Oocyte Meiosis Movie 1: Difference between revisions

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<html5media height="400" width="800">File:Oocyte_Meiosis_01.mp4</html5media>

An oocyte undergoing spindle migration followed by first polar body extrusion and MII spindle positioning.

blue - Hoechst staining of chromosomes.
green - UtrCH-GFP was used to label cortical changes during spindle migration.

The video shows that cytoplasmic streaming continues to the MII arrest stage to maintain the oocyte set of chromosomes/MII spindle in place close to the cortex.

Frames are 11 min apart, and video length is 840 min. Bar, 20 µm.

Reference

<pubmed>23439682</pubmed>| PMC3587830 | J Cell Biol.

Copyright

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Video 7 Original video altered in size and movie format.

@@ Line 5: / Line 5: @@
 | [[File:Oocyte Meiosis 01 icon.jpg|100px|left]] An oocyte undergoing spindle migration followed by first polar body extrusion and MII spindle positioning.
-The UtrCH-GFP (green) was used to label cortical changes during spindle migration. Kymograph of the UtrCH-GFP was provided as in Fig. S2 L.
+* <font color=blue>'''blue'''</font> - Hoechst staining of chromosomes.
+* <font color=green>'''green'''</font> - UtrCH-GFP was used to label cortical changes during spindle migration.
 The video shows that cytoplasmic streaming continues to the MII arrest stage to maintain the oocyte set of chromosomes/MII spindle in place close to the cortex.