Lecture - Fertilization
|Embryology - 22 Sep 2021 Expand to Translate|
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This lecture and the associated laboratory will cover male and female gametogenesis and fertilisation.
Development is 1 embryonic cell producing about 1013 (100,000,000,000,000) cells in the adult at any one time (over time with cell death and ongoing replacement this is substantially more).
This is where the first embryonic cell begins! Fertilization is the fusion of haploid gametes, egg (oocyte) and sperm (spermatozoa), to form the diploid zygote. Note though there can be subtle differences in the fertilization process which occurs naturally within the body or through reproductive technologies outside the body, the overall product in both cases is a diplod zygote.
- Broad understanding of reproductive cycles.
- Understand the key features of gametogenesis.
- Understand the differences in male and female gametogenesis.
- Brief understanding of the differences between mitosis and meiosis.
- Understanding of the events in fertilization.
|The following chapter links only work with a UNSW connection.|
|The following chapter links only work with a UNSW connection.|
Human Reproductive Cycle
Sexual reproduction in most species is regulated by regular endocrine changes, or cycles, in the female. These cycles begin postnatally, function for variable times and can then decrease or cease entirely.
- Human reproduction is regulated in females by the menstrual cycle, a regular cyclic hormonal change which coordinate changes in the ovary and internal reproductive tract. This cycle commences at puberty and ends at menopause.
- Non-primates (rats, mice, horses, pig) reproduction is regulated in females by the estrous cycle (British spelling, oestrous).
Male - Spermatogenesis
Human spermatozoa (electron microscope)
Mouse spermatozoa (electron microscope)
The testes have two functions.
- produce the male gametes or spermatozoa
- produce male sexual hormone, testosterone (internal and external genitalia, sex characteristics)
Human spermatozoa take about 48 days from entering meiosis until morphologically mature spermatozoa.
Female - Oogenesis
The ovaries have two functions.
- produce the female gametes or oocytes
- produce female hormones, estrogen and progesterone (secondary sex characteristics, menstrual cycle)
In an adult human female the development of a primordial follicle containing an oocyte to a preovulatory follicle takes in excess of 120 days.
Human Follicle Development
Human ovary follicle development
Follicle cells (support cells) Theca cells (produce hormone)
- Meiosis initiated continuously in a mitotically dividing stem cell population
- 4 gametes produced / meiosis
- Meiosis completed in days or weeks
- Meiosis and differentiation proceed continuously without cell cycle arrest
- Differentiation of gamete occurs while haploid after meiosis ends
- Sex chromosomes excluded from recombination and transcription during first meiotic prophase
- Meiosis initiated once in a finite population of cells
- 1 gamete produced / meiosis
- Completion of meiosis delayed for months or years
- Meiosis arrested at 1st meiotic prophase and reinitiated in a smaller population of cells
- Differentiation of gamete occurs while diploid in first meiotic prophase
- All chromosomes exhibit equivalent transcription and recombination during meiotic prophase
- In female gametogenesis only a single (1) haploid egg is produced from meiosis. In male gametogenesis four (4) haploid sperm are produced from meiosis. So what happens to all the extra DNA in producing this single egg?
- In Meiosis 1 the "extra" DNA is excluded to the periphery as a 1st polar body, which encloses the extra DNA.
- In Meiosis 2 the "extra" DNA is once again excluded as a 2nd polar body. The first polar body may also under go meiosis 2 producing a 3rd polar body.
- These polar bodies are not gametes.
- Polar bodies appear to have no other function other than to dispose of the extra DNA in oogenesis.
- Recent research in mice suggest that the position of oocyte polar body may influence fertilization site.
|Meiosis Polar Body Movie|
Gamete formation, menstrual cycle and fertilisation will also be covered in detail in this week's Laboratory. Fertilization is the complete process resulting in the fusion of haploid gametes, egg and sperm, to form the diploid zygote. The recent development of aided fertilization is described as in vitro fertilization (in vitro = "in glass", outside the body, IVF). Clinically, all these aided fertilization techniques are grouped as Assisted Reproductive Technologies or ART.
- Oogenesis - 1 gamete produced/meiosis + 3 polar bodies, meiosis is slow, 1 egg produced and released at ovulation
- Spermatogenesis - 4 gametes produced/meiosis, meiosis is fast, 200-600 million sperm released at ejaculation
- Fertilization resulting in embryo development usually occurs in first 1/3 of uterine tube (oviduct, Fallopian tube)
- The majority of fertilized oocytes do not go on to form an embryo
- Fertilization can also occur outside uterine tube associated with Assisted Reproductive Technologies (IVF, GIFT, ZIFT...) and ectopic pregnancy
- Oocyte ovulation - release from the ovary with associated cells, into peritoneal cavity, uterine tube fimbria then into uterine tube (oviduct, uterine horn, fallopian tube) and epithelial cilia mediated movement.
- Spermatozoa ejaculation - deposited in vagina, movement of tail to "swim" in uterine secretions through cervix, uterine body and into uterine tube, have approximately 24-48h to fertilize oocyte.
Prior to the fertilization process commencing both the gametes complete of a number of biological processes.
- Oocyte Meiosis - completes Meiosis 1 and commences Meiosis 2 (arrests at Metaphase II).
- Spermatozoa Capacitation - following release (ejaculation) and mixing with other glandular secretions, activates motility and acrosome preparation.
- Migration - both oocyte and spermatozoa.
|Gamete Movement Movies|
Fertilization - Male
Spermatozoa: Ejaculation - Capacitation - Spermatozoa motility - Chemotaxis - Binding to zona pellucida - Acrosome reaction - Membrane fusion
- about 3.5 ml, containing 200 - 600 million spermatozoa
- by volume less than 10 % spermatozoa
- accessory glands contribute majority of volume (60 % seminal vesicle, 10 % bulbourethral, 30 % prostate)
- Oligospermia (Low Sperm Count) - less than 20 million sperm after 72 hour abstinence from sex
- Azoospermia (Absent Sperm) - blockage of duct network
- Immotile Cilia Syndrome - lack of sperm motility
- spermatozoa activation process - removal of glycoprotein coat and seminal proteins and alteration of sperm mitochondria
- tail of spermatozoa provide movement by microtubules
- energy for this movement is provided by mitochondria in tail initial segment
- oocyte cumulus cells release progesterone (may also be other oocyte and follicular fluid factors)
- Zona pellucida protein ZP2 acts as receptor for spermatozoa binding (species specific)
- exocytosis of acrosome contents (calcium mediated) MBoC - Figure 20-31. The acrosome reaction that occurs when a mammalian sperm fertilizes an egg
- enzymes to digest the zona pellucida
- exposes sperm surface proteins to bind ZP2
- between spermatozoa and oocyte cell membranes, allows sperm nuclei passage into egg cytoplasm
- membrane fusion also initiates oocyte processes to block polyspermy
Fertilization - Oocyte
Oocyte: Membrane depolarization - Cortical reaction - Meiosis 2 completion
- caused by spermatozoa membrane fusion, acts as primary block to polyspermy (fertilisation by more than one spermatozoa)
- Inositol triphosphate (IP3) pathway elevates intracellular calcium, exocytosis of cortical granules
- enzyme alters ZP2 so it will no longer bind sperm plasma membrane
- MBoC - Figure 20-32. How the cortical reaction in a mouse egg is thought to prevent additional sperm from entering the egg
- completion of 2nd meiotic division
- forms second polar body (third polar body may be formed by meiotic division of the first polar body)
Formation of the Zygote
|Human Zygote||Mouse Zygote|
- Pronuclei - Male and Female haploid nuclei approach each other and nuclear membranes break down
- chromosomal pairing, DNA replicates, first mitotic division
- Sperm contributes - centriole which organizes mitotic spindle
- Oocyte contributes - mitochondria (maternally inherited)
- based upon whether an X or Y carrying sperm has fertilized the egg, should be 1.0 sex ratio.
- actually 1.05, 105 males for every 100 females, some studies show more males 2+ days after ovulation.
- cell totipotent (equivalent to a stem cell, can form any tissue of the body)
Men - Y Chromosome
- Y Chromosome carries Sry gene, protein product activates pathway for male gonad (covered in genital development)
Women - X Chromosome
- Gene dosage, one X chromosome in each female embryo cell has to be inactivated
- process is apparently random and therefore 50% of cells have father's X, 50% have mother's X
- Note that because men only have 1 X chromosome, if abnormal, this leads to X-linked diseases more common in male that female where bothe X's need to be abnormal.
- The most common chromosome abnormality is aneuploidy, the gain or loss of whole chromosomes.
- Caused by meiotic nondisjunction, the failure of chromosomes to correctly separate homologues during meiosis I or sister chromatids during meiosis II.
- Down Syndrome - caused by an extra copy of chromosome 21. Abnormal Development - Trisomy 21 (Down Syndrome) Maternal Age
- Chromosomal translocations occur when there is an inappropriate exchange of chromosomal material. Philadelphia chromosome
- Philadelphia chromosome - piece of Chr9 exchanged with Chr22 Generates truncated abl, overstimulates cell production, leads to chronic myelogenous leukemia
- Complete Mole - Only paternal chromosomes.
- Partial Mole - 3 sets of chromosomes ( (triploidy) instead of the usual 2.
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UNSW Embryology Links
|Cell Division Links: meiosis | mitosis | Lecture - Cell Division and Fertilization | spermatozoa | oocyte | fertilization | zygote | Genetics|
- Developmental Biology by Gilbert, Scott F. Sunderland (MA): Sinauer Associates, Inc.; c2000Figure 2.9. Summary of meiosis | fusion of egg and sperm plasma membranes
- Molecular Biology of the Cell 4th ed. Alberts, Bruce; Johnson, Alexander; Lewis, Julian; Raff, Martin; Roberts, Keith; Walter, Peter New York and London: Garland Science; c2002 - IV. Internal Organization of the Cell Chapter 17. The Cell Cycle and Programmed Cell Death Programmed Cell Death | An Overview of the Cell Cycle | Figure 17-1. The cell cycle | Fertilization
- Molecular Cell Biology by Lodish, Harvey; Berk, Arnold; Zipursky, S. Lawrence; Matsudaira, Paul; Baltimore, David; Darnell, James E. New York: W. H. Freeman & Co.; c1999 Chapter 13. Regulation of the Eukaryotic Cell Cycle Regulation of the Eukaryotic Cell Cycle | Overview of the Cell Cycle and Its Control | Figure 13-2. Current model for regulation of the eukaryotic cell cycle | Movies Proposed alternative mechanisms for chromosome congression. | Centromeric attachment of microtubules. | The stages of mitosis and cytokinesis in an animal cell.
- The Cell - A Molecular Approach by Cooper, Geoffrey M. Sunderland (MA): Sinauer Associates, Inc.; c2000- IV. Cell Regulation Chapter 14. The Cell Cycle The Eukaryotic Cell Cycle | Figure 14.1. Phases of the cell cycle | Figure 14.32. Comparison of meiosis and mitosis | Figure 14.37. Meiosis of vertebrate oocytes
- HSTAT - In Vitro Fertilization As A Medical Treatment For Male or Female Infertility
- MBoC MBoC - Figure 20-18. Influence of Sry on gonad development
- Endocrinology Endocrinology - Comparative anatomy of male and female reproductive tracts
- asthenozoospermia - (asthenospermia) Term for reduced sperm motility and can be the cause of male infertility.
- blood-testis barrier - (BTB) Formed by tight junctions, basal ectoplasmic specializations, desmosome-like junctions and gap junctions between adjacent Sertoli cells near the basement membrane of the seminiferous epithelium.
- diploid - (Greek, di = double + ploion = vessel) Having two sets of chromosomes, the normal state for all cells other than the gametes.
- haploid - (Greek, haploos = single) Having a single set of chromosomes as in mature germ/sex cells (oocyte, spermatozoa) following reductive cell division by meiosis. Normally cells are diploid, containing 2 sets of chromosomes.
- Leydig cell - (interstitial cell) Male gonad (testis) cell which secrete the androgen testosterone, beginning in the fetus. These cells are named after Franz von Leydig (1821 - 1908) a German scientist who histologically described these cells.
- meiosis - The cell division that occurs only in production of germ cells where there is a reduction in the number of chromosomes (diploid to haploid) which is the basis of sexual reproduction. All other non-germ cells in the body divide by mitosis.
- mitosis - The normal division of all cells, except germ cells, where chromosome number is maintained (diploid). In germ cell division (oocyte, spermatozoa) meiosis is a modified form of this division resulting in reduction in genetic content (haploid). Mitosis, division of the nucleus, is followed by cytokinesis the division of the cell cytoplasm and the cytoplasmic contents. cytokinesis overlaps with telophase.
- sperm annulus - (Jensen's ring; Latin, annulus = ring) A region of the mammalian sperm flagellum connecting the midpiece and the principal piece. The annulus is a septin-based structure formed from SEPT1, 4, 6, 7 and 12. Septins are polymerizing GTPases that can act as a scaffold forming hetero-oligomeric filaments required for cytokinesis and other cell cycle roles.
- spermatogenesis - (Greek, genesis = origin, creation, generation) The term used to describe the process of diploid spermatagonia division and differentiation to form haploid spermatazoa within the testis (male gonad). The process includes the following cellular changes: meiosis, reoorganization of DNA, reduction in DNA content, reorganization of cellular organelles, morphological changes (cell shape). The final process of change in cell shape is also called spermiogenesis.
- spermatogenesis - (Greek, genesis = origin, creation, generation) The maturation process of the already haploid spermatazoa into the mature sperm shape and organization. This process involves reorganization of cellular organelles (endoplasmic reticulum, golgi apparatus, mitochondria), cytoskeletal changes (microtubule organization) and morphological changes (cell shape, acrosome and tail formation).
- spermatogonia - The cells located in the seminiferous tubule adjacent to the basal membrane that either divide and separate to renew the stem cell population, or they divide and stay together as a pair (Apr spermatogonia) connected by an intercellular cytoplasmic bridge to differentiate and eventually form spermatazoa.
- spermatozoa head - Following spermiogenesis, the first region of the spermatozoa containing the haploid nucleus and acrosome. In humans, it is a flattened structure (5 µm long by 3 µm wide) with the posterior part of nuclear membrane forming the basal plate region. The human spermatozoa is about 60 µm long, actively motile and divided into 3 main regions (head, neck and tail).
- spermatozoa neck - Following spermiogenesis, the second region of the spermatozoa attached to basal plate, transverse oriented centriole, contains nine segmented columns of fibrous material, continue as outer dense fibres in tail. In humans, it forms a short structure (1 µm). The human spermatozoa is about 60 µm long, actively motile and divided into 3 main regions (head, neck and tail).
- spermatozoa tail - Following spermiogenesis, the third region of the spermatozoa that has a (head, neck and tail). The tail is also divided into 3 structural regions a middle piece, a principal piece and an end piece. In humans: the middle piece (5 µm long) is formed by axonema and dense fibres surrounded by mitochondria; the principal piece (45 µm long) fibrous sheath interconnected by regularly spaced circumferential hoops; the final end piece (5 µm long) has an axonema surrounded by small amount of cytoplasm and plasma membrane.
- spermatogonial stem cells - (SSCs) The spermatagonia cells located beside the seminiferous tubule basal membrane that either divide and separate to renew the stem cell population, or they divide and stay together as a pair (Apr spermatogonia) connected by an intercellular cytoplasmic bridge to differentiate and eventually form spermatazoa.
- sperm protein 56 - A component of the spermatozoa acrosomal matrix released to the sperm surface during capacitation.
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- JCB - Movie Collection Mitosis | Cytokinesis
- 2008 ANAT3231 Lecture PDF lecture14-15 1 slide/page (view) | 4 slides/page (print) | 6 slides/page (print) | text (print)
- McGraw-Hill Animation comparing Mitosis and Meiosis
- Salmon Lab Mitosis Movies
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Cite this page: Hill, M.A. (2021, September 22) Embryology Lecture - Fertilization. Retrieved from https://embryology.med.unsw.edu.au/embryology/index.php/Lecture_-_Fertilization
- © Dr Mark Hill 2021, UNSW Embryology ISBN: 978 0 7334 2609 4 - UNSW CRICOS Provider Code No. 00098G