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- 19:08, 7 August 2012 Z3332337 talk contribs uploaded File:The effect of embryo manipulation on 5meC staining in zygotes z3332337.jpeg (Asymmetric anti-5meC staining of the male and female pronucleus after acid-pretreatment has been reported [5], [6], [20], [21] yet was not confirmed by this study. The zygotes used in past studies were commonly generated by in vitro fertilization or subje)