Lecture - Stem Cells: Difference between revisions
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2012 - This lecture will be presented by an expert guest lecturer '''A/Prof Kuldip Sidhu''', Director, Stem Cell Lab, Chair, Stem Cell Biology. | 2012 - This lecture will be presented by an expert guest lecturer '''A/Prof Kuldip Sidhu''', Director, Stem Cell Lab, Chair, Stem Cell Biology. | ||
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! About A/Prof Kuldip Sidhu | |||
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Kuldip S Sidhu | |||
A/Professor Kuldip Sidhu, PhD, BSC (Medical) is the chair of stem cell biology and is the director of the Stem Cell Laboratory (SCL), Faculty of Medicine, the University of New South Wales, Australia. His post doc training is from St Louis MO USA (1979-80) in assisted reproductive technology and he also worked with Prof James Thompson, Wisconsin USA (2000) who produced the first human embryonic stem cells in 1998. | |||
His research focus is on neural stem cells derived from both the embryonic and non-embryonic sources for eveloping future cell therapies for various neurodegenerative diseases, like Alzheimer’s, Parkinson’s and other neuronal diseases. | |||
He has established a state-of-the-art facility to study the cell and developmental biology of stem cells. SCL has expertise to culture, propagate, differentiate, engineer and transplant in animal models the neural stem cells from various sources like human embryonic stem cells, skin-derived neuroprogenitors and human mesenchymal stem cells from bone marrow. In addition, he has expertise in the derivation of new human embryonic stem cell lines including their clonal propagation. His lab was first to produce two hESC lines, Endeavour (E) 1&2 from Australia and E2 is listed on NIH registry USA for distribution. SCL has seriously embarked on iPS Technology and produced over 100 iPSC clones from Alzheimer’s patients for studying the disease processes and drug discovery. | |||
A/Prof Sidhu has produced two books – the latest one (2012) on stem cells, thirteen book chapters, nine review chapters, two international patents, four proprietary items and one hundred and seventy original research papers including abstracts in journal of repute including one in Nature Biotechnology (2011) and all dealing with mammalian cell and developmental biology including stem cells. He has served on the International Society of Stem Cell Research Sub Committee and the NHMRC Cell Therapy Advisory Committee; he is a member of the editorial board of International Stem Cell Journals, the open stem cell journal and recent patent on regenerative medicine. He is on the expert panel on iPSC research for the European Union. He is president of the local chapter and member of the board of Society for Brain Mapping and Therapeutics, USA. He was the chair of the program committee of the 4th annual meeting of the Australasian society of stem cell research held in Sydney 2011. He has eight national and five international active research collaborations and including three with industry. He has widely travelled around the world and presented invited lectures, chaired sessions in scientific meetings, conferences. | |||
He is recognised by many awards e.g. Medallist for outstanding research from Indian National Science Academy 1981, Best book prize, 1996, Medal for best presentation in an international conference on frontiers of reproductive biology, 1989, Best invention prize, Australia, 2007, Finalist of Eureka prize 2009, Advanced Innovation Award (Finalist), UNSW 2012. He has produced 9 PhD, 2 MSC, 4 HONS students and some of them are also recognised with Dean’s list and McConaghy Prize. | |||
His passion in science is as good as in Tennis. | |||
==Textbooks== | ==Textbooks== |
Revision as of 09:02, 9 October 2012
Introduction
Embryology is all about stem cells, a single cell (zygote) divides and differentiates to form all the tissues throughout the body. Furthermore within some tissues, stem cells remain to continuously replace cells that are lost through the life of that tissue. In recent years Scientific and general interest in this topic has increased due to the many issues that surround this specialised cell type. This lecture will introduce the various types/sources of stem cells as well as their practical and therapeutic potentials. A brief discussion of the pros and cons of different types of stem cells currently investigated in the field of medical research will be discussed.
2012 - This lecture will be presented by an expert guest lecturer A/Prof Kuldip Sidhu, Director, Stem Cell Lab, Chair, Stem Cell Biology.
About A/Prof Kuldip Sidhu | |||||||||||||||||||||||||||||||||||||||||||||
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Kuldip S Sidhu A/Professor Kuldip Sidhu, PhD, BSC (Medical) is the chair of stem cell biology and is the director of the Stem Cell Laboratory (SCL), Faculty of Medicine, the University of New South Wales, Australia. His post doc training is from St Louis MO USA (1979-80) in assisted reproductive technology and he also worked with Prof James Thompson, Wisconsin USA (2000) who produced the first human embryonic stem cells in 1998. His research focus is on neural stem cells derived from both the embryonic and non-embryonic sources for eveloping future cell therapies for various neurodegenerative diseases, like Alzheimer’s, Parkinson’s and other neuronal diseases. He has established a state-of-the-art facility to study the cell and developmental biology of stem cells. SCL has expertise to culture, propagate, differentiate, engineer and transplant in animal models the neural stem cells from various sources like human embryonic stem cells, skin-derived neuroprogenitors and human mesenchymal stem cells from bone marrow. In addition, he has expertise in the derivation of new human embryonic stem cell lines including their clonal propagation. His lab was first to produce two hESC lines, Endeavour (E) 1&2 from Australia and E2 is listed on NIH registry USA for distribution. SCL has seriously embarked on iPS Technology and produced over 100 iPSC clones from Alzheimer’s patients for studying the disease processes and drug discovery. A/Prof Sidhu has produced two books – the latest one (2012) on stem cells, thirteen book chapters, nine review chapters, two international patents, four proprietary items and one hundred and seventy original research papers including abstracts in journal of repute including one in Nature Biotechnology (2011) and all dealing with mammalian cell and developmental biology including stem cells. He has served on the International Society of Stem Cell Research Sub Committee and the NHMRC Cell Therapy Advisory Committee; he is a member of the editorial board of International Stem Cell Journals, the open stem cell journal and recent patent on regenerative medicine. He is on the expert panel on iPSC research for the European Union. He is president of the local chapter and member of the board of Society for Brain Mapping and Therapeutics, USA. He was the chair of the program committee of the 4th annual meeting of the Australasian society of stem cell research held in Sydney 2011. He has eight national and five international active research collaborations and including three with industry. He has widely travelled around the world and presented invited lectures, chaired sessions in scientific meetings, conferences. He is recognised by many awards e.g. Medallist for outstanding research from Indian National Science Academy 1981, Best book prize, 1996, Medal for best presentation in an international conference on frontiers of reproductive biology, 1989, Best invention prize, Australia, 2007, Finalist of Eureka prize 2009, Advanced Innovation Award (Finalist), UNSW 2012. He has produced 9 PhD, 2 MSC, 4 HONS students and some of them are also recognised with Dean’s list and McConaghy Prize. His passion in science is as good as in Tennis. Textbooks
Objectives
Why Stem Cells
Medline Search stem cell 2002 - 110,920 | 2004 - 128,485 | 2005 - 140,966 | 2006 - 154,176 | 2011 - 190,069 | 2012 - 210,393 Research that led to Stem Cells
Stem Cell TypesTissue Stem Cells
Blood Cells
Embryonic Stem CellsNIH - What are embryonic stem cells?
Blastocyst
Stem Cell Definition
Chimeric Mouse
Embryoid Bodies
Historic References
Cord Blood Stem Cells
Adult Stem Cells
Epithelium Stem Differentiation
Amplifying Cells
Stem Cell Production - Stem Cell Daughter Fates
Induced Pluripotent Cells
Yamanaka FactorsA set of 4 transcription factors when introduced into cells induces stem cell formation.[2] These four transcription factors can be expressed from doxycycline (dox)-inducible lentiviral vectors. The only culture difference in iPS cells and human embryonic stem cell culture is that iPS cell culture require 100ng/ml of bFGF in the culture media. Outline of the MEF reprogramming protocol 1 Outline of the MEF reprogramming protocol 2 | stained with anti-Rex1, Sox2 and SSEA1 antibodies
Stem Cell MarkersIn order to carry out research on stem cells, it is important to be able to identify them. A number of different research groups in the late 90's generated several antibodies which specifically identified undifferentiated, differentiating or differentiated stem cells from a number of different sources and species. Note that the nomenclature in some cases is based upon the antibody used to identify the cell surface marker. Morula cell lines express ES markers[4]
Expression of Zfp42/Rex1 Gene - used as a marker of undifferentiated stem cells[5]
Embryonic vs Adult Stem CellsEmbryonic Stem Cell Advantages
Embryonic Stem Cell Disadvantages
Adult Stem Cell Advantages
Adult Stem Cell Disadvantages
Current stem cell researchHow to:
ReferencesTextbooks
Search
Images
References
JournalsCo-ordinator Note
Course Content 2011 |
Embryology Introduction | Fertilization | Cell Division/Fertilization | Week 1 and 2 Development | Week 3 Development | Week 1 to 3 | Mesoderm Development | Ectoderm, Early Neural, Neural Crest | Trilaminar Embryo to Early Embryo | Early Vascular Development | Placenta | Vascular and Placenta | Endoderm, Early Gastrointestinal | Respiratory Development | Endoderm and Respiratory | Head Development | Neural Crest Development | Head and Neural Crest | Musculoskeletal Development | Limb Development | Musculoskeletal | Renal Development | Genital | Kidney and Genital | Sensory | Stem Cells | Stem Cells | Endocrine Development | Endocrine | Heart | Integumentary Development | Heart and Integumentary | Fetal | Birth and Revision | Fetal
Glossary Links
Cite this page: Hill, M.A. (2024, April 19) Embryology Lecture - Stem Cells. Retrieved from https://embryology.med.unsw.edu.au/embryology/index.php/Lecture_-_Stem_Cells
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