File:Swapping mitochondrial DNA mammalian oocytes.jpg: Difference between revisions

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Swapping mitochondrial DNA in mammalian oocytes

Technique has the potential to prevent transmission of human mutated mitochondrial DNA from mother to baby. (figure reference^[1])

Working with rhesus macaque monkeys, Tachibana et al.^[2]:

a - removed the nuclear material plus a cellular membrane (a karyoplast) from a mature oocyte, leaving behind its mitochondria. The nuclear material in the karyoplast consists of condensed chromosomes attached to thread-like spindle fibres (the spindle–chromosomal complex).
b - transferred the karyoplast to an oocyte whose nucleus had been removed (a cytoplast).
c - fused the karyoplast with the cytoplast and then fertilized the reconstructed oocyte.
d - developing blastocyst was implanted in a surrogate mother.
e - mother gave birth to a healthy baby.

Links: mitochondria | Assisted Reproductive Technology

Reference

↑ Shoubridge EA. (2009). Developmental biology: Asexual healing. Nature , 461, 354-5. PMID: 19759608 DOI.
↑ Tachibana M, Sparman M, Sritanaudomchai H, Ma H, Clepper L, Woodward J, Li Y, Ramsey C, Kolotushkina O & Mitalipov S. (2009). Mitochondrial gene replacement in primate offspring and embryonic stem cells. Nature , 461, 367-72. PMID: 19710649 DOI.

Nature 461, 354-355 (17 September 2009) | doi:10.1038/461354a; Published online 16 September 2009

Original Image Link: http://www.nature.com/nature/journal/v461/n7262/fig_tab/461354a_F1.html

Cite this page: Hill, M.A. (2024, April 20) Embryology Swapping mitochondrial DNA mammalian oocytes.jpg. Retrieved from https://embryology.med.unsw.edu.au/embryology/index.php/File:Swapping_mitochondrial_DNA_mammalian_oocytes.jpg

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current	11:47, 18 September 2009		700 × 380 (44 KB)	S8600021 (talk \| contribs)	Swapping mitochondrial DNA in mammalian oocytes :"Working with rhesus macaque monkeys, Tachibana et al. removed the nuclear material plus a cellular membrane (a karyoplast) from a mature oocyte, leaving behind its mitochondria (a). They transferred the k

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[PMID19759608-1] Shoubridge EA. (2009). Developmental biology: Asexual healing. Nature , 461, 354-5. PMID: 19759608 DOI.

[PMID19710649-2] Tachibana M, Sparman M, Sritanaudomchai H, Ma H, Clepper L, Woodward J, Li Y, Ramsey C, Kolotushkina O & Mitalipov S. (2009). Mitochondrial gene replacement in primate offspring and embryonic stem cells. Nature , 461, 367-72. PMID: 19710649 DOI.

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@@ Line 1: / Line 1: @@
-Swapping mitochondrial DNA in mammalian oocytes
+==Swapping mitochondrial DNA in mammalian oocytes==
+Technique has the potential to prevent transmission of human mutated mitochondrial DNA from mother to baby. (figure reference{{#pmid:19759608|PMID19759608}})
+Working with rhesus macaque monkeys, Tachibana et al.{{#pmid:19710649|PMID19710649}}:
+* '''a''' - removed the nuclear material plus a cellular membrane (a karyoplast) from a mature oocyte, leaving behind its mitochondria. The nuclear material in the karyoplast consists of condensed chromosomes attached to thread-like spindle fibres (the spindle–chromosomal complex).
+* '''b''' - transferred the karyoplast to an oocyte whose nucleus had been removed (a cytoplast).
+* '''c''' - fused the karyoplast with the cytoplast and then fertilized the reconstructed oocyte.
+* '''d''' -  developing blastocyst was implanted in a surrogate mother.
+*  '''e''' - mother gave birth to a healthy baby.
+:'''Links:''' {{mitochondria}} | {{Assisted Reproductive Technology}}
+===Reference===
+<references/>
+Nature 461, 354-355 (17 September 2009) | doi:10.1038/461354a; Published online 16 September 2009
-:"Working with rhesus macaque monkeys, Tachibana et al. removed the nuclear material plus a cellular membrane (a karyoplast) from a mature oocyte, leaving behind its mitochondria (a). They transferred the karyoplast to an oocyte whose nucleus had been removed (a cytoplast; b). The nuclear material in the karyoplast consists of condensed chromosomes attached to thread-like spindle fibres (the spindle–chromosomal complex). The authors fused the karyoplast with the cytoplast and then fertilized the reconstructed oocyte (c). The developing blastocyst (d) was implanted in a surrogate mother, which gave birth to a healthy baby (e). This technique has the potential to prevent transmission of human mutated mitochondrial DNA from mother to baby."
 Original Image Link: http://www.nature.com/nature/journal/v461/n7262/fig_tab/461354a_F1.html
-Rightslink / Nature Publishing Group Order Details
+{{Footer}}
-Licensee: Mark A Hill
-License Date: Sep 17, 2009
+[[Category:Assisted Reproductive Technology]][[Category: Monkey]] [[Category:Mitochondria]]
-License Number: 2271631413678
-Publication: Nature
-Title: Developmental biology: Asexual healing
-Type Of Use: Web Site
-Total: $0.00