Lecture - Respiratory Development: Difference between revisions

From Embryology
mNo edit summary
 
(91 intermediate revisions by 2 users not shown)
Line 1: Line 1:
== Introduction ==
{{Header}}
[[File:Gray0971.jpg|thumb|adult lungs]]
==Introduction==
[[File:Fetal head section 01.jpg|thumb|300px|Fetal Pharynx (week 12)]]
[[File:Respiratory tract.jpg|thumb|300px|Respiratory tree]]
 
The lecture will introduce the development of the respiratory system and associated structures. The respiratory system does not carry out its physiological function (of gas exchange) until after birth, though the respiratory tract, diaphragm and lungs do begin to form early in embryonic development and continue through fetal development, only functionally maturing just before birth. The lungs continue to grow postnatally through childhood and some research finding suggest that there remains potential for growth in the adult.
 
 
[[Media:2017 Lecture - Respiratory Development.pdf|'''2017 Lecture Slides''']]
 


The respiratory system does not carry out its physiological function (of gas exchange) until after birth. The respiratory tract, diaphragm and lungs do form early in embryonic development.
:''Current research suggests that both genetic and the developmental environment (fetal and postnatal) can influence the growth, differentiation and function of the respiratory system.''


{|
|
The respiratory tract is divided anatomically into 2 main parts:  
The respiratory tract is divided anatomically into 2 main parts:  
# '''upper respiratory tract''' - consisting of the nose, nasal cavity and the pharynx.
# '''upper respiratory tract''' - consisting of the nose, nasal cavity and the pharynx.
# '''lower respiratory tract''' - consisting of the larynx, trachea, bronchi and the lungs.  
# '''lower respiratory tract''' - consisting of the larynx, trachea, bronchi and the lungs.  
| [[File:Bronchi_lungs.jpg|200px|Lower respiratory tract]]
|}
The respiratory "system"  usually includes descriptions of not only the functional development of the lungs, but also related musculoskeletal (diaphragm) and vascular (pulmonary) development.


The respiratory "system"  usually includes descriptions of not only the functional development of the lungs, but also related musculoskeletal (diaphragm) and vascular (pulmonary) development.
===Key Concepts===
# upper and lower respiratory tract.
# Embryonic origin of respiratory components (tract, lungs, diaphragm, muscles).
# Key stages in respiratory development.
# Time course of respiratory development.
# Respiration at birth.
# Postnatal development of respiration.
# Developmental abnormalities.
 
{| class="wikitable mw-collapsible mw-collapsed"
! colspan=2| Textbooks  
|-
| [[File:Logo.png|80px]]
| {{Embryo citation}}


[https://embryology.med.unsw.edu.au/embryology/index.php?title=Lecture_-_Respiratory_Development&oldid=302824 2017 Lecture]


{{Respiratory Links}}
|-
| [[File:The Developing Human, 10th edn.jpg|90px]]
| {{MPT2015APAcitation}}
* Chapter 10  [http://ebookcentral.proquest.com.wwwproxy1.library.unsw.edu.au/lib/unsw/reader.action?docID=2074364&ppg=269 Respiratory System]
* Chapter 8 [http://ebookcentral.proquest.com.wwwproxy1.library.unsw.edu.au/lib/unsw/reader.action?docID=2074364&ppg=198 Body Cavities and Diaphragm]
* Chapter 9 [http://ebookcentral.proquest.com.wwwproxy1.library.unsw.edu.au/lib/unsw/reader.action?docID=2074364&ppg=216 Pharyngeal Apparatus, Face, and Neck]
|-
| [[File:Larsen's human embryology 5th ed.jpg|90px]]
| {{SBBF2015APAcitation}}
*  Chapter 11  [http://ebookcentral.proquest.com.wwwproxy1.library.unsw.edu.au/lib/unsw/reader.action?docID=2074524&ppg=269 Development of the Respiratory System and Body Cavities]
|-
| Additional Textbooks
|
* Histology and cell biology: An introduction to pathology  [http://www.unsw.eblib.com.wwwproxy0.library.unsw.edu.au/patron/Read.aspx?p=1430108&pg=404 Chapter 13. Respiratory System]
* Developmental Biology 11e Online [http://11e.devbio.com/wt2001.html Lung Induction]
* Before We Are Born (5th ed.) Moore and Persaud Chapter 13 p255-287
* Essentials of Human Embryology Larson Chapter 9 p123-146
* Human Embryology Fitzgerald and Fitzgerald Chapter 19, 20 p119-123
|}


'''Links:''' [[Respiratory System Development]]
==ECHO Lecture Recording==
[[User:Z8600021|Mark Hill]] ([[User talk:Z8600021|talk]]) 12:12, 7 October 2018 (AEDT)


==Lecture Objectives==
Due to lecturer illness, the lecture recording below is made available in place of the 2018 Lecture. It contains material similar to the respiratory theory information that would have been presented in the current series.
[[File:Lung_secondary_lobule_01.jpg|thumb|lung structure]]
[[File:Gray0975.jpg|thumb|alveoli and blood vessels]]
* Understanding of embryonic lung development
* Understanding of the stages of lung development
* Understanding of diaphragm development
* Brief understanding of respiratory vascular development
* Brief understanding of respiratory abnormalities
* Brief understanding of molecular mechanisms


{|
<html5media height="600" width="800">File:2014ANAT2341_Lecture_10_-_Respiratory Development.mp4</html5media>
| [[File:Podcast_icon.jpg|link=ANAT2341_Embryology_2012_Lecture_Recordings]]
| '''Lectopia Lecture Audio'''
[http://lectopia.telt.unsw.edu.au/lectopia/lectopia.lasso?ut=153&id=140221 Lecture 10 - Respiratory Development  Lecture Date: 2012-08-21  Lecture Time: 15:00 Venue: CLB 3 Speaker: Mark Hill


|}


==Textbooks==
==Respiratory Functional Unit ==
===The Developing Human: Clinically Oriented Embryology===
===Alveolus===
'''Alveolus''' (Latin ''alveolus'' = "little cavity", plural is alveoli)
{|
{|
| [[File:Respiratory_histology_03.jpg|400px]]
| [[File:Alveolar-sac-01.jpg|400px]]
|-
|-
| [[File:The Developing Human, 9th edn.jpg|90px]]
| Alveolus histology
| '''Citation:''' The Developing Human: clinically oriented embryology 9<sup>th</sup> ed. Keith L. Moore, T.V.N. Persaud, Mark G. Torchia. Philadelphia, PA: Saunders, 2011.
| Alveolus structure
* [http://er.library.unsw.edu.au/er/cgi-bin/eraccess.cgi?url=http://www.mdconsult.com/books/page.do?eid=4-u1.0-B978-1-4377-2002-0..00008-4&isbn=978-1-4377-2002-0&uniqId=330028653-2#4-u1.0-B978-1-4377-2002-0..00008-4 Chapter 8 – Body Cavities and Diaphragm]
* [http://er.library.unsw.edu.au/er/cgi-bin/eraccess.cgi?url=http://www.mdconsult.com/books/page.do?eid=4-u1.0-B978-1-4377-2002-0..00010-2&isbn=978-1-4377-2002-0&uniqId=330028653-2#4-u1.0-B978-1-4377-2002-0..00010-2 Chapter 10 – Respiratory System]
|}
|}
 
{|
===Larsen's Human Embryology===
| [[File:Lung_primary_lobule_01.jpg|300px]]
{| border='0px'
| [[File:Lung_secondary_lobule_01.jpg|300px]]
|-
|-
| [[File:Larsen's human embryology 4th edn.jpg|90px]]
| valign=top|'''Primary Lobule'''
| '''Citation:''' Larsen's human embryology 4th ed. Schoenwolf, Gary C; Larsen, William J, (William James). Philadelphia, PA : Elsevier/Churchill Livingstone, c2009.
* region supplied by a '''respiratory bronchiole'''
| '''Secondary Lobule'''
* region supplied by a '''terminal bronchiole'''  
* size -  up to 2.5 cm across.
* connective tissue - bounded by fibrous (interlobular) septa and containing internal (interlobular) septa.
* lobule contains a up to 12 acini and 30 - 50 [[:File:Lung primary lobule 01.jpg|primary lobules]].
* blood supply - pulmonary artery branch
* blood drainage - pulmonary veins located at lobule periphery leave though the interlobular septa.
* lymphatics - arterial and interlobular septa associated (drain to subpleural plexus).


* [http://er.library.unsw.edu.au/er/cgi-bin/eraccess.cgi?url=http://www.mdconsult.com/books/linkTo?type=bookPage&isbn=978-0-443-06811-9&eid=4-u1.0-B978-0-443-06811-9..10011-9 Chapter 11 - Development of the Respiratory System and Body Cavities]
|}
|}


===UNSW Embryology===
==Developmental Mechanisms==
{|
* '''Initiation''' - Budding of foregut [[endoderm]] to generate the trachea.
| [[File:Logo.png|90px]]
* '''Branching''' - A repeated mechanism of branching that is ongoing throughout development to form the conducting bronchioles then alveolar ducts.
| Hill, M.A. (2012) <i>UNSW Embryology</i> (12<sup>th</sup> ed.). Sydney:UNSW.
* '''Surface area increase''' - Expansion of the surface area in late development generating eventually the thin air–blood barrier for gas exchange in the acini.
{{Respiratory Links}}
* '''Vascular development''' - Extension of a vascular capillary tree within the connective tissue and wall of the acini for gas exchange, and the lymphatic development for immunology of the lungs.
|}
* '''Surfactant development''' -  allows lung inflation and decreases the work of breathing and also related to immunology of the lungs.
* '''Musculoskeletal development''' - contributes the mechanical elements of ribs, intercostals and [[Diaphragm Development|diaphragm]] required for breathing.
 
==Developmental Overview==
[[File:Lung_alveoli_development_cartoon.jpg|thumb|300px]]
Germ Layers
* Endoderm and splanchnic mesoderm form majority of conducting and alveoli.
* Ectoderm will contribute the neural innervation.
* Mesoderm also contributes the supporting musculoskeletal components.
 
'''Week 4''' - laryngotracheal groove forms on floor foregut.


'''Week 5''' - left and right lung buds push into the pericardioperitoneal canals (primordia of pleural cavity)


* '''Anatomy of the Human Body''' 1918 Henry Gray [[Anatomy_of_the_Human_Body_by_Henry_Gray#947_Respiratory|The Respiratory Apparatus]]
'''Week 6''' - descent of heart and lungs into thorax. Pleuroperitoneal foramen closes.
* '''Developmental Biology''' 8e Online [http://8e.devbio.com/article.php?ch=15&id=157 Lung Branching Morphogenesis]


==Developmental Overview==
'''Week 7''' - enlargement of liver stops descent of heart and lungs.
[[File:Stage14 respiratory tract.jpg|thumb|Week 5 Respiratory Development]]


===Lung Development===
'''Month 3-6''' - lungs appear glandular, end month 6 alveolar cells type 2 appear and begin to secrete surfactant.


* week 4 - 5 embryonic
'''Month 7''' - respiratory bronchioles proliferate and end in alveolar ducts and sacs.
* week 5 - 17 pseudoglandular
* week 16 - 25 canalicular
* week 24 - 40 terminal sac
* late fetal - 8 years alveolar


===Germ Layers===
{| class="wikitable mw-collapsible mw-collapsed"
* Endoderm and splanchnic mesoderm form majority of conducting and alveoli.
! Molecular Signaling &nbsp;
* Ectoderm will contribute the neural innervation.
|-
* Mesoderm also contributes the supporting musculoskeletal components.
| Early development is all about "branching". Fibroblast Growth Factors from the surrounding mesoderm acting through membrane receptors and Sox transcription factors are key regulators of early endodermal branching (Sox2 for initiation, Sox9 for tip maintenance and branching)
|-
| [[File:Lung human and mouse Sox expression.jpg|600px]]


===Events===
Human and mouse Sox expression<ref name=PMID28806170><pubmed>28806170</pubmed></ref>
* '''Week 4''' - laryngotracheal groove forms on floor foregut.
|-
* '''Week 5''' - left and right lung buds push into the pericardioperitoneal canals (primordia of pleural cavity)
| '''Bmp4''' acts as in an autocrine signalling mechanism to limit bud outgrowth.
* '''Week 6''' - descent of heart and lungs into thorax. Pleuroperitoneal foramen closes.
|}
* '''Week 7''' - enlargement of liver stops descent of heart and lungs.
* '''Month 3-6''' - lungs appear glandular, end month 6 alveolar cells type 2 appear and begin to secrete surfactant.
* '''Month 7''' - respiratory bronchioles proliferate and end in alveolar ducts and sacs.


==Lung Development Stages==
==Development Stages==
[[File:Lung_alveoli_development_cartoon.jpg|thumb|300px]]
Note - the sequence is important rather than the actual timing, which is variable in the existing literature.
The sequence is most important rather than the actual timing, which is variable in the existing literature.


# week 4 - 5 embryonic
{{lung stage table}}
# week 5 - 17 pseudoglandular
# week 16 - 25 canalicular
# week 24 - 40 terminal sac
# late fetal - 8 years alveolar


===Embryonic===
===Embryonic===
Week 4 to 5 - lung buds originate as an outgrowth from the ventral wall of the foregut where lobar division occurs.
{|
| colspan=2|[[File:Endoderm_cartoon.jpg|alt=Endoderm development cartoon|link=Endoderm_Development_Movie]]
|
|-
| [[File:Stage11_bf9.jpg|300px]]
| [[File:Stage11_sem4.jpg|300px]]
|-
| Stomodeum (Week 4, stage 11)
| Buccopharyngeal membrane  (Week 4, stage 11)
|}
[[File:Stage14 respiratory tract.jpg|300px|Week 5 Respiratory Development]]
(Week 5, stage 14)
* '''week 4 - 5'''  
* '''week 4 - 5'''  
* Endoderm - tubular ventral growth from foregut pharynx.
* Endoderm - tubular ventral growth from foregut pharynx.
* Mesoderm - mesenchyme of lung buds.
* Mesoderm - mesenchyme of lung buds.
* Intraembryonic coelom - pleural cavities elongated spaces connecting pericardial and peritoneal spaces.
* Intraembryonic coelom - pleural cavities elongated spaces connecting pericardial and peritoneal spaces.
[[Stage_13_MRI_Movie_1|Stage 13 - Trachea and Lung buds]]
{|
| [[File:Gray0982a.jpg|200px]]
| [[File:Bailey287.jpg|200px]]
| [[File:Bailey288.jpg|200px]]
| [[File:Bailey289.jpg|200px]]
|-
| Week 4
| Week 4-5 (Stage [[Carnegie_stage_12|12]] to [[Carnegie_stage_13|13]])
| Week 5 (Stage [[Carnegie_stage_15|15]] to [[Carnegie_stage_16|16]])
| Week 6 (Stage [[Carnegie_stage_16|16]] to [[Carnegie_stage_17|17]])
|}
===Pseudoglandular stage===
===Pseudoglandular stage===
{|
|
* '''week 5 - 17'''  
* '''week 5 - 17'''  
* tubular branching of the human lung airways continues  
* tubular branching of the human lung airways continues  
Line 109: Line 185:
** more distal structures are lined with '''cuboidal epithelium'''.
** more distal structures are lined with '''cuboidal epithelium'''.


* Pulmonary neuroendocrine cells (PNECs) - develop in late embryonic to early fetal period, later in mid-fetal period clusters of these cells form neuroepithelial bodies (NEBs) in airway epithelium. May stimulate mitosis to increase branching, secrete 2 peptides - gastrin-releasing peptide (GRP) and calcitonin gene related peptide (CGRP)
[[File:ME54 001.jpg|400px]]
Week 8
| [[File:Fetal lung histology.jpg|300px]]
Fetal lung histology
|}
([[:File:Parotid gland histology 06.jpg|This is what a gland looks like.]])
===Canalicular stage===
===Canalicular stage===
 
{|
|
* '''week 16 - 24'''  
* '''week 16 - 24'''  
* Lung morphology changes dramatically  
* Lung morphology changes dramatically  
Line 116: Line 204:
* '''Surfactant''' synthesis and the canalization of the lung parenchyma by capillaries begin.  
* '''Surfactant''' synthesis and the canalization of the lung parenchyma by capillaries begin.  
* future gas exchange regions can be distinguished from the future conducting airways of the lungs.
* future gas exchange regions can be distinguished from the future conducting airways of the lungs.
 
| [[File:Lung - canalicular stage.jpg|400px]]
|}
===Saccular stage===
===Saccular stage===
[[File:Alveolar-sac-01.jpg|thumb|Alveolar sac structure]]
[[File:Alveolar-sac-01.jpg|thumb|300px|Alveolar sac structure]]
* '''week 24 to near term.'''  
* '''week 24 to near term.'''  
* most peripheral airways form widened "airspaces", termed saccules.  
* most peripheral airways form widened "airspaces", termed '''saccules'''.  
* saccules widen and lengthen the airspace (by the addition of new generations).  
* saccules widen and lengthen the airspace (by the addition of new generations).  
* future gas exchange region expands significantly.  
* future gas exchange region expands significantly.  
* Fibroblastic cells also undergo differentiation, they produce extracellular matrix, collagen, and elastin.  
* Fibroblastic cells also undergo differentiation, they produce extracellular matrix, collagen, and elastin.
** May have a role in epithelial differentiation and control of surfactant secretion.
** May have a role in epithelial differentiation and control of '''surfactant secretion'''.
* The vascular tree also grows in length and diameter during this time.
* Alveolar Cells Type II (Type II pneumocytes)
** begin to secrete '''surfactant''', levels of secretion gradually increase to term.
** allows alveoli to remain inflated
* Vascular tree - also grows in length and diameter during this time.


==Foregut development==
===Alveolar stage===
[[File:Head arches cartoon.jpg|thumb|Foregut cartoon]]
[[File:Respiratory secondary septum 01.jpg|thumb|300px|Secondary septum]]
From the oral cavity the next portion of the foregut is initially a single gastrointestinal (oesophagus) and respiratory (trachea) common tube, the pharynx which lies behind the heart. Note that the respiratory tract will form from a ventral bud arising at this level.
{|
|
* late fetal to 8 years.
* Secondary septum
* Expansion of gas exchange alveoli, vascular beds (capillaries), lymphatics and innervation.
* Postnatal lung, with '''alveoli''' forming.
| [[File:Postnatal_alveoli_number.jpg|400px]]
|}


* Oral cavity
==Upper Respiratory Tract==
* Pharynx (esophagus, trachea)
{|
* Respiratory tract
| [[File:Head arches cartoon.jpg|300px]]
* Stomach
| [[File:Pharynx_cartoon.jpg|300px]]
|-
| Foregut cartoon
| Pharynx
|}


==Upper respiratory tract==
'''Foregut Development''' - from the oral cavity the next portion of the foregut is initially a single gastrointestinal (oesophagus) and respiratory (trachea) common tube, the pharynx which lies behind the heart. Note that the respiratory tract will form from a ventral bud arising at this level.
[[File:Gray0961.jpg|thumb|Adult upper respiratory tract conducting system]]
{|
| [[File:Pharynx.jpg|Pharynx|400px]]
|
* part of '''foregut''' development  (Oral cavity, Pharynx (esophagus, trachea), Respiratory tract, Stomach)
* anatomically the nose, nasal cavity and the pharynx
* '''pharynx''' forms a major arched cavity within the pharyngeal arches ('''MH''' - pharyngeal arches will be described in BGD head development lecture).
* '''palate''' - development for mammals, allows breathing while feeding.
 
Note - Specialised '''olfactory epithelium''' for smell, a small region located in roof of nasal cavity.
|-
| [[File:Respiratory histology 11.jpg|300px]]
| valign=top|
'''Respiratory epithelium'''
* pseudo-stratified
* ciliated cells
* goblet cells
* basal cells
 
[[File:Respiratory epithelium cells cartoon.jpg|200px]]
 
Respiratory epithelium development
 
|}
<gallery>
<gallery>
File:Gitbpm.jpg|stage 11 foregut
File:Gray0982a.jpg|week 4 early respiratory endodermal bud
File:Stage_22_image_167.jpg|Stage 22 trachea
File:Head_arches_cartoon.jpg|Head arches cartoon
File:Pharynx_cartoon.jpg|Pharynx
File:Nasal cavities.jpg|Nasal cavities
File:Nasal cavities.jpg|Nasal cavities
File:Pharynx.jpg|Pharynx
File:Larynx.jpg|Larynx
File:Larynx.jpg|Larynx
File:Gray0961.jpg|Adult upper respiratory tract conducting system
</gallery>
</gallery>
* part of foregut development
* anatomically the nose, nasal cavity and the pharynx
* the pharynx forms a major arched cavity within the pharyngeal arches


'''MH''' - pharyngeal arches will be described in head development lecture


==Lower respiratory tract==
{| class="wikitable mw-collapsible mw-collapsed"
!  Additional Information - Histology
|-
| This will be covered in detail in your associated SH Practical class.
<gallery>
File:Respiratory histology 13.jpg|Olfactory Epithelium
File:Respiratory histology 14.jpg|Olfactory Epithelium
File:Respiratory histology 11.jpg|Respiratory Epithelium
File:Respiratory histology 12.jpg|Respiratory Epithelium
</gallery>
 
{|
|-
| valign=top width=380px|'''Olfactory epithelium'''
* Olfactory cells
* Sustentacular cells - located mainly in the superficial cell layer of the epithelium (difficult to distinguish from olfactory cells).
* Basal cells - identified by their location in the epithelium.
 
'''Epithelium'''
* Cilia are not visible
* goblet cells are absent from the olfactory epithelium.
 
'''Lamina Propria'''
* olfactory axon bundles (lightly stained, rounded areas) connected to olfactory cells.
* Bowman's glands - (small mucous glands, olfactory glands) function to moisturise the epithelium.
 
{{Nasal olfactory links}}
 
| valign=top width=380px|'''Respiratory epithelium'''
* goblet cells
* ciliated cells
* basal cells
 
'''Lamina propria'''
* connective tissue
* cavernous sinusoids - large spaces (empty or filled with red blood cells)
* glandular tissue - mucous glands (green) and muco-serous glands (brownish-green)
 
'''Bone'''
* Lamellae and osteocytes in lacunae.
* Haversian systems are rare or absent.
 
{{Nasal respiratory links}}
|}
 
{{Respiratory Histology}}
 
|}
 
==Lower Respiratory Tract==
<gallery>
<gallery>
File:Gray0982a.jpg|week 4 early respiratory endodermal bud
File:Gray0982a.jpg|week 4 early respiratory endodermal bud
Line 161: Line 326:
File:Respiratory tract.jpg|conducting system bronchi to lungs
File:Respiratory tract.jpg|conducting system bronchi to lungs
</gallery>
</gallery>
[[File:Lung_development_stage13-22.jpg]] [[File:Stage_22_image_171.jpg|300px]]
{|
| [[File:Lung_development_stage13-22.jpg]]
| [[File:Stage_22_image_171.jpg|300px]]
|-
| [[Respiratory_System_-_Carnegie_Stage_13|Stage 13 (Week 4-5)]]
| [[Respiratory_System_-_Carnegie_Stage_22|Stage 22 (Week 8)]]
|}


[[File:Lung alveoli development cartoon.jpg|thumb|Lung alveoli development cartoon]]
[[File:Lung alveoli development cartoon.jpg|thumb|Lung alveoli development cartoon]]
[[File:Fetal lung histology.jpg|thumb|Fetal lung histology]]


* lung buds ( endoderm epithelial tubes) grow/push into mesenchyme covered with pleural cells (lung border)
* lung buds ( endoderm epithelial tubes) grow/push into mesenchyme covered with pleural cells (lung border)
Line 171: Line 341:
# terminal bifurcation
# terminal bifurcation
# lateral budding
# lateral budding
* The lungs go through an embryonic and 4 distinct histological phases of development


Growth initially of branched "conducting" system of bronchial tree, followed by later development of the "functional units" of the alveoli.
Growth initially of branched "conducting" system of bronchial tree, followed by later development of the "functional units" of the alveoli.


* '''embryonic''' - week 4 - 5 (stage 14 above)
{| class="wikitable mw-collapsible mw-collapsed"
* '''pseudoglandular''' - week 5 - 17  (stage 22 above)
!  Additional Information - Histology
*  '''canalicular''' - week 16 - 25
|-
*  '''terminal sac''' - week 24 - 40
| This will be covered in detail in your associated SH Practical class.
*  '''alveolar''' - late fetal - 8 years (Latin, ''alveus'' = cavity or hollow)
'''Respiratory Trachea'''


'''Mucosa''' - formed by epithelium and underlying lamina propria.
* respiratory epithelium - (pseudostratified columnar and ciliated) ciliated cells, goblet cells, brush cells, endocrine cells, surfactant-producing cells (Clara cells), serous cells, basal cells, basement membrane.
* lamina propria - loose connective tissue, many elastic fibres


===Fetal lung volume===
'''Submucosa''' - connective tissue and submucosal glands
* submucosal glands (both serous and mucous parts)
 
'''Cartilage'''
* perichondrium
* tracheal cartilage - hyaline cartilage, 16 to 20 C-shaped cartilages.
* trachealis muscle - (smooth muscle) Not visible in this section, together with connective tissue fibres, join ends of the cartilages together.
 
'''Hyaline Cartilage Development'''
* forms from mesenchymal cells.
* precursor cells become rounded and form densely packed cellular masses, chondrification centres.
* chondroblasts - (cartilage-forming cells) begin secreting the extracellular matrix components of cartilage.
** extracellular matrix - ground substance (hyaluronan, chondroitin sulfates and keratan sulfate) and tropocollagen (polymerises into fine collagen fibres, not visible).
 
<gallery>
File:Hyaline_cartilage_03.jpg|Trachea (overview HE)
File:Hyaline_cartilage_04.jpg|Trachea (overview VG)
File:Respiratory histology 05.jpg|Trachea (detail layers)
File:Respiratory histology 06.jpg|Trachea (detail glands)
</gallery>
 
'''Bronchi Branching'''
 
main bronchi -> lobar bronchi -> segmental bronchi (supply lung bronchopulmonary segments) -> bronchi -> bronchioles (smaller than 1 mm) -> '''respiratory bronchioles'''.
 
* Trachea branches into 2 '''main bronchi''', with a histological structure similar to that of the trachea.
* branches are accompanied by branches of the pulmonary artery, nerves and lymph vessels
*  surrounded by a layer of smooth muscle, which is located between the cartilage and epithelium.
 
'''Bronchioles'''
* transition from bronchi to bronchioles the epithelium changes to a '''ciliated columnar epithelium'''.
* Smooth muscle present, glands and cartilage are absent.
 
'''Respiratory Bronchioles'''
 
* first structures that belong to the respiratory portion of the respiratory system.
* wall out-pouchings form alveoli (site of gas exchange)
* end in alveolar ducts
* alveoli - duct or sac.
 
<gallery>
File:Respiratory_histology_01.jpg|Bronchiole
File:Respiratory histology 10.jpg|Lung Elastin
File:Respiratory histology 08.jpg|labeled lung
</gallery>
{|
|-
| valign=top width=350px|'''Alveolar type I cells'''
* small alveolar cells or type I pneumocytes
* are extremely flattened (the cell may be as thin as 0.05 µm)
* form the bulk (95%) of the surface of the alveolar walls.
* The shape of the cells is very complex, and they may actually form part of the epithelium on both faces of the alveolar wall.
| valign=top width=350px|'''Alveolar type II cells'''
* large alveolar cells or type II pneumocytes
* about as many type II cells as type I cells (cell shape accounts for small contribution to alveolar area).
* irregularly (sometimes cuboidal) shaped.
* form small bulges on the alveolar walls.
* contain are large number of granules called cytosomes (or multilamellar bodies)
** consist of precursors to pulmonary surfactant (mixture of phospholipids that keep surface tension in the alveoli low).
|}
 
<gallery>
File:Respiratory_histology_02.jpg|Alveolar Duct
File:Respiratory_histology_03.jpg|Alveoli
File:Respiratory histology 04.jpg|Alveoli Elastin
</gallery>
 
{{Respiratory Histology}}
 
|}
===Fetal Lung Volume===
Each human lung volume as determined by ultrasound and matched to gestational age <ref><pubmed>16388511</pubmed></ref>
Each human lung volume as determined by ultrasound and matched to gestational age <ref><pubmed>16388511</pubmed></ref>
{|
{|
|
{|
|-bgcolor="CEDFF2"
| Weeks (gestational)
| Weeks (gestational)
| Volume (ml)
| Volume (ml)
Line 190: Line 436:
| 12 to 13
| 12 to 13
| 0.05
| 0.05
|-
|-bgcolor="F5FAFF"
| 19 to 22
| 19 to 22
| 0.5
| 0.5
Line 196: Line 442:
| 29 to 32
| 29 to 32
| 1.9
| 1.9
|}
| [[File:Lung volume graph 01.jpg|200px]]
|}
|}


Line 201: Line 449:
[[File:Gray0965.jpg|thumb|pleura]]
[[File:Gray0965.jpg|thumb|pleura]]
[[File:Gray0968.jpg|thumb|pleura]]
[[File:Gray0968.jpg|thumb|pleura]]
* The anatomical body cavity in which the lungs develop and lie.  
* anatomical body cavity in which the lungs develop and lie.  
* The pleural cavity forms in the lateral plate mesoderm as part of the early single intraembryonic coelom.  
* pleural cavity forms in the '''lateral plate mesoderm''' as part of the early single '''intraembryonic coelom'''.  
* This cavity is initially continuous with pericardial and peritoneal cavities and form initially as two narrow canals
* This cavity is initially continuous with pericardial and peritoneal cavities and form initially as two narrow canals.
** later becomes separated by folding (pleuropericardial fold, pleuroperitoneal membrane) and the later formation of the diaphragm
** later becomes separated by folding (pleuropericardial fold, pleuroperitoneal membrane) and the later formation of the diaphragm.
 
pleuropericardial fold - (pleuropericardial membrane) An early embryonic fold which restricts the communication between pleural cavity and pericardiac cavity, contains both the cardinal vein and phrenic nerve.
 
pleuroperitoneal membrane - An early embryonic membrane that forms inferiorly at the septum transversum to separate peritoneal cavity from pleural cavity.


* '''pleuropericardial fold''' - (pleuropericardial membrane) An early embryonic fold which restricts the communication between pleural cavity and pericardiac cavity, contains both the cardinal vein and phrenic nerve.
* '''pleuroperitoneal membrane''' - An early embryonic membrane that forms inferiorly at the septum transversum to separate peritoneal cavity from pleural cavity.


===Pleura===
===Pleura===
* serous membrane covers the surface of the lung and the spaces between the lobes
* '''serous membrane''' covers the surface of the lung and the spaces between the lobes.
* arranged as a closed invaginated sac
* arranged as a closed invaginated sac.
* two layers (pulmonary, parietal) continuous with each other, the potential space between them is the '''pleural cavity'''
* two layers ('''pulmonary''', '''parietal''') continuous with each other, the potential space between them is the '''pleural cavity'''.


==Diaphragm==
==Diaphragm==
Line 227: Line 473:
# pleuroperitoneal membranes- connective tissue around central tendon
# pleuroperitoneal membranes- connective tissue around central tendon
|}
|}
[[File:Gray804.gif|thumb|Adult Cervical Plexus (phrenic nerve shown lower right)]]
[[File:Gray0804.jpg|thumb|Adult Cervical Plexus (phrenic nerve shown lower right)]]
[[File:Gray0391.jpg|300px|adult diaphragm]]
[[File:Gray0391.jpg|300px|adult diaphragm]]


Line 235: Line 481:
** motor neurons for the diaphragm
** motor neurons for the diaphragm
** sensory nerves for other abdominal structures (mediastinum, pleura, liver, gall bladder).
** sensory nerves for other abdominal structures (mediastinum, pleura, liver, gall bladder).
'''Bochdalek hernia''' -  most common on the posterior left side (85%). Failure of the pleuroperitoneal foramen (foramen of Bochdalek) to close allows viscera into thorax. Intestine, stomach or spleen can enter the pleural cavity, compressing the lung.


==Pulmonary Circulation==  
==Pulmonary Circulation==  
Line 246: Line 494:
===Fetal Respiratory Movements===
===Fetal Respiratory Movements===
* Fetal respiratory movements (FRM) or Fetal breathing movements (FBM) are regular muscular contrations occurring in the third trimester.  
* Fetal respiratory movements (FRM) or Fetal breathing movements (FBM) are regular muscular contrations occurring in the third trimester.  
* thought to be preparing the respiratory muscular system for neonatal function
* preparing the respiratory muscular system for neonatal function.
thought to also have a role in late lung development.
may also have a role in late lung development.


==The First Breath==
==The First Breath==
Line 261: Line 509:
* blood fills the alveolar capillaries
* blood fills the alveolar capillaries


In the heart, pressure in the right side of the heart decreases and pressure in the left side of the heart increases (more blood returning from pulmonary).
In the heart - pressure in the right side of the heart decreases and pressure in the left side of the heart increases (more blood returning from pulmonary).
==Postnatal==
[[File:Postnatal alveoli number.jpg|thumb|300px|Postnatal alveoli number]]
[[File:Neonatal rib orientation.jpg|thumb|Rib orientation]]
[[File:Neonatal rib orientation.jpg|thumb|Rib orientation]]
* Respiratory Rate is higher than adult (30 breaths/minute).
===Alveoli===
* At birth about 15% of adult alveoli number have formed
** 20 - 50 million to in the adult about 300 million.
* remaining subdivisions develop in the first few postnatal years
 
[[:File:Postnatal_alveoli_number.jpg|Alveoli Number]]
 
===Respiratory Rate===
* neonatal rate is higher (30-60 breaths/minute) than adult (12-20 breaths/minute).
** tachypnea - (Greek, rapid breathing) an increased respiratory rate of greater than 60 breaths/minute in a quiet resting baby
{|
|-bgcolor="CEDFF2"
| width= "300px"|'''Age'''
| width= "200px"|'''Rate''' (breaths/minute)
|-
| Infant (birth - 1 year)
| 30 - 60
|- bgcolor="F5FAFF"
| Toddler (1 - 3 years)
| 24 - 40
|-
| Preschool (3 - 6 years)
| 22 - 34
|-bgcolor="F5FAFF"
| School age (6 - 12 years)
| 18 - 30
|-
| Adolescent (12 - 18 years)
| 12 - 16
|-bgcolor="F5FAFF"
|
|}


===Rib Orientation===
===Rib Orientation===
* Infant rib - is virtually horizontal, allowing diaphragmatic breathing only.  
 
* Adult rib - is oblique (both anterior and lateral views), allows for pump-handle and bucket handle types of inspiration.
* Infant rib - is virtually '''horizontal''', allowing diaphragmatic breathing only.  
* Adult rib - is '''oblique''' (both anterior and lateral views), allows for pump-handle and bucket handle types of inspiration.


== Respiratory Tract Abnormalities ==
== Respiratory Tract Abnormalities ==
[[File:Human congenital diaphragmatic hernia.jpg|thumb|Congenital diaphragmatic hernia]]
[[File:Lung_Azygos_Lobe_02.jpg|thumb|Lung Azygos Lobe]]
[[File:Preterm delivery and lung development.jpg|thumb|Preterm Birth]]
[[Respiratory System - Abnormalities]]
[[Respiratory System - Abnormalities]]
===Tracheoesophageal Fistula ===
(Tracheo-Oesophageal Fistula, Oesophageal Atresia) - Oesophageal Atresia with or without tracheo-oesophageal fistula


===Lobar Emphysema (Overinflated Lung)===
* '''Meconium Aspiration Syndrome''' - (MAS) Meconium is the gastrointestinal contents that accumulate in the intestines during the fetal period. Fetal stress in the third trimester, prior to/at/ or during parturition can lead to premature meconium discharge into the amniotic fluid and sunsequent ingestion by the fetus and damage to respiratory function. Damage to placental vessels meconium myonecrosis may also occur.
# There is an overinflated left upper lobe
 
# There is a collapsed lower lobe
* '''Newborn Respiratory Distress Syndrome''' - (Hyaline Membrane Disease) membrane-like substance from damaged pulmonary cells,  absence of surfactant, if prolonged can be irreversible, intrauterine asphyxia, prematurity and maternal diabetes [http://www.nlm.nih.gov/MEDLINEPLUS/ency/article/001563.htm medline plus] | [http://www.medscape.com/article/976034-overview eMedicine]
# The left lung is herniating across the mediastinum
 
* '''Tracheoesophageal Fistula''' - Tracheo-Oesophageal Fistula, Oesophageal Atresia - Oesophageal Atresia with or without tracheo-oesophageal [[F#fistula|fistula]] '''Fistula''' - an abnormal communication between 2 structures (organs, vessels, cavities) that do not normally connect.
 
* '''Lobar Emphysema''' (Overinflated Lung) - There is an overinflated left upper lobe There is a collapsed lower lobe The left lung is herniating across the mediastinum
 
* '''Congenital Diaphragmatic Hernia''' - (1 in 3,000 live births) Failure of the pleuroperitoneal foramen (foramen of Bochdalek) to close (left side), allows viscera into thorax -iIntestine, stomach or spleen can enter the pleural cavity, compressing the lung. rare (Morgagni hernia) -an opening in the front of the diaphragm. [[Respiratory_System_-_Abnormalities#Congenital_Diaphragmatic_Hernia|Congenital Diaphragmatic Hernia]] | [http://www.ncbi.nlm.nih.gov/books/NBK1359 GeneReviews]
 
* '''Azygos Lobe''' - Common condition (0.5% of population). The right lung upper lobe expands either side of the posterior cardinal. There is also some course variability of the phrenic nerve in the presence of an azygos lobe.
 
* '''Congenital Laryngeal Webs''' - Laryngeal abnormality due to embryonic (week 10) incomplete recanalization of the laryngotracheal tube during the fetal period. Rare abnormality occuring mainly at the level of the vocal folds (glottis).
 
* '''Hyaline Membrane Disease''' - (Newborn Respiratory Distress Syndrome) a membrane-like substance from damaged pulmonary cells.
* '''Bronchopulmonary Dysplasia''' - A chronic lung disease which can occur following premature birth and related lung injury. Most infants who develop BPD are born more than 10 weeks before their due dates, weigh less than 1,000 grams (about 2 pounds) at birth, and have breathing problems.
 
*  '''Asthma''' - Flow limitation during tidal expiration in early life significantly associated with the development of physician-diagnosed asthma by the age of 2 years. Infants with abnormal lung function soon after birth may have a genetic predisposition to asthma or other airway abnormalities that predict the risk of subsequent lower respiratory tract illness. PMID 8176553
* '''Cystic Fibrosis''' - Inherited disease of the mucus and sweat glands, causes mucus to be thick and sticky. Clogging the lungs, causing breathing problems and encouraging bacterial grow. (Covered elsewhere in the course)
* '''Environmental Factors''' see recent review below. <pubmed>20444669</pubmed>
 
==Additional Information==


===Congenital Diaphragmatic Hernia===
{{Respiratory terms}}
Failure of the pleuroperitoneal foramen (foramen of Bochdalek) to close allows viscera into thorax. Intestine, stomach or spleen can enter the pleural cavity, compressing the lung.


===Azygos Lobe===
[[File:Lung_Azygos_Lobe_02.jpg|thumb|Lung Azygos Lobe]]
* Common condition (0.5% of population).
* The right lung upper lobe expands either side of the posterior cardinal.
* There is also some course variability of the phrenic nerve in the presence of an azygos lobe.


===Congenital Laryngeal Webs===
[[File:Gray0971.jpg|thumb|adult lungs]]
* Laryngeal abnormality due to embryonic (week 10) incomplete recanalization of the laryngotracheal tube during the fetal period.  
 
* Rare abnormality occuring mainly at the level of the vocal folds (glottis).
{| class="wikitable mw-collapsible mw-collapsed"
!  Grays - Respiratory Images &nbsp;
|-
|
<gallery>
File:Gray0947.jpg|947 The head and neck human embryo thirty-two days seen from the ventral surface.
File:Gray0948.jpg|948 Lung buds from a human embryo of about four weeks, showing commencing lobulations.
File:Gray0949.jpg|949 Lungs of a human embryo more advanced in development than week 4.
File:Gray0950.jpg|950 Cartilages of the larynx
File:Gray0950 epiglottis cartilage.jpg|950 epiglottis  cartilage
File:Gray0950 thyroid cartilage.jpg|950 thyroid cartilage
File:Gray0950 cricoid cartilage.jpg|950 cricoid cartilage
File:Gray0950 arytenoid cartilage.jpg|950 arytenoid cartilage
File:Gray0951.jpg|951 Ligaments of the larynx (anterior view)
File:Gray0952.jpg|952 Ligaments of the larynx (posterior view)
File:Gray0953.jpg|953 Larynx and upper part of the trachea
File:Gray0954.jpg|954
File:Gray0955.jpg|955 Larynx entrance
File:Gray0956.jpg|956
File:Gray0957.jpg|957
File:Gray0958.jpg|958
File:Gray0959.jpg|959
File:Gray0960.jpg|960
File:Gray0961.jpg|961 Cartilages of larynx, trachea, and bronchi (front view)
File:Gray0962.jpg|962 Bronchi and bronchioles
File:Gray0963.jpg|963
File:Gray0964.jpg|964
File:Gray0965.jpg|965
File:Gray0966.jpg|966 Lateral view of thorax, showing the relations of the pleuræ and lungs to the chest wall. Pleura in blue; lungs in purple.
File:Gray0967.jpg|967 Transverse section through the upper margin of the second thoracic vertebra.
File:Gray0968.jpg|968
File:Gray0969.jpg|969
File:Gray0970.jpg|970 Front view of heart and lungs
File:Gray0971.jpg|971 Adult lungs
File:Gray0974.jpg|974 Lung secondary lobule
File:Lung_secondary_lobule_01.jpg|974 relabeled version
File:Gray0975.jpg|975 Lung primary lobule
File:Lung_primary_lobule_01.jpg|975 relabeled version
File:Gray0976.jpg|976 Pig embryo lung
</gallery>
|}
 
{| class="wikitable mw-collapsible mw-collapsed"
!  Respiratory Histology
|-
| Fetal Histology
<gallery>
File:Fetal lung histology 02.jpg|Hyaline cartilage
File:Fetal lung histology.jpg|late canalicular
File:Fetal lung histology 01.jpg|unlabeled late canalicular
</gallery>
 
{{Fetal_Respiratory_Histology}}
|-
| Adult Histology
<gallery>
File:Respiratory histology 13.jpg|Olfactory Epithelium
File:Respiratory histology 14.jpg|Olfactory Epithelium
File:Respiratory histology 11.jpg|Respiratory Epithelium
File:Respiratory histology 12.jpg|Respiratory Epithelium
File:Respiratory histology 05.jpg|Trachea 1
File:Respiratory histology 06.jpg|Trachea 2
File:Respiratory_histology_01.jpg|Bronchiole
File:Respiratory histology 10.jpg|Lung Elastin
File:Respiratory histology 08.jpg|labeled lung
File:Respiratory_histology_02.jpg|Alveolar Duct
File:Respiratory_histology_03.jpg|Alveoli
File:Respiratory histology 04.jpg|Alveoli Elastin
</gallery>
|-
| {{Respiratory_Histology}}
|}
 


===Meconium Aspiration Syndrome===
<references/>


* (MAS) Meconium is the gastrointestinal contents that accumulate in the intestines during the fetal period.
* Fetal stress in the third trimester, prior to/at/ or during parturition can lead to premature meconium discharge into the amniotic fluid.
* Subsequent ingestion by the fetus and damage to respiratory function.
* Damage to placental vessels '''meconium myonecrosis''' may also occur.


===Newborn Respiratory Distress Syndrome===
* (Hyaline Membrane Disease) [http://www.nlm.nih.gov/MEDLINEPLUS/ency/article/001563.htm medline plus] | [http://www.medscape.com/article/976034-overview eMedicine]


===Bronchopulmonary Dysplasia===
* A chronic lung disease which can occur following premature birth.
* The definition of bronchopulmonary dysplasia (BPD) has in recent years changed.
* From a severe lung injury and associated repair, to more of a disruption of lung development.


{{Glossary}}
{{Glossary}}
{{Footer}}


{{2018ANAT2341}}
[[Category:Endoderm]] [[Category:Respiratory]]
[[Category:Endoderm]] [[Category:Respiratory]]

Latest revision as of 12:14, 7 October 2018

Embryology - 28 Mar 2024    Facebook link Pinterest link Twitter link  Expand to Translate  
Google Translate - select your language from the list shown below (this will open a new external page)

العربية | català | 中文 | 中國傳統的 | français | Deutsche | עִברִית | हिंदी | bahasa Indonesia | italiano | 日本語 | 한국어 | မြန်မာ | Pilipino | Polskie | português | ਪੰਜਾਬੀ ਦੇ | Română | русский | Español | Swahili | Svensk | ไทย | Türkçe | اردو | ייִדיש | Tiếng Việt    These external translations are automated and may not be accurate. (More? About Translations)

Introduction

Fetal Pharynx (week 12)
Respiratory tree

The lecture will introduce the development of the respiratory system and associated structures. The respiratory system does not carry out its physiological function (of gas exchange) until after birth, though the respiratory tract, diaphragm and lungs do begin to form early in embryonic development and continue through fetal development, only functionally maturing just before birth. The lungs continue to grow postnatally through childhood and some research finding suggest that there remains potential for growth in the adult.


2017 Lecture Slides


Current research suggests that both genetic and the developmental environment (fetal and postnatal) can influence the growth, differentiation and function of the respiratory system.

The respiratory tract is divided anatomically into 2 main parts:

  1. upper respiratory tract - consisting of the nose, nasal cavity and the pharynx.
  2. lower respiratory tract - consisting of the larynx, trachea, bronchi and the lungs.
Lower respiratory tract

The respiratory "system" usually includes descriptions of not only the functional development of the lungs, but also related musculoskeletal (diaphragm) and vascular (pulmonary) development.

Key Concepts

  1. upper and lower respiratory tract.
  2. Embryonic origin of respiratory components (tract, lungs, diaphragm, muscles).
  3. Key stages in respiratory development.
  4. Time course of respiratory development.
  5. Respiration at birth.
  6. Postnatal development of respiration.
  7. Developmental abnormalities.
Textbooks  
Logo.png Hill, M.A. (2020). UNSW Embryology (20th ed.) Retrieved March 28, 2024, from https://embryology.med.unsw.edu.au

2017 Lecture

Respiratory Links: respiratory | Science Lecture | Lecture Movie | Med Lecture | Stage 13 | Stage 22 | upper respiratory tract | diaphragm | Histology | Postnatal | respiratory abnormalities | Respiratory Quiz | Respiratory terms | Category:Respiratory
Historic Embryology - Respiratory 
1902 The Nasal Cavities and Olfactory Structures | 1906 Lung | 1912 Upper Respiratory Tract | 1912 Respiratory | 1913 Prenatal and Neonatal Lung | 1914 Phrenic Nerve | 1918 Respiratory images | 1921 Respiratory | 1922 Chick Pulmonary Vessels | 1934 Right Fetal Lung | 1936 Early Human Lung | 1937 Terminal Air Passages | 1938 Human Histology
The Developing Human, 10th edn.jpg Moore, K.L., Persaud, T.V.N. & Torchia, M.G. (2015). The developing human: clinically oriented embryology (10th ed.). Philadelphia: Saunders.
Larsen's human embryology 5th ed.jpg Schoenwolf, G.C., Bleyl, S.B., Brauer, P.R., Francis-West, P.H. & Philippa H. (2015). Larsen's human embryology (5th ed.). New York; Edinburgh: Churchill Livingstone.
Additional Textbooks
  • Histology and cell biology: An introduction to pathology Chapter 13. Respiratory System
  • Developmental Biology 11e Online Lung Induction
  • Before We Are Born (5th ed.) Moore and Persaud Chapter 13 p255-287
  • Essentials of Human Embryology Larson Chapter 9 p123-146
  • Human Embryology Fitzgerald and Fitzgerald Chapter 19, 20 p119-123

ECHO Lecture Recording

Mark Hill (talk) 12:12, 7 October 2018 (AEDT)

Due to lecturer illness, the lecture recording below is made available in place of the 2018 Lecture. It contains material similar to the respiratory theory information that would have been presented in the current series.

<html5media height="600" width="800">File:2014ANAT2341_Lecture_10_-_Respiratory Development.mp4</html5media>


Respiratory Functional Unit

Alveolus

Alveolus (Latin alveolus = "little cavity", plural is alveoli)

Respiratory histology 03.jpg Alveolar-sac-01.jpg
Alveolus histology Alveolus structure
Lung primary lobule 01.jpg Lung secondary lobule 01.jpg
Primary Lobule
  • region supplied by a respiratory bronchiole
Secondary Lobule
  • region supplied by a terminal bronchiole
  • size - up to 2.5 cm across.
  • connective tissue - bounded by fibrous (interlobular) septa and containing internal (interlobular) septa.
  • lobule contains a up to 12 acini and 30 - 50 primary lobules.
  • blood supply - pulmonary artery branch
  • blood drainage - pulmonary veins located at lobule periphery leave though the interlobular septa.
  • lymphatics - arterial and interlobular septa associated (drain to subpleural plexus).

Developmental Mechanisms

  • Initiation - Budding of foregut endoderm to generate the trachea.
  • Branching - A repeated mechanism of branching that is ongoing throughout development to form the conducting bronchioles then alveolar ducts.
  • Surface area increase - Expansion of the surface area in late development generating eventually the thin air–blood barrier for gas exchange in the acini.
  • Vascular development - Extension of a vascular capillary tree within the connective tissue and wall of the acini for gas exchange, and the lymphatic development for immunology of the lungs.
  • Surfactant development - allows lung inflation and decreases the work of breathing and also related to immunology of the lungs.
  • Musculoskeletal development - contributes the mechanical elements of ribs, intercostals and diaphragm required for breathing.

Developmental Overview

Lung alveoli development cartoon.jpg

Germ Layers

  • Endoderm and splanchnic mesoderm form majority of conducting and alveoli.
  • Ectoderm will contribute the neural innervation.
  • Mesoderm also contributes the supporting musculoskeletal components.

Week 4 - laryngotracheal groove forms on floor foregut.

Week 5 - left and right lung buds push into the pericardioperitoneal canals (primordia of pleural cavity)

Week 6 - descent of heart and lungs into thorax. Pleuroperitoneal foramen closes.

Week 7 - enlargement of liver stops descent of heart and lungs.

Month 3-6 - lungs appear glandular, end month 6 alveolar cells type 2 appear and begin to secrete surfactant.

Month 7 - respiratory bronchioles proliferate and end in alveolar ducts and sacs.

Molecular Signaling  
Early development is all about "branching". Fibroblast Growth Factors from the surrounding mesoderm acting through membrane receptors and Sox transcription factors are key regulators of early endodermal branching (Sox2 for initiation, Sox9 for tip maintenance and branching)
Lung human and mouse Sox expression.jpg

Human and mouse Sox expression[1]

Bmp4 acts as in an autocrine signalling mechanism to limit bud outgrowth.

Development Stages

Note - the sequence is important rather than the actual timing, which is variable in the existing literature.

Human Lung Stages
Lung Stage Human Features Vascular
Embryonic week 4 to 5 lung buds originate as an outgrowth from the ventral wall of the foregut where lobar division occurs extra pulmonary artery then lobular artery
Pseudoglandular week 5 to 17 conducting epithelial tubes surrounded by thick mesenchyme are formed, extensive airway branching Pre-acinar arteries
Canalicular week 16 to 25 bronchioles are produced, increasing number of capillaries in close contact with cuboidal epithelium and the beginning of alveolar epithelium development Intra-acinar arteries
Saccular week 24 to 40 alveolar ducts and air sacs are developed alveolar duct arteries
Alveolar late fetal to 8 years secondary septation occurs, marked increase of the number and size of capillaries and alveoli alveolar capillaries
embryonic stage - pseudoglandular stage - canalicular stage - saccular stage - alveolar stage   Links: Species Stage Comparison | respiratory

Embryonic

Week 4 to 5 - lung buds originate as an outgrowth from the ventral wall of the foregut where lobar division occurs.

Endoderm development cartoon
Stage11 bf9.jpg Stage11 sem4.jpg
Stomodeum (Week 4, stage 11) Buccopharyngeal membrane (Week 4, stage 11)

Week 5 Respiratory Development

(Week 5, stage 14)

  • week 4 - 5
  • Endoderm - tubular ventral growth from foregut pharynx.
  • Mesoderm - mesenchyme of lung buds.
  • Intraembryonic coelom - pleural cavities elongated spaces connecting pericardial and peritoneal spaces.

Stage 13 - Trachea and Lung buds

Gray0982a.jpg Bailey287.jpg Bailey288.jpg Bailey289.jpg
Week 4 Week 4-5 (Stage 12 to 13) Week 5 (Stage 15 to 16) Week 6 (Stage 16 to 17)

Pseudoglandular stage

  • week 5 - 17
  • tubular branching of the human lung airways continues
  • by 2 months all segmental bronchi are present.
  • lungs have appearance of a glandlike structure.
  • stage is critical for the formation of all conducting airways.
    • lined with tall columnar epithelium
    • more distal structures are lined with cuboidal epithelium.
  • Pulmonary neuroendocrine cells (PNECs) - develop in late embryonic to early fetal period, later in mid-fetal period clusters of these cells form neuroepithelial bodies (NEBs) in airway epithelium. May stimulate mitosis to increase branching, secrete 2 peptides - gastrin-releasing peptide (GRP) and calcitonin gene related peptide (CGRP)

ME54 001.jpg

Week 8

Fetal lung histology.jpg

Fetal lung histology

(This is what a gland looks like.)

Canalicular stage

  • week 16 - 24
  • Lung morphology changes dramatically
  • differentiation of the pulmonary epithelium results in the formation of the future air-blood tissue barrier.
  • Surfactant synthesis and the canalization of the lung parenchyma by capillaries begin.
  • future gas exchange regions can be distinguished from the future conducting airways of the lungs.
Lung - canalicular stage.jpg

Saccular stage

Alveolar sac structure
  • week 24 to near term.
  • most peripheral airways form widened "airspaces", termed saccules.
  • saccules widen and lengthen the airspace (by the addition of new generations).
  • future gas exchange region expands significantly.
  • Fibroblastic cells also undergo differentiation, they produce extracellular matrix, collagen, and elastin.
    • May have a role in epithelial differentiation and control of surfactant secretion.
  • Alveolar Cells Type II (Type II pneumocytes)
    • begin to secrete surfactant, levels of secretion gradually increase to term.
    • allows alveoli to remain inflated
  • Vascular tree - also grows in length and diameter during this time.

Alveolar stage

Secondary septum
  • late fetal to 8 years.
  • Secondary septum
  • Expansion of gas exchange alveoli, vascular beds (capillaries), lymphatics and innervation.
  • Postnatal lung, with alveoli forming.
Postnatal alveoli number.jpg

Upper Respiratory Tract

Head arches cartoon.jpg Pharynx cartoon.jpg
Foregut cartoon Pharynx

Foregut Development - from the oral cavity the next portion of the foregut is initially a single gastrointestinal (oesophagus) and respiratory (trachea) common tube, the pharynx which lies behind the heart. Note that the respiratory tract will form from a ventral bud arising at this level.

Pharynx
  • part of foregut development (Oral cavity, Pharynx (esophagus, trachea), Respiratory tract, Stomach)
  • anatomically the nose, nasal cavity and the pharynx
  • pharynx forms a major arched cavity within the pharyngeal arches (MH - pharyngeal arches will be described in BGD head development lecture).
  • palate - development for mammals, allows breathing while feeding.

Note - Specialised olfactory epithelium for smell, a small region located in roof of nasal cavity.

Respiratory histology 11.jpg

Respiratory epithelium

  • pseudo-stratified
  • ciliated cells
  • goblet cells
  • basal cells

Respiratory epithelium cells cartoon.jpg

Respiratory epithelium development


Additional Information - Histology
This will be covered in detail in your associated SH Practical class.
Olfactory epithelium
  • Olfactory cells
  • Sustentacular cells - located mainly in the superficial cell layer of the epithelium (difficult to distinguish from olfactory cells).
  • Basal cells - identified by their location in the epithelium.

Epithelium

  • Cilia are not visible
  • goblet cells are absent from the olfactory epithelium.

Lamina Propria

  • olfactory axon bundles (lightly stained, rounded areas) connected to olfactory cells.
  • Bowman's glands - (small mucous glands, olfactory glands) function to moisturise the epithelium.
Nasal Olfactory Histology: overview image | detail image | Smell Development | Histology | Histology Stains


Respiratory epithelium
  • goblet cells
  • ciliated cells
  • basal cells

Lamina propria

  • connective tissue
  • cavernous sinusoids - large spaces (empty or filled with red blood cells)
  • glandular tissue - mucous glands (green) and muco-serous glands (brownish-green)

Bone

  • Lamellae and osteocytes in lacunae.
  • Haversian systems are rare or absent.
Nasal Respiratory Histology: overview image | detail image | Histology | Histology Stains
Respiratory Histology: Bronchiole | Alveolar Duct | Alveoli | EM Alveoli septum | Alveoli Elastin | Trachea 1 | Trachea 2 | labeled lung | unlabeled lung | Respiratory Bronchiole | Lung Reticular Fibres | Nasal Inferior Concha | Nasal Respiratory Epithelium | Olfactory Region overview | Olfactory Region Epithelium | Histology Stains


Lower Respiratory Tract

Lung development stage13-22.jpg Stage 22 image 171.jpg
Stage 13 (Week 4-5) Stage 22 (Week 8)
Lung alveoli development cartoon
  • lung buds ( endoderm epithelial tubes) grow/push into mesenchyme covered with pleural cells (lung border)
  • generates a tree-like network by repeated:
  1. elongation
  2. terminal bifurcation
  3. lateral budding

Growth initially of branched "conducting" system of bronchial tree, followed by later development of the "functional units" of the alveoli.

Additional Information - Histology
This will be covered in detail in your associated SH Practical class.

Respiratory Trachea

Mucosa - formed by epithelium and underlying lamina propria.

  • respiratory epithelium - (pseudostratified columnar and ciliated) ciliated cells, goblet cells, brush cells, endocrine cells, surfactant-producing cells (Clara cells), serous cells, basal cells, basement membrane.
  • lamina propria - loose connective tissue, many elastic fibres

Submucosa - connective tissue and submucosal glands

  • submucosal glands (both serous and mucous parts)

Cartilage

  • perichondrium
  • tracheal cartilage - hyaline cartilage, 16 to 20 C-shaped cartilages.
  • trachealis muscle - (smooth muscle) Not visible in this section, together with connective tissue fibres, join ends of the cartilages together.

Hyaline Cartilage Development

  • forms from mesenchymal cells.
  • precursor cells become rounded and form densely packed cellular masses, chondrification centres.
  • chondroblasts - (cartilage-forming cells) begin secreting the extracellular matrix components of cartilage.
    • extracellular matrix - ground substance (hyaluronan, chondroitin sulfates and keratan sulfate) and tropocollagen (polymerises into fine collagen fibres, not visible).

Bronchi Branching

main bronchi -> lobar bronchi -> segmental bronchi (supply lung bronchopulmonary segments) -> bronchi -> bronchioles (smaller than 1 mm) -> respiratory bronchioles.

  • Trachea branches into 2 main bronchi, with a histological structure similar to that of the trachea.
  • branches are accompanied by branches of the pulmonary artery, nerves and lymph vessels
  • surrounded by a layer of smooth muscle, which is located between the cartilage and epithelium.

Bronchioles

  • transition from bronchi to bronchioles the epithelium changes to a ciliated columnar epithelium.
  • Smooth muscle present, glands and cartilage are absent.

Respiratory Bronchioles

  • first structures that belong to the respiratory portion of the respiratory system.
  • wall out-pouchings form alveoli (site of gas exchange)
  • end in alveolar ducts
  • alveoli - duct or sac.
Alveolar type I cells
  • small alveolar cells or type I pneumocytes
  • are extremely flattened (the cell may be as thin as 0.05 µm)
  • form the bulk (95%) of the surface of the alveolar walls.
  • The shape of the cells is very complex, and they may actually form part of the epithelium on both faces of the alveolar wall.
Alveolar type II cells
  • large alveolar cells or type II pneumocytes
  • about as many type II cells as type I cells (cell shape accounts for small contribution to alveolar area).
  • irregularly (sometimes cuboidal) shaped.
  • form small bulges on the alveolar walls.
  • contain are large number of granules called cytosomes (or multilamellar bodies)
    • consist of precursors to pulmonary surfactant (mixture of phospholipids that keep surface tension in the alveoli low).
Respiratory Histology: Bronchiole | Alveolar Duct | Alveoli | EM Alveoli septum | Alveoli Elastin | Trachea 1 | Trachea 2 | labeled lung | unlabeled lung | Respiratory Bronchiole | Lung Reticular Fibres | Nasal Inferior Concha | Nasal Respiratory Epithelium | Olfactory Region overview | Olfactory Region Epithelium | Histology Stains


Fetal Lung Volume

Each human lung volume as determined by ultrasound and matched to gestational age [2]

Weeks (gestational) Volume (ml)
12 to 13 0.05
19 to 22 0.5
29 to 32 1.9
Lung volume graph 01.jpg

Pleural Cavity

pleura
pleura
  • anatomical body cavity in which the lungs develop and lie.
  • pleural cavity forms in the lateral plate mesoderm as part of the early single intraembryonic coelom.
  • This cavity is initially continuous with pericardial and peritoneal cavities and form initially as two narrow canals.
    • later becomes separated by folding (pleuropericardial fold, pleuroperitoneal membrane) and the later formation of the diaphragm.
  • pleuropericardial fold - (pleuropericardial membrane) An early embryonic fold which restricts the communication between pleural cavity and pericardiac cavity, contains both the cardinal vein and phrenic nerve.
  • pleuroperitoneal membrane - An early embryonic membrane that forms inferiorly at the septum transversum to separate peritoneal cavity from pleural cavity.

Pleura

  • serous membrane covers the surface of the lung and the spaces between the lobes.
  • arranged as a closed invaginated sac.
  • two layers (pulmonary, parietal) continuous with each other, the potential space between them is the pleural cavity.

Diaphragm

  • Not respiratory tract but musculoskeletal development, there are 5 embryonic elements that contribute to the diaphragm.
Components of the diaphragm
  1. septum transversum- central tendon
  2. 3rd to 5th somite- musculature of diaphragm
  3. ventral pleural sac- connective tissue
  4. mesentry of oesophagus- connective tissue around oesophasus and IVC
  5. pleuroperitoneal membranes- connective tissue around central tendon
Adult Cervical Plexus (phrenic nerve shown lower right)

adult diaphragm

  • Innervation of the human diaphragm is by the phrenic nerves
    • arising from the same segmental levels from which the diaphragm skeletal muscles arise, segmental levels C3 to C5.
  • The paired phrenic nerves are mixed nerves
    • motor neurons for the diaphragm
    • sensory nerves for other abdominal structures (mediastinum, pleura, liver, gall bladder).

Bochdalek hernia - most common on the posterior left side (85%). Failure of the pleuroperitoneal foramen (foramen of Bochdalek) to close allows viscera into thorax. Intestine, stomach or spleen can enter the pleural cavity, compressing the lung.

Pulmonary Circulation

Pulmonary circulation
  • the pulmonary system not "functional" until after birth
  • pulmonary arteries - 6th aortic arch arteries
  • pulmonary veins - are incorporated into the left atrium wall
  • bronchial arteries - branches from dorsal aorta

Fetal

Fetal Respiratory Movements

  • Fetal respiratory movements (FRM) or Fetal breathing movements (FBM) are regular muscular contrations occurring in the third trimester.
  • preparing the respiratory muscular system for neonatal function.
  • may also have a role in late lung development.

The First Breath

Alveolar sac structure
  • The respiratory system does not carry out its physiological function (gas exchange) prenatally and remain entirely fluid-filled until birth.
  • At birth, fluid in the upper respiratory tract is expired and fluid in the lung aveoli is rapidly absorbed this event has also been called "dewatering of the lung".
    • The lung epithelia has to now rapidly change from its prenatal secretory function to that of fluid absorbtion.

The exchange of lung fluid for air leads to:

  • fall in pulmonary vascular resistance
  • increase in pulmonary blood flow
  • thinning of pulmonary arteries (stretching as lungs increase in size)
  • blood fills the alveolar capillaries

In the heart - pressure in the right side of the heart decreases and pressure in the left side of the heart increases (more blood returning from pulmonary).

Postnatal

Postnatal alveoli number
Rib orientation

Alveoli

  • At birth about 15% of adult alveoli number have formed
    • 20 - 50 million to in the adult about 300 million.
  • remaining subdivisions develop in the first few postnatal years

Alveoli Number

Respiratory Rate

  • neonatal rate is higher (30-60 breaths/minute) than adult (12-20 breaths/minute).
    • tachypnea - (Greek, rapid breathing) an increased respiratory rate of greater than 60 breaths/minute in a quiet resting baby
Age Rate (breaths/minute)
Infant (birth - 1 year) 30 - 60
Toddler (1 - 3 years) 24 - 40
Preschool (3 - 6 years) 22 - 34
School age (6 - 12 years) 18 - 30
Adolescent (12 - 18 years) 12 - 16

Rib Orientation

  • Infant rib - is virtually horizontal, allowing diaphragmatic breathing only.
  • Adult rib - is oblique (both anterior and lateral views), allows for pump-handle and bucket handle types of inspiration.

Respiratory Tract Abnormalities

Congenital diaphragmatic hernia
Lung Azygos Lobe
Preterm Birth

Respiratory System - Abnormalities

  • Meconium Aspiration Syndrome - (MAS) Meconium is the gastrointestinal contents that accumulate in the intestines during the fetal period. Fetal stress in the third trimester, prior to/at/ or during parturition can lead to premature meconium discharge into the amniotic fluid and sunsequent ingestion by the fetus and damage to respiratory function. Damage to placental vessels meconium myonecrosis may also occur.
  • Newborn Respiratory Distress Syndrome - (Hyaline Membrane Disease) membrane-like substance from damaged pulmonary cells, absence of surfactant, if prolonged can be irreversible, intrauterine asphyxia, prematurity and maternal diabetes medline plus | eMedicine
  • Tracheoesophageal Fistula - Tracheo-Oesophageal Fistula, Oesophageal Atresia - Oesophageal Atresia with or without tracheo-oesophageal fistula Fistula - an abnormal communication between 2 structures (organs, vessels, cavities) that do not normally connect.
  • Lobar Emphysema (Overinflated Lung) - There is an overinflated left upper lobe There is a collapsed lower lobe The left lung is herniating across the mediastinum
  • Congenital Diaphragmatic Hernia - (1 in 3,000 live births) Failure of the pleuroperitoneal foramen (foramen of Bochdalek) to close (left side), allows viscera into thorax -iIntestine, stomach or spleen can enter the pleural cavity, compressing the lung. rare (Morgagni hernia) -an opening in the front of the diaphragm. Congenital Diaphragmatic Hernia | GeneReviews
  • Azygos Lobe - Common condition (0.5% of population). The right lung upper lobe expands either side of the posterior cardinal. There is also some course variability of the phrenic nerve in the presence of an azygos lobe.
  • Congenital Laryngeal Webs - Laryngeal abnormality due to embryonic (week 10) incomplete recanalization of the laryngotracheal tube during the fetal period. Rare abnormality occuring mainly at the level of the vocal folds (glottis).
  • Hyaline Membrane Disease - (Newborn Respiratory Distress Syndrome) a membrane-like substance from damaged pulmonary cells.
  • Bronchopulmonary Dysplasia - A chronic lung disease which can occur following premature birth and related lung injury. Most infants who develop BPD are born more than 10 weeks before their due dates, weigh less than 1,000 grams (about 2 pounds) at birth, and have breathing problems.
  • Asthma - Flow limitation during tidal expiration in early life significantly associated with the development of physician-diagnosed asthma by the age of 2 years. Infants with abnormal lung function soon after birth may have a genetic predisposition to asthma or other airway abnormalities that predict the risk of subsequent lower respiratory tract illness. PMID 8176553
  • Cystic Fibrosis - Inherited disease of the mucus and sweat glands, causes mucus to be thick and sticky. Clogging the lungs, causing breathing problems and encouraging bacterial grow. (Covered elsewhere in the course)
  • Environmental Factors see recent review below. <pubmed>20444669</pubmed>

Additional Information

Respiratory Terms (expand to view) 
  • adenovirus - A Class I virus containing a single double-stranded DNA (dsDNA), which can cause infections in the upper respiratory tract in many animals. (More? viral infection)
  • alveolar duct - Anatomical short region lying between the end of the respiratory bronchioles and the final alveolar sacs. Term is also used in the mammary gland, to describe the smallest of the intralobular ducts into which the secretory alveoli open.
  • alveolar sac - (alveolus), Latin alveolus = little cavity) Anatomical and functional end of the mammalian lung respiratory tree where gas exchange occurs. In humans, during lung development these are the last features to form from 7 months onwards.
  • alveolar - Term used in relation to the alveoli of the lungs. The final functional sac of the respiratory tree where gas exchange occurs between the alveolar space and the pulmonary capillaries.
  • alveolar stage - Term used to describe lung development, the final histological/developmental stage (Pseudoglandular, Fetal Canalicular, Terminal sac, Alveolar). This stage occurs from late fetal/neonate with alveoli formation, the final functional sac of the respiratory tree exists, where gas exchange occurs between the alveolar space and the pulmonary capillaries. (embryonic stage - pseudoglandular stage - canalicular stage - saccular stage - alveolar stage)
  • alveolus - (alveolar sacs, plural alveoli, Latin alveolus = little cavity) Anatomical and functional end of the mammalian lung respiratory tree where gas exchange occurs. In humans, during lung development these are the last features to form from 7 months onwards. The acinus starts approximately 3 to 4 generations proximal of the bronchioalveolar duct junction and ends about 4 generations of alveolar ducts distal of the bronchioalveolar duct junction.
  • angiogenesis - vascular growth by direct extension from pre-existing blood vessels. (see also vasculogenesis).
  • apgar - Non-invasive clinical test designed by Dr Virginia Apgar (1953) carried out immediately on newborn. The name is also an acronym for: Activity (Muscle Tone), Pulse, Grimace (Reflex Irritability), Appearance (Skin Color), Respiration. A score is given for each sign at one minute and five minutes after the birth. (More? Apgar test)
  • apnea - Respiratory term meaning the cessation of breathing.
  • assisted ventilation - Clinical term referring to newborn (perinatal) respiration assistance required immediately following delivery, the infant given minimal breaths for any duration with bag and mask or bag and endotracheal tube within the first several minutes from birth. Excludes free flow oxygen only and laryngoscopy for aspiration of meconium.
  • asthma - Flow limitation during tidal expiration in early life significantly associated with the development of physician-diagnosed asthma by the age of 2 years. Infants with abnormal lung function soon after birth may have a genetic predisposition to asthma or other airway abnormalities that predict the risk of subsequent lower respiratory tract illness. Asthma phenotypes have a number of different classifications; allergic asthma, intrinsic or nonallergic asthma, infectious asthma, and aspirin-exacerbated asthma, and environmental exposures (occupational agents, smoking, air pollution, cold dry air) (More? PMID 5439356)
  • azygos lobe - Common condition (0.5% of population). The right lung upper lobe expands either side of the posterior cardinal. There is also some course variability of the phrenic nerve in the presence of an azygos lobe.
  • Bochdalek hernia - The most common form (80-85%) of the Congenital Diaphragmatic Hernia (CDH) types occurring mainly on the postero-lateral (left) side of the respiratory diaphragm. (More? congenital diaphragmatic hernia)
  • bronchi - (Latin, bronchos = windpipe) Plural of bronchus, the two subdivisions of the trachea carrying air to the lungs. Embryologically form as an endodermal outpocket of the foregut which branch (bronchiole, subdivision of the bronchus) as they grow. Airway: trachea - bronchi - lobar bronchi - segmental bronchi - bronchioles - conducting bronchioles - terminal bronchioles - respiratory bronchioles - alveolar ducts.
  • bronchiole - A smaller branch subdivision of the respiratory tract bronchus, lack supporting cartilage skeletons and have a diameter of about 1 mm. Epithelium is initially ciliated and graduates to simple columnar epithelium and lining no longer contain mucous-producing cells.
  • bronchopulmonary dysplasia - (chronic lung disease in preterm infants) Clinical term for a heterogeneous lung disease seen in preterm (premature) infants and diagnosed within the first months of life. Condition was first described in 1967. (More? preterm birth American Lung Association)
  • canalicular stage - (fetal canalicular, canalicular phase) Term used to describe lung development, after early embryonic the second of the histological/developmental stages (pseudoglandular, fetal canalicular, terminal sac, alveolar). This stage occurs during the fetal period from week 16 to 24. During this stage there is lung bud mesenchymal angiogenesis and cellular differentiation into different stromal cell types (fibroblasts, myoblasts and chondrocytes). (embryonic stage - pseudoglandular stage - canalicular stage - saccular stage - alveolar stage)
  • carbon monoxide - (CO) A colourless and odorless gas formed mainly as a by-product of incomplete combustion of hydrocarbons and can cause cytotoxicity by tissue hypoxia. Carbon monoxide enters circulation though the respiratory system, binding to haemoglobin to form carboxy-haemoglobin (COHb), with fetal haemoglobin binding with a greater affinity.
  • CDH - Acronym for Congenital Diaphragmatic Hernia, a musculoskeletal abnormality of the respiratory diaphragm. The most common form being the Bochdalek hernia.
  • chorioamnionitis - (amnionitis, intra-amniotic infection) intrauterine bacterial infection/inflammation that can cause preterm birth and affect respiratory development directly as well as thought the underdeveloped brainstem, resulting in reduced respiratory drive.
  • chronic lung disease - (CLD) Clinical term, a neonatal chronic lung disease can be caused by prolonged mechanical ventilation (MV) and oxygen-rich gas with premature infants.
  • Clara cells - Respiratory tract epithelial cells on the luminal surface of airways. These cells have a dome shaped cytoplasmic protrusion and no cilia and their function is secretory and xenobiotic. Clara cells can act as progenitor cell in small airways replacing injured terminally differentiated epithelial cells.
  • Clara cell secretory protein - (CCSP) A protective lung protein secreted from non-ciliated bronchiolar epithelial cells in the conducting airways of mammals. The protein increases in expression level post-natally and is thought to have antioxidant, immunomodulatory, and anticarcinogenic properties.
  • connective tissue fibers - form a continuous alveolar support with axial, peripheral and septal fibers.
  • congenital diaphragmatic hernia - Abnormality due to failure of the pleuroperitoneal foramen (foramen of Bochdalek) to close (left side), allows viscera into thorax Intestine, stomach or spleen can enter the pleural cavity, compressing the lung. Rarer (Morgagni hernia) is an opening in the front of the diaphragm. (More? congenital diaphragmatic hernia | GeneReviews
  • congenital laryngeal webs - Laryngeal abnormality due to embryonic (week 10) incomplete recanalization of the laryngotracheal tube during the fetal period. Rare abnormality occuring mainly at the level of the vocal folds (glottis).
  • corticosteroid - An endocrine steroidal hormone produced by the adrenal cortex. Clinically, corticosteroids are also used for lung maturation of the premature neonate.
  • cystic fibrosis - Inherited disease of the mucus and sweat glands, causes mucus to be thick and sticky. Clogging the lungs, causing breathing problems and encouraging bacterial grow. (Covered elsewhere in the course)
  • diaphragm - A general term for a membranous sheet, used to describe the respiratory diaphragm. The muscular sheet separating chest from abdomen with several different embryonic origins. Regular contraction of the diaphragm is required in respiration. The diaphragm forms initially at the lower end of the pleuroperitoneal canal. (Embryonic origins: transverse septum (septum transversum) - tendon of the diaphragm, 3rd to 5th somite pairs - musculature of diaphragm, ventral pleural sac - connective tissue, mesentry of oesophagus - connective tissue around oesophasus and inferior vena cava, and pleuroperitoneal membranes - connective tissue around central tendon)
  • endoderm - (Greek, endo = inside + derma = skin) One of the initial 3 germ cell layers (ectoderm, mesoderm, endoderm) formed by the process of gastrulation. The endoderm forms the epithelial lining glands and of the respiratory tract.
  • epaxial muscle - Anatomical term describing skeletal muscles which lie dorsal (posterior) to the vertebral column developing from the somite myotome. At the ribcage level the levatores costarum muscles involved with rib elevation during respiration.
  • epiglottis - (Greek, epi = above, upon) cartilaginous part of the larynx above the glottis, which in infancy directs food into the esophagus and not the trachea . Embryologically it develops in the foregut from the hypobranchial eminence, behind the undeveloped tongue, from which it separates at about 7 weeks. Postnatal anatomical development in humans involves a maturational descent in infancy (4 and 6 months of age). Contains lymphoid tissue (larynx-associated lymphoid tissue, LALT and Bronchus-associated lymphoid tissue, BALT).
  • Extracorporeal Membrane Oxygenation - (ECMO) an invasive therapy that has been investigated and utilized in newborn infants with cardiorespiratory failure.
  • fetal breathing movements - (FBM) Occur in the third trimester preparing both the skeletomuscular and neural system, and lungs mechanically and the amount of liquid within the developing lungs.
  • fistula - An abnormal communication between 2 structures (organs, vessels, cavities) that do not normally connect, can occur between the trachea and oesophagus.
  • foregut - The first of the three part/division (foregut - midgut - hindgut) of the early forming gastrointestinal tract. The foregut runs from the buccopharyngeal membrane to the midgut and forms all the tract (esophagus and stomach) from the oral cavity to beneath the stomach. In addition, a ventral bifurcation of the foregut will also form the respiratory tract epithelium.
  • glottis - (Greek, = larynx) the boundary between pharynx to the larynx and consists of the vocal folds and their associated intervening space.
  • HIF-1 - A transcription factor that is one of the main regulators of homeostasis in human tissues exposed to hypoxia, due to inflammation and/or insufficient circulation.
  • hyaline membrane disease - (Newborn Respiratory Distress Syndrome) Abnormality due to a membrane-like substance from damaged pulmonary cells.
  • hypopharynx - connects the oropharynx to the oesophagus and the larynx, the region of pharynx below the hyoid bone.
  • laryngeal cleft - (LC, laryngeal-tracheo-oesophageal cleft) A rare foregut abnormality allowing digestive tract and the airway to communicate causing chronic cough, aspiration and respiratory distress. The downward extension of the cleft determines the classification of the abnormality.
  • laryngeal webs - (congenital laryngeal webs) Laryngeal abnormality due to embryonic (week 10) incomplete recanalization of the laryngotracheal tube. Rare abnormality occuring mainly at the level of the vocal folds (glottis).
  • laryngotracheal groove - Early embryonic foregut developmental feature, forms on the anterior (ventral) wall of the pharynx and gives rise to larynx, trachea and entire respiratory tree. In humans, this feature is the first indication of respiratory development and appears during week 4.
  • larynx - Site of the the vocal folds in the neck. Embryologically develops from the foregut with the lining derived from endoderm and the cartilage from pharyngeal arch 4 and 6. Beginning as a simple foregut groove, the laryngotracheal groove which folds to form the laryngotracheal bud, then the larynx and trachea.
  • late-gestation lung protein 1 - (LGL1) A glycoprotein secreted by fetal lung mesenchyme and fetal kidney, involved in retinoic acid stimulated branching morphogenesis.
  • lipofibroblast - (lipid interstitial cell, pulmonary lipofibroblast) Cell involved in secondary septum formation during the alveolar stage of lung development (late fetal to postnatal). Cell is recognizable by a number of characteristic lipid droplets and contains cortical contractile filaments.
  • lobar emphysema - (overinflated lung) Abnormality of an overinflated left upper lobe There is a collapsed lower lobe The left lung is herniating across the mediastinum.
  • measles - (paramyxovirus) Measles (rubeola) is mainly a respiratory viral infection, clinically different from Rubella.
  • meconium aspiration syndrome - (MAS) Fetal stress in the third trimester, prior to/at/ or during parturition can lead to premature meconium discharge into the amniotic fluid and sunsequent ingestion by the fetus and damage to respiratory function.
  • medullary respiratory centres - medulla oblongata collection of nuclei organised into ventral and dorsal respiratory groups. The ventral respiratory nuclei pre-Bötzinger complex (pBÖTC) required for respiratory rhythmogenesis.
  • mitochondria - Double membraned cell organelle located in the cytoplasm, a cell may contain 100's or more mitochondria, the number can relate to the metabolic activity of that cell. Functions in cell respiration, providing energy to the cell and also has a role in the process of apoptosis (programmed cell death).
  • newborn respiratory distress syndrome - (respiratory distress syndrome, RDS, hyaline membrane syndrome) - surfactant deficiency at birth more common in preterm birth. RDS Info
  • nitrofen - A diphenyl ether herbicide teratogen used in rodent development to generate a range of developmental abnormalities, including congenital diaphragmatic hernia.
  • oropharynx - The second portion of the pharynx (throat) connecting the nasopharynx and laryngopharynx (hypopharynx). Region between the palate and the hyoid bone, anteriorly divided from the oral cavity by the tonsillar arch.
  • parathyroid hormone-related protein - (PTHrP) A protein named for its evolutionary and structural relationship to parathyroid hormone (PTH). A protein hormone produced by many fetal tissues and with a number of different functions including a possible autocrine role in lung development.
  • parietal pleura - Serous membrane which forms the outer lining of pleural cavity. mesoderm of the thoracic cavity body wall and derived from epithelia of pericardioperitoneal canals from intra-embryonic coelom. The inner pleural layer, visceral pleura, is splanchnic mesoderm in origin.
  • Pentalogy of Cantrell - A developmental abnormality of the anterior diaphragm, diaphragmatic pericardium, abdominal wall, cardiovascular and lower sternum.
  • Persistent Pulmonary Hypertension of the Newborn - (PPHN) A serious newborn condition due to due to the failure of closure one of the prenatal circulatory shunts, the ductus arteriosus. Occurs in about 1-2 newborns per 1000 live births and results in hypoxemia.
  • pharynx - (throat) embryo uppermost end of the combined gastrointestinal and respiratory tract beginning at the buccopharyngeal membrane and forms a major arched cavity within the phrayngeal arches. Also used as a respiratory term describing the initial segment of the upper respiratory tract divided anatomically into three regions: nasopharynx, oropharynx, and laryngopharynx (hypopharynx). Anatomically extends from the base of the skull to the level of the sixth cervical vertebra.
  • pleural cavity - Anatomical body cavity in which the lungs develop and lie. Forms in the lateral plate mesoderm as part of the early single intra-embryonic coelom, the pleural cavities are initially two narrow canals.
  • pleuropericardial fold - (pleuropericardial membrane) An early embryonic fold which restricts the communication between pleural cavity and pericardiac cavity, contains both the |cardinal vein and phrenic nerve.
  • pleuroperitoneal foramen - The developmental opening occurring in the intra-embryonic coelom before formation of the pleuroperitoneal membrane.
  • PLUNC - Acronym for Palate, LUng, Nasal epithelium Clone protein, related to the lipid transfer/lipopolysaccharide binding protein (LT/LBP) family. This protein is secreted by the airway conducting epithelia and acts as a surfactant that may interfere with biofilm formation by airway pathogens.
  • pulmonary acini (singular - acinus) region of the lung supplied with air from one of the terminal bronchioles, anatomical and functional end of the mammalian lung respiratory tree where gas exchange occurs. Starts approximately 3 to 4 generations proximal of the bronchio-alveolar duct junction and ends about 4 generations of alveolar ducts distal of the bronchioalveolar duct junction.
  • pulmonary arterial hypertension - (PAH) a progressive disease characterized by abnormalities of vascular tone and reactivity, vessel wall structure, growth, and thrombosis that in newborns, infants, and children can contribute to poor outcomes in cardiac, pulmonary, and systemic diseases.
  • respiratory - Term used in relation to breathing (in and out) or associated with the lungs. Anatomically used to describe the lungs, air pathways and associated muscles. In cell biology used in relation to mitochondrial use of oxygen to produce energy and carbon dioxide waste.
  • respiratory bronchioles - may contain alveoli and have surface surfactant-producing Respiratory bronchioles can contain alveoli and surfactant-producing cells, and give rise to between 2 to 11 alveolar ducts.
  • respiratory sinus arrhythmia - (RSA) Clinically used as an index of cardiac vagal activity, measured breath-by-breath by subtracting the minimum heart rate (HR) during expiration from the maximum HR during inspiration.
  • respiratory tree - Anatomical term to describe the components of the respiratory system (lungs) as they branch again and again ending in the functional units, the alveolar sacs (alveolus).
  • saccular stage - (terminal sac stage) process of lung epithelial cell differentiation, vascular remodeling and thinning of the mesenchyme. This process leads to enlargement of the diameter and surface area of the alveolar sacs. Distal epithelial cells form 2 populations: 1. cells flattens, thins, and spreads to form type I cells; 2. cells remain cuboidal, acquire surfactant filled lamellar bodies and differentiate into type II cells. Sacculation is a general anatomical term meaning to formed a series of sac-like expansions. (embryonic stage - pseudoglandular stage - canalicular stage - saccular stage - alveolar stage)
  • secondary septa - (secondary septa) process in the lung alveolar stage of development (postnatally) where the double capillary network in the immature gas-exchange region fuses to form a single capillary layer.
  • septum transversum - (transverse septum) A mesodermal region in the early embryo. Identified externally as the junctional site between amnion and yolk sacs, and internally (within the embryo) lying directly beneath the heart and at the foregut/midgut junction. This ventro-dorsal "plate" of mesoderm contributes several structures including: the central tendon of diaphragm and some of the liver.
  • stenosis - Term used to describe an abnormal narrowing, usually in relation to a tube for example: respiratory tract, blood vessel, gastrointestinal tract.
  • stomodeum - (stomadeum, stomatodeum) The primordial mouth region of the developing embryonic head.
  • surfactant - (surface active agent ; pulmonary surfactant) A mixture of lipids and proteins secreted by Type 2 alveolar cells between alveolar epithelium that reduces surface tension (detergent) at the air-liquid interface. The function is to prevent collapse of the lung at the end of expiration. In humans, these cells and their secretion develop towards the very end of the third trimester, just before birth. Clinical surfactants used for surfactant replacement therapy are animal-derived preparations, commonly bovine (beractant, bovactant, BLES) or less common porcine (butantan, poractant-α and surfacen) PMID30728009.
  • surfactant protein D - (SP-D) a multimeric collectin (collagen-containing C-type lectin) involved in innate immunity (anti-microbial) and expressed in pulmonary and non-pulmonary epithelia. PMID 29473039
  • surfactant replacement therapy - (surfactant therapy) A clinical birth term referring to the endotracheal instillation of a surface-active suspension for treating surfactant deficiency due to either preterm birth or pulmonary injury resulting in respiratory distress (newborn respiratory distress syndrome).
  • tachypnea - (Greek, tachypnea = rapid breathing) Clinical term describing an increased respiratory rate of greater than 60 breaths/minute in a quiet resting baby.
  • terminal sac stage - (saccular stage, terminal sac phase, immature alveoli) Term used to describe the second last histological/developmental stage (pseudoglandular stage, Fetal Canalicular, saccular stage, Alveolar) of lung development. This stage occurs from late fetal week 24 to 36. During this stage branching and growth of the terminal sacs occurs, with cellular differentiation of the type -II pneumonocytes and type - I pneumonocytes The final functional sac of the respiratory tree occurs at the next neonatal period, where gas exchange occurs between the alveolar space and the pulmonary capillaries. (embryonic stage - pseudoglandular stage - canalicular stage - saccular stage - alveolar stage)
  • trachea - (windpipe) In the embryo, a ventral out-pocket of pharynx endoderm that branches in week 4 stage 13 into the right and left bronchi within the lung buds. The endoderm has associated mesoderm that later differentiates to form most structures outside the respiratory epithelium. In the adult, the trachea forms the functional connection between the pharynx and larynx to the lungs. Adult trachea is a ciliated pseudostratified columnar epithelium supported by C-shaped rings of hyaline cartilage.
  • tracheoesophageal fistula - Abnormal connection between the trachea and oesophagus.
  • Trisomy 21 - (Down syndrome) associated with significant cardiovascular and pulmonary mortality and morbidity of neonates, infants, and children. Infant lung histology may share features of decreased lung vascular and alveolar growth. (More? Trisomy 21 | PMID 25621156)
  • vagus - (Latin, vagus = wandering) cranial nerve X (CN X) A mixed nerve that leaves the head and neck to innervate respiratory tract (larynx, lungs), gastrointestinal tract (pharynx, esophagus, stomach), cardiac (heart) and abdominal viscera. This mixed nerve has sensory, motor and autonomic functions of viscera (glands, digestion, heart rate).
  • vasculogenesis - the formation of new blood vessels from angioblasts or endothelial progenitor cells (EPCs) that migrate, differentiate, and grow into tubes in response to signals from surrounding cells. (see also angiogenesis).
  • VEGF - (vascular endothelial growth factor) a specific mitogen and survival factor required for endothelium growth and development also required in lung vasculature development and remodelling.
  • ventilatory unit - region from a respiratory bronchiole extending to the supported alveolar ducts and alveoli.
  • visceral pleura - Serous membrane which forms the inner lining of pleural cavity, both covering and attached to the lungs. Embryonically derived from the splanchnic mesoderm. The outer pleural layer, parietal pleura, is derived from mesoderm of the thoracic cavity body wall.
Other Terms Lists  
Terms Lists: ART | Birth | Bone | Cardiovascular | Cell Division | Endocrine | Gastrointestinal | Genital | Genetic | Head | Hearing | Heart | Immune | Integumentary | Neonatal | Neural | Oocyte | Palate | Placenta | Radiation | Renal | Respiratory | Spermatozoa | Statistics | Tooth | Ultrasound | Vision | Historic | Drugs | Glossary


adult lungs
Grays - Respiratory Images  
Respiratory Histology
Fetal Histology
Fetal Respiratory: late canalicular | unlabeled late canalicular | Hyaline cartilage | Respiratory Histology
Adult Histology
Respiratory Histology: Bronchiole | Alveolar Duct | Alveoli | EM Alveoli septum | Alveoli Elastin | Trachea 1 | Trachea 2 | labeled lung | unlabeled lung | Respiratory Bronchiole | Lung Reticular Fibres | Nasal Inferior Concha | Nasal Respiratory Epithelium | Olfactory Region overview | Olfactory Region Epithelium | Histology Stains


  1. <pubmed>28806170</pubmed>
  2. <pubmed>16388511</pubmed>



Glossary Links

Glossary: A | B | C | D | E | F | G | H | I | J | K | L | M | N | O | P | Q | R | S | T | U | V | W | X | Y | Z | Numbers | Symbols | Term Link
 2018 ANAT2341 - Timetable | Course Outline | Moodle | Tutorial 1 | Tutorial 2 | Tutorial 3

Labs: 1 Preimplantation and Implantation | 2 Reproductive Technology Revolution | 3 Group Projects | 4 GM manipulation mouse embryos | 5 Early chicken eggs | 6 Female reproductive tract | 7 Skin regeneration | 8 Vertebral development | 9 Organogenesis Lab | 10 Cardiac development | 11 Group projects | 12 Stem Cell Journal Club

Lectures: 1 Introduction | 2 Fertilization | 3 Week 1/2 | 4 Week 3 | 5 Ectoderm | 6 Placenta | 7 Mesoderm | 8 Endoderm | 9 Research Technology | 10 Cardiovascular | 11 Respiratory | 12 Neural crest | 13 Head | 14 Musculoskeletal | 15 Limb | 16 Renal | 17 Genital | 18 Endocrine | 19 Sensory | 20 Fetal | 21 Integumentary | 22 Birth | 23 Stem cells | 24 Revision

 Student Projects: Group Projects Information Project 1 | Project 3 | Project 4 | Project 5 | 2018 Test Student | Copyright