Talk:Chicken Development: Difference between revisions
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===Lhx1 in the proximal region of the optic vesicle permits neural retina development in the chicken=== | |||
Biol Open. 2012 Nov 15;1(11):1083-93. doi: 10.1242/bio.20121396. Epub 2012 Aug 28. | Biol Open. 2012 Nov 15;1(11):1083-93. doi: 10.1242/bio.20121396. Epub 2012 Aug 28. | ||
Kawaue T, Okamoto M, Matsuyo A, Inoue J, Ueda Y, Tomonari S, Noji S, Ohuchi H. | Kawaue T, Okamoto M, Matsuyo A, Inoue J, Ueda Y, Tomonari S, Noji S, Ohuchi H. | ||
Source | Source | ||
Department of Life Systems, Institute of Technology and Science, The University of Tokushima Graduate School , 2-1 Minami-Josanjima-cho, Tokushima 770-8506 , Japan. | Department of Life Systems, Institute of Technology and Science, The University of Tokushima Graduate School , 2-1 Minami-Josanjima-cho, Tokushima 770-8506 , Japan. | ||
Abstract | Abstract | ||
How the eye forms has been one of the fundamental issues in developmental biology. The retinal anlage first appears as the optic vesicle (OV) evaginating from the forebrain. Subsequently, its distal portion invaginates to form the two-walled optic cup, which develops into the outer pigmented and inner neurosensory layers of the retina. Recent work has shown that this optic-cup morphogenesis proceeds as a self-organizing activity without any extrinsic molecules. However, intrinsic factors that regulate this process have not been elucidated. Here we show that a LIM-homeobox gene, Lhx1, normally expressed in the proximal region of the nascent OV, induces a second neurosensory retina formation from the outer pigmented retina when overexpressed in the chicken OV. Lhx2, another LIM-homeobox gene supposed to be involved in early OV formation, could not substitute this function of Lhx1, while Lhx5, closely related to Lhx1, could replace it. Conversely, knockdown of Lhx1 expression by RNA interference resulted in the formation of a small or pigmented vesicle. These results suggest that the proximal region demarcated by Lhx1 expression permits OV development, eventually dividing the two retinal domains. | How the eye forms has been one of the fundamental issues in developmental biology. The retinal anlage first appears as the optic vesicle (OV) evaginating from the forebrain. Subsequently, its distal portion invaginates to form the two-walled optic cup, which develops into the outer pigmented and inner neurosensory layers of the retina. Recent work has shown that this optic-cup morphogenesis proceeds as a self-organizing activity without any extrinsic molecules. However, intrinsic factors that regulate this process have not been elucidated. Here we show that a LIM-homeobox gene, Lhx1, normally expressed in the proximal region of the nascent OV, induces a second neurosensory retina formation from the outer pigmented retina when overexpressed in the chicken OV. Lhx2, another LIM-homeobox gene supposed to be involved in early OV formation, could not substitute this function of Lhx1, while Lhx5, closely related to Lhx1, could replace it. Conversely, knockdown of Lhx1 expression by RNA interference resulted in the formation of a small or pigmented vesicle. These results suggest that the proximal region demarcated by Lhx1 expression permits OV development, eventually dividing the two retinal domains. | ||
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Cite this page: Hill, M.A. (2024, April 18) Embryology Chicken Development. Retrieved from https://embryology.med.unsw.edu.au/embryology/index.php/Talk:Chicken_Development |
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Note - This sub-heading shows an automated computer PubMed search using the listed sub-heading term. References appear in this list based upon the date of the actual page viewing. Therefore the list of references do not reflect any editorial selection of material based on content or relevance. In comparison, references listed on the content page and discussion page (under the publication year sub-headings) do include editorial selection based upon relevance and availability. (More? Pubmed Most Recent)
Chicken embryology
<pubmed limit=5>Chicken embryology</pubmed>
Chicken development
<pubmed limit=5>Chicken development</pubmed>
2012
Chicken primordial germ cells use the anterior vitelline veins to enter the embryonic circulation
Biol Open. 2012 Nov 15;1(11):1146-52. doi: 10.1242/bio.20122592. Epub 2012 Sep 18.
De Melo Bernardo A, Sprenkels K, Rodrigues G, Noce T, Chuva De Sousa Lopes SM. Source Department of Anatomy and Embryology, Leiden University Medical Center , Einthovenweg 20, 2333 ZC Leiden , The Netherlands.
Abstract
During gastrulation, chicken primordial germ cells (PGCs) are present in an extraembryonic region of the embryo from where they migrate towards the genital ridges. This is also observed in mammals, but in chicken the vehicle used by the migratory PGCs is the vascular system. We have analysed the migratory pathway of chicken PGCs, focusing on the period of transition from the extraembryonic region to the intraembryonic vascular system.Our findings show that at Hamburger and Hamilton developmental stage HH12-HH14 the majority of PGCs concentrate axially in the sinus terminalis and favour transport axially via the anterior vitelline veins into the embryonic circulation. Moreover, directly blocking the blood flow through the anterior vitelline veins resulted in an accumulation of PGCs in the anterior region and a decreased number of PGCs in the genital ridges. We further confirmed the key role for the anterior vitelline veins in the correct migration of PGCs using an ex ovo culture method that resulted in defective morphogenetic development of the anterior vitelline veins.We propose a novel model for the migratory pathway of chicken PGCs whereby the anterior vitelline veins play a central role at the extraembryonic and embryonic interface. The chicken model of PGC migration through the vasculature may be a powerful tool to study the process of homing (inflammation and metastasis) due to the striking similarities in regulatory signaling pathways (SDF1-CXCR4) and the transient role of the vasculature.
PMID 23213395
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3507194
http://bio.biologists.org/content/1/11/1146
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial Share Alike License (http://creativecommons.org/licenses/by-nc-sa/3.0/).
Lhx1 in the proximal region of the optic vesicle permits neural retina development in the chicken
Biol Open. 2012 Nov 15;1(11):1083-93. doi: 10.1242/bio.20121396. Epub 2012 Aug 28.
Kawaue T, Okamoto M, Matsuyo A, Inoue J, Ueda Y, Tomonari S, Noji S, Ohuchi H. Source Department of Life Systems, Institute of Technology and Science, The University of Tokushima Graduate School , 2-1 Minami-Josanjima-cho, Tokushima 770-8506 , Japan.
Abstract
How the eye forms has been one of the fundamental issues in developmental biology. The retinal anlage first appears as the optic vesicle (OV) evaginating from the forebrain. Subsequently, its distal portion invaginates to form the two-walled optic cup, which develops into the outer pigmented and inner neurosensory layers of the retina. Recent work has shown that this optic-cup morphogenesis proceeds as a self-organizing activity without any extrinsic molecules. However, intrinsic factors that regulate this process have not been elucidated. Here we show that a LIM-homeobox gene, Lhx1, normally expressed in the proximal region of the nascent OV, induces a second neurosensory retina formation from the outer pigmented retina when overexpressed in the chicken OV. Lhx2, another LIM-homeobox gene supposed to be involved in early OV formation, could not substitute this function of Lhx1, while Lhx5, closely related to Lhx1, could replace it. Conversely, knockdown of Lhx1 expression by RNA interference resulted in the formation of a small or pigmented vesicle. These results suggest that the proximal region demarcated by Lhx1 expression permits OV development, eventually dividing the two retinal domains.
PMID 23213388
2011
4D fluorescent imaging of embryonic quail development
Cold Spring Harb Protoc. 2011 Nov 1;2011(11):1291-4. doi: 10.1101/pdb.top066613.
Canaria CA, Lansford R.
Abstract
Traditionally, our understanding of developmental biology has been based on the fixation and study of embryonic samples. Detailed microscopic scrutiny of static specimens at varying ages allowed for anatomical assessment of tissue development. The advent of confocal and two-photon excitation (2PE) microscopy enables researchers to acquire volumetric images in three dimensions (x, y, and z) plus time (t). Here, we present techniques for acquisition and analysis of three-dimensional (3D) time-lapse data. Both confocal microscopy and 2PE microscopy techniques are used. Data processing for tiled image stitching and time-lapse analysis is also discussed. The development of a transgenic Japanese quail system, as discussed here, has provided an embryonic model that is more easily accessible than mammalian models and more efficient to breed than the classic avian model, the chicken.
PMID 22046043
- Analysis of retinal cell development in chick embryo by immunohistochemistry and in ovo electroporation techniques http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=2822752&tool=pmcentrez&rendertype=abstract
