Talk:Estrous Cycle: Difference between revisions
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==2005== | |||
===A quantitative method for assessing stages of the rat estrous cycle=== | |||
Biotech Histochem. 2005 Mar-Apr;80(2):79-87. | |||
Hubscher CH, Brooks DL, Johnson JR. | |||
Source | |||
Department of Anatomical Sciences & Neurobiology, University of Louisville, Louisville, Kentucky 40292, USA. chhubs01@louisville.edu | |||
Abstract | |||
The impact of gender and/or hormone variations on a wide variety of neural functions makes the choice between studying males or females (or both) of a given species difficult. Although female rats are widely used experimentally, few studies control for the stage of estrus. More detailed information about how to distinguish the various stages of the estrous cycle is needed. For the present study, vaginal smears were obtained once a day and stained using an adaptation of the Papanicolaou (PAP) procedure. Images are provided of unstained "wet" samples and the corresponding PAP stained smears illustrating the cellular profile for each stage of the cycle as well as post-ovariectomy. The different cell populations across the cycle were quantified and ratios determined to show trends between the predominant and other cell types in each stage of the estrous cycle. Both stained and unstained images and cell quantification data provide valuable guidelines for distinguishing the stages of the estrous cycle. | |||
PMID 16195173 | |||
==1997== | ==1997== | ||
Revision as of 12:45, 2 January 2013
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Cite this page: Hill, M.A. (2024, April 25) Embryology Estrous Cycle. Retrieved from https://embryology.med.unsw.edu.au/embryology/index.php/Talk:Estrous_Cycle |
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<pubmed>17342777</pubmed>
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<pubmed>15953629</pubmed>
2005
A quantitative method for assessing stages of the rat estrous cycle
Biotech Histochem. 2005 Mar-Apr;80(2):79-87.
Hubscher CH, Brooks DL, Johnson JR. Source Department of Anatomical Sciences & Neurobiology, University of Louisville, Louisville, Kentucky 40292, USA. chhubs01@louisville.edu Abstract The impact of gender and/or hormone variations on a wide variety of neural functions makes the choice between studying males or females (or both) of a given species difficult. Although female rats are widely used experimentally, few studies control for the stage of estrus. More detailed information about how to distinguish the various stages of the estrous cycle is needed. For the present study, vaginal smears were obtained once a day and stained using an adaptation of the Papanicolaou (PAP) procedure. Images are provided of unstained "wet" samples and the corresponding PAP stained smears illustrating the cellular profile for each stage of the cycle as well as post-ovariectomy. The different cell populations across the cycle were quantified and ratios determined to show trends between the predominant and other cell types in each stage of the estrous cycle. Both stained and unstained images and cell quantification data provide valuable guidelines for distinguishing the stages of the estrous cycle.
PMID 16195173
1997
<pubmed>9433700</pubmed>
1989
<pubmed>2678711</pubmed>
