Paper - A Human Ovum Nine to Ten Days Old

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Davies F. and Harding HE. A Human ovum nine to ten days old. (1944) BJOG: An International Journal of Obstetrics & Gynaecology 51(3): 225-230.

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A Human Ovum Nine to Ten Days Old


Francis Davies, M.D., D.Sc., F.R.S.E. And H. E. Harding, M.D.

Departments of Anatomy and Pathology, University of Sheffield

Clinical History

THE specimen was obtained from the uterus removed from a married woman, aged 26 years, admitted to hospital for pelvic pain. She had had 2 children, both at term, one in December 1938 and the other in March 1940. She had not had any abortions or miscarriages. At operation on September 9th, 1943, endometriosis of the left ovary was diagnosed and a corpus lutea was seen in the right ovary, but no details of the latter are available. The uterus was placed in 10 per cent formolsaline immediately after removal later a pin-head red spot was found in the thickened endometrium on the anterior wall of the uterus near the entrance of the right uterine tube; the uterus appeared otherwise normal. A block of tissue surrounding the red spot was removed, appropriately treated, and embedded in parafiin wax. Some sections were removed and only one of these mounted before the true value of the specimen was appreciated. The remainder of the paraffin block was then melted, the tissue orientated and re-embedded, and serial sections 9 micron thick were cut, stained with haemalum and eosin (Stain - Haematoxylin Eosin) and photographed. Study of these revealed that between one-third and one-half of the ovum had been removed in the preliminary treatment but that the remaining portion was suflicient in amount and in excellence of preservation to enable a thorough study of all the various embryonic elements to be made. The menstrual and coital history, summarized in the table, was obtained at the time from both husband and wife. The maximum coital age of the specimen is 9-Io days, an age which is also indicated by the histological structure of the ovum itself.


Menstrual, coital and operative data. Menstrual cycle 28 days.

Histological Examination

The endometrium (Fig. 2) has the structure characteristic of the secretory (premenstrual) phase of the cycle. It is 5 mm. thick at the implantation site and slightly thinner (4.5 mm.) elsewhere. Compact, spongy and basal strata are clearly differentiated and the “ physiological oedema ” between the stromal cells is minimal. The endometrial glands are lined by columnar epithelium throughout and contain secretion in the form of an eosinophilic, amorphous coagulum, except in the basal layer where secretion is absent. There is no maternal blood in the glands. Spiral arteries are prominent in the stromal partitions between the glands in the spongy layer, where the glands are tortuous and have the characteristic “ saw—tooth ” edges. The ovum is embedded solely in the stratum compactum. Definite decidual reaction is not present, but some of the stromal cells adjacent to the trophoblast have a broader halo of perinuclear cytoplasm than ordinary stromal cells (pre-decidual cells).

The trophoblast is clearly differentiated into cytotrophoblast and plasmoditrophoblast (Fig. r); all stages in the formation of the latter from the former are seen and mitotic figures are numerous in the cytotrophoblast. Solid clumps of cytotrophoblast project from the surface of the chorionic vesicle, but they are not penetrated by the primitive mesoblast,so thattrue villi are not yet formed. Lacunae of various sizes and stages of formation are present in the plasmoditrophoblast; only those at the abembryonic (superficial) pole of the ovum contain maternal blood, elsewhere they contain only leucocytes. The “ operculum,” which indicates the site of entry of the ovum into the endometrium, consists of a mixture of fibrin and leucocytes permeated by plasmoditrophoblast; no epithelialization has yet taken place to cover the surface of the operculum There is no zone of necrosis of the endometrium adjacent to the trophoblast such as was described in the Teacher-Bryce‘ and the Mollendorff Sch? ova, but the stromal cells adjacent to the trophoblast appear to be undergoing cytolysis as they are encroached upon by the trophoblast, and their nuclei show varying degrees of pale staining.

The chorionic cavity (Fig. 1) is permeated by the primitive mesoblast, which has the form of a loose meshwork consisting partly of large stellate cells and partly of smaller elongated cells; the mesoblast is continuous with the cytotrophoblast superficially and with the exocoelomic (Heuser’s) membrane deeply. The latter membrane is complete, unlike that in the 11.5 day Hertig-Rock ovum,“ and, together with the endodermal layer of the germ disc, it encloses the exocoelomic vesicle (primitive yolk-sac). Themembrane consists of flattened and elongated cells; it is continuous with the edge of the endodermal layer of the disc, the transition to the cubical endodermal cells being very sudden. This feature favours the view of Heuser‘ that the membrane is mesoblastic in origin, whereas the endoderm is derived from the inner cell mass.

The embryo proper (Fig. 3) consists of a bilaminar disc, comprising a single layer of tall columnar ectoderrnal cells which form the floor of the amniotic cavity, and a single layer of cubical endodermal cells which form the roof of the exocoelomic vesicle. Mesoderm does not penetrate between these two germ layers. Part of the disc is missing, but assuming it was circular, and such an assumption is warranted by comparison with other early human and with monkey

Fig 1. Ovum and endometrium. Operculum, consisting of fibrin and leucocytes permeated by plasmoditrophoblast, is seen in upper part of photograph. Fibrinous wisp projects into mouth of gland on left. No epithelium on surface of operculum. Sprouts of trophoblast seen penetrating stratum compactum below ovum. Maternal red-blood corpuscles seen in lucunae of plasmoditrophoblast to left of chorionic vesicle. Primi- tive mesoblast shown permeating chorionic vesicle between cytotrophoblast. and thin exocoelomic membrane. Of the two cavities inside the chorionic.vesicle, the upper, larger one is the exocoelomic vesicle (primitive yolk sac), with the endodcnnal plate of cubical cells forming its lower wall. The lower, smaller cavity i the amniotic cavity; in the picture its upper wall is formed by the columnar cells of the ectodermal disc. The endometrium adjacent to the trophoblast shows no zone of necrosis. x 90.

Fig 2. Ovum embedded in stratum compactum of endometrium: the endometrium shows the structure characteristic of the secretory phase. Spiral arteries, cut across several times in the section, are seen between two glands just to the right of the'centre.; x n.

Fig 3. Part of ovum. The tall columnar cells of the ectodermal disc are seen to continue at the edge of the disc into tlIe‘llattened cells of the amnion. The lower part (really roof) of the latter is seen to merge into the cytotrophoblast. and transition cells between the two are shown just to the right of the centre of the picture. In the upper part the cubical cells of the endodermal layer _of the disc are seen to continue into the flattened cells of the exocoelomic membrane. Eosinophilic deposit is seen in the exocoelomic vesicle (above), in the amniotic cavity. (flow) and in the meshes of the primitive mesoblast (above and to the right). x 430.


ova, the diameter of the disc would be 0.117 mm.; that of the disc in the 11.5 day Hertig-Rock ovum is o.I38 mm. At the edge of the disc the ectodermal cells suddenly change to the flattened cells comprising the side walls and roof of the amniotic cavity, a feature which supports the views of Ramsey‘ and Heuser and Streeter“ that in primates the ectodermal layer of the disc is formed from the inner cell mass, whereas the amnion is derived from the mesoblast, which in its turn has been delaminated from the cytotrophoblast. In the present specimen the roof of the amnion is deficient in part ; here the cavity is roofed over by cytotrophoblast and transition cells between cytotrophoblast and amniotic cells are

‘present. Definite yolk sac has not yet been



The smaller dimensions of the chorionic vesicle and of the embryonic disc, and the lesser degree of enlargement of the endometrial sinusoids in the present specimen indicate that it is younger than the II. 5 day Hertig-Rock ovum. More recently, Rock and Hertig’ have published a preliminary report, including a photomicrograph of one section, of an implanted human ovum (Wi-8004) estimated to be 9.5 days old, but a detailed account of its structure and measurements is not given. Inspection of this photograph shows that there are many similarities with the present specimen. The 9. 5 day Rock-Hertig ovum and the present specimen comprise the earliest stages of fully‘ implanted human ova so far discovered. The incomplete Miller ovum“ was tentatively concluded by Streeter’ in 1926 to have an ovulation age of IO to II days. After

° The term "fully implanted," as used in this paper, implies that no part of the ovum is freely exposed to the uterine cavity.


Streeter’s‘° new profile reconstruction of the Miller ovum in 1939, Hertig and Rock“ noted that it closely resembled their II. 5 day specimen and quote Streeter as now being of the opinion that the Miller ovum is slightly older than their specimen.

Streeter“ expressed a the View that the differences in details of the form and in the rate of differentiation and growth between the embryos of man and monkey do not become appreciable until the end of the 2nd month, and that therefore for at least the Ist month or 6 weeks, the ages known for macaque embryos may be transferred to human ‘embryos of corresponding development. The present specimen is similar in its general features to the Io-day macaque embryo described by Heuser and Streeter, but it is more advanced, however, in respect of the development of the amniotic cavity and of the primitive mesoblast. Heuser“’” has stressed the precocious formation of the primitive mesoblast in the monkey»; the present-specimen indicates that mesoblast formation in the human is even more precocious than that in the monkey, and, taken in conjunction with the human ova described by Hertig and Rock, it indicates that there are considerable differences in general organization of the human embryo between the ages of 9.5 and 12.5 days and that of macaque embryos of corresponding ages. Heuser and Streeter‘ illustrate variations in the state of development of the various elements in macaque embryos of the same age; likewise the present specimen shows some differences of detail from the 9. 5-day Rock-Hertig ovum in that it is more deeply implanted, surface epithelialization has not yet occurred, the trophoblast on the abembryonic side is thicker, the exocoelomic cavity is larger and the endoderrn consists for the main part of only a single layer of cells.

The exocoelomic‘ (Heuser’s) membrane, unlike that in the 11.5-day Hertig-Rock

Cite this page: Hill, M.A. (2021, June 22) Embryology Paper - A Human Ovum Nine to Ten Days Old. Retrieved from

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