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Swapping mitochondrial DNA in mammalian oocytes

Technique has the potential to prevent transmission of human mutated mitochondrial DNA from mother to baby. (figure reference[1])

Working with rhesus macaque monkeys, Tachibana et al.[2]:

  • a - removed the nuclear material plus a cellular membrane (a karyoplast) from a mature oocyte, leaving behind its mitochondria. The nuclear material in the karyoplast consists of condensed chromosomes attached to thread-like spindle fibres (the spindle–chromosomal complex).
  • b - transferred the karyoplast to an oocyte whose nucleus had been removed (a cytoplast).
  • c - fused the karyoplast with the cytoplast and then fertilized the reconstructed oocyte.
  • d - developing blastocyst was implanted in a surrogate mother.
  • e - mother gave birth to a healthy baby.


Links: Mitochondria | Assisted Reproductive Technology

Reference

  1. <pubmed>19759608</pubmed>
  2. <pubmed>19710649</pubmed>

Nature 461, 354-355 (17 September 2009) | doi:10.1038/461354a; Published online 16 September 2009


Original Image Link: http://www.nature.com/nature/journal/v461/n7262/fig_tab/461354a_F1.html



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Licensee: Mark A Hill

License Date: Sep 17, 2009

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Publication: Nature

Title: Developmental biology: Asexual healing

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current12:47, 18 September 2009Thumbnail for version as of 12:47, 18 September 2009700 × 380 (44 KB)S8600021 (talk | contribs)Swapping mitochondrial DNA in mammalian oocytes :"Working with rhesus macaque monkeys, Tachibana et al. removed the nuclear material plus a cellular membrane (a karyoplast) from a mature oocyte, leaving behind its mitochondria (a). They transferred the k