Implantation microRNA: Difference between revisions
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==Introduction== | |||
[[File:Mark_Hill.jpg|thumb|Dr Mark Hill]] | |||
[[File:Acps-logo.png|thumb|Australian Centre for Perinatal Science]] | |||
Ectopic pregnancy is a high-risk maternal medical condition with an approximate incidence of 1.5 to 2 % in reported pregnancies. In the USA, data from commercial health plans identified 11,989 ectopic pregnancies during the period from 2002 to 2007.<ref name=PMID20177279><pubmed>20177279</pubmed></ref> In the Australian state of New South Wales, in 2008 the annual hospitalisation rate for women with an ectopic pregnancy was 12.6 per 1000 births.<ref name=PMID22877595><pubmed>22877595</pubmed></ref> | |||
This is new collaborative research project between clinical researchers from the Women and Childrens Hospital (RHW), basic researchers from the School of Medical Sciences (SoMS), the Australian Centre for Perinatal Science (ACPS) and research support from the HSA Biobank and Bioanalytical Mass Spectrometry Facility ( BMSF). | |||
Our research group has established a uterine tube Biobank and cell culture techniques in order to investigate factors and markers associated with tubal ectopic pregnancies. | |||
This page introduces the project and provides some introductory information for students who may wish to carry out an Honours, Independent Research Project or higher degree in the research laboratory. [[#Student Ectopic Implantation Projects|Student Projects]] | [[#Biomedical Research Expo 2014|2014 Biomed Expo]] | |||
:'''Research Links:''' [[ACPS_Seminar_2014_-_Implantation|2014 Seminar]] | [https://research.unsw.edu.au/people/dr-mark-hill Dr Mark Hill] | [https://research.unsw.edu.au/people/professor-william-ledger Professor William Ledger] | [http://www.seslhd.health.nsw.gov.au/rhw/ Royal Hospital for Women] | [http://medicalsciences.med.unsw.edu.au SOMS] | [http://www.acps.unsw.edu.au ACPS] | [http://www.tcrn.unsw.edu.au/hsa HSA Biobank] | [http://www.bmsf.unsw.edu.au BMSF] | |||
:'''Ectopic Page Links:''' [[Abnormal Development - Ectopic Implantation|Ectopic Implantation]] | [[Implantation]] | [[Week 2]] | [[Placenta - Abnormalities]] | [[Trophoblast]] | [[Trophoblast - Protein Expression]] | [[Implantation microRNA]] | |||
==Some Recent Findings== | |||
[[File:Tubal_pregnancy.gif|thumb|Tubal Pregnancy]] | |||
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! More recent papers | |||
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| [[File:Mark_Hill.jpg|90px|left]] {{Most_Recent_Refs}} | |||
Search term: [http://www.ncbi.nlm.nih.gov/pubmed/?term=Ectopic+Pregnancy ''Ectopic Pregnancy''] | |||
<pubmed limit=5>Ectopic Pregnancy</pubmed> | |||
Search term: [http://www.ncbi.nlm.nih.gov/pubmed/?term=Implantation+microRNA ''Implantation microRNA''] | |||
<pubmed limit=5>Implantation microRNA</pubmed> | |||
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===miRNA signature and Dicer requirement during human endometrial stromal decidualization in vitro=== | ===miRNA signature and Dicer requirement during human endometrial stromal decidualization in vitro=== | ||
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PMID 22911744 | PMID 22911744 | ||
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Revision as of 15:00, 2 March 2016
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Introduction
Ectopic pregnancy is a high-risk maternal medical condition with an approximate incidence of 1.5 to 2 % in reported pregnancies. In the USA, data from commercial health plans identified 11,989 ectopic pregnancies during the period from 2002 to 2007.[1] In the Australian state of New South Wales, in 2008 the annual hospitalisation rate for women with an ectopic pregnancy was 12.6 per 1000 births.[2]
This is new collaborative research project between clinical researchers from the Women and Childrens Hospital (RHW), basic researchers from the School of Medical Sciences (SoMS), the Australian Centre for Perinatal Science (ACPS) and research support from the HSA Biobank and Bioanalytical Mass Spectrometry Facility ( BMSF).
Our research group has established a uterine tube Biobank and cell culture techniques in order to investigate factors and markers associated with tubal ectopic pregnancies.
This page introduces the project and provides some introductory information for students who may wish to carry out an Honours, Independent Research Project or higher degree in the research laboratory. Student Projects | 2014 Biomed Expo
- Research Links: 2014 Seminar | Dr Mark Hill | Professor William Ledger | Royal Hospital for Women | SOMS | ACPS | HSA Biobank | BMSF
- Ectopic Page Links: Ectopic Implantation | Implantation | Week 2 | Placenta - Abnormalities | Trophoblast | Trophoblast - Protein Expression | Implantation microRNA
Some Recent Findings
More recent papers |
---|
This table allows an automated computer search of the external PubMed database using the listed "Search term" text link.
More? References | Discussion Page | Journal Searches | 2019 References | 2020 References Search term: Ectopic Pregnancy <pubmed limit=5>Ectopic Pregnancy</pubmed> Search term: Implantation microRNA <pubmed limit=5>Implantation microRNA</pubmed> |
miRNA signature and Dicer requirement during human endometrial stromal decidualization in vitro
PLoS One. 2012;7(7):e41080. doi: 10.1371/journal.pone.0041080. Epub 2012 Jul 20.
Estella C1, Herrer I, Moreno-Moya JM, Quiñonero A, Martínez S, Pellicer A, Simón C.
Abstract
Decidualization is a morphological and biochemical transformation of endometrial stromal fibroblast into differentiated decidual cells, which is critical for embryo implantation and pregnancy establishment. The complex regulatory networks have been elucidated at both the transcriptome and the proteome levels, however very little is known about the post-transcriptional regulation of this process. miRNAs regulate multiple physiological pathways and their de-regulation is associated with human disorders including gynaecological conditions such as endometriosis and preeclampsia. In this study we profile the miRNAs expression throughout human endometrial stromal (hESCs) decidualization and analyze the requirement of the miRNA biogenesis enzyme Dicer during this process. A total of 26 miRNAs were upregulated and 17 miRNAs downregulated in decidualized hESCs compared to non-decidualized hESCs. Three miRNAs families, miR-181, miR-183 and miR-200, are down-regulated during the decidualization process. Using miRNAs target prediction algorithms we have identified the potential targets and pathways regulated by these miRNAs. The knockdown of Dicer has a minor effect on hESCs during in vitro decidualization. We have analyzed a battery of decidualization markers such as cell morphology, Prolactin, IGFBP-1, MPIF-1 and TIMP-3 secretion as well as HOXA10, COX2, SP1, C/EBPß and FOXO1 expression in decidualized hESCs with decreased Dicer function. We found decreased levels of HOXA10 and altered intracellular organization of actin filaments in Dicer knockdown decidualized hESCs compared to control. Our results provide the miRNA signature of hESC during the decidualization process in vitro. We also provide the first functional characterization of Dicer during human endometrial decidualization although surprisingly we found that Dicer plays a minor role regulating this process suggesting that alternative biogenesis miRNAs pathways must be involved in human endometrial decidualization.
PMID 22911744
Cite this page: Hill, M.A. (2024, April 25) Embryology Implantation microRNA. Retrieved from https://embryology.med.unsw.edu.au/embryology/index.php/Implantation_microRNA
- © Dr Mark Hill 2024, UNSW Embryology ISBN: 978 0 7334 2609 4 - UNSW CRICOS Provider Code No. 00098G