Oocyte Meiosis Movie 1: Difference between revisions

Revision as of 12:02, 30 July 2014

An oocyte undergoing spindle migration followed by first polar body extrusion and MII spindle positioning. The UtrCH-GFP (green) was used to label cortical changes during spindle migration. Kymograph of the UtrCH-GFP was provided as in Fig. S2 L. The video again shows that cytoplasmic streaming continues to the MII arrest stage to maintain the oocyte set of chromosomes/MII spindle in place close to the cortex. Frames are 11 min apart, and video length is 840 min. Bar, 20 µm.

Reference

<pubmed>23439682</pubmed>| PMC3587830 | J Cell Biol.

Copyright

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Video 7 Original video altered in size and movie format.

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+An oocyte undergoing spindle migration followed by first polar body extrusion and MII spindle positioning. The UtrCH-GFP (green) was used to label cortical changes during spindle migration. Kymograph of the UtrCH-GFP was provided as in Fig. S2 L. The video again shows that cytoplasmic streaming continues to the MII arrest stage to maintain the oocyte set of chromosomes/MII spindle in place close to the cortex. Frames are 11 min apart, and video length is 840 min. Bar, 20 µm.
-===Sequential actin-based pushing forces drive meiosis I chromosome migration and symmetry breaking in oocytes===
-J Cell Biol. 2013 Mar 4;200(5):567-76. doi: 10.1083/jcb.201211068. Epub 2013 Feb 25.
-Yi K, Rubinstein B, Unruh JR, Guo F, Slaughter BD, Li R.
-Abstract
-Polar body extrusion during oocyte maturation is critically dependent on asymmetric positioning of the meiotic spindle, which is established through migration of the meiosis I (MI) spindle/chromosomes from the oocyte interior to a subcortical location. In this study, we show that MI chromosome migration is biphasic and driven by consecutive actin-based pushing forces regulated by two actin nucleators, Fmn2, a formin family protein, and the Arp2/3 complex. Fmn2 was recruited to endoplasmic reticulum structures surrounding the MI spindle, where it nucleated actin filaments to initiate an initially slow and poorly directed motion of the spindle away from the cell center. A fast and highly directed second migration phase was driven by actin-mediated cytoplasmic streaming and occurred as the chromosomes reach a sufficient proximity to the cortex to activate the Arp2/3 complex. We propose that decisive symmetry breaking in mouse oocytes results from Fmn2-mediated perturbation of spindle position and the positive feedback loop between chromosome signal-induced Arp2/3 activation and Arp2/3-orchestrated cytoplasmic streaming that transports the chromosomes.
-PMID 23439682
-http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3587830
-http://jcb.rupress.org/content/200/5/567.full
 ===Reference===
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 <pubmed>23439682</pubmed>| [http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3587830 PMC3587830] | [http://jcb.rupress.org/content/200/5/567.full J Cell Biol.]
-====Copyright====
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-{{JCB}}
+[http://rup-movie.glencoesoftware.com/video/10.1083/jcb.201211068/video-7 Video 7] Original video altered in size and movie format.