Musculoskeletal System - Bone Development: Difference between revisions

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== Introduction ==
== Introduction ==
[[File:Endochondral_bone.jpg|thumb|Endochondral bone]]
[[File:Endochondral_bone.jpg|thumb|Endochondral bone]]
[[File:Bone-femur-c.jpg|thumb|link=File:Bone-femur.jpg]]
[[File:Bone-femur.jpg|thumb|alt=Bone femur diagram|Bone femur]]
The mesoderm forms nearly all the connective tissues of the musculoskeletal system, except within the head where neural crest also contributes connective tissues. Each tissue (cartilage, bone, and muscle) goes through many different mechanisms of differentiation.
The skeleton consists of {{bone}} developing from {{mesoderm}}, except within the head where {{neural crest}} also contributes connective tissues. Each tissue ({{cartilage}}, {{bone}}, and {{skeletal muscle}}) goes through many different mechanisms of differentiation.


The 2 key developmental processes are the initial "patterning" of bone location and then the overt "differentiation" of bone through the process of ossification.


Bone is formed through a lengthy process involving ossification of a cartilage formed from mesenchyme. Two main forms of ossification occur in different bones, intramembranous (eg skull) and endochondrial (eg limb long bones) ossification. Ossification continues postnatally, through puberty until mid 20s. Early ossification occurs at the ends of long bones.
The 2 key developmental processes are the initial "patterning" of bone location and then the overt "differentiation" of bone through the process of ossification. For details on specific bone differentiation in human development see [[Musculoskeletal System - Bone Development Timeline|Bone Development Timeline]].
 
 
Bone is formed through a lengthy process involving ossification of a cartilage formed from mesenchyme. Two main forms of ossification occur in different bones, {{intramembranous ossification}} (eg skull) and {{endochondral ossification}} (eg limb long bones) ossification. Ossification continues postnatally, through puberty until mid 20's. Early ossification occurs at the ends of long bones. A study of 18,713 individuals identified the male/female age at attainment of peak {{bone mineral density}} (BMD).{{#pmid:31760214|PMID31760214}}
 


The two major parts of the human skeleton are the axial (80 bones in skull, vertebra, ribs, sternum) and appendicular (126 bones in limbs, shoulders, pelvis) skeletons.
The two major parts of the human skeleton are the axial (80 bones in skull, vertebra, ribs, sternum) and appendicular (126 bones in limbs, shoulders, pelvis) skeletons.


Musculoskeletal and limb abnormalities are one of the largest groups of congenital abnormalities.
Musculoskeletal and limb abnormalities are one of the largest groups of congenital abnormalities.
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* '''Review - New Insights Into Cranial Synchondrosis Development'''{{#pmid:32850826|PMID32850826}} "The synchondroses formed via endochondral ossification in the cranial base are an important growth center for the neurocranium. Abnormalities in the synchondroses affect cranial base elongation and the development of adjacent regions, including the craniofacial bones. In the central region of the cranial base, there are two synchondroses present-the intersphenoid synchondrosis and the spheno-occipital synchondrosis. These synchondroses consist of mirror image bipolar growth plates. The cross-talk of several signaling pathways, including the parathyroid hormone-like hormone (PTHLH)/parathyroid hormone-related protein (PTHrP), Indian hedgehog (Ihh), Wnt/β-catenin, and fibroblast growth factor (FGF) pathways, as well as regulation by cilium assembly and the transcription factors encoded by the RUNX2, SIX1, SIX2, SIX4, and TBX1 genes, play critical roles in synchondrosis development. Deletions or activation of these gene products in mice causes the abnormal ossification of cranial synchondrosis and skeletal elements. Gene disruption leads to both similar and markedly different abnormalities in the development of intersphenoid synchondrosis and spheno-occipital synchondrosis, as well as in the phenotypes of synchondroses and skeletal bones. This paper reviews the development of cranial synchondroses, along with its regulation by the signaling pathways and transcription factors, highlighting the differences between intersphenoid synchondrosis and spheno-occipital synchondrosis."
* {{#pmid:32835424|PMID32835424}} '''YAP and TAZ promote periosteal osteoblast precursor expansion and differentiation for fracture repair''' "In response to bone fracture, periosteal progenitor cells proliferate, expand, and differentiate to form cartilage and bone in the fracture callus. These cellular functions require the coordinated activation of multiple transcriptional programs, and the transcriptional regulators Yes-associated protein (YAP) and transcriptional co-activator with PDZ-binding motif (TAZ) regulate osteochondroprogenitor activation during endochondral bone development. However, recent observations raise important distinctions between the signaling mechanisms used to control bone morphogenesis and repair. Here, we tested the hypothesis that YAP and TAZ regulate osteochondroprogenitor activation during endochondral bone fracture healing in mice. Constitutive YAP and/or TAZ deletion from Osterix-expressing cells impaired both cartilage callus formation and subsequent mineralization. However, this could be explained either by direct defects in osteochondroprogenitor differentiation after fracture, or by developmental deficiencies in the progenitor cell pool prior to fracture. Consistent with the second possibility, we found that developmental YAP/TAZ deletion produced long bones with impaired periosteal thickness and cellularity. Therefore, to remove the contributions of developmental history, we next generated adult onset-inducible knockout mice (using Osx-CretetOff ) in which YAP and TAZ were deleted prior to fracture, but after normal development."
* '''SHP2 regulates intramembranous ossification by modifying the TGFβ and BMP2 signaling pathway'''{{#pmid:30471432|PMID30471432}} "SHP2 is a ubiquitously expressed protein tyrosine phosphatase, which is involved in many signaling pathways to regulate the skeletal development. In endochondral ossification, SHP2 is known to modify the osteogenic fate of osteochondroprogenitors and to impair the osteoblastic transdifferentiation of hypertrophic chondrocytes. However, how SHP2 regulates osteoblast differentiation in intramembranous ossification remains incompletely understood. To address this question, we generated a mouse model to ablate SHP2 in the Prrx1-expressing mesenchymal progenitors by using "Cre-loxP"-mediated gene excision and examined the development of calvarial bone, in which the main process of bone formation is intramembranous ossification. Phenotypic characterization showed that SHP2 mutants have severe defects in calvarial bone formation. Cell lineage tracing and in situ hybridization data showed less osteoblast differentiation of mesenchymal cells and reduced osteogenic genes expression, respectively. Further mechanistic studies revealed enhanced TGFβ and suppressed BMP2 signaling in SHP2 ablated mesenchymal progenitors and their derivatives. Our study uncovered the critical role of SHP2 in osteoblast differentiation through intramembranous ossification and might provide a potential target to treat craniofacial skeleton disorders."
|}
|}


{| class="wikitable collapsible collapsed"
{| class="wikitable mw-collapsible mw-collapsed"
! More recent papers
! More recent papers  
|-
|-
| [[File:Mark_Hill.jpg|90px|left]] {{Most_Recent_Refs}}
| [[File:Mark_Hill.jpg|90px|left]] {{Most_Recent_Refs}}


Search term: ''Bone Embryology''
Search term: [http://www.ncbi.nlm.nih.gov/pubmed/?term=Bone+Embryology ''Bone Embryology''] | [http://www.ncbi.nlm.nih.gov/pubmed/?term=Bone+Development ''Bone Development''] | [http://www.ncbi.nlm.nih.gov/pubmed/?term=endochondral+ossification ''endochondral ossification''] | [http://www.ncbi.nlm.nih.gov/pubmed/?term=intramembranous+ossification ''intramembranous ossification''] | [http://www.ncbi.nlm.nih.gov/pubmed/?term=osteoblast ''osteoblast''] | [http://www.ncbi.nlm.nih.gov/pubmed/?term=osteoclast ''osteoclast'']
 
|}
{| class="wikitable mw-collapsible mw-collapsed"
! Older papers  
|-
| {{Older papers}}
* '''CT analysis of anatomical distribution of melorheostosis challenges the sclerotome hypothesis'''{{#pmid:30218789|PMID30218789}} "Melorheostosis (MEL) is a rare disease of high bone mass with patchy skeletal distribution affecting the long bones. We recently reported somatic mosaic mutations in MAP2K1 in 8 of 15 patients with the disease. The unique anatomic distribution of melorheostosis is of great interest. The disease remains limited to medial or lateral side of the extremity with proximo-distal progression. This pattern of distribution has historically been attributed to sclerotomes (area of bone which is innervated by a single spinal nerve level). ... This suggests that the mutation occurred after the formation of dorso-ventral plane. Further studies on limb development are needed to better understand the etiology, pathophysiology and pattern of disease distribution in all patients with MEL."
 
* '''Cell-matrix signals specify bone endothelial cells during developmental osteogenesis'''{{#pmid:28218908|PMID28218908}} “Blood vessels in the mammalian skeletal system control bone formation and support haematopoiesis by generating local niche environments. Here, we report that embryonic and early postnatal long bone contains a specialized endothelial cell subtype, termed type E, which strongly supports osteoblast lineage cells and later gives rise to other endothelial cell subpopulations. The differentiation and functional properties of bone endothelial cells require cell-matrix signalling interactions." [[Cardiovascular System - Blood Vessel Development|Blood Vessel Development]] | | [https://omim.org/entry/135630 INTEGRIN BETA-1]
 
* '''Disruption of Scube2 impairs endochondral bone formation'''{{#pmid:25639508|PMID25639508}} "SCUBE2 (signal peptide-CUB-EGF domain-containing protein 2) belongs to a secreted and membrane-tethered multi-domain SCUBE protein family composed of 3 members found in vertebrates and mammals. Recent reports suggested that zebrafish scube2 could facilitate sonic hedgehog (Shh) signaling for proper development of slow muscle. However, whether SCUBE2 can regulate the signaling activity of two other hedgehog ligands (Ihh and Dhh), and the developmental relevance of the SCUBE2-induced hedgehog signaling in mammals remain poorly understood. In this study, we first showed that as compared with SCUBE1 or 3, SCUBE2 is the most potent modulator of IHH signaling in vitro. In addition, gain and loss-of-function studies demonstrated that SCUBE2 exerted an osteogenic function by enhancing Ihh-stimulated osteoblast differentiation in the mouse mesenchymal progenitor cells." [[Developmental Signals - Sonic hedgehog|Sonic hedgehog]]


<pubmed limit=5>Bone Embryology</pubmed>
* '''Vascularization of primary and secondary ossification centres in the human growth plate'''{{#pmid:25164565|PMID25164565}}  "The switch from cartilage template to bone during endochondral ossification of the growth plate requires a dynamic and close interaction between cartilage and the developing vasculature. Vascular invasion of the primarily avascular hypertrophic chondrocyte zone brings chondroclasts, osteoblast- and endothelial precursor cells into future centres of ossification. ...Vascularization of ossification centres of the growth plate was mediated by sprouting of capillaries coming from the bone collar or by intussusception rather than by de-novo vessel formation involving endothelial progenitor cells. Vascular invasion of the joint anlage was temporally delayed compared to the surrounding joint tissue."
|}
|}
==Adult Human Skeleton==
==Adult Human Skeleton==
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{| class="prettytable" width=100%
{| class="prettytable" width=100%
|-
|-
| [[File:Mesoderm cartoon 01.jpg]]
| [[File:Mesoderm cartoon 01.jpg|200px]]
| [[File:Mesoderm cartoon 02.jpg|200px]]
| [[File:Mesoderm cartoon 03.jpg|200px]]
| [[File:Mesoderm cartoon 04.jpg|200px]]
|-
| Cells migrate through the primitive streak to form mesodermal layer. Extraembryonic mesoderm lies adjacent to the trilaminar embryo totally enclosing the amnion, yolk sac and forming the connecting stalk.
| Cells migrate through the primitive streak to form mesodermal layer. Extraembryonic mesoderm lies adjacent to the trilaminar embryo totally enclosing the amnion, yolk sac and forming the connecting stalk.
|-
| [[File:Mesoderm cartoon 02.jpg]]
| Paraxial mesoderm accumulates under the neural plate with thinner mesoderm laterally. This forms 2 thickened streaks running the length of the embryonic disc along the rostrocaudal axis. In humans, during the 3rd week, this mesoderm begins to segment. The neural plate folds to form a neural groove and folds.
| Paraxial mesoderm accumulates under the neural plate with thinner mesoderm laterally. This forms 2 thickened streaks running the length of the embryonic disc along the rostrocaudal axis. In humans, during the 3rd week, this mesoderm begins to segment. The neural plate folds to form a neural groove and folds.
|-
| Segmentation of the paraxial mesoderm into somites continues caudally at 1 somite/90minutes and a cavity (intraembryonic coelom) forms in the lateral plate mesoderm separating somatic and splanchnic mesoderm. Note intraembryonic coelomic cavity communicates with extraembryonic coelom through portals (holes) initially on lateral margin of embryonic disc.
| [[File:Mesoderm cartoon 03.jpg]]
| Segmentation of the paraxial mesoderm into somites continues caudally at 1 somite/90minutes and a cavity (intraembryonic coelom) forms in the lateral plate mesoderm separating somatic and splanchnic mesoderm.
 
Note intraembryonic coelomic cavity communicates with extraembryonic coelom through portals (holes) initially on lateral margin of embryonic disc.
|-
| [[File:Mesoderm cartoon 04.jpg]]
| Somites continue to form. The neural groove fuses dorsally to form a tube at the level of the 4th somite and "zips up cranially and caudally and the neural crest migrates into the mesoderm.
| Somites continue to form. The neural groove fuses dorsally to form a tube at the level of the 4th somite and "zips up cranially and caudally and the neural crest migrates into the mesoderm.
|-
|}
|}
=== Limb Development ===
=== Limb Development ===


{| class="prettytable" width=100%
{| class="prettytable" width=100%
|-
|-
| [[File:Mesoderm cartoon 09.jpg]]
| [[File:Somite_cartoon5.png]]
|
|
|-
|-
|}
|}
==Ossification Centres==
==Ossification Centres==
[[File:Mouse_limb_cartilage_and_bone_E14.5.jpg|thumb|300px|Mouse limb showing primary ossification.<ref><pubmed>20386744</pubmed>| [http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2851570 PMC2851570] | [http://www.plosgenetics.org/article/info%3Adoi%2F10.1371%2Fjournal.pgen.1000901 PLoS Genet.]</ref>]]
[[File:Mouse_limb_cartilage_and_bone_E14.5.jpg|thumb|300px|Mouse limb showing primary ossification.{{#pmid:20386744|PMID20386744}}]]
===Primary Ossification===
===Primary Ossification===
 
* Primary ossification centres are the first sites of bone formation and where cartilage has begun to degenerate. In long bones, this lis generally located mid-diaphysis (shaft). In other bones (e.g. base of skull) these are the initial locations of bone formation.
===Secondary Ossification===
===Secondary Ossification===
* Secondary ossification centres develop in the cartilage epiphysis of the long bones.
* Secondary ossification centres develop in the cartilage epiphysis of the long bones.
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** Used as a marker for term development if a secondary ossification centre present in either: head of femur, head of tibia, of head of humerus.  
** Used as a marker for term development if a secondary ossification centre present in either: head of femur, head of tibia, of head of humerus.  
** The last secondary centre to appear is the clavical medial epiphysis, that does not develop until 18 or 20 years.
** The last secondary centre to appear is the clavical medial epiphysis, that does not develop until 18 or 20 years.
==Endochondral Ossification==
Most of the bony skeleton forms by this process, that replaces a developmental cartilage template with bone.
{|
| colspan=6|Longitudinal views of endochondral bone formation in mouse limbs.{{#pmid:26893264|PMID26893264}}
|-
| colspan=6|[[File:Endochondral bone cartoon.jpg|100%]]
|-
|'''a''' - Prx1+ progenitors from lateral plate mesoderm proliferate to populate the emerging limb bud.
|'''b''' - Cells nearest the centre undergo mesenchymal condensation, express Col2a1 as they enter a chondrogenic differentiation program, and deposit a cartilage template.
|'''c''' to '''d''' -  Differentiating cells upregulate Col10a1 as they become hypertrophic, which triggers local formation of a bone collar and vascularization of the cartilage template. Invading blood vessels deliver an influx of haematopoietic cells that give rise to osteoclasts which excavate the cartilage template, and Osx1+ osteoblast progenitors and other blood cell types that populate the newly formed marrow cavity.
|'''d''' - A longitudinal growth axis is established when vascularization and osteoclast-mediated resorption bisect the presumptive skeletal element, producing two growth plates with opposing directionality. A perpendicular growth axis is driven by periosteal osteoblasts and allows the bone to grow in width.
| '''e''' - Within the remodelled cartilage template, bone-forming osteoblasts are derived from Osx1+ cells arriving with the invading vasculature, as well as hypertrophic Col10a1+ chondroctyes that transdifferentiate as they exit the growth plate into the marrow cavity. As bones grow in length and width, a second wave of vascularization forms the secondary ossification centres.
| '''f''' - Mature endochondral bone.
|}
See also [[Bone Histology]]
[[File:Endochondral ossification.jpg]] [[File:Endochondral ossification 2.jpg]]
[[File:Ossification_endochondral_1c.jpg|link=File:Ossification_endochondral_1.jpg]] [[File:Articular cartilage.jpg]]
:'''Links:''' [http://www.lab.anhb.uwa.edu.au/mb140/CorePages/Bone/Bone.htm#endochondral Blue Histology - endochondral] | [http://www.ncbi.nlm.nih.gov/bookshelf/br.fcgi?book=dbio&part=A3479&rendertype=figure&id=A3484 Dev Biology - endochondral ossification] | [http://commons.bcit.ca/biology/ossification/files/ossification2.html endochondral ossification animation]
==Intramembranous Ossification==
The process of {{intramembranous ossification}} (desmal ossification) occurs with mesenchyme directly ossifying into bone without a pre-existing cartilage template. Vascularised regions of mesenchymal cells{{#pmid:23737393|PMID23737393}} proliferate and differentiate into initially pre-osteoblasts and then osteoblasts, occurs in parts of the skull and the clavicle.
The vascularising endothelial cells promotes mesenchymal intramembranous ossification by {{BMP}} signaling pathway activated through Yap/Taz transcriptional activity.{{#pmid:27273480|PMID27273480}}
See also [[Bone Histology]]
[[File:Ossification_centre.jpg]] [[File:Intramembranous_ossification_centre.jpg]]
<gallery>
File:Bone histology 007.jpg|intramembranous - HE low
File:Bone histology 010.jpg|intramembranous - HE high
File:Bone histology 008.jpg|intramembranous - VG low
File:Bone histology 009.jpg|intramembranous - VG high
</gallery>
:'''Links:'''
===Human Fetal Head (12 week)===
[[File:Fetal head medial.jpg|300px]] [[File:Fetal head lateral.jpg|300px]]
[[File:Meckel.jpg|600px]]
[[File:Fetal_head_section.jpg|600px]]
==Bone Mineral Density==
Bone mineral density (BMD) is measured postnatally by an {{X-ray}} test and is generally used to detect bone loss associated with osteoporosis. The density of the total hip is a predictor for a hip fracture, while the lumbar spine is the site for monitoring the effect of clinical treatment.
A study of 18,713 individuals, has identified the postnatal male/female age at attainment of peak bone mineral density (BMD).{{#pmid:31760214|PMID31760214}}
{{Peak Bone Mineral Density Age table1}}
There are two main identified forms of osteoporosis:
# Primary osteoporosis - related to bone loss from ageing.
# Secondary osteoporosis - results from specific conditions that may be reversible.
:'''PubMed:''' [https://www.ncbi.nlm.nih.gov/pubmed/?term=Primary+osteoporosis Primary osteoporosis] | [https://www.ncbi.nlm.nih.gov/pubmed/?term=Secondary+osteoporosis Secondary osteoporosis] | [https://www.ncbi.nlm.nih.gov/pubmed/?term=Bone+mineral+density Bone mineral density]


==Bone Structure==
==Bone Structure==
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===Periosteum===
===Periosteum===
Connective tissue covering the surface of bone (except articular surfaces).
{|
| The embryonic origin of this layer is still controversial. The connective tissue coating covering the surface of bone, except at the articular surfaces, consisting of two distinct main layers with sub-layers.{{#pmid:20049593|PMID20049593}}
 
# '''outer fibrous layer'''
## outer - portion highly vascularized, cell poor with a predominant collagenous matrix and few elastic fibers.
## inner - portion not highly vascularized, cell poor with a fibroelastic layer, containing many elastic fibers.
# '''cambium layer''' - a highly cellular layer containing mesenchymal progenitor cells, differentiated osteogenic progenitor cells, osteoblasts and fibroblasts in a sparse collagenous matrix.
| [[File:Periosteum.jpg]]
|}


[[File:Periosteum.jpg]]


===Endosteum===
===Endosteum===
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* mature bone-forming cells embedded in '''lacunae''' within the bone matrix
* mature bone-forming cells embedded in '''lacunae''' within the bone matrix
* osteoblasts and osteocytes - secrete organic matrix of bone (osteoid), converted into '''osteocytes''' when become embedded in matrix (which calcifies soon after deposition)  
* osteoblasts and osteocytes - secrete organic matrix of bone (osteoid), converted into '''osteocytes''' when become embedded in matrix (which calcifies soon after deposition)  
===Osteoclasts===  
===Osteoclasts===  
[[File:Osteoclast.jpg]] [[File:Bone_remodeling_cycle.jpg|400px]]
[[File:Osteoclast.jpg]] [[File:Bone_remodeling_cycle.jpg|400px]]
Recently, Heterogeneous nuclear ribonucleoprotein K ([https://www.omim.org/entry/600712 hnRNPK]), a DNA/RNA-binding protein, has been shown to be essential for the formation of osteoclast.{{#pmid:31538344PMID31538344}}
* bone-resorbing multinucleated macrophage-like cells
* bone-resorbing multinucleated macrophage-like cells
* origin- fusion of monocytes or macrophages, Blood macrophage precursor, Attach to bone matrix
* originate by fusion of monocytes or macrophages, Blood macrophage precursor, Attach to bone matrix
* seal a small segment of extracellular space (between plasma membrane and bone surface), HCl and lysosomes secreted into this space by osteoclasts dissolves calcium phosphate crystals (give bone rigidity and strength)
* seal a small segment of extracellular space (between plasma membrane and bone surface), HCl and lysosomes secreted into this space by osteoclasts dissolves calcium phosphate crystals (give bone rigidity and strength)
** Resorptive bay - (Howship's lacuna) shallow bay lying directly under an osteoclast.
** Resorptive bay - (Howship's lacuna) shallow bay lying directly under an osteoclast.
* do not mistake for megakaryocytes, found in bone marrow not associated with bone matrix.
* do not mistake for megakaryocytes, found in bone marrow not associated with bone matrix.
** megakaryocytes are also multi-niucleated and form platelets
** megakaryocytes are also multi-niucleated and form platelets
:'''Links:''' [https://www.omim.org/entry/600712 OMIM - hnRNPK]
===Osteoclastogenesis===
* Formation of mature osteoclasts involved in bone resorption - the osteoblasts regulate this process through the production of RANKL (Receptor Activator for Nuclear Factor κ B Ligand) which is found on the cell surface of osteoblasts.
* RANKL is a key player in rheumatoid arthritis.
* Osteoclast origin - fusion of monocytes or macrophages, Blood macrophage precursor
* Attach to bone matrix - very large cells containing 15-20 nucleii.
* Lysosomes  - released into space between ruffled border and bone matrix, enzymes break down collagen fibres, resorption bays or Howship's lacunae
===Hippo===
Hippo signaling pathway has recently been identified as a regulator of osteoclast formation (see review{{#pmid:29219182|PMID29219182}}
* Hippo signaling pathway regulatory molecules - RASSF2, NF2, MST1/2, SAV1, LATS1/2, MOB1, YAP, and TAZ.
* osteoclast differentiation - upon activation, MST and LAST, transcriptional co-activators YAP and TAZ bind to the members of the TEA domain (TEAD) family transcription factors
** regulate expression of downstream target genes connective tissue growth factor (CTGF/CCN2) and cysteine-rich protein 61 (CYR61/CCN1).
* RANKL-mediated signaling cascades including NF-κB, MAPKs, AP1, and NFATc1, Hippo-signaling molecules such as YAP/TAZ/TEAD complex, RASSF2, MST2, and Ajuba could also potentially modulate osteoclast differentiation and function.
:'''Links:''' {{Hippo}}


===Bone Marrow===
===Bone Marrow===
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* '''stromal cells''' - all other support cells not involved in haematopoiesis
* '''stromal cells''' - all other support cells not involved in haematopoiesis


:'''Links:''' [[Cardiovascular_System_-_Blood_Development|Blood Development]]
 
:'''Links:''' {{Blood}}


Marrow stroma components:
Marrow stroma components:
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* osteocytes extending cytoplasmic processes into canaliculi
* osteocytes extending cytoplasmic processes into canaliculi
* Additional Histology images: [http://embryology.med.unsw.edu.au/pdf/histoimages/10ax10.pdf low] | [http://embryology.med.unsw.edu.au/pdf/histoimages/10ax20.pdf medium] | [http://embryology.med.unsw.edu.au/pdf/histoimages/10ax40.pdf high]
* Additional Histology images: [http://embryology.med.unsw.edu.au/pdf/histoimages/10ax10.pdf low] | [http://embryology.med.unsw.edu.au/pdf/histoimages/10ax20.pdf medium] | [http://embryology.med.unsw.edu.au/pdf/histoimages/10ax40.pdf high]
==Endochondral Ossification==
[[File:Endochondral_bone.jpg|thumb]]
See also [[Bone Histology]]


[[File:Endochondral ossification.jpg]] [[File:Endochondral ossification 2.jpg]]
[[File:Ossification_endochondral_1c.jpg|link=File:Ossification_endochondral_1.jpg]] [[File:Articular cartilage.jpg]]
:'''Links:''' [http://www.lab.anhb.uwa.edu.au/mb140/CorePages/Bone/Bone.htm#endochondral Blue Histology - endochondral] | [http://www.ncbi.nlm.nih.gov/bookshelf/br.fcgi?book=dbio&part=A3479&rendertype=figure&id=A3484 Dev Biology - endochondral ossification] | [http://commons.bcit.ca/biology/ossification/files/ossification2.html endochondral ossification animation]
==Intramembranous Ossification==
See also [[Bone Histology]]
[[File:Ossification_centre.jpg]] [[File:Intramembranous_ossification_centre.jpg]]
:'''Links:''' [http://www.lab.anhb.uwa.edu.au/mb140/CorePages/Bone/Bone.htm#intramembranous Blue histology - intramembranous] | [http://commons.bcit.ca/biology/ossification/files/ossification1.html intramembranous ossification animation]
===Human Fetal Head (12 week)===
[[File:Fetal head medial.jpg|300px]] [[File:Fetal head lateral.jpg|300px]]
[[File:Meckel.jpg|600px]]
[[File:Fetal_head_section.jpg|600px]]


==Molecular==
==Molecular==
[[File:Cleidocranial dysplasia 01.jpg|thumb|alt=Cleidocranial dysplasia CT and Xray|Cleidocranial dysplasia associated with RUNX2 mutation.{{#pmid:28173761|PMID28173761}}]]
The transcription factors Runx2 and Runx3 are essential for chondrocyte maturation, while Runx2 and Osterix are essential for osteoblast differentiation.
The transcription factors Runx2 and Runx3 are essential for chondrocyte maturation, while Runx2 and Osterix are essential for osteoblast differentiation.


===Osterix===
===Osterix===
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===Osteogenesis Imperfecta===
===Osteogenesis Imperfecta===


Osteogenesis Imperfecta (OI,  brittle bone disease) originally described as a collagen 1 gene mutation, but can have several different genetic causes and can be classified into eight different types (I-VIII).<ref><pubmed>19907330</pubmed></ref>
Osteogenesis Imperfecta (OI,  brittle bone disease) originally described as a collagen 1 gene mutation, but can have several different genetic causes and can be classified into eight different types (I-VIII).{{#pmid:19907330|PMID19907330}}


* COL1A1 and COL1A2 mutations
* COL1A1 and COL1A2 mutations
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===Reviews===
===Reviews===
<pubmed>19883365</pubmed>
32850793
<pubmed>17659995</pubmed>
 
{{#pmid:28786518}}
 
{{#pmid:26453494}}
 
{{#pmid:23794334}}
 
{{#pmid:23284041}}
 
{{#pmid:20049593}}
 
{{#pmid:19883365}}
 
{{#pmid:17659995}}


===Articles===  
===Articles===  
===Journals===
* [http://www.journals.elsevier.com/bone BONE] is an interdisciplinary forum for the rapid publication of original articles and reviews on basic, translational, and clinical aspects of bone and mineral metabolism.


===Search PubMed===
===Search PubMed===
Search April 2010
* Musculoskeletal System Development - All (44637) Review (5065) Free Full Text (6601)
* Musculoskeletal Development - All (44637) Review (5065) Free Full Text (6601)


'''Search Pubmed:''' [http://www.ncbi.nlm.nih.gov/sites/entrez?db=pubmed&cmd=search&term=Bone%20Development Bone Development] | [http://www.ncbi.nlm.nih.gov/sites/entrez?db=pubmed&cmd=search&term=developmental%20ossification developmental ossification] | [http://www.ncbi.nlm.nih.gov/sites/entrez?db=pubmed&cmd=search&term=endochondral%20ossification endochondral ossification] | [http://www.ncbi.nlm.nih.gov/sites/entrez?db=pubmed&cmd=search&term=intramembranous%20ossification intramembranous ossification]
'''Search Pubmed:''' [http://www.ncbi.nlm.nih.gov/sites/entrez?db=pubmed&cmd=search&term=Bone%20Development Bone Development] | [http://www.ncbi.nlm.nih.gov/sites/entrez?db=pubmed&cmd=search&term=developmental%20ossification developmental ossification] | [http://www.ncbi.nlm.nih.gov/sites/entrez?db=pubmed&cmd=search&term=endochondral%20ossification endochondral ossification] | [http://www.ncbi.nlm.nih.gov/sites/entrez?db=pubmed&cmd=search&term=intramembranous%20ossification intramembranous ossification]
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File:Fetal_head_section.jpg|Fetal head section (12 weeks)
File:Fetal_head_section.jpg|Fetal head section (12 weeks)
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==Terms==
{{Bone terms}}


== External Links ==
== External Links ==
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[[Category:System Development]][[Category:Bone]]
[[Category:System Development]]

Latest revision as of 14:19, 28 August 2020

Embryology - 19 Mar 2024    Facebook link Pinterest link Twitter link  Expand to Translate  
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Introduction

Endochondral bone
Bone femur diagram
Bone femur

The skeleton consists of bone developing from mesoderm, except within the head where neural crest also contributes connective tissues. Each tissue (cartilage, bone, and skeletal muscle) goes through many different mechanisms of differentiation.


The 2 key developmental processes are the initial "patterning" of bone location and then the overt "differentiation" of bone through the process of ossification. For details on specific bone differentiation in human development see Bone Development Timeline.


Bone is formed through a lengthy process involving ossification of a cartilage formed from mesenchyme. Two main forms of ossification occur in different bones, intramembranous ossification (eg skull) and endochondral ossification (eg limb long bones) ossification. Ossification continues postnatally, through puberty until mid 20's. Early ossification occurs at the ends of long bones. A study of 18,713 individuals identified the male/female age at attainment of peak bone mineral density (BMD).[1]


The two major parts of the human skeleton are the axial (80 bones in skull, vertebra, ribs, sternum) and appendicular (126 bones in limbs, shoulders, pelvis) skeletons.


Musculoskeletal and limb abnormalities are one of the largest groups of congenital abnormalities.


Musculoskeletal Links: Introduction | mesoderm | somitogenesis | limb | cartilage | bone | bone timeline | bone marrow | shoulder | pelvis | axial skeleton | skull | joint | skeletal muscle | muscle timeline | tendon | diaphragm | Lecture - Musculoskeletal | Lecture Movie | musculoskeletal abnormalities | limb abnormalities | developmental hip dysplasia | cartilage histology | bone histology | Skeletal Muscle Histology | Category:Musculoskeletal
Historic Embryology - Musculoskeletal  
1853 Bone | 1885 Sphenoid | 1902 - Pubo-femoral Region | Spinal Column and Back | Body Segmentation | Cranium | Body Wall, Ribs, and Sternum | Limbs | 1901 - Limbs | 1902 - Arm Development | 1906 Human Embryo Ossification | 1906 Lower limb Nerves and Muscle | 1907 - Muscular System | Skeleton and Limbs | 1908 Vertebra | 1908 Cervical Vertebra | 1909 Mandible | 1910 - Skeleton and Connective Tissues | Muscular System | Coelom and Diaphragm | 1913 Clavicle | 1920 Clavicle | 1921 - External body form | Connective tissues and skeletal | Muscular | Diaphragm | 1929 Rat Somite | 1932 Pelvis | 1940 Synovial Joints | 1943 Human Embryonic, Fetal and Circumnatal Skeleton | 1947 Joints | 1949 Cartilage and Bone | 1957 Chondrification Hands and Feet | 1968 Knee

Some Recent Findings

Historic images of the skull by Vesalius
  • Review - New Insights Into Cranial Synchondrosis Development[2] "The synchondroses formed via endochondral ossification in the cranial base are an important growth center for the neurocranium. Abnormalities in the synchondroses affect cranial base elongation and the development of adjacent regions, including the craniofacial bones. In the central region of the cranial base, there are two synchondroses present-the intersphenoid synchondrosis and the spheno-occipital synchondrosis. These synchondroses consist of mirror image bipolar growth plates. The cross-talk of several signaling pathways, including the parathyroid hormone-like hormone (PTHLH)/parathyroid hormone-related protein (PTHrP), Indian hedgehog (Ihh), Wnt/β-catenin, and fibroblast growth factor (FGF) pathways, as well as regulation by cilium assembly and the transcription factors encoded by the RUNX2, SIX1, SIX2, SIX4, and TBX1 genes, play critical roles in synchondrosis development. Deletions or activation of these gene products in mice causes the abnormal ossification of cranial synchondrosis and skeletal elements. Gene disruption leads to both similar and markedly different abnormalities in the development of intersphenoid synchondrosis and spheno-occipital synchondrosis, as well as in the phenotypes of synchondroses and skeletal bones. This paper reviews the development of cranial synchondroses, along with its regulation by the signaling pathways and transcription factors, highlighting the differences between intersphenoid synchondrosis and spheno-occipital synchondrosis."
  • [3] YAP and TAZ promote periosteal osteoblast precursor expansion and differentiation for fracture repair "In response to bone fracture, periosteal progenitor cells proliferate, expand, and differentiate to form cartilage and bone in the fracture callus. These cellular functions require the coordinated activation of multiple transcriptional programs, and the transcriptional regulators Yes-associated protein (YAP) and transcriptional co-activator with PDZ-binding motif (TAZ) regulate osteochondroprogenitor activation during endochondral bone development. However, recent observations raise important distinctions between the signaling mechanisms used to control bone morphogenesis and repair. Here, we tested the hypothesis that YAP and TAZ regulate osteochondroprogenitor activation during endochondral bone fracture healing in mice. Constitutive YAP and/or TAZ deletion from Osterix-expressing cells impaired both cartilage callus formation and subsequent mineralization. However, this could be explained either by direct defects in osteochondroprogenitor differentiation after fracture, or by developmental deficiencies in the progenitor cell pool prior to fracture. Consistent with the second possibility, we found that developmental YAP/TAZ deletion produced long bones with impaired periosteal thickness and cellularity. Therefore, to remove the contributions of developmental history, we next generated adult onset-inducible knockout mice (using Osx-CretetOff ) in which YAP and TAZ were deleted prior to fracture, but after normal development."
  • SHP2 regulates intramembranous ossification by modifying the TGFβ and BMP2 signaling pathway[4] "SHP2 is a ubiquitously expressed protein tyrosine phosphatase, which is involved in many signaling pathways to regulate the skeletal development. In endochondral ossification, SHP2 is known to modify the osteogenic fate of osteochondroprogenitors and to impair the osteoblastic transdifferentiation of hypertrophic chondrocytes. However, how SHP2 regulates osteoblast differentiation in intramembranous ossification remains incompletely understood. To address this question, we generated a mouse model to ablate SHP2 in the Prrx1-expressing mesenchymal progenitors by using "Cre-loxP"-mediated gene excision and examined the development of calvarial bone, in which the main process of bone formation is intramembranous ossification. Phenotypic characterization showed that SHP2 mutants have severe defects in calvarial bone formation. Cell lineage tracing and in situ hybridization data showed less osteoblast differentiation of mesenchymal cells and reduced osteogenic genes expression, respectively. Further mechanistic studies revealed enhanced TGFβ and suppressed BMP2 signaling in SHP2 ablated mesenchymal progenitors and their derivatives. Our study uncovered the critical role of SHP2 in osteoblast differentiation through intramembranous ossification and might provide a potential target to treat craniofacial skeleton disorders."
More recent papers  
Mark Hill.jpg
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This table allows an automated computer search of the external PubMed database using the listed "Search term" text link.

  • This search now requires a manual link as the original PubMed extension has been disabled.
  • The displayed list of references do not reflect any editorial selection of material based on content or relevance.
  • References also appear on this list based upon the date of the actual page viewing.


References listed on the rest of the content page and the associated discussion page (listed under the publication year sub-headings) do include some editorial selection based upon both relevance and availability.

More? References | Discussion Page | Journal Searches | 2019 References | 2020 References

Search term: Bone Embryology | Bone Development | endochondral ossification | intramembranous ossification | osteoblast | osteoclast

Older papers  
These papers originally appeared in the Some Recent Findings table, but as that list grew in length have now been shuffled down to this collapsible table.

See also the Discussion Page for other references listed by year and References on this current page.

  • CT analysis of anatomical distribution of melorheostosis challenges the sclerotome hypothesis[5] "Melorheostosis (MEL) is a rare disease of high bone mass with patchy skeletal distribution affecting the long bones. We recently reported somatic mosaic mutations in MAP2K1 in 8 of 15 patients with the disease. The unique anatomic distribution of melorheostosis is of great interest. The disease remains limited to medial or lateral side of the extremity with proximo-distal progression. This pattern of distribution has historically been attributed to sclerotomes (area of bone which is innervated by a single spinal nerve level). ... This suggests that the mutation occurred after the formation of dorso-ventral plane. Further studies on limb development are needed to better understand the etiology, pathophysiology and pattern of disease distribution in all patients with MEL."
  • Cell-matrix signals specify bone endothelial cells during developmental osteogenesis[6] “Blood vessels in the mammalian skeletal system control bone formation and support haematopoiesis by generating local niche environments. Here, we report that embryonic and early postnatal long bone contains a specialized endothelial cell subtype, termed type E, which strongly supports osteoblast lineage cells and later gives rise to other endothelial cell subpopulations. The differentiation and functional properties of bone endothelial cells require cell-matrix signalling interactions." Blood Vessel Development | | INTEGRIN BETA-1
  • Disruption of Scube2 impairs endochondral bone formation[7] "SCUBE2 (signal peptide-CUB-EGF domain-containing protein 2) belongs to a secreted and membrane-tethered multi-domain SCUBE protein family composed of 3 members found in vertebrates and mammals. Recent reports suggested that zebrafish scube2 could facilitate sonic hedgehog (Shh) signaling for proper development of slow muscle. However, whether SCUBE2 can regulate the signaling activity of two other hedgehog ligands (Ihh and Dhh), and the developmental relevance of the SCUBE2-induced hedgehog signaling in mammals remain poorly understood. In this study, we first showed that as compared with SCUBE1 or 3, SCUBE2 is the most potent modulator of IHH signaling in vitro. In addition, gain and loss-of-function studies demonstrated that SCUBE2 exerted an osteogenic function by enhancing Ihh-stimulated osteoblast differentiation in the mouse mesenchymal progenitor cells." Sonic hedgehog
  • Vascularization of primary and secondary ossification centres in the human growth plate[8] "The switch from cartilage template to bone during endochondral ossification of the growth plate requires a dynamic and close interaction between cartilage and the developing vasculature. Vascular invasion of the primarily avascular hypertrophic chondrocyte zone brings chondroclasts, osteoblast- and endothelial precursor cells into future centres of ossification. ...Vascularization of ossification centres of the growth plate was mediated by sprouting of capillaries coming from the bone collar or by intussusception rather than by de-novo vessel formation involving endothelial progenitor cells. Vascular invasion of the joint anlage was temporally delayed compared to the surrounding joint tissue."

Adult Human Skeleton

Adult axial skeleton
Adult appendicular skeleton
Adult axial skeleton Adult appendicular skeleton

Textbooks

Fetal head (week 12)
  • The Developing Human: Clinically Oriented Embryology (8th Edition) by Keith L. Moore and T.V.N Persaud - Moore & Persaud Chapter 15 the skeletal system
  • Larsen’s Human Embryology by GC. Schoenwolf, SB. Bleyl, PR. Brauer and PH. Francis-West - Chapter 11 Limb Dev (bone not well covered in this textbook)
  • Before we Are Born (5th ed.) Moore and Persaud Chapter 16,17: p379-397, 399-405
  • Essentials of Human Embryology Larson Chapter 11 p207-228

Objectives

  • Identify the components of a somite and the adult derivatives of each component.
  • Give examples of sites of (a) endochondral and (b) intramembranous ossification and to compare these two processes.
  • Identify the general times (a) of formation of primary and (b) of formation of secondary ossification centres, and (c) of fusion of such centres with each other.
  • Briefly summarise the development of the limbs.
  • Describe the developmental abnormalities responsible for the following malformations: selected growth plate disorders; congenital dislocation of the hip; scoliosis; arthrogryposis; and limb reduction deformities.

Development Overview

Below is a very brief overview using simple figures of 3 aspects of early musculoskeletal development. More detailed overviews are shown on other notes pages Mesoderm and Somite, Vertebral Column, Limb in combination with serial sections and Carnegie images.

Mesoderm Development

Mesoderm cartoon 01.jpg Mesoderm cartoon 02.jpg Mesoderm cartoon 03.jpg Mesoderm cartoon 04.jpg
Cells migrate through the primitive streak to form mesodermal layer. Extraembryonic mesoderm lies adjacent to the trilaminar embryo totally enclosing the amnion, yolk sac and forming the connecting stalk. Paraxial mesoderm accumulates under the neural plate with thinner mesoderm laterally. This forms 2 thickened streaks running the length of the embryonic disc along the rostrocaudal axis. In humans, during the 3rd week, this mesoderm begins to segment. The neural plate folds to form a neural groove and folds. Segmentation of the paraxial mesoderm into somites continues caudally at 1 somite/90minutes and a cavity (intraembryonic coelom) forms in the lateral plate mesoderm separating somatic and splanchnic mesoderm. Note intraembryonic coelomic cavity communicates with extraembryonic coelom through portals (holes) initially on lateral margin of embryonic disc. Somites continue to form. The neural groove fuses dorsally to form a tube at the level of the 4th somite and "zips up cranially and caudally and the neural crest migrates into the mesoderm.

Limb Development

Somite cartoon5.png

Ossification Centres

Mouse limb showing primary ossification.[9]

Primary Ossification

  • Primary ossification centres are the first sites of bone formation and where cartilage has begun to degenerate. In long bones, this lis generally located mid-diaphysis (shaft). In other bones (e.g. base of skull) these are the initial locations of bone formation.

Secondary Ossification

  • Secondary ossification centres develop in the cartilage epiphysis of the long bones.
  • No medulary cavity forms in a secondary ossification center.
  • Appears late in fetal development.
    • Used as a marker for term development if a secondary ossification centre present in either: head of femur, head of tibia, of head of humerus.
    • The last secondary centre to appear is the clavical medial epiphysis, that does not develop until 18 or 20 years.

Endochondral Ossification

Most of the bony skeleton forms by this process, that replaces a developmental cartilage template with bone.

Longitudinal views of endochondral bone formation in mouse limbs.[10]
100%
a - Prx1+ progenitors from lateral plate mesoderm proliferate to populate the emerging limb bud. b - Cells nearest the centre undergo mesenchymal condensation, express Col2a1 as they enter a chondrogenic differentiation program, and deposit a cartilage template. c to d - Differentiating cells upregulate Col10a1 as they become hypertrophic, which triggers local formation of a bone collar and vascularization of the cartilage template. Invading blood vessels deliver an influx of haematopoietic cells that give rise to osteoclasts which excavate the cartilage template, and Osx1+ osteoblast progenitors and other blood cell types that populate the newly formed marrow cavity. d - A longitudinal growth axis is established when vascularization and osteoclast-mediated resorption bisect the presumptive skeletal element, producing two growth plates with opposing directionality. A perpendicular growth axis is driven by periosteal osteoblasts and allows the bone to grow in width. e - Within the remodelled cartilage template, bone-forming osteoblasts are derived from Osx1+ cells arriving with the invading vasculature, as well as hypertrophic Col10a1+ chondroctyes that transdifferentiate as they exit the growth plate into the marrow cavity. As bones grow in length and width, a second wave of vascularization forms the secondary ossification centres. f - Mature endochondral bone.


See also Bone Histology


Endochondral ossification.jpg Endochondral ossification 2.jpg

Ossification endochondral 1c.jpg Articular cartilage.jpg


Links: Blue Histology - endochondral | Dev Biology - endochondral ossification | endochondral ossification animation

Intramembranous Ossification

The process of intramembranous ossification (desmal ossification) occurs with mesenchyme directly ossifying into bone without a pre-existing cartilage template. Vascularised regions of mesenchymal cells[11] proliferate and differentiate into initially pre-osteoblasts and then osteoblasts, occurs in parts of the skull and the clavicle.

The vascularising endothelial cells promotes mesenchymal intramembranous ossification by BMP signaling pathway activated through Yap/Taz transcriptional activity.[12]


See also Bone Histology

Ossification centre.jpg Intramembranous ossification centre.jpg



Links:

Human Fetal Head (12 week)

Fetal head medial.jpg Fetal head lateral.jpg

Meckel.jpg

Fetal head section.jpg

Bone Mineral Density

Bone mineral density (BMD) is measured postnatally by an X-ray test and is generally used to detect bone loss associated with osteoporosis. The density of the total hip is a predictor for a hip fracture, while the lumbar spine is the site for monitoring the effect of clinical treatment.

A study of 18,713 individuals, has identified the postnatal male/female age at attainment of peak bone mineral density (BMD).[1]

Age (years) of Peak Bone Mineral Density (BMD)
femoral neck total hip lumbar spine
Male 20.5 21.2 23 .6
Female 18.7 19.0 20.1
Table based on data from[1] {More? bone)


There are two main identified forms of osteoporosis:

  1. Primary osteoporosis - related to bone loss from ageing.
  2. Secondary osteoporosis - results from specific conditions that may be reversible.
PubMed: Primary osteoporosis | Secondary osteoporosis | Bone mineral density

Bone Structure

Terminology

  • Diaphysis - shaft
  • Epiphysis - expanded ends
  • Metaphysis - connecting region (between diaphysis and epiphysial line)
  • Medullary Cavity - (marrow) cavity within the bone. Can also be identified as either red or yellow marrow.
Bone-femur.jpg

Compact bone

  • (dense) no spaces or hollows in the bone matrix visible to the eye.
  • forms the thick-walled tube of the shaft (or diaphysis) of long bones, which surrounds the marrow cavity (or medullary cavity). A thin layer of compact bone also covers the epiphyses of long bones.

Trabecular bone

  • (cancellous or spongy bone) consists of delicate bars (spicules) and sheets of bone, trabeculae
  • branch and intersect to form a sponge-like network
  • ends of long bones (or epiphyses) consist mainly of trabecular bone.

Periosteum

The embryonic origin of this layer is still controversial. The connective tissue coating covering the surface of bone, except at the articular surfaces, consisting of two distinct main layers with sub-layers.[13]
  1. outer fibrous layer
    1. outer - portion highly vascularized, cell poor with a predominant collagenous matrix and few elastic fibers.
    2. inner - portion not highly vascularized, cell poor with a fibroelastic layer, containing many elastic fibers.
  2. cambium layer - a highly cellular layer containing mesenchymal progenitor cells, differentiated osteogenic progenitor cells, osteoblasts and fibroblasts in a sparse collagenous matrix.
Periosteum.jpg


Endosteum

Connective tissue lining inner surface of bone.


Bone Growth

  • Appositional growth occurs at either the periosteum (outer surface), or the endosteum (inner surface).
  • Osteoblasts secrete osteoid, a pre-bone material composed mainly of type I collagen that becomes mineralized.
  • Early bone matrix deposited in development and during repair is woven rather than lamellar in appearance and structure.
  • In development, there are 2 distinct types of bone formation (intramembranous and endochondral)

Bone Cells

Osteoblasts

  • derive from osteogenic stem cells the osteoprogenitor cells that differentiate to form pre-osteoblast then osteoblasts maturing to an osteocyte
  • osteoprogenitor cells - "resting cell" line the inner and outer surfaces of bone

Osteocytes

  • mature bone-forming cells embedded in lacunae within the bone matrix
  • osteoblasts and osteocytes - secrete organic matrix of bone (osteoid), converted into osteocytes when become embedded in matrix (which calcifies soon after deposition)

Osteoclasts

Osteoclast.jpg Bone remodeling cycle.jpg

Recently, Heterogeneous nuclear ribonucleoprotein K (hnRNPK), a DNA/RNA-binding protein, has been shown to be essential for the formation of osteoclast.PubmedParser error: Invalid PMID, please check. (PMID: 31538344PMID31538344)


  • bone-resorbing multinucleated macrophage-like cells
  • originate by fusion of monocytes or macrophages, Blood macrophage precursor, Attach to bone matrix
  • seal a small segment of extracellular space (between plasma membrane and bone surface), HCl and lysosomes secreted into this space by osteoclasts dissolves calcium phosphate crystals (give bone rigidity and strength)
    • Resorptive bay - (Howship's lacuna) shallow bay lying directly under an osteoclast.
  • do not mistake for megakaryocytes, found in bone marrow not associated with bone matrix.
    • megakaryocytes are also multi-niucleated and form platelets
Links: OMIM - hnRNPK

Osteoclastogenesis

  • Formation of mature osteoclasts involved in bone resorption - the osteoblasts regulate this process through the production of RANKL (Receptor Activator for Nuclear Factor κ B Ligand) which is found on the cell surface of osteoblasts.
  • RANKL is a key player in rheumatoid arthritis.
  • Osteoclast origin - fusion of monocytes or macrophages, Blood macrophage precursor
  • Attach to bone matrix - very large cells containing 15-20 nucleii.
  • Lysosomes - released into space between ruffled border and bone matrix, enzymes break down collagen fibres, resorption bays or Howship's lacunae

Hippo

Hippo signaling pathway has recently been identified as a regulator of osteoclast formation (see review[14]

  • Hippo signaling pathway regulatory molecules - RASSF2, NF2, MST1/2, SAV1, LATS1/2, MOB1, YAP, and TAZ.
  • osteoclast differentiation - upon activation, MST and LAST, transcriptional co-activators YAP and TAZ bind to the members of the TEA domain (TEAD) family transcription factors
    • regulate expression of downstream target genes connective tissue growth factor (CTGF/CCN2) and cysteine-rich protein 61 (CYR61/CCN1).
  • RANKL-mediated signaling cascades including NF-κB, MAPKs, AP1, and NFATc1, Hippo-signaling molecules such as YAP/TAZ/TEAD complex, RASSF2, MST2, and Ajuba could also potentially modulate osteoclast differentiation and function.


Links: Hippo

Bone Marrow

Hematopoietic and stromal cell differentiation
  • red marrow - mainly haematopoietic (myeloid) tissue, newborn has all red marrow
  • yellow marrow - mainly fat cells, found in diaphysis region of long bones
  • stromal cells - all other support cells not involved in haematopoiesis


Links: blood

Marrow stroma components:

  1. osteoblasts - enclose the marrow compartment in bone tissue.
  2. endothelial and smooth muscle cells - organized into a complex vascular network composed of arterioles, capillaries, sinusoids, and a large central vein.
  3. nerves - sensory and sympathetic nerve fibres, glia, and perineural cells that innervate the marrow compartment to form a neural network.
  4. adipocytes - support metabolic functions of the bone marrow.
  5. stromal cells - support haematopoiesis and retain skeletal potential.

Bone Matrix

The bone matrix has 2 major components.

  • Organic portion composed of mainly collagen Type 1 (about 95%) and amorphous ground substance.
  • Inorganic portion (50% dry weight of the matrix) composed of hydroxyapatite crystals, calcium, phosphorus, bicarbonate, nitrate, Mg, K, Na.
    • storage calcium and phosphate
    • regulate blood calcium levels

Haversian Systems

Bone structure cartoon
  • also called osteons
  • Volkmann's canals - interconnect Haversian systems

Lamellae

  • concentric - surrounding each Haversian System
  • interstitial - bony plates that fill in between the haversian systems.
  • circumferential - layers of bone that underlie the periosteum and endosteum

Cells

  • osteocytes extending cytoplasmic processes into canaliculi
  • Additional Histology images: low | medium | high


Molecular

Cleidocranial dysplasia CT and Xray
Cleidocranial dysplasia associated with RUNX2 mutation.[15]

The transcription factors Runx2 and Runx3 are essential for chondrocyte maturation, while Runx2 and Osterix are essential for osteoblast differentiation.


Osterix

Osterix (OSX) encodes a transcription factor containing three Cys2-His2 zinc-finger DNA-binding domains at its C terminus that has been shown to be essential for bone formation.

Abnormalities

Osteogenesis Imperfecta

Osteogenesis Imperfecta (OI, brittle bone disease) originally described as a collagen 1 gene mutation, but can have several different genetic causes and can be classified into eight different types (I-VIII).[16]

  • COL1A1 and COL1A2 mutations
  • CRTAP and LEPRE1 mutations, in severe/lethal and recessively inherited osteogenesis imperfecta


Links: Musculoskeletal Abnormalities

References

  1. 1.0 1.1 1.2 Xue S, Kemal O, Lu M, Lix LM, Leslie WD & Yang S. (2020). Age at attainment of peak bone mineral density and its associated factors: The National Health and Nutrition Examination Survey 2005-2014. Bone , 131, 115163. PMID: 31760214 DOI.
  2. Funato N. (2020). New Insights Into Cranial Synchondrosis Development: A Mini Review. Front Cell Dev Biol , 8, 706. PMID: 32850826 DOI.
  3. Kegelman CD, Nijsure MP, Moharrer Y, Pearson HB, Dawahare JH, Jordan KM, Qin L & Boerckel JD. (2020). YAP and TAZ promote periosteal osteoblast precursor expansion and differentiation for fracture repair. J. Bone Miner. Res. , , . PMID: 32835424 DOI.
  4. Wang L, Huang J, Moore DC, Song Y, Ehrlich MG & Yang W. (2019). SHP2 regulates intramembranous ossification by modifying the TGFβ and BMP2 signaling pathway. Bone , 120, 327-335. PMID: 30471432 DOI.
  5. Jha S, Laucis N, Kim L, Malayeri A, Dasgupta A, Papadakis GZ, Karantanas A, Torres M & Bhattacharyya T. (2018). CT analysis of anatomical distribution of melorheostosis challenges the sclerotome hypothesis. Bone , 117, 31-36. PMID: 30218789 DOI.
  6. Langen UH, Pitulescu ME, Kim JM, Enriquez-Gasca R, Sivaraj KK, Kusumbe AP, Singh A, Di Russo J, Bixel MG, Zhou B, Sorokin L, Vaquerizas JM & Adams RH. (2017). Cell-matrix signals specify bone endothelial cells during developmental osteogenesis. Nat. Cell Biol. , 19, 189-201. PMID: 28218908 DOI.
  7. Lin YC, Roffler SR, Yan YT & Yang RB. (2015). Disruption of Scube2 Impairs Endochondral Bone Formation. J. Bone Miner. Res. , 30, 1255-67. PMID: 25639508 DOI.
  8. Walzer SM, Cetin E, Grübl-Barabas R, Sulzbacher I, Rueger B, Girsch W, Toegel S, Windhager R & Fischer MB. (2014). Vascularization of primary and secondary ossification centres in the human growth plate. BMC Dev. Biol. , 14, 36. PMID: 25164565 DOI.
  9. Galli A, Robay D, Osterwalder M, Bao X, Bénazet JD, Tariq M, Paro R, Mackem S & Zeller R. (2010). Distinct roles of Hand2 in initiating polarity and posterior Shh expression during the onset of mouse limb bud development. PLoS Genet. , 6, e1000901. PMID: 20386744 DOI.
  10. Salazar VS, Gamer LW & Rosen V. (2016). BMP signalling in skeletal development, disease and repair. Nat Rev Endocrinol , 12, 203-21. PMID: 26893264 DOI.
  11. Percival CJ & Richtsmeier JT. (2013). Angiogenesis and intramembranous osteogenesis. Dev. Dyn. , 242, 909-22. PMID: 23737393 DOI.
  12. Uemura M, Nagasawa A & Terai K. (2016). Yap/Taz transcriptional activity in endothelial cells promotes intramembranous ossification via the BMP pathway. Sci Rep , 6, 27473. PMID: 27273480 DOI.
  13. Dwek JR. (2010). The periosteum: what is it, where is it, and what mimics it in its absence?. Skeletal Radiol. , 39, 319-23. PMID: 20049593 DOI.
  14. Yang W, Han W, Qin A, Wang Z, Xu J & Qian Y. (2018). The emerging role of Hippo signaling pathway in regulating osteoclast formation. J. Cell. Physiol. , 233, 4606-4617. PMID: 29219182 DOI.
  15. Xu W, Chen Q, Liu C, Chen J, Xiong F & Wu B. (2017). A novel, complex RUNX2 gene mutation causes cleidocranial dysplasia. BMC Med. Genet. , 18, 13. PMID: 28173761 DOI.
  16. Shapiro JR & Sponsellor PD. (2009). Osteogenesis imperfecta: questions and answers. Curr. Opin. Pediatr. , 21, 709-16. PMID: 19907330 DOI.


Reviews

32850793

Katsimbri P. (2017). The biology of normal bone remodelling. Eur J Cancer Care (Engl) , 26, . PMID: 28786518 DOI.

Berendsen AD & Olsen BR. (2015). Bone development. Bone , 80, 14-18. PMID: 26453494 DOI.

Takigawa M. (2013). CCN2: a master regulator of the genesis of bone and cartilage. J Cell Commun Signal , 7, 191-201. PMID: 23794334 DOI.

Long F & Ornitz DM. (2013). Development of the endochondral skeleton. Cold Spring Harb Perspect Biol , 5, a008334. PMID: 23284041 DOI.

Dwek JR. (2010). The periosteum: what is it, where is it, and what mimics it in its absence?. Skeletal Radiol. , 39, 319-23. PMID: 20049593 DOI.

Yang Y. (2009). Skeletal morphogenesis during embryonic development. Crit. Rev. Eukaryot. Gene Expr. , 19, 197-218. PMID: 19883365

Mackie EJ, Ahmed YA, Tatarczuch L, Chen KS & Mirams M. (2008). Endochondral ossification: how cartilage is converted into bone in the developing skeleton. Int. J. Biochem. Cell Biol. , 40, 46-62. PMID: 17659995 DOI.

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Search Pubmed: Bone Development | developmental ossification | endochondral ossification | intramembranous ossification

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Terms

Bone Terms  
Bone Development
  • canaliculi - (singular, canaliculus) small channel in the bone matrix in which an osteocyte process lies and communicates with other osteocytes and the Haversian canal. Allow osteocytes to communicate with each other and to exchange substances by diffusion.
  • cartilage - connective tissue from mesoderm in the embryo forms most of the initial skeleton which is replaced by bone. In adult, found on the surface of bone joints.
  • Cbfa1 - Core-Binding Factor 1 (Runx2) transcription factor protein key to the differentiation of bone OMIM: Cbfa1
  • centrum - the primordium of the vertebral body formed initially by the sclerotome.
  • circumferential lamellae - compact bone layers that underlie the periosteum and endosteum (endosteal lamellae). (see concentric and interstitial lamellae)
  • clavicle - (Latin, clavicle = little key) bone which locks shoulder to body.
  • Cobb angle - clinical term for measuring axial skeleton abnormality. Measures coronal plane deformity on antero-posterior plain radiographs in the classification of scoliosis. Named after the American orthopedic surgeon John Robert Cobb (1903 - 1967).
  • concentric lamellae - compact bone layers surrounding each osteon. (see interstitial and circumferential lamellae)
  • desmal ossification - (intramembranous ossification) the process of mesenchyme directly ossifying into bone without a pre-existing cartilage template. Vascularised regions of mesenchymal cells proliferate and differentiate into pre-osteoblasts and then osteoblasts, occurs in parts of the skull and the clavicle.
  • diaphysis - anatomical term that refers to the shaft of long bones.
  • endochondrial ossification - the process of replacement of the cartilagenous framework by osteoblasts with bone.
  • endosteum - inner layer of cells lining the medullary cavity of long bones and is highly vascularised. A similar cellular region and fibrous layer lies on the outside of the bone, the periosteum.
  • epiphysis - anatomical term that refers to the expanded ends of long bones.
  • extracellular matrix - material secreted by and surrounding cells. Consists if fibers and ground substance.
  • fibroblast growth factors - (FGF) a family of at least 10 secreted proteins that bind membrane tyrosine kinase receptors. A patterning switch with many different roles in different tissues. (FGF8 = androgen-induced growth factor (AIGF)
  • fibroblast growth factor receptor - receptors comprise a family of at least 4 related but individually distinct tyrosine kinase receptors (FGFR1- 4). They have a similar protein structure, with 3 immunoglobulin-like domains in the extracellular region, a single membrane spanning segment, and a cytoplasmic tyrosine kinase domain.
  • heterotopic ossification - (HO) a disorder of extra-skeletal bone formation that occurs as a complication of trauma or in rare genetic disorders. PMID28455214
  • haematopoiesis (Greek, haima = "blood"; poiesis = "to make") the process of blood cell formation. In the adult, this occurs only in the bone marrow. In the embryo this occurs in other locations (yolk sac, liver, spleen, thymus) until bone develops.
  • Haversian canal - the central canal of an osteon (Haversian system) in compact bone, within which blood vessels and nerves travel throughout the bone.
  • Haversian system - (osteon) the historic name for the functional unit of compact bone. Consists of a central canal (Haversian canal) surrounded by lamellar bone matrix within which osteocytes reside. Named after Clopton Havers (1650-1702) an English physician and anatomist. PMID12999959
  • Howship's lacuna - (resorptive bay) the historic name for the shallow bay or cavity lying directly under an osteoclast. This is the site of bone matrix resorption. Named after John Howship (1781–1841) a British anatomist who identified this region in 1820.
  • hyoid bone - part of the axial skeleton, is a U-shape bone in the neck that anchors the tongue and is associated with swallowing.
  • interstitial lamellae - compact bone layers that fill in between each osteon, interstitial lamellae, and do appear part of any Haversian system. (see concentric and circumferential lamellae)
  • intramembranous ossification - (desmal ossification) the process of mesenchyme directly ossifying into bone without a pre-existing cartilage template. Vascularised regions of mesenchymal cells proliferate and differentiate into pre-osteoblasts and then osteoblasts, occurs in parts of the skull and the clavicle.
  • lacuna - (Latin, lacuna = “ditch, gap” diminutive form of lacus = “lake”) lacunae is the plural, cavity in bone or cartilage for cell.
  • lamellar bone - the highly organized strong bone matrix deposited in concentric sheets with a low proportion of osteocytes. Many collagen fibers parallel to each other in the same layer. Replaces woven bone.
  • medullary cavity - (bone marrow) refers to the cavity within the bone, that is lined with cells (endosteum) and filled with bone marrow. in the adult, this can also be identified as either red or yellow marrow.
  • mesenchymal progenitor cells - (MPCs) cells able to differentiate in various types of connective tissue, including cartilage, bone and adipose tissue.
  • metaphysis - anatomical term that connecting region, that lies between the diaphysis and epiphysial line.
  • Moiré topography - clinical term for measuring axial skeleton abnormality. Measures spinal deviations using Moiré topography, a biosteriometric method producing a three-dimensional image of the shape of the trunk.
  • osteoblast - The mesenchymal cells that differentiate to form the cellular component of bone and produce bone matrix. Mature osteoblasts are called osteocytes. (More? bone)
  • osteoclast - Cells that remove bone (bone resorption) by enzymatically eroding the bone matrix. These cells are monocyte-macrophage in origin and fuse to form a multinucleated osteoclast. These cells allow continuous bone remodelling and are also involved in calcium and phosphate metabolism. The erosion cavity that the cells lie iwithin and form is called Howship's lacuna. (More? bone)
  • osteocyte - The mature bone-forming cell, which form the cellular component of bone and produce bone matrix. Differentiate from osteoblasts, mesenchymal cells that differentiate to form bone. (More? bone)
  • osteon - (Haversian system) the functional unit of compact bone. Consists of a central canal (Haversian canal) surrounded by lamellar bone matrix within which osteocytes reside.
  • pedicle - (Latin, pediculus = small foot) part of the vertebral arch forming the segment between the transverse process and the vertebral body.
  • periosteum - the cellular region and fibrous layer lying on the outside of the bone.
  • primary centre of ossification - the first area where bone growth occurs between the periosteum and cartilage.
  • resorptive bay - (Howship's lacuna) the shallow bay or cavity lying directly under an osteoclast. This is the site of bone matrix resorption.
  • sclerotome - ventromedial half of each somite that forms the vertebral body and intervertebral disc.
  • Scoliometry - clinical term for measuring axial skeleton abnormality using a Pedi-Scoliometer (Pedihealth Oy, Oulu, Finland) to measure spine deviation.
  • Sharpey’s fibres - (SF) “perforating fibres” provide anchorage for the periosteum and in tooth anchorage.
  • suture - in the skull a form of articulation where the contiguous margins of the bones are united by a thin layer of fibrous tissue.
  • trabecular bone - lamellar bone not forming Haversian systems.
  • woven bone - the first deposited weaker bone matrix with many osteocytes and a matrix disorganized structure. Replaced by lamellar bone. Seen in developing, healing and bone disease.
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Cite this page: Hill, M.A. (2024, March 19) Embryology Musculoskeletal System - Bone Development. Retrieved from https://embryology.med.unsw.edu.au/embryology/index.php/Musculoskeletal_System_-_Bone_Development

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