Difference between revisions of "Talk:2009 Group Project 4"

From Embryology
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Current research  
Current research : I have put some information in the current research part. not sure where they should be put. Begum, as discussed you can edit it or place it in its appropriate place.
[http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=19538749| maternal diabetes alters transcriptional programs in the developing embryo], [http://www.ncbi.nlm.nih.gov/pubmed/19414407?ordinalpos=35&itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_DefaultReportPanel.Pubmed_RVDocSum|Abnormal mammary gland development in MMTV-CBLC transgenic mouse], [http://www.ncbi.nlm.nih.gov/pubmed/19394325?ordinalpos=40&itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_DefaultReportPanel.Pubmed_RVDocSum|Sonic hedgehog signalling inhibits palatogenesis and arrests tooth development in a mouse model of the nevoid basal cell carcinoma syndrome.], [http://www.ncbi.nlm.nih.gov/pubmed/19358209?ordinalpos=55&itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_DefaultReportPanel.Pubmed_RVDocSum| Fibroblast growth factor 18 gives growth and directional cues to airway cartilage.]  
[http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=19538749| maternal diabetes alters transcriptional programs in the developing embryo], [http://www.ncbi.nlm.nih.gov/pubmed/19414407?ordinalpos=35&itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_DefaultReportPanel.Pubmed_RVDocSum|Abnormal mammary gland development in MMTV-CBLC transgenic mouse], [http://www.ncbi.nlm.nih.gov/pubmed/19394325?ordinalpos=40&itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_DefaultReportPanel.Pubmed_RVDocSum|Sonic hedgehog signalling inhibits palatogenesis and arrests tooth development in a mouse model of the nevoid basal cell carcinoma syndrome.], [http://www.ncbi.nlm.nih.gov/pubmed/19358209?ordinalpos=55&itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_DefaultReportPanel.Pubmed_RVDocSum| Fibroblast growth factor 18 gives growth and directional cues to airway cartilage.]  

Revision as of 18:43, 22 September 2009

--Mark Hill 01:46, 8 September 2009 (EST) Well the content is there now, but what a mess, and I am not just talking about the formatting problem which can be easily fixed, you have no structure to your project, its not a matter of throwing everything at a wall and seeing what sticks. Work together for an integrated coverage. Timeline of development, is not the way to start your page with a huge table of data.

--Mark Hill 08:43, 21 August 2009 (EST) OK guys, time to see some actual content uploaded on both your discussion and project pages.

   * Timeline of Development - how long (Emily)
   * Staging - are there species specific staging, what occurs when (Elide)
   * History of Model Use - when was it first used, what embryology research (Begum)
   * Genetics - chromosome number, sequencing (Angama)
   * Current Embryology Research - research papers and findings (All)

Here is a link for timeline [http://books.google.com.au/books?id=XLIarRWHikAC&pg=PT199&lpg=PT199&dq=mouse+embryo+development+timeline&source=bl&ots=fobLBRiacx&sig=cK4cuZah6Ksczs3o8v4NXQqoAyk&hl=en&ei=rGB6SvTHMMmIkAXB_piAAw&sa=X&oi=book_result&ct=result&resnum=3#v=onepage&q=&f=false ]

link for the mouse brain development timeline http://en.wikipedia.org/wiki/Mouse_brain_development_timeline

hey guys there is another interesting link about mouse development http://mouseatlas.caltech.edu/index_content.html

Hey Emily. The link below has a timeline that you can check out in your spare time. Begum. [1]

Hi girls, here is a link to a text book about mouse embryology it looks pretty good. hope it can help [2] let me know if the link doesnt work. Ive been working on the main page, so have a look and tell me what you think, Also what are we doing about references? If we have used information but put it in our own words do we need to put in text citations,or do we just reference the journal at the end? I just want to be very careful. Thanks!

Hey Elide, i think your work is looking really good. its very easy to read and understand. keep going!!!! emily

hey everyone, I have uploaded some of my timeline work. I'm not sure if I've gone into to much detail or not and also on how is best to present the timeline. It is fairly basic and definately needs some work - especially on presentation, grammar, etc. let me know what you think. Emily

 good site for stages or timeline- atlas of pictures of stages- The Edinburgh Mouse Atlas Project [3]

Hello girls, it's Begum. I put some info under the history section. Wanted to let you all know that I've got a fair bit of info, and I will do my part as best as I can. Btw Emily, I think maybe dot from would be best for you, but if you can use those lines that I was talking to you about on Wednesday, that would be great...I know it's hard.

hey everyone, i have put some info under the genetics heading and some under research. I am still struggling to find the appropriate info related to the topic of genetics because there is alot of info abt the different types of stains used in labarotories but not the genetics. I emailed Dr.Hill and have asked him what to include in my section specifically,hopefully he will help. So far i have just started it needs alot of more work to be done,girls just read thru my section n leme know wt u think of it. have a nice weekend everyone. Angama.

Hi girls! Begum, you history info is really good. If very interesting! I really like how you are doing it in order of dates of discovery and what they did, what they found etc! Cant wait to know more. Angama, your doing well! it sounds like your finding the info hard to get. I'll keep an eye out for you! If your stuck on what you sound be doing then i might have a few ideas. I remember Mark Hill saying that you should compare the genome to the humans genome. so maybe if the genome is the same size as the humans, could you descibe similarities or differences? I know that there is a link to the mouse genome on the mouse web page he gave us ( next to the discussion link). Are you just ment to list the mouse genome sequence? could you go into what genes code what, eg which one codes for the sex linked gene, is it the X and Y gene etc? hopefully Mark gives you some ideas. well as you might have noticed i've been adding to my stages. the only thing is im worried about there being too much info up there. basically ive tried to get all the info available included in my stages to make sure i cover everything, and then later i'll go over it all and edit and polish it up a bit. Ive done some drawings to the best of my ability, but i can scrap them if you all think they arnt professional enough. just thought i'd try to present the information differently. let me know if you think i'm including too much information. i think i'm having the same problem as you begum, there is lots of info! Elide

Thanks Elide. I'm trying. (Again, loved the artwork!)Btw, Emily, I had a think about your section and I think it might be too much 'clicking' back-and-forth if we link the displayed pic to the 'info' page. Don't stress, you've got the info (heaps which is excellent) but make sure you get some pictures soon so we can start drawing (I'm helping with the drawings as well ok). Mark said 'Nature' and 'Science' have useable images so lets make that our start. BTW, I will be using the question mark symbol(???) so I don't forget to reference. Begum

Hey everyone, I have found an online text book. it has a chapter on genetics and history and a lot of other stuff. [4] Emily

Hey girls. If you've seen my section, the info is not on the main page, but linked to another page. I thought that it might make everything look more neat. I thought we could all do it like that. It's just an idea. Something different. Maybe we could have something on the main page (picture of a mouse). Your thoughts everybody? Begum

hey begum, ur work looks really good. i like the idea of linking the work to another page. - we don't have to worry about to much info being on the front page and it gives people to option of viewing the work if they want to. ive been working on drawings, i'll show them to you next week but am not sure how to upload them at the moment. Emily

Thanks! That sounds great that you like the idea. About the photos that you are drawing, if there by hand, you can scan them somehow. But overall, 1. click 'Upload File' on the left hand side of this page 2. New page comes up: click 'Browse' and choose your file that you want to upload. 3. Name it (under the Browse button) NOTE: write down what you named the file as because like Elide says "...it's going to be lost in space!..." 4. Write down info/comments (like who is the author (YOU), and if the drawing is based on a picture) 5. UPLOAD! 6. Go to your section and just normally type this down to the area you want the picture to be seen:


Your thoughts Angama and Elide? (about the linking of our sections to separate pages?) Begum.

Oh and another thing: What do you girls think about my page, I've got a heading for each DATE and underneath each there are further subheadings (e.g. 'What did he do?'. Should I change them to just text, I mean, does it look messy with sub-sub-headings? Begum.

wow girls! great work.. okay so i asked mark about a new page and he said to avoid it because our info is meant to be on our one page. he said if there is extra information on what we wanted to say but is too much for the main page then have a link to our discussion page. (which is what im going to do) Begum your new page is fantastic!! you have done lots of work! but why dont you just put it on our main page? also girls i think we are getting too carried away with info. just keep it simple! i'm sorry i havent been around this week to work on it but i plan to get going asap. just fixing up some things, summarising, writing introductions etc. did you all read his note about slabbing info onto our page?? how about some introductions, and sentences to ease ourselves into the content. planning on trying to work on that now anyway..

also lets get the information flowing. why dont we go intro, history of model use, stages, timeline, genetics, then current use.. what do you all think?

hey, i thing that that sounds like a good, logical way to do the page. i've finished the drawings, just need to upload them. About the timeline information, is there anything specific that i should include. the stuff that is on the page is a little vague and so i need some advice as to what are key points that i should include. i know that the drawing are very simplistic, let me know wjat you think about them. ive put one up below. i just need a way to link it to text. emily

Thanks Elide. You know, I think that's a great idea. Have it all on one page, seems less 'diverging...', seems more COMPLETE. I love the ORDER as well. I'll fix all that up!

Hey girls, please have a look at what i've done on the timeline. the images are all hand drawn based upon the text: 'the house mouse'. if anyone could give me any ideas on how best to present the pictures - which would be better - next to or below the text? keep in mind that it is incomplete and there is an illustration for each day of development (i.e. 19 in total). if you think that is too many let me know, some may be similar to Elide's ones. Also, do you think i should put some colour into the drawings? Emily

I love your work Emily. All you need to do is put the info on the growth of the mouse that is on the main page, onto the page with the graph of the growth of the mouse embryo...we talked about that before any way-AND I think your parts finished! Begum. Angama, I added something to the end of your section that I thought was interesting. Have a look. And are you mentioning manipulation and 'shut-down' of the genes in your section?? Begum. To everyone, apparently Mus Musculus is the scientific name of the common house mouse, not the mouse. I was thinking of editing that.

HEY EVERYONE! well i've added in some pictures just to make it look more visual, change them if you have better ones.. and i'm going to be working on the current research section tommorow. oh i also added some graphs like we thought of for emilys section.. so i think my section is finished finally! what do you think? can i just say, I think the whole thing looks great! you girls have been a pleasure to work with! :) Thanks so much!!

Current research : I have put some information in the current research part. not sure where they should be put. Begum, as discussed you can edit it or place it in its appropriate place.

maternal diabetes alters transcriptional programs in the developing embryo, mammary gland development in MMTV-CBLC transgenic mouse, hedgehog signalling inhibits palatogenesis and arrests tooth development in a mouse model of the nevoid basal cell carcinoma syndrome., Fibroblast growth factor 18 gives growth and directional cues to airway cartilage.

Staging of mouse embryo development

Theiler stage 6-11

Table 2: Mouse embryonic staging from blastocyst implantation to pre-somite formation (Theiler stages 6 to 11)
Theiler Stage Embryonic age in Days Post Coitum (dpc) Stage Characteristic Cell characteristics
6 4.5 (range 4-5.5)

Human carnegie stage: 4

Attachment of blastocyst


Embryonic Endoderm present covering the blastocoelic cells of the inner cell mass.
7 5 (range 4.5-6)

Human carnegie stage: 5


-Egg cylinder formation

-Ectoplacental cone

Inner cell mass increases in size

-Epiblast formation (enlarged mass)

-Proximal cells are cuboidal in shape

-Mural trophectoderm is lined by primary endoderm

8 6 (range 5-6.5)

Human carnegie stage: 5

Differentiation of egg cylinder into embryonic and extra-embryonic regions

-Pro-amniotic cavity formation

Trophoblast giant cells invade maternal tissue

-Maternal blood invades the ectoplacental cone

-Reichert's membrane appears

-Implantation site is 2x3mm

9 a) Pre-streak Advanced Endometrial and egg cylinder stage

-First evidence of embryonic axis

Morphological difference can be seen between embryonic and extra-embryonic ectoderm

-Maternal blood further invades ectoplacental cone

-Uterine crypts lose their original lumen

9 b) Early streak Gastrulation begins (later in stage) First mesodermal cells produced
10 a) 7 (range 6.5-7.5)

Mid streak to late streak Human carnegie stage: 8

Amnion formation The amniotic fold starts to form from posterior tissue of primitive streak bulging.

-Allantoic bud evident -Gastrulation continues -Primitive node visible -Amnion begins to close

11 7.5 (range 7.25-8)

Human carnegie stage: 9

Formation of neural plate and presomites Amniotic cavity is sealed to form 3 cavities (amniotic cavity, exocoelom and ectoplacental cleft)

-Allantoic bud elongates -Notochodal plate can be seen in the midline and subjacent to neural groove -Head form from the enlargement of the rostral end of neural plate (early head fold) -Formation of foregut pocket begins

Theiler stage 12-14

Theiler stage Embryonic age in Days Post Coitum (dpc) Stage characteristic Cell characteristics Number of somite pairs
12 a) 8 (range 7.5-8.75)

Human carnegie stage: 9

unturned embryo

-1st appearance of somite pairs

allantois extends into exocoelom

-maxillary components of 1st brachial arch prominent -visible preotic sulcus in 2-3 stomite embryo -formation of cardiogenic plate begins - foregut pocket visible

12 b) 8 (range 7.5-8.75) unturned embryo

- formation of somites 5-7 -abscent 2nd branchial arch

prominent headfolds

-neural closure at site of 4th and 5th somites closing in caudal and rostral directions -optic placodes visible with indentation of optic pits -rapid development of heart rudiment -allantois comes in contact with chorion

13 8.5 (range 8-9.25)

Human carnegie stage: 10

turning of embryo at around 6-8 pairs

-3rd branchial arch absent

1st branchial arch with maxillary and mandibular components

-2nd branchial arch visible - regionalization of heart visible -neural tube closure at point opposite outflow tract to proximal part of tail -notocord and prepancreatic endoderm contact remaining

14 9 (range 8.5-9.75)

Human carnegie stage: 11

anterior neuropore formation and closure (at 15-18 somite pairs)

-forelimb bud absent

optic pit becomes more indented

-mandibular process of 1st branchial arch visible -3rd branchial arch visible -prominent ridge on lateral body wall at 8th-12th somite


Theiler stages 15-20

Table 3: Mouse embryonic stages from Theiler stage 15 to 20 ( somite stages)

Theiler Stage Embryonic Age (dpc) Stage Characteristic Cell Characteristic Somite Number(pairs
15 9.5 (range 9-10.25)

Human carnegie stage: 12

Formation of Forelimb bud

-Posterior neuropore

8-12th somite pair condensation of forelimb bud is visible

-Hind limb bud appears

-Forebrain vesicle division into telencephalic and diencephalic vesicles

-Lung development commences

-1st sign of Pancreas morphogenesis of dorsal pancreatic bud (22-25 somites).

16 10 (range 9.5-10.75)

Human carnegie stage: 13-15

Caudal neuropore closes

-Hind limb bud (23rd-28th somite) and tail bud

Concave 3rd and 4th branchial arches.

-Rathke's pouch formation

-Nasal processes formation.

-Ventral pancreatic bud appears

17 10.5 (range 10-11.25)

Human carnegie stage: 13-15

Deep Lens Indentation Lens pit is deepened and has a narrowed outer opening.

-Physiological umbilical hernia present.

-1st branchial arch divides into maxillary and mandibular components.

-Advanced development of brain tube

-Tail elongates and thins

18 11 (range 10.5-11.25)

Human carnegie stage: 13-15

Closure of Lens Vesicle Cervical somites no longer visible

-Brain rapidly grows

-Formation of nasal pit

19 11.5 (range 11-12.25)

Human carnegie stage: 16

Lens vesicle separated completely from surface

–Closed and detached from ectoderm

Well Defined eyes and their peripheral margins

-Forelimbs divided into two regions

-Proximal part of the future limb-girdle and 'arm'

-Peripheral part forming a circular or anterior footplate.

-Otic pit medial and lateral margins move together

-Auditory hillocks visible

20 12 (range 11.5-13)

Human carnegie stage: 17

First sign of fingers Anterior footplate no longer circular (develops angles)

-Posterior footplate visible

-Pigmentation of retina visible

-Tongue and brain vesicles identifiable