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- 13:13, 13 September 2016 Z8600021 talk contribs uploaded File:Limb induction-initiation signal 02.jpg (Figure 7. The Molecular Mechanisms of Limb Bud Formation (A) During limb induction, an axial signal is required for Raldh2 expression in the LPM, which produces RA locally. RA, b-catenin/TCF/LEF, and Hox genes act cooperatively to induce Tbx5 in the LP...)