Mouse Zygote 2 Movie: Difference between revisions
(Created page with "{{Movie header}} {| | width=400px|<mediaplayer width='380' height='400' image="http://embryology.med.unsw.edu.au/embryology/images/9/9a/Mouse_zygote_division_icon.jpg">File:Mouse_zyg...") |
mNo edit summary |
||
(12 intermediate revisions by the same user not shown) | |||
Line 1: | Line 1: | ||
{{Movie header}} | {{Movie header}} | ||
{| | {| | ||
| <html5media height="420" width="380">File:Mouse_zygote_division_02.mp4</html5media> | |||
| [[File:Mouse_zygote_division_02_icon.jpg|100px|right]] | |||
[[Media:Mouse_zygote_division_02.mp4|'''Click Here''' to play on mobile device]] | |||
| valign=top| [[File:Mouse_zygote_division_02_icon.jpg|100px|right]] | |||
Movie shows mitotic division of the early mouse embryo starting at the zygote stage. | Movie shows mitotic division of the early mouse embryo starting at the zygote stage. | ||
Line 10: | Line 12: | ||
Maximum projections of labeled histone protein (H2B-mRFP; top left) and phosphorylated H3 (H3S10ph, Fab313-488; top right) are shown with brightfield (BF; bottom left) and in merged images (bottom right; H2B and Fab313 are shown in cyan and red, respectively). | Maximum projections of labeled histone protein (H2B-mRFP; top left) and phosphorylated H3 (H3S10ph, Fab313-488; top right) are shown with brightfield (BF; bottom left) and in merged images (bottom right; H2B and Fab313 are shown in cyan and red, respectively). | ||
Fab313-488 is preferentially concentrated on maternal condensed chromosomes at the first division (03:19–05:19) | Fab313-488 is preferentially concentrated on maternal condensed chromosomes at the first division (03:19–05:19) | ||
Line 15: | Line 18: | ||
DNA in eukaryotes is wrapped around a histone octamer containing H2A, H2B, H3, and H4, forming a nucleosome, which is the fundamental unit of chromatin. | DNA in eukaryotes is wrapped around a histone octamer containing H2A, H2B, H3, and H4, forming a nucleosome, which is the fundamental unit of chromatin. | ||
'''Links:''' [[ | |||
:'''Links:''' [[Media:Mouse_zygote_division_02.mp4|MP4 version]] | [[Mouse Zygote 1 Movie]] | {{mitosis}} | {{zygote}} | {{morula}} | [[Week 1]] | [[Movies]] | |||
|} | |} | ||
{| | {| | ||
| [[File:Mouse_zygote_division_02_icon.jpg]] | | [[File:Mouse_zygote_division_02_icon.jpg]] | ||
| Mouse embryo was injected with H2B-mRFP mRNA and Fab313-488. Images of 51 focal planes (2-µm intervals) were captured at 10-min intervals using an inverted microscope (IX-71) with an UPlan-Apochromat 40× NA 1.0 oil immersion objective lens. Maximum projections of H2B-mRFP | | Mouse embryo was injected with H2B-mRFP mRNA and Fab313-488. Images of 51 focal planes (2-µm intervals) were captured at 10-min intervals using an inverted microscope (IX-71) with an UPlan-Apochromat 40× NA 1.0 oil immersion objective lens. | ||
The elapsed time from the start of acquisition (days:hours:minutes) is indicated. Fab313-488 is preferentially concentrated on maternal condensed chromosomes at the first division (03:19–05:19), as it recognizes histone H3S10ph next to di- or trimethylated H3K9. | * top left- Maximum projections of H2B-mRFP | ||
* top right - Fab313-488 | |||
* bottom left - brightfield (BF) | |||
* bottom right - merged images, H2B (cyan) and Fab311 (red). | |||
The elapsed time from the start of acquisition (days:hours:minutes) is indicated. | |||
Fab313-488 is preferentially concentrated on maternal condensed chromosomes at the first division (03:19–05:19), as it recognizes histone H3S10ph next to di- or trimethylated H3K9. | |||
|} | |} | ||
===Reference=== | ===Reference=== | ||
{{#pmid:19995936}} | |||
Published December 14, 2009 // JCB vol. 187 no. 6 781-790 | Published December 14, 2009 // JCB vol. 187 no. 6 781-790 |
Latest revision as of 10:41, 24 May 2018
Embryology - 25 Apr 2024 Expand to Translate |
---|
Google Translate - select your language from the list shown below (this will open a new external page) |
العربية | català | 中文 | 中國傳統的 | français | Deutsche | עִברִית | हिंदी | bahasa Indonesia | italiano | 日本語 | 한국어 | မြန်မာ | Pilipino | Polskie | português | ਪੰਜਾਬੀ ਦੇ | Română | русский | Español | Swahili | Svensk | ไทย | Türkçe | اردو | ייִדיש | Tiếng Việt These external translations are automated and may not be accurate. (More? About Translations) |
<html5media height="420" width="380">File:Mouse_zygote_division_02.mp4</html5media> |
Movie shows mitotic division of the early mouse embryo starting at the zygote stage.
Maximum projections of labeled histone protein (H2B-mRFP; top left) and phosphorylated H3 (H3S10ph, Fab313-488; top right) are shown with brightfield (BF; bottom left) and in merged images (bottom right; H2B and Fab313 are shown in cyan and red, respectively).
DNA in eukaryotes is wrapped around a histone octamer containing H2A, H2B, H3, and H4, forming a nucleosome, which is the fundamental unit of chromatin.
|
Reference
Hayashi-Takanaka Y, Yamagata K, Nozaki N & Kimura H. (2009). Visualizing histone modifications in living cells: spatiotemporal dynamics of H3 phosphorylation during interphase. J. Cell Biol. , 187, 781-90. PMID: 19995936 DOI.
Published December 14, 2009 // JCB vol. 187 no. 6 781-790
The Rockefeller University Press, doi: 10.1083/jcb.200904137
Visualizing histone modifications in living cells: spatiotemporal dynamics of H3 phosphorylation during interphase
Copyright
Rockefeller University Press - Copyright Policy This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jcb.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at https://creativecommons.org/licenses/by-nc-sa/4.0/ ). (More? Help:Copyright Tutorial)
Glossary Links
- Glossary: A | B | C | D | E | F | G | H | I | J | K | L | M | N | O | P | Q | R | S | T | U | V | W | X | Y | Z | Numbers | Symbols | Term Link
Cite this page: Hill, M.A. (2024, April 25) Embryology Mouse Zygote 2 Movie. Retrieved from https://embryology.med.unsw.edu.au/embryology/index.php/Mouse_Zygote_2_Movie
- © Dr Mark Hill 2024, UNSW Embryology ISBN: 978 0 7334 2609 4 - UNSW CRICOS Provider Code No. 00098G