Oocyte Meiosis Movie 1: Difference between revisions

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<html5media height="400" width="800">File:Oocyte_Meiosis_01.mp4</html5media>

An oocyte undergoing spindle migration followed by first polar body extrusion and MII spindle positioning.

blue - Hoechst staining of chromosomes.
green - F-actin probe (UtrCH-GFP, utrophin calponin homology domain fused to GFP) label cortical changes during spindle migration.

The video shows that cytoplasmic streaming continues to the MII arrest stage to maintain the oocyte set of chromosomes/MII spindle in place close to the cortex.

Frames are 11 min apart, and video length is 840 min. Bar, 20 µm.

Meiosis Links: Oocyte Meiosis Movie | 0 min | 270 min | 280 min | 320 min | 840 min | Meiosis spindle movement model | oocyte | meiosis | mouse | Movies

Reference

<pubmed>23439682</pubmed>| PMC3587830 | J Cell Biol.

Copyright

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Video 7 Original video altered in size and movie format.

@@ Line 8: / Line 8: @@
 * <font color=blue>'''blue'''</font> - Hoechst staining of chromosomes.
-* <font color=green>'''green'''</font> - UtrCH-GFP was used to label cortical changes during spindle migration.
+* <font color=green>'''green'''</font> -  F-actin probe (UtrCH-GFP, utrophin calponin homology domain fused to GFP) label cortical changes during spindle migration.
 The video shows that cytoplasmic streaming continues to the MII arrest stage to maintain the oocyte set of chromosomes/MII spindle in place close to the cortex.