Histology Artifacts: Difference between revisions
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== Introduction== | == Introduction== | ||
There are many steps in the preparation and processing of tissue sections that can introduce artefacts into the original tissue. This page introduces some of the steps in tissue preparation where this can occur and the type of artefact that appears in the final tissue. | There are many steps in the preparation and processing of tissue sections that can introduce artefacts into the original tissue. This page introduces some of the steps in tissue preparation where this can occur and the type of artefact that appears in the final tissue. The pages on histology {{fixatives}} and {{stains}} should be read in conjunction with this page. | ||
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<center>''In histology, if it looks interesting, it is probably an artefact!''</center> | <center>''In histology, if it looks interesting, it is probably an artefact!''</center> | ||
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Draft page - Notice removed when completed. | |||
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{{Histology Links}} | {{Histology Links}} | ||
==Artifacts== | |||
* Prefixation artifacts | * Prefixation artifacts | ||
* Fixation artifacts | * Fixation artifacts | ||
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* Mounting artifacts | * Mounting artifacts | ||
==Prefixation Artifacts== | |||
Anything that may have been done to the tissue before it has been preserved by fixation. This includes handling, physical distortion, drug treatments or injections, tissue degeneration, or poor tissue preparation for fixation. | |||
==Fixation Artifacts== | |||
Fixative solution osmolality | |||
* Hypertonic can cause cellular shrinkage and increased extracellular spaces. | |||
* Hypotonic can cause cell swelling. | |||
Revision as of 14:08, 12 March 2019
Embryology - 20 Apr 2024 Expand to Translate |
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Introduction
There are many steps in the preparation and processing of tissue sections that can introduce artefacts into the original tissue. This page introduces some of the steps in tissue preparation where this can occur and the type of artefact that appears in the final tissue. The pages on histology fixatives and stains should be read in conjunction with this page.
Draft page - Notice removed when completed.
Artifacts
- Prefixation artifacts
- Fixation artifacts
- Artifacts related to bone tissue
- Tissue-processing artifacts
- Artifacts related to microtomy
- Artifacts related to floatation and mounting
- Staining artifacts
- Mounting artifacts
Prefixation Artifacts
Anything that may have been done to the tissue before it has been preserved by fixation. This includes handling, physical distortion, drug treatments or injections, tissue degeneration, or poor tissue preparation for fixation.
Fixation Artifacts
Fixative solution osmolality
- Hypertonic can cause cellular shrinkage and increased extracellular spaces.
- Hypotonic can cause cell swelling.
Glossary Links
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Cite this page: Hill, M.A. (2024, April 20) Embryology Histology Artifacts. Retrieved from https://embryology.med.unsw.edu.au/embryology/index.php/Histology_Artifacts
- © Dr Mark Hill 2024, UNSW Embryology ISBN: 978 0 7334 2609 4 - UNSW CRICOS Provider Code No. 00098G