Spermatozoa Meiosis Movie 1

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<html5media height="400" width="460">File:Spermatozoa_meiosis 01.mp4</html5media>

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Live imaging of wild type spermatocyte

Images acquired every 30 sec for 3.5 min.

GFP-Telomeric repeat-binding factor 1 (GFP-TRF1) shows the ends of the individual chromosomes.

GFP-synaptonemal complex protein SCP3 (GFP-SCP3) shows the synaptonemal complex, a meiosis-specific protein structure essential for synapsis of homologous chromosomes.


Wild type spermatocytes (20 dpp) co-expressing GFP-Telomeric repeat-binding factor 1 (GFP-TRF1) and GFP-SCP3 cultured in medium supplemented with Hoechst 33324. Images were collected using a DeltaVision microscopy system. Exposures of 0.15 sec (for GFP) and 0.025 sec (for Hoechst 33324) were acquired every 30 sec for 3.5 min.


Links: MP4 | Meiosis | Spermatozoa Development | Mouse Development | Movies

Reference

<pubmed>25502938</pubmed>

PLoS Genet. DOI: 10.1371/journal.pgen.1004821

Copyright

© 2014 Shibuya et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Original AVI converted to MP4



Cite this page: Hill, M.A. (2024, March 29) Embryology Spermatozoa Meiosis Movie 1. Retrieved from https://embryology.med.unsw.edu.au/embryology/index.php/Spermatozoa_Meiosis_Movie_1

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© Dr Mark Hill 2024, UNSW Embryology ISBN: 978 0 7334 2609 4 - UNSW CRICOS Provider Code No. 00098G