Mouse Thymus Movie 1

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Adult Mouse Thymus - T cell Elimination

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Dying thymocytes are efficiently cleared by phagocytes.

Examples of cell death in situ during negative selection.

Purified CD4+CD8+ F5 thymocytes labeled with SNARF and Hoechst were introduced into LysM-GFP thymic slices then treated with 1 nM specific peptide for 30 min.

The incubation was continued for the indicated times and the slices were imaged by time-lapse two-photon microscopy.

The arrowheads point to dying thymocytes. Scale bars are 5 µm.

Links: Thymus Development | Immune | Movies

Mouse adult thymus 11.jpg

Mouse adult thymus 01.jpg

Reference

Dzhagalov IL, Chen KG, Herzmark P & Robey EA. (2013). Elimination of self-reactive T cells in the thymus: a timeline for negative selection. PLoS Biol. , 11, e1001566. PMID: 23700386 DOI.

Copyright

© 2013 Dzhagalov et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

doi:10.1371/journal.pbio.1001566.s006 (MOV)

http://journals.plos.org/plosbiology/article/asset?unique&id=info:doi/10.1371/journal.pbio.1001566.s006


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Cite this page: Hill, M.A. (2019, July 24) Embryology Mouse Thymus Movie 1. Retrieved from https://embryology.med.unsw.edu.au/embryology/index.php/Mouse_Thymus_Movie_1

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© Dr Mark Hill 2019, UNSW Embryology ISBN: 978 0 7334 2609 4 - UNSW CRICOS Provider Code No. 00098G