Mouse Zygote 2 Movie

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<mediaplayer width='380' height='400' image="http://embryology.med.unsw.edu.au/embryology/images/9/9a/Mouse_zygote_division_icon.jpg">File:Mouse_zygote_division_02.mp4</mediaplayer>
Mouse zygote division 02 icon.jpg

Movie shows mitotic division of the early mouse embryo starting at the zygote stage.


The elapsed time from the start of acquisition (days:hours:minutes) is indicated.

Maximum projections of labeled histone protein (H2B-mRFP; top left) and phosphorylated H3 (H3S10ph, Fab313-488; top right) are shown with brightfield (BF; bottom left) and in merged images (bottom right; H2B and Fab313 are shown in cyan and red, respectively).

Fab313-488 is preferentially concentrated on maternal condensed chromosomes at the first division (03:19–05:19)

DNA in eukaryotes is wrapped around a histone octamer containing H2A, H2B, H3, and H4, forming a nucleosome, which is the fundamental unit of chromatin.

Links: Quicktime version | Flash version

Mouse zygote division 02 icon.jpg Mouse embryo was injected with H2B-mRFP mRNA and Fab313-488. Images of 51 focal planes (2-µm intervals) were captured at 10-min intervals using an inverted microscope (IX-71) with an UPlan-Apochromat 40× NA 1.0 oil immersion objective lens. Maximum projections of H2B-mRFP (top left) and Fab313-488 (top right) are shown with brightfield (BF; bottom left) and merged images (bottom right; H2B and Fab311 are shown in cyan and red, respectively).

The elapsed time from the start of acquisition (days:hours:minutes) is indicated. Fab313-488 is preferentially concentrated on maternal condensed chromosomes at the first division (03:19–05:19), as it recognizes histone H3S10ph next to di- or trimethylated H3K9.

Reference

<pubmed>19995936</pubmed>| JCB

Published December 14, 2009 // JCB vol. 187 no. 6 781-790 The Rockefeller University Press, doi: 10.1083/jcb.200904137 Visualizing histone modifications in living cells: spatiotemporal dynamics of H3 phosphorylation during interphase


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Cite this page: Hill, M.A. (2020, September 26) Embryology Mouse Zygote 2 Movie. Retrieved from https://embryology.med.unsw.edu.au/embryology/index.php/Mouse_Zygote_2_Movie

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© Dr Mark Hill 2020, UNSW Embryology ISBN: 978 0 7334 2609 4 - UNSW CRICOS Provider Code No. 00098G