Lymph Node Development

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Introduction

Basic structure of an adult lymph node.
Schematic of lymph node.

Lymphatic vasculature drains lymph fluid from the organ tissue space and returns it to the blood vasculature for recirculation. Lymph nodes lie on the path of lymph vessels and these structures monitor and carry out immune surveillance of this fluid for antigens and pathogens, trapping them within the lymph nodes and generating immune responses.

In early node development, vein endothelial cells form a spherical body (lymph sac) that is surrounded and then invaded by mesenchymal cells that contribute the lymph node stoma.

The reticular cells that form the stoma of the node were seen as purely structural and supportive, more recent studies have shown that the reticular network that they form is also part of the immune process.


Immune Links: immune | blood | spleen | thymus | lymphatic | lymph node | Antibody | Med Lecture - Lymphatic Structure | Med Practical | Immune Movies | vaccination | bacterial infection | Abnormalities | Category:Immune
Historic Embryology  
1909 Lymph glands | 1912 Development of the Lymphatic System | 1918 Gray's Lymphatic Images | 1916 Pig Lymphatics | 1919 Chicken Lymphatic | 1921 Spleen | 1922 Pig Stomach Lymphatics | 1932 Cat Pharyngeal Tonsil | Historic Disclaimer

Some Recent Findings

  • Development of secondary lymphoid organs in relation to lymphatic vasculature[1] "Although the initial event in lymphatic endothelial specification occurs slightly before the initiation of lymph node formation in mice, the development of lymphatic vessels and lymph nodes occurs within the same embryonic time frame. Specification of lymphatic endothelial cells starts around embryonic day 10 (E10), followed by endothelial cell budding and formation of the first lymphatic structures. Through lymphatic endothelial cell sprouting these lymph sacs give rise to the lymphatic vasculature which is complete by E15.5 in mice. It is within this time frame that lymph node formation is initiated and the first structure is secured in place. As lymphatic vessels are crucially involved in the functionality of the lymph nodes, the recent insight that both structures depend on common developmental signals for their initiation provides a molecular mechanism for their coordinated formation. Here, we will describe the common developmental signals needed to properly start the formation of lymphatic vessels and lymph nodes and their interdependence in adult life."
  • Lymph sacs are not required for the initiation of lymph node formation[2] "As proposed by Florence Sabin more than a century ago and recently validated, the mammalian lymphatic vasculature has a venous origin and is derived from primitive lymph sacs scattered along the embryonic body axis. Also as proposed by Sabin, it has been generally accepted that lymph nodes originate from those embryonic primitive lymph sacs. However, we now demonstrate that the initiation of lymph node development does not require lymph sacs."
More recent papers
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Search term: Lymph Node Embryology

<pubmed limit=5>Lymph Node Embryology</pubmed>

Early Development

  1. lymph sacs (primitive lymph sacs) form from endothelial cells.
  2. bud from the veins during early development
  3. then form buds that branch and form the lymphatic network.
  4. lymphoid tissue inducer cells - (LTi) first hematopoietic cells to enter and induce lymphoid tissue development.

Molecular

Prox1

  • expressed by lymphatic endothelial cells.

Reticular Cells

Mesoderm derived fibroblastic reticular cells (FRCs, RCs) form the stromal supporting network of cells and extracellular matrix in secondary lymphoid organs (lymph nodes, spleen and thymus). These specialized myofibroblasts form the structural "sponge" within lymphoid tissue, through with T cells, B cells, dendritic cells (DCs), plasma cells and macrophages interact and move.[3]

  • synthesise reticular fibres (type III collagen) that form the main extracellular matrix component
  • differentiate as distinct subpopulations distributed in the cortical and medullar regions
    • T cell zone fibroblastic reticular cells (TRCs)
  • bind dendritic cells that initiate immunity by presenting antigens to T lymphocytes also initiate remodelling of lymph node RCs
  • may regulate cellular traffic and activation of specific lymphocyte populations

Model of Reticular Network Formation[4]

Reticular network formation model.jpg

Accumulation of lymphocytes induces alteration of mesenchymal progenitors. Lymphocytes and differentiated FRCs gradually degrade preexisting matrix, and FRCs weave RF meshwork from the newly produced components. Mature RN forms and antigen presentation occur on the stromal reticulum.


Lymph node cells 01.jpg

Lymph Node and Reticular Cell Classification[5]

Molecular

  • express myofibroblast markers - desmin, vimentin, CD90, CD73, CD103, α-smooth muscle actin (αSMA) and ERTR7 antigen.
  • express - podoplanin (PDPN), platelet-derived growth factor receptor-α (PDGFRA) and genes from antigen presentation and cytokine response pathways.
  • lack of expression of CD45 and CD31.


Links: Connective Tissue Development

Dendritic Cells

Dendritic cells (DCs, antigen-presenting cells, APCs) present antigens and induce a primary immune response in resting naïve T lymphocytes. These cells originate from the same common progenitor as monocytes.[6] Recently it has been shown that recirculation of antigen-bearing dendritic cells in remote lymphoid organs can prime T cells in other locations.[7]

In 2011 Ralph M. Steinman received half the Nobel Prize half of the award to to Ralph M. Steinman for his discovery of the dendritic cell and its role in adaptive immunity.

Adult Lymph Node

  • Encapsulated organ (1 mm - 2 cm)
  • In lymph vessel pathways “filter”
  • Afferent- towards node
  • Efferent- away from node
  • Location throughout the entire body - Concentrated in axilla, groin, mesenteries
  • Antigen transformed lymphocytes from the blood
Lymph node cartoon 02.jpg

Legend

  • B - B-cell zones
  • DC - dendritic cells
  • FRC - fibroblastic reticular cells
  • HEV - high endothelial venues

Lymph Node Cartoon Gallery

Links: Immunobiology - Figure 1.8. Organization of a lymph node | MBoC Figure 24-16. A simplified drawing of a human lymph node
Lymph node structure 02.jpg Schematic representation of the organization of a lymph node.[8]
  • Afferent lymphatics enter lymph nodes and deliver lymph to the subcapsular sinus (SCS), which forms a channel around the periphery of the lymph node.
  • Lymphatic sinuses run from the SCS through the cortex to the medulla, and exit the lymph node via efferent lymphatic vessels on the opposite, hilar, side of the organ.
  • B cell follicles containing follicular dendritic cell (FDC) networks are arranged in the lymph node cortex and are separated from the SCS by a layer of marginal reticular cells (MRC).
  • T cells zones in the paracortex, which contain many fibroblastic reticular cells (FRC), are separated by the cortical ridge, an area rich in T cells, dendritic cells (DCs), blood vessels, and FRC.
  • Blood vessels enter and exit the lymph node on the hilar side, and snake through the lymph node like the branches of a tree.
  • Specialized high endothelial venules (HEVs) in the paracortex and cortical ridge allow entry of leukocytes from the blood.

Lymph node structure 01.jpg

Mesenteric Lymph Node

Gastrointestinal tract intestine immune cartoon 01.jpg

Gastrointestinal tract intestine immune overview showing mesenteric lymph nodes.[9]

Histology

Lymph Node Histology: Subcapsular Sinus | Follicle | Germinal Centre | Medullary Cords and Sinuses | High Endothelial Venules | Macrophages | Node cartoons

Adult Lymph Node Structure

  • Capsule - dense connective tissue
  • Trabeculae - dense connective tissue
  • Reticular Tissue - Reticular cells and fibers, supporting meshwork
  • Macrophages - process antigen, difficult to distinguish from the reticular cells.

Lymph node histology 02.jpg Lymph node histology 03.jpg

Lymph

  • enters the node through afferent vessels
  • filters through the sinuses
  • leaves through efferent vessels

Subcapsular sinus = marginal sinus

Lymph node histology 01.jpg

Continuation of trabecular sinus

Lymph node histology 04.jpg

Adult Lymphocytes

Lymphocyte Electron Micrographs

Cell Trafficking into and out of Lymph Nodes

Lymph node cartoon 02.jpg

Lymphocyte Traffic in and out of the Lymph Node[10]

The following data is from a recent article[11] and review[12] of live adult mouse lymphocytes (T and B cells) imaged within a lymph node.

Both lymphocyte types:

  • Spend 8 to 24 h in the lymph node interstitium.
  • Transit across a lymphatic endothelium to exit.
  • Enter a network of medullary sinuses.
  • Drain from sinuses into efferent lymphatic vessels.

Lymphocyte Migration Speeds

T cells - 10–12 μm/min in the follicle diffuse cortex, peak velocities up to 30 μm/min. (move more slowly in the medullary region near the hilus of the lymph node than in the paracortex)

B cells - 6 μm/min in the follicle diffuse cortex, peak velocities up to 20 μm/min.

Both cortical T cells and follicular B cells move in random directions following "guide cells".

Lymphocyte Guide Cells

FDC - Follicular Dendritic Cells, may guide B cells in the follicle.

FRC - Fibroblastic Reticular Cells, may guide T cells in the follicle.

Lymphocyte Movies

Adult Mouse Lymphocyte Motility
Mouse adult lymph node 01.jpg
 ‎‎Lymph Node 1
Page | Play
Mouse adult lymph node 02.jpg
 ‎‎Lymph Node 2
Page | Play
Mouse adult lymph node 03.jpg
 ‎‎Lymph Node 3
Page | Play
Mouse adult lymph node 04.jpg
 ‎‎Lymph Node 4
Page | Play
Transendothelial
migration
T cell zone Medullary sinus Sinus endothelial
barrier
Mouse adult lymph node 05.jpg
 ‎‎Lymph Node 5
Page | Play
Mouse adult lymph node 06.jpg
 ‎‎Lymph Node 6
Page | Play
Mouse adult lymph node 07.jpg
 ‎‎Lymph Node 7
Page | Play
Mouse adult lymph node 08.jpg
 ‎‎Lymph Node 8
Page | Play
Bi-directional traffic Cross the sinus
endothelial barrier
T and B cell motility T and B cell coupling
Mouse Immune Movies: Transendothelial migration | T cell zone | Medullary sinus | Sinus endothelial barrier | Bi-directional traffic | cross the sinus endothelial barrier | T and B cell motility | T and B cell coupling | T cell Elimination | Immune System Development | Mouse Development

References

  1. <pubmed>24276888</pubmed>
  2. <pubmed>19060331</pubmed>
  3. <pubmed>25998961</pubmed>
  4. <pubmed>15381731</pubmed>| J Exp Med.
  5. <pubmed>24829927</pubmed>| J Immunol Res.
  6. <pubmed>20193011</pubmed>
  7. <pubmed>23801305</pubmed>
  8. <pubmed>19644499</pubmed>| PMC2785037 | Nat Rev Immunol.
  9. <pubmed>16533891</pubmed>| PMC2118258 | J Exp Med.
  10. <pubmed>15122201</pubmed>| Nat Rev Immunol.
  11. <pubmed>16273098</pubmed>
  12. <pubmed>18173372</pubmed>


Textbook

Immunobiology 5th edition The Immune System in Health and Disease Charles A Janeway, Jr, Paul Travers, Mark Walport, and Mark J Shlomchik.

Part I. An Introduction to Immunobiology and Innate Immunity

Part III. The Development of Mature Lymphocyte Receptor Repertoires

Reviews

<pubmed></pubmed>

Articles

<pubmed>165702</pubmed> <pubmed>1167215</pubmed>

Search Pubmed

Search Pubmed: Lymph Node Development | Lymphocyte Development

External Links

External Links Notice - The dynamic nature of the internet may mean that some of these listed links may no longer function. If the link no longer works search the web with the link text or name. Links to any external commercial sites are provided for information purposes only and should never be considered an endorsement. UNSW Embryology is provided as an educational resource with no clinical information or commercial affiliation.

Terms

Immune Development

  • adenoid - (Greek " +-oeides = in form of) in the form of a gland, glandular; the pharyngeal tonsil.
  • afferent lymph - vessel carrying lymph towards a node containing antigen-presenting cells, antigen, effector and memory T cells, and regulatory T cells.
  • acquired immune deficiency syndrome - (AIDS) note this is now better described as "advanced HIV disease", decrease in the number of CD4 T cells. (More? Immunobiology - AIDS)
  • anastomose - joining of two tubes or structures together.
  • Antibody mediated immunity - the immune function of plasma cells (active B lymphocytes) secreting antibody which binds antigen.
  • antibodies - mammals have five classes (IgA, IgD, IgE, IgG, and IgM)
  • antigen - any substance that is recognised by the immune system and stimulates antibody production.
  • appendix - is a gut-associated lymphoid tissue (GALT) located at the beginning of the colon. The anatomy is as a finger-like structure that arises from the cecum. The length (2.5-13 cm) is longer in both infants and children and also has more abundant lymphatic tissue in early life. The wall structure is similar to the small intestine (though with no villi), nor plicae circularis. Lymph nodules surround the lumen of the gastrointestinal tract and extend from the mucosa into the submucosa.
  • B lymphocyte - (B cell, B-cell)
  • BALT - (Bronchus Associated Lymphoid Tissue) immune tissue associated with the respiratory tract.
  • band cell - (band neutrophil or stab cell) immature neutrophil seen in bone marrow smear, a cell undergoing granulopoiesis, derived from a metamyelocyte, and leading to a mature granulocyte. Also occasionally seen in circulating blood.
  • bone marrow sinusoid - endothelial cells and no supporting cells vascular space supplied by arteriole and capillary vessels, interconnected by inter-sinusoidal capillaries, spanning throughout the bone marrow. Radially distributed around the draining central sinus (about 100 µm in diameter). Bone marrow sinusoids are unique and are not comparable with regular veins.
  • cecum - (caecum, Latin, caecus = "blind") within the gastrointestinal tract a pouch that connects the ileum with the ascending colon of the large intestine.
  • cell - has a specific cell biology definition, but is often used instead of "lymphocyte" when describing B and T cells.
  • "clockface" - a term used to describe the appearance of plasma cell nuclei due to the clumping of the chromatin at the nucleus periphery. More clearly seen in tissue plasma cells that the bone marrow smear, where they are sometimes confused with the basophilic erythroblasts. Image - plasma cell
  • CD - (cluster of differentiation) identifies immunological surface markers on cells. Positive (+) generally means that the substance is expressed/identified, while negative (-) means that it is missing/not identified.
  • CD4+ - (T helper cells) refers to T lymphocytes that express CD4 (cluster of differentiation 4, a glycoprotein of the immunoglobulin superfamily) on their surface, associated with helper/inducer function. These cells can be infected by human immunodeficiency virus (HIV).
  • CD4/CD8 ratio - clinical measurement of different immune cell types (ratios between 1.5 to 2.5 are considered normal). Viral infections such as HIV, cytomegalovirus, Epstein-Barr virus, and influenza virus, associated with an inversion of the ratio.
  • CD8+ - (cytotoxic T cells) refers to T lymphocytes that express CD8 (glycoprotein of the immunoglobulin superfamily) on their surface, associated with cytotoxic/suppressor activity.
  • "clockface" - a term used to describe the appearance of plasma cell nuclei due to the clumping of the chromatin at the nucleus periphery. More clearly seen in tissue plasma cells that the bone marrow smear, where they are sometimes confused with the basophilic erythroblasts.
  • cords of Billroth - spleen cellular columns located in red pulp. surrounded by splenic sinusoids. Cords contain reticular cells, macrophages, lymphocytes, plasma cells and erythrocytes.
  • cortex - outer layer, used in association with medulla (innner layer or core) a general description that can be applied to describing an organ with a layered structure.
  • cortical Thymic Epithelial Cell - (cTEC, types I - IV) support and antigen presenting cells located in the cortex regions of the thymus required for positive and negative selection of maturing T cells. See also medullary epithelial cell.
  • crypt - (tonsil crypt) tonsil squamous epithelium infold, with intraepithelial passages containing non-epithelial cells. Functions include: intimate contact between immune response effector cells, facilitate transport of antigens, synthesise secretory components, and contain a pool of immunoglobulins. PMID 7559106
  • dendritic cell - (DC, antigen-presenting cell, APC) cells that present antigens and induce a primary immune response in resting naïve T lymphocytes. Originate from the same common progenitor as monocytes (PMID 20193011). In 2011 Ralph M. Steinman received half the Nobel Prize half of the award to to Ralph M. Steinman for his discovery of the dendritic cell and its role in adaptive immunity.
  • Effector cells - the immune functioning (active) B and T lymphocytes.
  • Efferent lymph - vessel carrying lymph away from a node.
  • fibroblastic reticular cell - (FRC) specialized myofibroblasts that form the structural mesenchymal network "sponge" within lymphoid tissue that regulate immune cell migration, activation, and survival. Immune T cells, B cells, dendritic cells (DCs), plasma cells and macrophages move and interact.
  • follicular dendritic cell - (FDC) in B cell follicles of secondary lymphoid organs, cells interspersed within the stromal cell network function: Primary - help B cells to cluster. Secondary - in GC long-term retention of intact antigen and support B cell survival.
  • GALT - Gut Associated Lymphatic Tissue consisting of Peyer’s patches, isolated lymphoid follicles and mesenteric lymph nodes.
  • germinal centre - (GC) centre of B cell follicles of secondary lymphoid organs, where antigen-activated B-cell clones expand and undergo immunoglobulin gene hypermutation and selection.
  • haemopoiesis (hemopoiesis) formation of blood cells.
  • high endothelial venule - (HEV) the specialised post-capillary venous region that enables blood lymphocytes and pre-dendritic cells to enter a lymph node. The endothelial cells express ligands that bind lymphocytes, aiding their adhesion and subsequent transmigration into the lymph node. With inflammation, monocytes and NK cells can also enter here.
  • humoral immune response - production of antibody by plasma cells derived from B lymphocytes (B cells).
  • IEL - Intraepithelial Lymphocyte are T lymphocytes located in the gastrointestinal tract epithelium. Natural IELs (previously ‘type b’ IELs) acquire activated phenotype during development in the thymus in the presence of self antigens. Induced IELs (previously ‘type a’ IELs) progeny of conventional T cells activated post-thymically in response to peripheral antigens.
  • IgA - the main class of antibody released at mucosal surfaces and in secretions (saliva, tears, milk, and respiratory and intestinal secretions) and the most abundantly produced antibody (70%). PMID 22566964
  • IgD - the immunoglobulin B cell starts to produce as a cell-surface molecule after leaving the bone marrow.
  • IgE - bind Fc receptors (surface of mast cells in tissues and basophils in the blood) release of potent pro inflammatory molecules mediators of allergic reactions.
  • IgG - the major class of immunoglobulin in the blood.
  • IgM - the first class of antibody made by a developing B cell, which may switch to making other classes of antibody.
  • immunoglobulin - (antibody, Ab) protein produced by plasma cells.
  • immunosenescence - in ageing and disease, refers to a weaker immune responses producing a progressive deterioration and increased susceptibility to infectious diseases, neoplasia, and autoimmune diseases.
  • innate lymphoid cells - (ILCs) subset of lymphocytes that lack antigen-specific receptors, are located in peripheral tissues and abundant at barrier surfaces, decrease in number with age. PMID 29924974
  • Kupffer cells - stellate macrophage cells located in the liver sinusoids, named after Karl Wilhelm von Kupffer (1829 - 1902) a German anatomist who originally identified these cells. (More? Liver Development)
  • lacteal - term used to describe the lymphatic vessels of the small intestine.
  • lamina propria - a layer of loose connective tissue found underneath an epithelium, together with the epithelium described as mucosa.
  • Langerhans cell - (LC, dendritic cell) Antigen-presenting immune cell found mainly in the basal/suprabasal layers of adult skin and mucosa. Cells lie in the basal/suprabasal layers of stratified epidermal and mucosal tissues. First in the innate antiviral immune defines and can migrate to lymph nodes and induce a T cell–mediated adaptive immune response. (More? Integumentary | Immune System Development)
  • Leukocyte - (Greek, lukos = clear, white) white blood cell.
  • lingual - related to the tongue, as in lingual tonsil, forms part of Waldeyer’s ring.
  • lymph node - connective tissue encapsulated lymphoid organ (1mm - 2cm in size), positioned in the pathway of lymph vessels. (More? Lymph Node Development)
  • lymphangion - the functional unit of a lymph vessel that lies between two semilunar (half moon-shaped) valves.
  • lymphangiogenesis - formation of new lymph vessels from pre-existing lymphatic structures. During embryogenesis and in adult tissues as reaction to inflammation or injury.
  • M cell - (microfold cell) found in the follicle-associated epithelium of the Peyer's patch. Function to transport gut lumen organisms and particles to immune cells across the epithelial barrier.
  • MALT - Mucosa Associated Lymphoid Tissue.
  • medulla - inner layer or core, used in association with cortex (outer layer) a general description that can be applied to describing an organ with a layered structure.
  • medullary Thymic Epithelial Cell - (mTEC, types I-VII) support and antigen presenting cells located in the medullary regions of the thymus, required for central tolerance (negative selection) of maturing T cells (PMID 11375064). See also cortical thymic epithelial cell.
  • Memory Cell - effector T cell (lymphocyte)
  • Mononuclear Phagocytic System - (MPS, Lymphoreticular System, Reticuloendothelial System, RES) Consists of circulating monocytes in the peripheral blood and non-circulating (fixed) tissue macrophages (MΦ) located in tissues and organs.
  • NAVL - (naval) mnemonic to remember the neurovascular bundle components Nerve Artery Vein Lymph found travelling together within organs and tissues.
  • negative selection - T cells bearing autoreactive T cell antigen receptors (TCRs) are eliminated during their development in the thymus, protects against autoimmunity.
  • normoblast - seen in bone marrow smear, a developing erythroblast (red blood cell) that still retains a nucleus.
  • nude mice - (nu/nu) mice which are congenitally hairless and athymic, therefore they do not reject tissue and tumor grafts.
  • PALS - acronym for PeriArterial Lymphoid Sheath in the spleen white pulp.
  • parenchyma - (Greek = enkeim "to pour in") cells forming the functional cells of an organ or tissue. These cells carry out the function of the organ at a cellular level, and are not the structural cells, connective tissue, extracellular matrix (stromal).
  • periarterial lymphoid sheath - (PALS) in the spleen the white pulp that surrounds the central arteries. (T-lymphocytes,macrophages and plasma cells)
  • pharyngeal pouch III - origin of endodermal component of the thymus (also formed from neural crest). Pharyngeal arches
  • Plasma Cell - active B cell (lymphocyte) which is secreting antibody. Located in either bone marrow or peripheral lymphoid tissues, these cells have and increased cytoplasmic volume (due to increase rough endoplasmic reticulum) in comparison to the inactive (non-secreting) lymphocyte.
  • primary follicle - follicle that does not contain germinal centre, secondary follicles do germinal centre.
  • red pulp - spleen region, organized as cell cords (splenic cords, cords of Billroth) and vascular sinuses.
  • regulatory T cells - (Tregs) maintain self tolerance and suppress pathological immune responses by control of immune response to non-self antigens.
  • reticular fibres - reticular cells secrete this extracellular matrix protein, composed of type III collagen.
  • right lymphatic duct - drains most of the right upper quadrant. See also thoracic duct.
  • secondary follicle - contain germinal centre, primary follicle does not contain germinal centre.
  • sentinel lymph node - the hypothetical first lymph node or group of nodes reached by metastasizing cancer cells from a primary tumour.
  • sinus - a larger vessel or space usually curved that may contain air, blood, or lymph. e.g. splenic medullary sinus, lymph node medullary sinus, sub-capsular sinus, trabecular sinus.
  • sinusoid - a tiny vessel with a tortuous path and many connections to similar vessels. e.g. hepatic and bone marrow sinusoids.
  • splenic capillary sheaths - in spleen around capillary endothelium and consist of three main cell types: CD271+ stromal capillary sheath cells, CD68+CD163− macrophages and recirculating B-lymphocytes. Sheaths may; 1. allow interaction among sheath macrophages and B-lymphocytes, 2. attract recirculating B-lymphocytes from the open circulation of the red pulp to start migration into white pulp follicles. 30356180
  • splenic sinusoids - enlarged splenic spaces located in red pulp and surrounding cords of Billroth.
  • sphingosine-1-phosphate - (S1P) sphingolipid secreted into the extracellular space establishing a gradient acting through G protein-coupled receptors to attract lymphocytes out of lymphoid organs (lymph node, thymus, spleen) into the circulation.
  • stroma - (Greek = "a cover, table-cloth, bedding") tissue forming the framework/support of an organ or tissue. That is the structural cells which form connective tissue and secrete extracellular matrix, rather than the functional cells (parenchymal). All organs can therefore be functionally divided into these 2 components, stromal/parenchymal.
  • Subcapsular sinus (=marginal sinus) space lying under the connective tissue capsule which receives lymph from afferent lymphatic vessels.
  • T cell - (T-cell, T lymphocyte) named after thymus, where they develop, the active cell is responsible for cell-mediated immunity (killer T cells and helper T cells). Cells express T-cell receptor on surface and directly kill virally or bacterially infected cells. These cells can themselves be infected by HIV. (More? Electron micrographs of nonactivate and activated lymphocytes)
  • TEC - (Thymic Epithelial Cell) thymus support and antigen presenting cells further divided anatomically and functionally into medullary TEC (mTEC, types I-VII, for central tolerance) and cortical epithelial cell (cTEC, types I-IV, positive and negative selection) populations (see PMID 28800929 PMID 30308217).
  • T cell activation - (T lymphocyte activation)The activation process begins with T-cells searching for and encountering antigen-bearing dendritic cells within lymph nodes.
  • thoracic duct - (TD) largest and main lymphatic vessel, drains the lower body including the extremities and abdomen. Intra-thoracic tributaries include: intercostal, mediastinal, and bronchomediastinal trunks.


  • Thymic corpuscle - (Hassall's corpuscle) a mass of concentric epithelioreticular cells found in the thymus. The number present and size tend to increase with thymus age. (see classical description of Hammar, J. A. 1903 Zur Histogenese und Involution der Thymusdriise. Anat. Anz., 27: 1909 Fiinfzig Jahre Thymusforschung. Ergebn. Anat. Entwickl-gesch. 19: 1-274.)
  • thymic epitheliocytes - reticular cells located in the thymus cortex that ensheathe the cortical capillaries, creating and maintain the microenvironment necessary for the development of T-lymphocytes in the cortex.
  • T helper cells - (helper T-cells) (Th cells, CD4+) refers to T lymphocytes that when mature express CD4 (glycoprotein of the immunoglobulin superfamily) on their surface.
  • T lymphocyte - (T cell, T-cell) regulate cell-mediated immunity.
  • thymus - an immune/endocrine (thymic hormone, thymosins) organ involved in the maturation (differentiation) of T lymphocytes (T-cells).
  • tonsils - lymph nodules embedded in the mucus membranes located at the back of the mouth and top of the throat. The overlying epithelium helps identify the location.
  • tonsillitis - a common bacterial infection of the palatine tonsils, occurring mostly in children and young adults and can also become recurrent tonsillitis.
  • vermiform appendix - see appendix, anatomical region containing gut-associated lymphoid tissue located within the gastrointestinal tract at the beginning of the colon. The anatomy is as a finger-like structure that arises from the cecum. The length (2.5-13 cm) is longer in both infants and children and also has more abundant lymphatic tissue in early life. The wall structure is similar to the small intestine (though with no villi), nor plicae circularis. Lymph nodules surround the lumen of the gastrointestinal tract and extend from the mucosa into the submucosa.
  • VDJ recombination - (variable, diversity and joining gene segments) genetic recombination event that occurs in immune cell maturation in primary lymphoid organs, B cells ((bone marrow) and T cells (thymus).
  • Waldeyer’s ring - ring of lymphoid tissue in the pharyngeal wall: palatine tonsils, nasopharyngeal tonsil (adenoid) and lingual tonsil. First described in 1884 by von Waldeyer-Hartz.
  • white pulp - (Malpighian follicles, Malpighian bodies of the spleen, white nodules, splenic lymphoid nodules) spleen lymphoid region, organized as lymphoid sheaths with both T-cell and B-cell compartments, around the branching arterial vessels (resembles lymph node structure).


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Cite this page: Hill, M.A. (2024, March 28) Embryology Lymph Node Development. Retrieved from https://embryology.med.unsw.edu.au/embryology/index.php/Lymph_Node_Development

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© Dr Mark Hill 2024, UNSW Embryology ISBN: 978 0 7334 2609 4 - UNSW CRICOS Provider Code No. 00098G