Talk:Cat Development: Difference between revisions
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PMID: 19262023 | PMID: 19262023 | ||
==2008== | |||
===Sex determining of cat embryo and some feline species=== | |||
Zygote. 2008 May;16(2):169-77. | |||
Ciani F, Cocchia N, Rizzo M, Ponzio P, Tortora G, Avallone L, Lorizio R. | |||
Source | |||
Department of Biological Structures, Functions and Technologies, University of Naples Federico II, Via F. Delpino, 1 - 80137 Naples, Italy. ciani@unina.it | |||
Abstract | |||
Sex identification in mammalian preimplantation embryos is a technique that is used currently for development of the embryo transfer industry for zootechnical animals and is, therefore, a resource for biodiversity preservation. The aim of the present study was to establish a rapid and reliable method for the sexing of preimplantation embryos in domestic cats. Here we describe the use of nested PCR identify Y chromosome-linked markers when starting from small amounts of DNA and test the method for the purpose of sexing different species of wild felids. To evaluate the efficiency of the primers, PCR analysis were performed first in blood samples of sex-known domestic cats. Cat embryos were produced both in vitro and in vivo and the blastocysts were biopsied. A Magnetic Resin System was used to capture a consistent amount of DNA from embryo biopsy and wild felid hairs. The results from nested PCR applied on cat blood that corresponded to the phenotypical sex. Nested PCR was also applied to 37 embryo biopsies and the final result was: 21 males and 16 females. Furthermore, beta-actin was amplified in each sample, as a positive control for DNA presence. Subsequently, nested PCR was performed on blood and hair samples from some wild felines and again the genotyping results and phenotype sex corresponded. The data show that this method is a rapid and repeatable option for sex determination in domestic cat embryos and some wild felids and that a small amount of cells is sufficient to obtain a reliable result. This technique, therefore, affords investigators a new approach that they can insert in the safeguard programmes of felida biodiversity. | |||
PMID 18405438 | |||
==2003== | ==2003== | ||
Revision as of 11:17, 4 November 2011
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Cite this page: Hill, M.A. (2024, April 16) Embryology Cat Development. Retrieved from https://embryology.med.unsw.edu.au/embryology/index.php/Talk:Cat_Development |
2009
Developmental changes of Müllerian and Wolffian ducts in domestic cat fetuses
Inomata T, Ninomiya H, Sakita K, Kashiwazaki N, Ito J, Ariga M, Inoue S. Exp Anim. 2009 Jan;58(1):41-5. PMID: 19151510
Development of external genitalia in fetal and neonatal domestic cats
J Vet Med Sci. 2009 Feb;71(2):139-45.
Inomata T, Ariga M, Sakita K, Kashiwazaki N, Ito J, Yokoh K, Ichikawa M, Ninomiya H, Inoue S.
Department of Laboratory Animal, School of Veterinary Medicine, Azabu University, Sagamihara, Kanagawa, Japan. inomata@azabu-u.ac.jp Abstract Development of the external genitalia of fetal and neonatal cat were studied macroscopically, paying attention to the formation of the labia and the sexual differentiation. The female urogenital folds budded from each side of the genital tubercle and, gradually extended to the tip of the genital tubercle by the 6.8 cm stage in crown-rump length. Then, the well-developed urogenital folds ensheathed completely the genital tubercle to form the prepuce of clitoris and the labia, flanking the external opening of vagina as the folds of skin which were equivalent to the labia minora in humans. The genital swellings known to become the labia majora in humans were clearly recognized in the caudolateral region of the genital tubercle during the fetal stage. These swellings became flat and obscure after birth. Thus, in cats the genital swellings did not join to the formation of the labia in the same way as in humans. The sex difference in the external genitalia was first observed at the 3.2-3.3 cm stages. In the male, the anogenital raphe appeared and the caudal portion of the genital swellings moved and fused each other at the caudal region of the genital tubercle. In the female, both features were not easy to observe.
PMID: 19262023
2008
Sex determining of cat embryo and some feline species
Zygote. 2008 May;16(2):169-77.
Ciani F, Cocchia N, Rizzo M, Ponzio P, Tortora G, Avallone L, Lorizio R. Source Department of Biological Structures, Functions and Technologies, University of Naples Federico II, Via F. Delpino, 1 - 80137 Naples, Italy. ciani@unina.it
Abstract
Sex identification in mammalian preimplantation embryos is a technique that is used currently for development of the embryo transfer industry for zootechnical animals and is, therefore, a resource for biodiversity preservation. The aim of the present study was to establish a rapid and reliable method for the sexing of preimplantation embryos in domestic cats. Here we describe the use of nested PCR identify Y chromosome-linked markers when starting from small amounts of DNA and test the method for the purpose of sexing different species of wild felids. To evaluate the efficiency of the primers, PCR analysis were performed first in blood samples of sex-known domestic cats. Cat embryos were produced both in vitro and in vivo and the blastocysts were biopsied. A Magnetic Resin System was used to capture a consistent amount of DNA from embryo biopsy and wild felid hairs. The results from nested PCR applied on cat blood that corresponded to the phenotypical sex. Nested PCR was also applied to 37 embryo biopsies and the final result was: 21 males and 16 females. Furthermore, beta-actin was amplified in each sample, as a positive control for DNA presence. Subsequently, nested PCR was performed on blood and hair samples from some wild felines and again the genotyping results and phenotype sex corresponded. The data show that this method is a rapid and repeatable option for sex determination in domestic cat embryos and some wild felids and that a small amount of cells is sufficient to obtain a reliable result. This technique, therefore, affords investigators a new approach that they can insert in the safeguard programmes of felida biodiversity.
PMID 18405438
2003
Testis morphometry, seminiferous epithelium cycle length, and daily sperm production in domestic cats (Felis catus)
Biol Reprod. 2003 May;68(5):1554-61. Epub 2002 Nov 27.
França LR, Godinho CL. Source Laboratory of Cellular Biology, Department of Morphology, Institute of Biological Sciences, Federal University of Minas Gerais, Belo Horizonte, MG, Brazil 31270-901. lrfranca@icb.ufmg.br
Abstract
There is very little information regarding the testis structure and function in domestic cats, mainly data related to the cycle of seminiferous epithelium and sperm production. The testis weight in cats investigated in the present study was 1.2 g. Compared with most mammalian species investigated, the value of 0.08% found for testes mass related to the body mass (gonadosomatic index) in cats is very low. The tunica albuginea volume density (%) in these animals was relatively high and comprised about 19% of the testis. Seminiferous tubule and Leydig cell volume density (%) in cats were approximately 90% and 6%, respectively. The mean tubular diameter was 220 microm, and 23 m of seminiferous tubule were found per testis and per gram of testis. The frequencies of the eight stages of the cycle, characterized according to the tubular morphology system, were as follows: stage 1, 24.9%; stage 2, 12.9%; stage 3, 7.7%; stage 4, 17.6%; stage 5, 7.2%; stage 6, 11.9%; stage 7, 6.8%; and stage 8, 11 %. The premeiotic and postmeiotic stage frequency was 46% and 37%, respectively. The duration of each cycle of seminiferous epithelium was 10.4 days and the total duration of spermatogenesis based on 4.5 cycles was 46.8 days. The number of round spermatids for each pachytene primary spermatocytes (meiotic index) was 2.8, meaning that significant cell loss (30%) occurred during the two meiotic divisions. The total number of germ cells and the number of round spermatids per each Sertoli cell nucleolus at stage 1 of the cycle were 9.8 and 5.1, respectively. The Leydig cell volume was approximately 2000 microm3 and the nucleus volume 260 microm3. Both Leydig and Sertoli cell numbers per gram of testis in cats were approximately 30 million. The daily sperm production per gram of testis in cats (efficiency of spermatogenesis) was approximately 16 million. To our knowledge, this is the first investigation to perform a more detailed and comprehensive study of the testis structure and function in domestic cats. Also, this is the first report in the literature showing Sertoli and Leydig cell number per gram of testis and the daily sperm production in any kind of feline species. In this regard, besides providing a background for comparative studies with other fields, the data obtained in the present work might be useful in future studies in which the domestic cat could be utilized as an appropriate receptor model for preservation of genetic stock from rare or endangered wild felines using the germ cell transplantation technique.
PMID: 12606460 http://www.ncbi.nlm.nih.gov/pubmed/12606460
