Spermatozoa Meiosis Movie 1: Difference between revisions

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Embryology - 19 Apr 2024 Expand to Translate
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<html5media height="400" width="400">File:Spermatozoa_meiosis 01.mp4</html5media>

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Live imaging of wild type spermatocyte. Wild type spermatocytes (20 dpp) co-expressing GFP-TRF1 and GFP-SCP3 cultured in medium supplemented with Hoechst 33324. Images were collected using a DeltaVision microscopy system. Exposures of 0.15 sec (for GFP) and 0.025 sec (for Hoechst 33324) were acquired every 30 sec for 3.5 min.

Reference

PLoS Genet. DOI: 10.1371/journal.pgen.1004821

Copyright

© 2014 Shibuya et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Original AVI converted to MP4 (200 × 200)

Cite this page: Hill, M.A. (2024, April 19) Embryology Spermatozoa Meiosis Movie 1. Retrieved from https://embryology.med.unsw.edu.au/embryology/index.php/Spermatozoa_Meiosis_Movie_1

What Links Here?

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-<html5media height="400" width="400">File:Spermatozoa_meiosis 01.mp4</html5media>
+{{Movie header}}
+{|
+| <html5media height="400" width="400">File:Spermatozoa_meiosis 01.mp4</html5media>
 [[Media:Spermatozoa_meiosis 01.mp4|Play]]
+| Live imaging of wild type spermatocyte. Wild type spermatocytes (20 dpp) co-expressing GFP-TRF1 and GFP-SCP3 cultured in medium supplemented with Hoechst 33324. Images were collected using a DeltaVision microscopy system. Exposures of 0.15 sec (for GFP) and 0.025 sec (for Hoechst 33324) were acquired every 30 sec for 3.5 min.
- × 200
+|}
-===The dissection of meiotic chromosome movement in mice using an in vivo electroporation technique===
-PLoS Genet. 2014 Dec 11;10(12):e1004821. doi: 10.1371/journal.pgen.1004821. eCollection 2014.
-Shibuya H1, Morimoto A1, Watanabe Y1.
-Abstract
-During meiosis, the rapid movement of telomeres along the nuclear envelope (NE) facilitates pairing/synapsis of homologous chromosomes. In mammals, the mechanical properties of chromosome movement and the cytoskeletal structures responsible for it remain poorly understood. Here, applying an in vivo electroporation (EP) technique in live mouse testis, we achieved the quick visualization of telomere, chromosome axis and microtubule organizing center (MTOC) movements. For the first time, we defined prophase sub-stages of live spermatocytes morphologically according to GFP-TRF1 and GFP-SCP3 signals. We show that rapid telomere movement and subsequent nuclear rotation persist from leptotene/zygotene to pachytene, and then decline in diplotene stage concomitant with the liberation of SUN1 from telomeres. Further, during bouquet stage, telomeres are constrained near the MTOC, resulting in the transient suppression of telomere mobility and nuclear rotation. MTs are responsible for these movements by forming cable-like structures on the NE, and, probably, by facilitating the rail-tacking movements of telomeres on the MT cables. In contrast, actin regulates the oscillatory changes in nuclear shape. Our data provide the mechanical scheme for meiotic chromosome movement throughout prophase I in mammals.
-PMID 25502938 PMCID: PMC4263375 https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4263375/
-DOI: 10.1371/journal.pgen.1004821 http://journals.plos.org/plosgenetics/article?id=10.1371/journal.pgen.1004821
 ===Reference===
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 ====Copyright====
-© 2014 Shibuya et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
+© 2014 Shibuya et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Original AVI converted to MP4 (200 × 200)
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 [[Category:Mouse]][[Category:Spermatozoa]][[Category:Meiosis]][[Category:Movies]]