Neural Tube Closure Movie: Difference between revisions

Latest revision as of 16:45, 24 April 2018

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<html5media height="440" width="380">File:Mouse neural tube 01.mp4</html5media>

Click Here to play on mobile device

Mouse Neural Tube Closure

Live imaging of the sealing midbrain–hindbrain neuropore (MHNP) by closures I and II.

Time-lapse video of a SCAT3tg/+ embryo undergoing MHNP closure, as shown in paper Fig. 2 F. ECFP images are shown. Live imaging was performed on an inverted confocal microscope (TCS SP5). Frames were taken at intervals of 4 min for 14 h.

Reference

Yamaguchi Y, Shinotsuka N, Nonomura K, Takemoto K, Kuida K, Yosida H & Miura M. (2011). Live imaging of apoptosis in a novel transgenic mouse highlights its role in neural tube closure. J. Cell Biol. , 195, 1047-60. PMID: 22162136 DOI.

Copyright

Rockefeller University Press - Copyright Policy This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jcb.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at https://creativecommons.org/licenses/by-nc-sa/4.0/ ). (More? Help:Copyright Tutorial)

Video 2. http://jcb.rupress.org/content/suppl/2011/12/08/jcb.201104057.DC1/JCB_201104057_V2.mov

Movie versions: MP4 | OGG | Movies

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Cite this page: Hill, M.A. (2024, April 19) Embryology Neural Tube Closure Movie. Retrieved from https://embryology.med.unsw.edu.au/embryology/index.php/Neural_Tube_Closure_Movie

What Links Here?

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+__NOTOC__{{Movie header}}
 {|
-| [[Media:Mouse neural tube 01.mp4]]
+| width=400px|<html5media height="440" width="380">File:Mouse neural tube 01.mp4</html5media>
-|
+[[Media:Mouse neural tube 01.mp4|'''Click Here''' to play on mobile device]]
+| valign=top|
 ===Mouse Neural Tube Closure===
 [[File:Mouse_neural_tube_01_movie_icon.jpg|thumb]]
-Live imaging of the sealing midbrain–hindbrain neuropore (MHNP) by closures I and II.
+Live imaging of the sealing midbrain–hindbrain neuropore ('''MHNP''') by closures I and II.
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 {{Mouse neural tube 01 links}}
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+<br><br>
 ===Reference===
-<pubmed>22162136 </pubmed>| [http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3241723 PMC3241723] | [http://jcb.rupress.org/content/195/6/1047.long J Cell Biol.]
+{{#pmid:22162136}}
 {{JCB}}
 Video 2. http://jcb.rupress.org/content/suppl/2011/12/08/jcb.201104057.DC1/JCB_201104057_V2.mov
-Movie Versions: [[:File:Mouse neural tube 01.mp4|MP4]] | [[:File:Mouse neural tube 01.mov|QT]] | [[:File:Mouse neural tube 01.flv|Flash]] | [[:File:Mouse neural tube 01.ogg|OGG]] | [[Movies]]
+Movie versions: [[:File:Mouse neural tube 01.mp4|MP4]] | [[:File:Mouse neural tube 01.ogg|OGG]] | [[Movies]]
-----
-===Live imaging of apoptosis in a novel transgenic mouse highlights its role in neural tube closure===
-J Cell Biol. 2011 Dec 12;195(6):1047-60. doi: 10.1083/jcb.201104057.
-Yamaguchi Y, Shinotsuka N, Nonomura K, Takemoto K, Kuida K, Yosida H, Miura M.
-Source
-Department of Genetics, Graduate School of Pharmaceutical Sciences, The University of Tokyo, Bunkyo-ku, Tokyo 113-0033, Japan. bunbun@mol.f.u-tokyo.ac.jp
-Abstract
-Many cells die during development, tissue homeostasis, and disease. Dysregulation of apoptosis leads to cranial neural tube closure (NTC) defects like exencephaly, although the mechanism is unclear. Observing cells undergoing apoptosis in a living context could help elucidate their origin, behavior, and influence on surrounding tissues, but few tools are available for this purpose, especially in mammals. In this paper, we used insulator sequences to generate a transgenic mouse that stably expressed a genetically encoded fluorescence resonance energy transfer (FRET)-based fluorescent reporter for caspase activation and performed simultaneous time-lapse imaging of apoptosis and morphogenesis in living embryos. Live FRET imaging with a fast-scanning confocal microscope revealed that cells containing activated caspases showed typical and nontypical apoptotic behavior in a region-specific manner during NTC. Inhibiting caspase activation perturbed and delayed the smooth progression of cranial NTC, which might increase the risk of exencephaly. Our results suggest that caspase-mediated cell removal facilitates NTC completion within a limited developmental window.
+{{Movie footer}}
-PMID 22162136
 [[Category:Mouse]] [[Category:Neural]] [[Category:MP4]]