Mouse Lipid Droplets Movie: Difference between revisions

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<mediaplayer width='462' height='550' image="http://embryology.med.unsw.edu.au/embryology/images/4/46/Mouse_trachea_01-icon.jpg">File:Mouse lipid droplets.mp4</mediaplayer>

Mouse Embryo Lipid Droplets

File:Mouse lipid droplets-icon.jpg

Time-lapse of embryo development optical sections of the mouse embryo.

The majority of third harmonic generation (THG) signal is generated by lipid droplets, which become fewer and larger over the course of development.
Imaged with third harmonic generation (THG, grey) and second harmonic generation (SHG magenta).
Imaging was started at the compacted morula stage and development followed till the blastocyst stage.
Images were captured at 20 minute intervals. Scale = 20 μm.

Harmonic Generation Microscopy (HGM)

laser scanning microscope three-dimensional, label-free imaging of developing embryos
takes advantage of second and third harmonic generation (SHG and THG respectively)
result in the generation of photons of half and one-third the illumination wavelength respectively.

Links: MP4 version | Mouse Development

Reference

<pubmed>20525231</pubmed>| PMC3238212 | BMC Cell Biol.

Copyright

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Cite this page: Hill, M.A. (2024, April 18) Embryology Mouse Lipid Droplets Movie. Retrieved from https://embryology.med.unsw.edu.au/embryology/index.php/Mouse_Lipid_Droplets_Movie

What Links Here?

@@ Line 5: / Line 5: @@
 ===Mouse Embryo Lipid Droplets===
 [[File:Mouse lipid droplets-icon.jpg|100px|right]]
-Time-lapse of embryo development: optical section. Optical sections of the mouse embryo. The majority of THG signal is generated by lipid droplets, which become fewer and larger over the course of development.
+Time-lapse of embryo development optical sections of the mouse embryo.
+* The majority of third harmonic generation (THG) signal is generated by lipid droplets, which become fewer and larger over the course of development.
+* Imaged with third harmonic generation (THG, grey) and second harmonic generation (SHG magenta).
+* Imaging was started at the compacted morula stage and development followed till the blastocyst stage.
+* Images were captured at 20 minute intervals. Scale = 20 μm.
-Imaged with third harmonic generation (THG, grey) and second harmonic generation (SHG magenta).
-Imaging was started at the compacted morula stage and development followed till the blastocyst stage.
+'''Harmonic Generation Microscopy''' (HGM)
+*  laser scanning microscope three-dimensional, label-free imaging of developing embryos
+* takes advantage of second and third harmonic generation (SHG and THG respectively)
+* result in the generation of photons of half and one-third the illumination wavelength respectively.
-Images were captured at 20 minute intervals.
+:'''Links:''' [[:File:Mouse lipid droplets.mp4|MP4 version]] | [[Mouse Development]]
-Use keyboard arrow keys to navigate frame by frame through the animation.
-Scale = 20 μm.
 |}