Spermatozoa Meiosis Movie 1: Difference between revisions
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{{Movie header}} | {{Movie header}} | ||
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| width= | | width=470px|<html5media height="400" width="460">File:Spermatozoa_meiosis 01.mp4</html5media> | ||
[[Media:Spermatozoa_meiosis 01.mp4|Play]] | [[Media:Spermatozoa_meiosis 01.mp4|Mobile Play]] | ||
| Live imaging of wild type spermatocyte. Wild type spermatocytes (20 dpp) co-expressing GFP-TRF1 and GFP-SCP3 cultured in medium supplemented with Hoechst 33324. Images were collected using a DeltaVision microscopy system. Exposures of 0.15 sec (for GFP) and 0.025 sec (for Hoechst 33324) were acquired every 30 sec for 3.5 min. | | valign=top| | ||
===Live imaging of wild type spermatocyte=== | |||
Images acquired every 30 sec for 3.5 min. | |||
'''GFP-Telomeric repeat-binding factor 1''' (GFP-TRF1) shows the ends of the individual chromosomes. | |||
'''GFP-synaptonemal complex protein SCP3''' (GFP-SCP3) shows the synaptonemal complex, a meiosis-specific protein structure essential for synapsis of homologous chromosomes. Male primordial germ cells express SCP3 for a short time before undergoing G1 arrest. (More? SCP3 PMID 10794082) | |||
Wild type spermatocytes (20 dpp) co-expressing GFP-Telomeric repeat-binding factor 1 (GFP-TRF1) and GFP-SCP3 cultured in medium supplemented with Hoechst 33324. Images were collected using a DeltaVision microscopy system. Exposures of 0.15 sec (for GFP) and 0.025 sec (for Hoechst 33324) were acquired every 30 sec for 3.5 min. | |||
:'''Links:''' [[Media:Spermatozoa_meiosis 01.mp4|MP4]] | [[Cell Division - Meiosis|Meiosis]] | [[Spermatozoa Development]] | [[Mouse Development]] | [[Movies]] | |||
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| colspan=2|'''Prophase I''' is divided into 5 stages (leptotene, zygotene, pachytene, diplotene, diakinesis) The bouquet stage occurs at the transition from leptotene to zygotene stage, where the chromosome telomeres attachment to the inner nuclear envelope and form a cluster and occurs before the onset of homologous pairing and synapsis. The name comes from the chromosomes resembling a "bouquet of flowers". | |||
:'''Prophase I Movies:''' [[Media:Spermatozoa meiosis leptotene-zygotene 01.mp4|leptotene-zygotene stage]] | [[Media:Spermatozoa meiosis bouquet stage 01.mp4|bouquet stage]] | [[Media:Spermatozoa meiosis pachytene stage 01.mp4|pachytene stage]] | [[Media:Spermatozoa meiosis pachytene stage 02.mp4|pachytene stage]] | [[Media:Spermatozoa meiosis diplotene stage 01.mp4|diplotene]] | |||
{{Cell Division terms}} | |||
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[[File:Spermatocyte prophase 1 stages 01.jpg|800px]] | |||
{| | |||
| width=310px|<html5media height="320" width="300">File:Spermatozoa meiosis leptotene-zygotene 01.mp4</html5media> | |||
| width=310px|<html5media height="320" width="300">File:Spermatozoa meiosis bouquet stage 01.mp4</html5media> | |||
|- | |||
| Leptotene-zygotene | |||
| Bouquet stage | |||
|- | |||
| width=310px|<html5media height="320" width="300">File:Spermatozoa meiosis pachytene stage 01.mp4</html5media> | |||
| width=310px|<html5media height="320" width="300">File:Spermatozoa meiosis diplotene stage 01.mp4</html5media> | |||
|- | |||
| Pachytene stage | |||
| Diplotene stage | |||
|} | |} | ||
===Reference=== | ===Reference=== | ||
<pubmed>25502938</pubmed> | <pubmed>25502938</pubmed> | ||
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====Copyright==== | ====Copyright==== | ||
© 2014 Shibuya et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Original AVI converted to MP4 | © 2014 Shibuya et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Original AVI converted to MP4 | ||
{{Footer}} | {{Footer}} | ||
[[Category:Mouse]][[Category:Spermatozoa]][[Category:Meiosis]][[Category:Movies]] | [[Category:Mouse]][[Category:Spermatozoa]][[Category:Meiosis]][[Category:Movies]] |
Latest revision as of 16:56, 18 March 2017
Embryology - 24 Apr 2024 Expand to Translate |
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<html5media height="400" width="460">File:Spermatozoa_meiosis 01.mp4</html5media> |
Live imaging of wild type spermatocyteImages acquired every 30 sec for 3.5 min. GFP-Telomeric repeat-binding factor 1 (GFP-TRF1) shows the ends of the individual chromosomes. GFP-synaptonemal complex protein SCP3 (GFP-SCP3) shows the synaptonemal complex, a meiosis-specific protein structure essential for synapsis of homologous chromosomes. Male primordial germ cells express SCP3 for a short time before undergoing G1 arrest. (More? SCP3 PMID 10794082)
| |||||
Prophase I is divided into 5 stages (leptotene, zygotene, pachytene, diplotene, diakinesis) The bouquet stage occurs at the transition from leptotene to zygotene stage, where the chromosome telomeres attachment to the inner nuclear envelope and form a cluster and occurs before the onset of homologous pairing and synapsis. The name comes from the chromosomes resembling a "bouquet of flowers".
|
<html5media height="320" width="300">File:Spermatozoa meiosis leptotene-zygotene 01.mp4</html5media> | <html5media height="320" width="300">File:Spermatozoa meiosis bouquet stage 01.mp4</html5media> |
Leptotene-zygotene | Bouquet stage |
<html5media height="320" width="300">File:Spermatozoa meiosis pachytene stage 01.mp4</html5media> | <html5media height="320" width="300">File:Spermatozoa meiosis diplotene stage 01.mp4</html5media> |
Pachytene stage | Diplotene stage |
Reference
<pubmed>25502938</pubmed>
PLoS Genet. DOI: 10.1371/journal.pgen.1004821
Copyright
© 2014 Shibuya et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Original AVI converted to MP4
Cite this page: Hill, M.A. (2024, April 24) Embryology Spermatozoa Meiosis Movie 1. Retrieved from https://embryology.med.unsw.edu.au/embryology/index.php/Spermatozoa_Meiosis_Movie_1
- © Dr Mark Hill 2024, UNSW Embryology ISBN: 978 0 7334 2609 4 - UNSW CRICOS Provider Code No. 00098G