Difference between revisions of "Mouse Lipid Droplets Movie"

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===Mouse Embryo Lipid Droplets===
 
===Mouse Embryo Lipid Droplets===
 
[[File:Mouse lipid droplets-icon.jpg|100px|right]]
 
[[File:Mouse lipid droplets-icon.jpg|100px|right]]
Time-lapse of embryo development: optical section. Optical sections of the mouse embryo. The majority of THG signal is generated by lipid droplets, which become fewer and larger over the course of development.  
+
Time-lapse of embryo development optical sections of the mouse embryo.  
 +
* The majority of third harmonic generation (THG) signal is generated by lipid droplets, which become fewer and larger over the course of development.
 +
* Imaged with third harmonic generation (THG, grey) and second harmonic generation (SHG magenta).
 +
* Imaging was started at the compacted morula stage and development followed till the blastocyst stage.
 +
* Images were captured at 20 minute intervals. Scale = 20 μm.
  
Imaged with third harmonic generation (THG, grey) and second harmonic generation (SHG magenta).
 
  
Imaging was started at the compacted morula stage and development followed till the blastocyst stage.  
+
'''Harmonic Generation Microscopy''' (HGM)
 +
*  laser scanning microscope three-dimensional, label-free imaging of developing embryos
 +
* takes advantage of second and third harmonic generation (SHG and THG respectively)
 +
* result in the generation of photons of half and one-third the illumination wavelength respectively.
  
Images were captured at 20 minute intervals.
 
  
 
+
:'''Links:''' [[:File:Mouse lipid droplets.mp4|MP4 version]] | [[Mouse Development]]
Use keyboard arrow keys to navigate frame by frame through the animation.
 
 
 
Scale = 20 μm.
 
  
 
|}
 
|}

Revision as of 16:07, 24 April 2013

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<mediaplayer width='462' height='550' image="http://embryology.med.unsw.edu.au/embryology/images/4/46/Mouse_trachea_01-icon.jpg">File:Mouse lipid droplets.mp4</mediaplayer>

Mouse Embryo Lipid Droplets

Time-lapse of embryo development optical sections of the mouse embryo.

  • The majority of third harmonic generation (THG) signal is generated by lipid droplets, which become fewer and larger over the course of development.
  • Imaged with third harmonic generation (THG, grey) and second harmonic generation (SHG magenta).
  • Imaging was started at the compacted morula stage and development followed till the blastocyst stage.
  • Images were captured at 20 minute intervals. Scale = 20 μm.


Harmonic Generation Microscopy (HGM)

  • laser scanning microscope three-dimensional, label-free imaging of developing embryos
  • takes advantage of second and third harmonic generation (SHG and THG respectively)
  • result in the generation of photons of half and one-third the illumination wavelength respectively.


Links: MP4 version | Mouse Development

Reference

<pubmed>20525231</pubmed>| PMC3238212 | BMC Cell Biol.


Copyright

© 2010 Watanabe et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


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Cite this page: Hill, M.A. (2019, October 19) Embryology Mouse Lipid Droplets Movie. Retrieved from https://embryology.med.unsw.edu.au/embryology/index.php/Mouse_Lipid_Droplets_Movie

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© Dr Mark Hill 2019, UNSW Embryology ISBN: 978 0 7334 2609 4 - UNSW CRICOS Provider Code No. 00098G