Talk:2018 Group Project 5
|Projects 2018: 1 Adrenal Medulla | 3 Melanocytes | 4 Cardiac | 5 Dorsal Root Ganglion|
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The project discussion page is where your group members can post discussion on the project topic. This will be demonstrated in the practical tutorial in week 3.
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Z5229399 (talk) 11:33, 14 August 2018 (AEST) Z5229597 (talk) 11:34, 14 August 2018 (AEST) Z5229431 (talk) 11:35, 14 August 2018 (AEST) Z5229549 (talk) 11:36, 14 August 2018 (AEST) Z5229438 (talk) 11:36, 21 August 2018 (AEST)
Peer Reviews (Lab 10)
Z5091101 (talk) 20:14, 3 October 2018 (AEST)z5091101Z5091101 (talk) 20:14, 3 October 2018 (AEST) There has been an extensive use of references which is great especially since this topic seems to be really complex. Maybe a few more images for the beginning part of the article will make it look more user-friendly. Definitely have a look over for any grammar/spelling issues.
Z5164785Embryonic origins has been well-written. Proof-read for typing errors. Neural crest migration section shows good research and use of terminology. Neuronal and glial development has nice concise information though it might be wise to add some more content. Also if the heading will be Glial dev, then neuron dev should be changed to Neuronal dev- for consistency. Adult function of ? However, this section is well-written! Concise and relevant- great work guys! Tissue Structure is starting to look good however needs more content. Really good student drawn image!! Though it might be good to be the image higher up on the page.
Molecular mechanisms/factors/genes has overall been well written. Perhaps a brief statement about what transcription factors are? Interesting image in abnormalities. I would personally appreciate an explanation of what I am seeing in the image. More discussion of a wider variety of abnormalities might be beneficial. Excellent coverage of animals models so far!! May be one more? Also, great use of images! Current research seems to be coming along well! Some formatting edits so that the video appears on the page would be good!
Overall, great work guys! Keep it up and move along with the project consistently!
- A good article for the overview of trunk neural crest cells
- Image about entire overview of neural crest migration
- find a image for the overview of DRG development
- if cannot find, use animal species to draw out the timeline
- work on chicken to identify origins of different components of DRG, neural crest
- timeline of discovery of DRG (use date of publication to put the timeline, around 1970s, original discovery is around 1930s)
- if cannot find about DRG, find about trunk neural crest
- which particular part of the neural crest contribute to the DRG
- do they differentiate during migration or do they differentiate only when reaching the location
- which particular mechanism influence the differentiation process into DRG
- extension of DRG to different end points (epithelium, joints, muscle fibres)
- good to include a timeline (schwann cells -> differentiation and myelination)
- understanding schwann cell differentiation and myelination
- neuronal cell death (apoptosis if they do not reach the cell type)
- Differentiation process
- When they start to function
Tissue / Organ structure
Molecular Mechanisms / Factors / Genes
"Blocking of CXCR4 by morpholino or shRNA in premigratory chick trunk neural crest cells leads to significantly fewer cells that reach the dorsal aorta and instead populate the dorsal root ganglia"
- summary of signalling pathway and their interactions with each other
- identifying if molecular factors are growth or transcription factors
Abnormalities / Abnormal development
Dorsal Root Ganglionopathy is responsible for sensory impairment in CANVAS
"Sensory ganglionitis, variably called ganglionopathy, is a disease of sensory neurons in dorsal root ganglia. Major forms of these diseases are associated with neoplasm, Sjögren syndrome, and paraproteinemia or polyclonal gammopathy with or without known autoantibodies. Most cases follow subacute courses, but there are forms that develop chronically and acutely as well. Clinical signs seen include sensory ataxia exhibited by gait unsteadiness, a positive Romberg sign, reduced deep tendon reflexes, poor coordination, and pseudo-athetoid movements in the hands. Axonal degeneration warrants the treatment as early as possible. Early cases of immunologic origin that are immune-mediated may respond to plasmapheresis and immunosuppression. Differential diagnoses include environmental and industrial intoxication and adverse effects of antineoplastic and antibiotic drugs. The term “sensory neuronopathy” or “ganglionitis” refers to disorders of small neurons, larger neurons, and/or neurons of both sizes in the sensory ganglia."
"In zebrafish, trunk NCCs start migrating along a medial pathway in-between the somites and the NT. These NCCs align to and are affected by slow muscle cells in the middle part of the somite"
"Hedgehog (Hh) signal transduction is directly required in zebrafish DRG precursors for proper development of DRG neurons. Zebrafish mutations in the Hh signaling pathway result in the absence of DRG neurons and the loss of expression of neurogenin1 (ngn1), a gene required for determination of DRG precursors. Cell transplantation experiments demonstrate that Hh acts directly on DRG neuron precursors. Blocking Hh pathway activation at later stages of embryogenesis with the steroidal alkaloid, cyclopamine, further reveals that the requirement for a Hh signal response in DRG precursors correlates with the onset of ngn1 expression. These results suggest that Hh signaling may normally promote DRG development by regulating expression of ngn1 in DRG precursors."
Hedgehog signaling is directly required for the development of zebrafish dorsal root ganglia neurons. Josette M. Ungos, Rolf O. Karlstrom, David W. Raible. Development 2003 130: 5351-5362; doi: 10.1242/dev.00722
"Dorsal root ganglia (DRGs) arise from trunk neural crest cells that emerge from the dorsal neuroepithelium and coalesce into segmental streams that migrate ventrally along the developing somites. Proper formation of DRGs involves not only normal trunk neural crest migration, but also the ability of DRG progenitors to pause at a particular target location where they can receive DRG-promoting signals. In mammalian embryos, a receptor tyrosine kinase proto-oncogene, ErbB3, is required for proper trunk neural crest migration. Here, we show that in zebrafish mutants lacking ErbB3 function, neural crest cells do not pause at the location where DRGs normally form and DRG neurons are not generated. We also show that these mutants lack trunk neural crest-derived sympathetic neurons, but that cranial neural crest-derived enteric neurons appear normal. We isolated three genes encoding neuregulins, ErbB3 ligands, and show that two neuregulins function together in zebrafish trunk neural crest cell migration and in DRG formation. Together, our results suggest that ErbB3 signaling is required for normal migration of trunk, but not cranial, neural crest cells."
"cdon is expressed in developing premigratory NCCs but is downregulated once the cells become migratory. Knockdown of cdon results in aberrant migration of trunk NCCs: crestin positive cells can emigrate out of the neural tube but stall shortly after the initiation of migration. Live cell imaging analysis demonstrates reduced directedness of migration, increased velocity and mispositioned cell protrusions. In addition, transplantation analysis suggests that cdon is required cell-autonomously for directed NCC migration in the trunk."
Powell DR, Williams JS, Hernandez-Lagunas L, Salcedo E, O'Brien JH & Artinger KB. (2015). Cdon promotes neural crest migration by regulating N-cadherin localization. Dev. Biol. , 407, 289-99. PMID: 26256768 DOI.
Current research (Labs)
- Brief, clear and concise
- HAVE A LIST OF ACRONYMS TO CONDENSE THE INFORMATION