2009
Common histology stains
Stain | Common use | Nucleus | Cytoplasm | Red blood cell (RBC) | Collagen fibers | Specifically stains |
---|---|---|---|---|---|---|
Haematoxylin | General staining when paired with eosin | Blue | N/A | N/A | N/A | Nucleic acids—blue
Rough Endoplasmic Reticulum (RER) —blue |
Eosin | General staining when paired with haematoxylin | N/A | Pink | Orange/red | Pink | Elastic fibers—pink
Reticular fibers—pink |
Toluidine blue | General staining | Blue | Blue | Blue | Blue | Mast cells granules—purple |
Masson's trichrome stain | Connective tissue | Black | Red/pink | Red | Blue/green | Cartilage—blue/green
Muscle fibers—red |
Mallory's trichrome stain | Connective tissue | Red | Pale red | Orange | Deep blue | Keratin—orange
Cartilage—blue Bone matrix—deep blue Muscle fibers—red |
Weigert's elastic stain | Elastic fibers | Blue/black | N/A | N/A | N/A | Elastic fibers—blue/black |
Heidenhains'azan trichrome stain | Distinguishing cells from extracellular components | Red/purple | Pink | Red | Blue | Muscle fibers—red
Cartilage—blue Bone matrix—blue |
Silver stain | Reticular fibers, nerve fibers | N/A | N/A | N/A | Reticular fibers—brown/black
Nerve fibers—brown/black | |
Wright's stain | Blood cells | Bluish/purple | Bluish/gray | Red/pink | N/A | Neutrophil granules—purple/pink
Eosinophil granules—bright red/orange Basophil granules—deep purple/violet Platelet granules—red/purple |
Orcein stain | Elastic fibres | Deep blue or crazy red | N/A | Bright red | Pink | Elastic fibres—dark brown
Mast cells granules—purple Smooth muscle—light blue |
Periodic acid-Schiff stain (PAS) | Basement membrane, localizing carbohydrates | Blue | N/A | N/A | Pink | Glycogen and other carbohydrates—magenta |
Nissl method - neurons Golgi method - neurons
Based on: Michael H. Ross, Wojciech Pawlina, (2006). Histology: A Text and Atlas. Hagerstown, MD: Lippincott Williams & Wilkins.