Mouse Tooth Development Movie: Difference between revisions

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Time-lapse imaging of a developing tooth germ derived from a transgenic mouse embryo (E 12.5–13.5). Co-expressing Fucci-G0/G1 probe and H2BEGFP. The tooth germ was cultured for 5 days ex vivo, and the images were taken at 30-min intervals. The lingual side is on the left.  
Time-lapse imaging of a developing tooth germ derived from a transgenic mouse embryo (E 12.5–13.5). Co-expressing Fucci-G0/G1 probe and H2BEGFP. The tooth germ was cultured for 5 days ex vivo, and the images were taken at 30-min intervals. The lingual side is on the left.  


To visualize these tooth morphogenetic dynamics sequentially, we established an ex vivo time-lapse imaging system using transgenic mouse embryos co-expressing the Fucci-G0/G1 probe (Cdt1-mKO) and enhanced green fluorescent protein-labeled human histone 2B (H2B-EGFP).  
Tooth morphogenetic dynamics viewed by an ex vivo time-lapse imaging system using transgenic mouse embryos co-expressing the Fucci-G0/G1 probe (Cdt1-mKO) and enhanced green fluorescent protein-labeled human histone 2B (H2B-EGFP).  


Note - [[:File:Mouse tooth movie 01.mp4|Original research movie]] has been modified by the addition of labelling. Tooth stage labelling is only approximate.
Note - [[:File:Mouse tooth movie 01.mp4|Original research movie]] has been modified by the addition of labelling. Tooth stage labelling is only approximate.
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==Original Research Movie==
==Original Research Movie==
<html5media height="540" width="512">File:Mouse tooth movie 02.mp4</html5media>
<html5media height="540" width="512">File:Mouse tooth movie 02.mp4</html5media>

Revision as of 15:35, 1 August 2017

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<html5media height="540" width="512">File:Mouse tooth movie 01.mp4</html5media>

Mouse Tooth Development

Time-lapse imaging of a developing tooth germ derived from a transgenic mouse embryo (E 12.5–13.5). Co-expressing Fucci-G0/G1 probe and H2BEGFP. The tooth germ was cultured for 5 days ex vivo, and the images were taken at 30-min intervals. The lingual side is on the left.

Tooth morphogenetic dynamics viewed by an ex vivo time-lapse imaging system using transgenic mouse embryos co-expressing the Fucci-G0/G1 probe (Cdt1-mKO) and enhanced green fluorescent protein-labeled human histone 2B (H2B-EGFP).

Note - Original research movie has been modified by the addition of labelling. Tooth stage labelling is only approximate.

Links: MP4 | Tooth Development | Mouse Development

Original Research Movie

<html5media height="540" width="512">File:Mouse tooth movie 02.mp4</html5media>

Links: MP4

Reference

<pubmed>27588418</pubmed>

Morita R, Kihira M, Nakatsu Y, Nomoto Y, Ogawa M, Ohashi K, et al. (2016) Coordination of Cellular Dynamics Contributes to Tooth Epithelium Deformations. PLoS ONE 11(9): e0161336. https://doi.org/10.1371/journal.pone.0161336

Copyright

© 2016 Morita et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0161336 S2 Video (MP4). https://doi.org/10.1371/journal.pone.0161336.s010


Cite this page: Hill, M.A. (2024, April 19) Embryology Mouse Tooth Development Movie. Retrieved from https://embryology.med.unsw.edu.au/embryology/index.php/Mouse_Tooth_Development_Movie

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© Dr Mark Hill 2024, UNSW Embryology ISBN: 978 0 7334 2609 4 - UNSW CRICOS Provider Code No. 00098G