Mouse Zygote 2 Movie: Difference between revisions

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| <html5media height="420" width="380">File:Mouse_zygote_division_02.mp4</html5media>
| <html5media height="420" width="380">File:Mouse_zygote_division_02.mp4</html5media>
[[Media:Mouse_zygote_division_02.mp4|'''Click Here''' to play on mobile device]]
| valign=top| [[File:Mouse_zygote_division_02_icon.jpg|100px|right]]
| valign=top| [[File:Mouse_zygote_division_02_icon.jpg|100px|right]]



Revision as of 10:03, 8 July 2014

Embryology - 28 Mar 2024    Facebook link Pinterest link Twitter link  Expand to Translate  
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<html5media height="420" width="380">File:Mouse_zygote_division_02.mp4</html5media>

Click Here to play on mobile device

Mouse zygote division 02 icon.jpg

Movie shows mitotic division of the early mouse embryo starting at the zygote stage.


The elapsed time from the start of acquisition (days:hours:minutes) is indicated.

Maximum projections of labeled histone protein (H2B-mRFP; top left) and phosphorylated H3 (H3S10ph, Fab313-488; top right) are shown with brightfield (BF; bottom left) and in merged images (bottom right; H2B and Fab313 are shown in cyan and red, respectively).


Fab313-488 is preferentially concentrated on maternal condensed chromosomes at the first division (03:19–05:19)

DNA in eukaryotes is wrapped around a histone octamer containing H2A, H2B, H3, and H4, forming a nucleosome, which is the fundamental unit of chromatin.


Links: MP4 version | Quicktime version | Mouse Zygote 1 Movie | Cell Division - Mitosis | Zygote | Morula | Week 1 | Movies
Mouse zygote division 02 icon.jpg Mouse embryo was injected with H2B-mRFP mRNA and Fab313-488. Images of 51 focal planes (2-µm intervals) were captured at 10-min intervals using an inverted microscope (IX-71) with an UPlan-Apochromat 40× NA 1.0 oil immersion objective lens.
  • top left- Maximum projections of H2B-mRFP
  • top right - Fab313-488
  • bottom left - brightfield (BF)
  • bottom right - merged images, H2B (cyan) and Fab311 (red).

The elapsed time from the start of acquisition (days:hours:minutes) is indicated.

Fab313-488 is preferentially concentrated on maternal condensed chromosomes at the first division (03:19–05:19), as it recognizes histone H3S10ph next to di- or trimethylated H3K9.

Reference

<pubmed>19995936</pubmed>| JCB

Published December 14, 2009 // JCB vol. 187 no. 6 781-790 The Rockefeller University Press, doi: 10.1083/jcb.200904137 Visualizing histone modifications in living cells: spatiotemporal dynamics of H3 phosphorylation during interphase


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Cite this page: Hill, M.A. (2024, March 28) Embryology Mouse Zygote 2 Movie. Retrieved from https://embryology.med.unsw.edu.au/embryology/index.php/Mouse_Zygote_2_Movie

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© Dr Mark Hill 2024, UNSW Embryology ISBN: 978 0 7334 2609 4 - UNSW CRICOS Provider Code No. 00098G