Mouse Lipid Droplets Movie

From Embryology
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Embryology - 28 Mar 2024    Facebook link Pinterest link Twitter link  Expand to Translate  
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<html5media height="580" width="465">File:Mouse lipid droplets.mp4</html5media>

Mouse Embryo Lipid Droplets

Mouse lipid droplets icon.jpg

Time-lapse of embryo development optical sections of the mouse embryo.

  • At fertilization, lipid droplets are small and uniformly distributed.
  • Implanting blastocyst lipid droplets are larger and enriched in the invading giant cells of the trophectoderm.
  • Movie starts at the compacted morula stage and development is followed till the blastocyst stage.
  • The majority of third harmonic generation (THG) signal is generated by lipid droplets, which become fewer and larger over the course of development.
  • Imaged with third harmonic generation (THG, grey) and second harmonic generation (SHG magenta).
  • Images were captured at 20 minute intervals. Scale = 20 μm.


Harmonic Generation Microscopy (HGM)

  • laser scanning microscope three-dimensional, label-free imaging of developing embryos
  • takes advantage of second and third harmonic generation (SHG and THG respectively)
  • result in the generation of photons of half and one-third the illumination wavelength respectively.


Links: MP4 version | Mouse Development

Reference

<pubmed>20525231</pubmed>| PMC3238212 | BMC Cell Biol.


Copyright

© 2010 Watanabe et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


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Cite this page: Hill, M.A. (2024, March 28) Embryology Mouse Lipid Droplets Movie. Retrieved from https://embryology.med.unsw.edu.au/embryology/index.php/Mouse_Lipid_Droplets_Movie

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© Dr Mark Hill 2024, UNSW Embryology ISBN: 978 0 7334 2609 4 - UNSW CRICOS Provider Code No. 00098G