Mouse Thymus Movie 1: Difference between revisions

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Adult Mouse Lymph Node - T cell motility and transendothelial migration

<html5media height="380" width="400">File:Mouse_adult_lymph_node_01.mp4</html5media>

Click Here to play on mobile device

Examples of cell death in situ during negative selection. Purified CD4+CD8+ F5 thymocytes labeled with SNARF and Hoechst were introduced into LysM-GFP thymic slices then treated with 1 nM specific peptide for 30 min. The incubation was continued for the indicated times and the slices were imaged by time-lapse two-photon microscopy. The arrowheads point to dying thymocytes.

Scale bars are 5 µm.

Reference

<pubmed>23700386</pubmed>| PLoS Biol. | PLoS Biol. Immunobiology Collection

Copyright

© 2013 Dzhagalov et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

doi:10.1371/journal.pbio.1001566.s006 (MOV)

http://journals.plos.org/plosbiology/article/asset?unique&id=info:doi/10.1371/journal.pbio.1001566.s006

Glossary Links: A | B | C | D | E | F | G | H | I | J | K | L | M | N | O | P | Q | R | S | T | U | V | W | X | Y | Z | Numbers | Symbols | Movies

Cite this page: Hill, M.A. (2024, April 24) Embryology Mouse Thymus Movie 1. Retrieved from https://embryology.med.unsw.edu.au/embryology/index.php/Mouse_Thymus_Movie_1

What Links Here?

@@ Line 7: / Line 7: @@
 [[Media:Mouse_adult_lymph_node_01.mp4|'''Click Here''' to play on mobile device]]
-| [[File:Lymph_node_cartoon_02.jpg|alt=Lymph node cartoon|500px]]
+| Examples of cell death in situ during negative selection. Purified CD4+CD8+ F5 thymocytes labeled with SNARF and Hoechst were introduced into LysM-GFP thymic slices then treated with 1 nM specific peptide for 30 min. The incubation was continued for the indicated times and the slices were imaged by time-lapse two-photon microscopy. The arrowheads point to dying thymocytes.
-|-
-| [[File:Mouse adult lymph node 01.jpg]]
+Scale bars are 5 µm.
-[[Media:Mouse_adult_lymph_node_01.mp4|Open Movie in new window]]
-| This movie shows T cell (green) motility and transendothelial (outlined by white dots) migration.
 |-
 |}
+===Reference===
+<pubmed>23700386</pubmed>| [http://journals.plos.org/plosbiology/article?id=10.1371/journal.pbio.1001566 PLoS Biol.] | [http://journals.plos.org/ploscollections/article?id=10.1371/journal.pbio.1001566 PLoS Biol. Immunobiology Collection]
-{{Movie footer}}
+====Copyright====
+© 2013 Dzhagalov et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
+doi:10.1371/journal.pbio.1001566.s006 (MOV)
-===Elimination of self-reactive T cells in the thymus: a timeline for negative selection===
+http://journals.plos.org/plosbiology/article/asset?unique&id=info:doi/10.1371/journal.pbio.1001566.s006
-PLoS Biol. 2013;11(5):e1001566. doi: 10.1371/journal.pbio.1001566. Epub 2013 May 21.
-Dzhagalov IL1, Chen KG, Herzmark P, Robey EA.
+{{Movie footer}}
-Abstract
-The elimination of autoreactive T cells occurs via thymocyte apoptosis and removal by thymic phagocytes, but the sequence of events in vivo, and the relationship between thymocyte death and phagocytic clearance, are unknown. Here we address these questions by following a synchronized cohort of thymocytes undergoing negative selection within a three-dimensional thymic tissue environment, from the initial encounter with a negative selecting ligand to thymocyte death and clearance. Encounter with cognate peptide-MHC complexes results in rapid calcium flux and migratory arrest in auto-reactive thymocytes over a broad range of peptide concentrations, followed by a lag period in which gene expression changes occurred, but there was little sign of thymocyte death. Caspase 3 activation and thymocyte loss were first detectable at 2 and 3 hours, respectively, and entry of individual thymocytes into the death program occurred asynchronously over the next 10 hours. Two-photon time-lapse imaging revealed that thymocyte death and phagocytosis occurred simultaneously, often with thymocytes engulfed prior to changes in chromatin and membrane permeability. Our data provide a timeline for negative selection and reveal close coupling between cell death and clearance in the thymus.
-Comment in
-Bringing you negative selection, live and in color. [PLoS Biol. 2013]
-PMID 23700386
-http://journals.plos.org/plosbiology/article?id=10.1371/journal.pbio.1001566
-http://journals.plos.org/ploscollections/article?id=10.1371/journal.pbio.1001566